Early gene expression in bacteriophage T7. I. In vivo synthesis, inactivation, and translational utilization of early mRNA's.

abstract：:It has been suggested that sequences located within the 5' noncoding region of human foamy virus (HFV) are critical for expression of the viral Gag and Pol structural proteins. Here, we identify a discrete approximately 151-nucleotide sequence, located within the R region of the HFV long terminal repeat, that activate...

journal_title：Journal of virology

authors： Russell RA,Zeng Y,Erlwein O,Cullen BR,McClure MO

更新日期：2001-08-01 00:00:00

abstract：:The catalytic core domain (CCD) of human immunodeficiency virus type 1 (HIV-1) integrase (IN) harbors the enzyme active site and binds viral and chromosomal DNA during integration. Thirty-five CCD mutant viruses were constructed, paying particular attention to conserved residues in the Phe(139)-Gln(146) flexible loop ...

journal_title：Journal of virology

authors： Lu R,Limón A,Ghory HZ,Engelman A

更新日期：2005-02-01 00:00:00

abstract：:Vectors derived from lentiviruses provide a promising gene delivery system. We examined the in vivo gene transfer efficiency and tissue or cell tropism of a feline immunodeficiency virus (FIV)-based lentiviral vector pseudotyped with the glycoproteins from Ross River Virus (RRV). RRV glycoproteins were efficiently inc...

journal_title：Journal of virology

authors： Kang Y,Stein CS,Heth JA,Sinn PL,Penisten AK,Staber PD,Ratliff KL,Shen H,Barker CK,Martins I,Sharkey CM,Sanders DA,McCray PB Jr,Davidson BL

更新日期：2002-09-01 00:00:00

abstract：:Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. In this study, we have analyzed nucleotide sequences encompassing 629 bp at the carboxy terminus of the G glycoprotein gene for HRSV subgr...

journal_title：Journal of virology

authors： Zlateva KT,Lemey P,Vandamme AM,Van Ranst M

更新日期：2004-05-01 00:00:00

abstract：:Kilham rat virus (KRV) contains linear, single-stranded DNA in the virion. The fate of radioactive viral DNA was followed after infection of monolayer cells. Within 60 min after infection of cells, 28 to 42% of the parental viral DNA is converted to a new form. This new DNA form is believed to be double stranded and l...

journal_title：Journal of virology

authors： Salzman LA,White W

更新日期：1973-02-01 00:00:00

abstract：:We previously reported that nucleolin, a representative nucleolar marker, interacts with nonstructural protein 5B (NS5B) of hepatitis C virus (HCV) through two independent regions of NS5B, amino acids 208 to 214 and 500 to 506. We also showed that truncated nucleolin that harbors the NS5B-binding region inhibited the ...

journal_title：Journal of virology

authors： Shimakami T,Honda M,Kusakawa T,Murata T,Shimotohno K,Kaneko S,Murakami S

更新日期：2006-04-01 00:00:00

abstract：:The sole immediate-early (IE) gene of equine herpesvirus 1 encodes a 1,487-amino-acid (aa) regulatory phosphoprotein that independently activates expression of early viral genes. Coimmunoprecipitation assays demonstrated that the IE protein physically interacts with the general transcription factor TFIIB. Using a vari...

journal_title：Journal of virology

authors： Jang HK,Albrecht RA,Buczynski KA,Kim SK,Derbigny WA,O'Callaghan DJ

更新日期：2001-11-01 00:00:00

abstract：:Since parvoviruses apparently do not possess a DNA polymerase activity, one or more of the host cell DNA polymerases must be responsible for replicating the single-stranded DNA genome. We have focused on determining which polymerase, alpha, beta, or gamma (pol alpha, pol beta, or pol gamma, respectively), is responsib...

journal_title：Journal of virology

authors： Robertson AT,Stout ER,Bates RC

更新日期：1984-03-01 00:00:00

abstract：:The three double-stranded ribonucleic acid (dsRNA) segments of the bacteriophage phi6 were isolated and shown to have similar melting temperatures and base compositions. RNA:RNA hybridization experiments with the isolated segments eliminate the possibility that the two smaller dsRNA segments arise from a cleavage of t...

journal_title：Journal of virology

authors： Van Etten JL,Vidaver AK,Koski RK,Burnett JP

更新日期：1974-06-01 00:00:00

abstract：:Recent in vitro proteomics screens revealed that many host proteins could interact with the replication proteins of Tomato bushy stunt virus (TBSV), which is a small, plus-stranded RNA virus (Z. Li, D. Barajas, T. Panavas, D. A. Herbst, and P. D. Nagy, J. Virol. 82:6911-6926, 2008). To further our understanding of the...

journal_title：Journal of virology

authors： Barajas D,Li Z,Nagy PD

更新日期：2009-11-01 00:00:00

abstract：:The processes that facilitate transport of integral membrane proteins though the secretory pathway and subsequently target them to particular cellular membranes are relevant to almost every field of biology. These transport processes involve integration of proteins into the membrane of the endoplasmic reticulum (ER), ...

journal_title：Journal of virology

authors： Brock SC,Heck JM,McGraw PA,Crowe JE Jr

更新日期：2005-10-01 00:00:00

abstract：:In an attempt to identify the molecules involved in the pathogenesis of prion diseases, we performed cDNA subtraction on the brain tissues of mice affected with an experimental prion disease and the unaffected control. The genes identified as being upregulated in the prion-affected brain tissue included those encoding...

journal_title：Journal of virology

authors： Kopacek J,Sakaguchi S,Shigematsu K,Nishida N,Atarashi R,Nakaoke R,Moriuchi R,Niwa M,Katamine S

更新日期：2000-01-01 00:00:00

abstract：:Ebola virus is the etiologic agent of a lethal hemorrhagic fever in humans and nonhuman primates with mortality rates of up to 90%. Previous studies with Zaire Ebola virus (ZEBOV), mouse-adapted virus (MA-ZEBOV), and mutant viruses (ZEBOV-NP(ma), ZEBOV-VP24(ma), and ZEBOV-NP/VP24(ma)) allowed us to identify the mutati...

journal_title：Journal of virology

authors： Cilloniz C,Ebihara H,Ni C,Neumann G,Korth MJ,Kelly SM,Kawaoka Y,Feldmann H,Katze MG

更新日期：2011-09-01 00:00:00

abstract：:The majority of T-cell clones derived from a donor who experienced dengue illness following receipt of a live experimental dengue virus type 3 (DEN3) vaccine cross-reacted with all four serotypes of dengue virus, but some were serotype specific or only partially cross-reactive. The nonstructural protein, NS3, was immu...

journal_title：Journal of virology

authors： Zeng L,Kurane I,Okamoto Y,Ennis FA,Brinton MA

更新日期：1996-05-01 00:00:00

abstract：:We have previously demonstrated that open reading frame (ORF) 50 and ORF 57 encode transcriptional regulating genes in herpesvirus saimiri. ORF 50, a homolog of Epstein-Barr virus R protein, is a sequence-specific transactivator, whereas ORF 57 acts posttranscriptionally. In this report, we demonstrate that the ORF 57...

journal_title：Journal of virology

authors： Whitehouse A,Cooper M,Hall KT,Meredith DM

更新日期：1998-03-01 00:00:00

abstract：:Infection of normal rat fibroblasts (FR 3T3) with the early tsa mutant of polyoma virus may lead to either the A or the N phenotype, tsa-A transformants, originally derived by agar selection, are not temperature dependent for maintenance of the transformed phenotype, whereas tsa-N transormants revert at high temperatu...

journal_title：Journal of virology

authors： Rassoulzadegan M,Seif R,Cuzin F

更新日期：1978-11-01 00:00:00

abstract：:The human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env) encodes specific trafficking signals within its long cytoplasmic tail (CT) that regulate incorporation into HIV-1 particles. Rab11-family interacting protein 1C (FIP1C) and Rab14 are host trafficking factors required for Env particle incorpora...

journal_title：Journal of virology

authors： Kirschman J,Qi M,Ding L,Hammonds J,Dienger-Stambaugh K,Wang JJ,Lapierre LA,Goldenring JR,Spearman P

更新日期：2018-02-12 00:00:00

abstract：:The viral genes UL128, UL130, and UL131A have been identified as major determinants of endothelial cell (EC) tropism of human cytomegalovirus (HCMV), with deletion of either gene causing a null phenotype. We hypothesized that a functional scanning of these genes by minor genetic modifications would allow for the gener...

journal_title：Journal of virology

authors： Schuessler A,Sampaio KL,Sinzger C

更新日期：2008-11-01 00:00:00

abstract：:Nipah virus (NiV) and Hendra virus (HeV) are emerging zoonotic viruses and the causative agents of severe respiratory disease and encephalitis in humans. Little is known about the mechanisms that govern the development of respiratory and neurological disease. Using a hamster model of lethal NiV and HeV infection, we d...

journal_title：Journal of virology

authors： Rockx B,Brining D,Kramer J,Callison J,Ebihara H,Mansfield K,Feldmann H

更新日期：2011-08-01 00:00:00

abstract：:Recently we showed that the membrane-proximal stem region of the vesicular stomatitis virus (VSV) G protein ectodomain (G stem [GS]), together with the transmembrane and cytoplasmic domains, was sufficient to mediate efficient VSV budding (C. S. Robison and M. A. Whitt, J. Virol. 74:2239-2246, 2000). Here, we show tha...

journal_title：Journal of virology

authors： Jeetendra E,Robison CS,Albritton LM,Whitt MA

更新日期：2002-12-01 00:00:00

abstract：:We have obtained a polyclonal antiserum, N-BE, against the denatured, amino-terminal half of the measles virus (MV) nucleocapsid (N) protein and a monoclonal antibody (MAb), N46, which recognizes a conformation-dependent epitope in the same region. Amino acid residues 23 to 239 were required and sufficient for the for...

journal_title：Journal of virology

authors： Gombart AF,Hirano A,Wong TC

更新日期：1993-07-01 00:00:00

abstract：:ICP4 is a herpesvirus-encoded protein that is expressed during the immediate-early phase of productive infection and is required for efficient transcription of viral genes during the early and late phases of infection. Previous studies have shown that ICP4 is a component of specific protein-DNA complexes but have not ...

journal_title：Journal of virology

authors： Kattar-Cooley P,Wilcox KW

更新日期：1989-02-01 00:00:00

abstract：:The effect of human cytomegalovirus infection on cellular DNA synthesis in human fibroblasts was measured by fluorometry and by incorporation of radiolabeled thymidine. The results show that although HCMV infection stimulates cellular DNA synthesis in both quiescent and serum-stimulated cells, radiolabeled thymidine i...

journal_title：Journal of virology

authors： Morin J,Johann S,O'Hara B,Gluzman Y

更新日期：1996-09-01 00:00:00

abstract：:It is generally accepted that the fitness cost of resistance mutations plays a role in the persistence of transmitted drug-resistant human immunodeficiency virus type 1 and that mutations that confer a high fitness cost are less able to persist in the absence of drug pressure. Here, we show that the fitness cost of re...

journal_title：Journal of virology

authors： Cong ME,Heneine W,García-Lerma JG

更新日期：2007-03-01 00:00:00

abstract：:Pseudorabies virus (PRV), a member of the Alphaherpesvirinae, has a complex multilayered extracellular virion that is structurally conserved among other herpesviruses. PRV virions contain a double-stranded DNA genome within a proteinaceous capsid surrounded by the tegument, a layer of viral and cellular proteins. The ...

journal_title：Journal of virology

authors： Kramer T,Greco TM,Enquist LW,Cristea IM

更新日期：2011-07-01 00:00:00

abstract：:HIV-1 infections cannot be completely eradicated by drug therapy, as the virus persists in reservoirs. Low-level plasma viremia has been detected in patients treated for over 7 years, but the cellular compartments that support this low-level viremia have not been identified. The decay of HIV-1 during treatment appears...

journal_title：Journal of virology

authors： Zhang J,Perelson AS

更新日期：2013-07-01 00:00:00

abstract：:The relatively small package capacity (less than 5 kb) of adeno-associated virus (AAV) vectors has been effectively doubled with the development of dual-vector heterodimerization approaches. However, the efficiency of such dual-vector systems is limited not only by the extent to which intermolecular recombination occu...

journal_title：Journal of virology

authors： Yan Z,Zak R,Zhang Y,Engelhardt JF

更新日期：2005-01-01 00:00:00

abstract：:Amino acid analysis of [3H]proline-labeled polyomavirus major capsid protein VP1 by two-dimensional paper chromatography of the acid-hydrolyzed protein revealed the presence of 3H-labeled hydroxyproline. Addition of the proline analog L-azetidine-2-carboxylic acid to infected mouse kidney cell cultures prevented or gr...

journal_title：Journal of virology

authors： Ludlow JW,Consigli RA

更新日期：1989-06-01 00:00:00

abstract：:Alphaviruses represent a serious public health threat and cause a wide variety of diseases, ranging from severe encephalitis, which can result in death or neurological sequelae, to mild infection, characterized by fever, skin rashes, and arthritis. In the infected cells, alphaviruses express only four nonstructural pr...

journal_title：Journal of virology

authors： Gorchakov R,Garmashova N,Frolova E,Frolov I

更新日期：2008-10-01 00:00:00

abstract：:The UL11 tegument protein of herpes simplex virus plays a critical role in the secondary envelopment; however, the mechanistic details remain elusive. Here, we report a new function of UL11 in the budding process in which it directs efficient acquisition of glycoprotein E (gE) via a direct interaction. In vitro bindin...

journal_title：Journal of virology

authors： Han J,Chadha P,Meckes DG Jr,Baird NL,Wills JW

更新日期：2011-09-01 00:00:00