Abstract:
:The development of a reproducible model system for the study of hepatitis C virus (HCV) infection has the potential to significantly enhance the study of virus-host interactions and provide future direction for modeling the pathogenesis of HCV. While there are studies describing global gene expression changes associated with HCV infection, changes in the proteome have not been characterized. We report the first large-scale proteome analysis of the highly permissive Huh-7.5 cell line containing a full-length HCV replicon. We detected >4,200 proteins in this cell line, including HCV replicon proteins, using multidimensional liquid chromatographic (LC) separations coupled to mass spectrometry. Consistent with the literature, a comparison of HCV replicon-positive and -negative Huh-7.5 cells identified expression changes of proteins involved in lipid metabolism. We extended these analyses to liver biopsy material from HCV-infected patients where a total of >1,500 proteins were detected from only 2 mug of liver biopsy protein digest using the Huh-7.5 protein database and the accurate mass and time tag strategy. These findings demonstrate the utility of multidimensional proteome analysis of the HCV replicon model system for assisting in the determination of proteins/pathways affected by HCV infection. Our ability to extend these analyses to the highly complex proteome of small liver biopsies with limiting protein yields offers the unique opportunity to begin evaluating the clinical significance of protein expression changes associated with HCV infection.
journal_name
J Viroljournal_title
Journal of virologyauthors
Jacobs JM,Diamond DL,Chan EY,Gritsenko MA,Qian W,Stastna M,Baas T,Camp DG 2nd,Carithers RL Jr,Smith RD,Katze MGdoi
10.1128/JVI.79.12.7558-7569.2005subject
Has Abstractpub_date
2005-06-01 00:00:00pages
7558-69issue
12eissn
0022-538Xissn
1098-5514pii
79/12/7558journal_volume
79pub_type
杂志文章abstract::Previous analyses have demonstrated that adenovirus DNA is packaged into virions in vivo in a polar, left-to-right fashion. The packaging of viral DNA is dependent on cis-acting elements at the left end of the genome. In this report, we describe a genetic analysis of the sequences that are required for efficient packa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2047-2056.1990
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abstract::The envelope glycoprotein (GP) of lymphocytic choriomeningitis virus (LCMV) is posttranslationally cleaved into two subunits. We show here that this endoproteolytic processing is not required for transport to the cell surface but is essential for LCMV GP to mediate infectivity of pseudotyped retroviral vectors. By sys...
journal_title:Journal of virology
pub_type: 杂志文章
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abstract::ABT-378, a new human immunodeficiency virus type 1 (HIV-1) protease inhibitor which is significantly more active than ritonavir in cell culture, is currently under investigation for the treatment of AIDS. Development of viral resistance to ABT-378 in vitro was studied by serial passage of HIV-1 (pNL4-3) in MT-4 cells....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7532-7541.1998
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.3.944-948.1985
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.12.8051-8056.1995
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.23.12369-12375.2002
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00240-20
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pub_type: 杂志文章
doi:10.1128/JVI.70.12.9041-9045.1996
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4232-4241.1990
更新日期:1990-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01134-14
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02227-07
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.5476-5482.1994
更新日期:1994-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.7.4470-4478.1992
更新日期:1992-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.79.11.7217-7226.2005
更新日期:2005-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.2.220-227.1972
更新日期:1972-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.3.1036-1038.1984
更新日期:1984-12-01 00:00:00
abstract::Activation of host innate immune responses was studied in severe acute respiratory syndrome coronavirus (SCV)-infected human A549 lung epithelial cells, macrophages, and dendritic cells (DCs). In all cell types, SCV-specific subgenomic mRNAs were seen, whereas no expression of SCV proteins was found. No induction of c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.21.13800-13805.2005
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.553-560.1999
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.6.3664-3674.2005
更新日期:2005-03-01 00:00:00
abstract::A fibrosarcoma was induced in a hamster by bovine papilloma virus type 2 (BPV2). The content of BPV2 DNA sequences was measured by DNA-DNA and cRNA-DNA hybridizations. The tumor contained approximately 300 BPV2 genome equivalents per cell. Southern blot hybridization indicated that the viral DNA was in free form, the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.3.945-949.1981
更新日期:1981-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.1.89-97.1991
更新日期:1991-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00051-10
更新日期:2010-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00946-18
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03518-12
更新日期:2013-07-01 00:00:00
abstract::The E5 oncoprotein of bovine papillomavirus type 1 is a 44-amino-acid, hydrophobic polypeptide which localizes predominantly in Golgi membranes and appears to transform cells through the activation of tyrosine kinase growth factor receptors. In fibroblasts, E5 interacts with both the 16-kilodalton vacuolar ATPase subu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.4.2420-2430.1996
更新日期:1996-04-01 00:00:00
abstract::We prepared retrovirus packaging cell lines containing gag-pol genes from spleen necrosis virus (expressed from a cytomegalovirus promoter and the simian virus 40 (SV40) polyadenylation sequences) and, on a separate vector, either the env gene from spleen necrosis virus (expressed from the Rous sarcoma virus promoter ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.7.3209-3212.1989
更新日期:1989-07-01 00:00:00
abstract::When human adenovirus type 2 or 12 infects cells, either productively or non-productively, three classes of viral deoxyribonucleic acid (DNA) are found within the cells: (i) viral DNA which cosediments with DNA extracted from infectious adenovirions at 31.3S for adenovirus type 2 and at 29.0S for adenovirus type 12, (...
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pub_type: 杂志文章
doi:10.1128/JVI.7.6.707-719.1971
更新日期:1971-06-01 00:00:00