Abstract:
:The herpes simplex virus glycoprotein designated VP7(B2) is extracted from virions by nonionic detergent in the form of an oligomer, whereas the other detergent-soluble envelope proteins appear to be extracted as monomers. The subunits of the VP7(B2) oligomer cannot be dissociated by 2-mercaptoethanol and are also resistant to dissociation by a mixture of sodium dodecyl sulfate and 2-mercaptoethanol, except at elevated temperature. The oligomeric form of solubilized VP7(B2) appears to be predominantly dimeric, based on the sedimentation rats in sucrose gradients and the electrophoretic mobilities in sodium dodecyl sulfate-containing acrylamide gels of the undissociated and heat-dissociated forms of VP7(B2).
journal_name
J Viroljournal_title
Journal of virologyauthors
Sarmiento M,Spear PGdoi
10.1128/JVI.29.3.1159-1167.1979subject
Has Abstractpub_date
1979-03-01 00:00:00pages
1159-67issue
3eissn
0022-538Xissn
1098-5514journal_volume
29pub_type
杂志文章abstract::Poliovirus proteins 3A and 3AB are small, membrane-binding proteins that play multiple roles in viral RNA replication complex formation and function. In the infected cell, these proteins associate with other viral and cellular proteins as part of a supramolecular complex whose structure and composition are unknown. We...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02398-10
更新日期:2011-05-01 00:00:00
abstract::Klebsiella pneumoniae MirM7 is a wild-type strain which grows as cocci at pH 7 and above and as rods at pH 6.5 and below. Cultures of this strain and an auxotrophic derivative, MirM7b, have been found to undergo spontaneous lysis after purification from possible contaminating viruses. Lysates always contained two phag...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.3.772-785.1978
更新日期:1978-12-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) is a recently described arterivirus responsible for disease in swine worldwide. Comparative sequence analysis of 3'-terminal structural genes of the single-stranded RNA viral genome revealed the presence of two genotypic classes of PRRSV, represented by the p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.270-280.1999
更新日期:1999-01-01 00:00:00
abstract::Many of the approximately 165 proteins encoded by the African swine fever virus (ASFV) genome do not have significant similarity to known proteins and have not been studied experimentally. One such protein is DP148R. We showed that the DP148R gene is transcribed at early times postinfection. Deletion of this gene did ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01428-17
更新日期:2017-11-30 00:00:00
abstract::Infection and transformation of chondrocytes by Rous sarcoma viruses (RSVs) (Schmidt-Ruppin, Prague) stimulated the rate of cell growth. In contrast, several transformation-defective (td) mutants (tdPRA, tdNY105, tdNY106, tdNY107, and tdNY108) retaining various sizes of the src gene did not stimulate cell growth, indi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.2.531-541.1980
更新日期:1980-08-01 00:00:00
abstract::The pattern of induced protein species of the prototype strain of the parvovirus minute virus of mice was determined in permissive A9 mouse fibroblast cells by high-resolution two-dimensional gel electrophoresis. Identities of the viral proteins in the gels were assigned by probing two-dimensional blots with antisera ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.9.5126-5138.1993
更新日期:1993-09-01 00:00:00
abstract::The membrane fusion events which initiate human immunodeficiency virus type 1 (HIV-1) infection and promote cytopathic syncytium formation in infected cells commence with the binding of the HIV envelope glycoprotein (Env) to CD4 and an appropriate coreceptor. Here, we show that HIV Env-coreceptor interactions activate...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.13.7138-7147.2004
更新日期:2004-07-01 00:00:00
abstract::Synthesis of the vesicular stomatitis virus membrane matrix protein and the glycoprotein is inhibited to a greater extent than the synthesis of the nucleocapsid protein, the nonstructural protein, and the large protein when the rate of peptide chain initiation is reduced by exposure of vesicular stomatitis virus-infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.1.283-286.1976
更新日期:1975-01-01 00:00:00
abstract::Molluscum contagiosum virus (MCV), the only member of the Molluscipoxvirus genus, causes benign papules in healthy people but disfiguring lesions in immunocompromised patients. The sequence of MCV has been completed, revealing that MCV encodes a probable type I topoisomerase enzyme. All poxviruses sequenced to date al...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3401-3406.1998
更新日期:1998-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.18.11837-11847.2005
更新日期:2005-09-01 00:00:00
abstract::In immunocompromised patients, infection with Kaposi's sarcoma-associated herpesvirus (KSHV) can give rise to Kaposi's sarcoma and several lymphoproliferative disorders. In these tumors, KSHV establishes a latent infection in many of the rapidly proliferating and morphologically abnormal cells. Only a few viral gene p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.18.10074-10085.2004
更新日期:2004-09-01 00:00:00
abstract::In this study, we investigated the interaction of reovirus particles with cell membranes by using a 51Cr release assay. We confirmed prior observations (J. Borsa, B. D. Morash, M. D. Sargent, T. P. Copps, P. A. Lievaart, and J. G. Szekely, J. Gen. Virol. 45:161-170, 1979) that intermediate subviral particles (ISVPs) o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.9.5339-5345.1993
更新日期:1993-09-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) evokes a strong immune response, but the virus persists. Polymorphisms within known antigenic sites result in loss of immune recognition and can be positively selected. Amino acid variation outside known HLA class I restricted epitopes can also enable immune escape by interf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00968-06
更新日期:2007-02-01 00:00:00
abstract::We investigated the synergism between influenza virus and Streptococcus pneumoniae, particularly the role of deletions in the stalk region of the neuraminidase (NA) of H2N2 and H9N2 avian influenza viruses. Deletions in the NA stalk (ΔNA) had no effect on NA activity or on the adherence of S. pneumoniae to virus-infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05809-11
更新日期:2012-04-01 00:00:00
abstract::The cytomegalovirus (CMV) serine proteinase assemblin is synthesized as a precursor that undergoes three principal autoproteolytic cleavages. Two of these are common to the assemblin homologs of all herpes group viruses: one at the maturational site near the carboxyl end of the precursor and another at the release sit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.8.5395-5404.1996
更新日期:1996-08-01 00:00:00
abstract:UNLABELLED:The role of NF-κB in influenza A virus (IAV) infection does not reveal a coherent picture, as pro- and also antiviral functions of this transcription factor have been described. To address this issue, we used clustered regularly interspaced short palindromic repeat with Cas9 (CRISPR-Cas9)-mediated genome eng...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00946-16
更新日期:2016-08-12 00:00:00
abstract::Epstein-Barr virus (EBV) is a herpesvirus that infects cells by fusing its lipid envelope with the target cell membrane. The fusion process requires the actions of viral glycoproteins gH, gL, and gB for entry into epithelial cells and additionally requires gp42 for entry into B cells. To further study the roles of the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00572-06
更新日期:2006-10-01 00:00:00
abstract::Serial serum specimens from 22 herpes simplex virus (HSV)-seronegative recipients of an HSV type 2 (HSV-2) glycoprotein subunit vaccine were analyzed by radioimmunoprecipitation and polyacrylamide gel electrophoresis for the development of antibodies to HSV-2 gB, gD, and g80, a complex of gC and gE. Volunteers receive...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.2.475-481.1985
更新日期:1985-11-01 00:00:00
abstract::The rate of transcription in isolated nuclei of primary mouse kidney cells increases by a factor of about 1.5 between 5 and 8 h after infection with polyoma virus or simian virus 40. This process requires intact virus and is inhibited by cycloheximide and actinomycin D. It appears to involve the activity of all three ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.1.8-19.1980
更新日期:1980-07-01 00:00:00
abstract:UNLABELLED:In a genome-wide small interfering RNA (siRNA) screen, we recently identified the interferon (IFN)-inducible protein 35 (IFI35; also known as IFP35) as a factor required for vesicular stomatitis virus (VSV) infection. Studies reported here were conducted to further understand the role and requirement of IFI3...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03202-13
更新日期:2014-03-01 00:00:00
abstract::The mechanism by which herpes simplex virus 1 (HSV-1) establishes latency in sensory neurons is largely unknown. Recent studies indicate that epigenetic modifications of the chromatin associated with the latent genome may play a key role in the transcriptional control of lytic genes during latency. In this study, we f...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00686-09
更新日期:2009-08-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) entry into target cells requires folding of two heptad-repeat regions (N-HR and C-HR) of gp41 into a trimer of N-HR and C-HR hairpins, which brings viral and target cell membranes together to facilitate membrane fusion. Peptides corresponding to the N-HR and C-HR of gp41 are...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.3.1666-1671.2003
更新日期:2003-02-01 00:00:00
abstract::Borna disease virus (BDV) is an enveloped virus with a genome organization characteristic of Mononegavirales. However, based on its unique features, BDV is considered the prototypic member of a new virus family, Bornaviridae, within the order Mononegavirales. We have described the establishment of a reverse genetics s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.10.6544-6550.2005
更新日期:2005-05-01 00:00:00
abstract::The N-terminal domain of the bovine papillomavirus type 1 E2 protein is important for viral DNA replication, for transcriptional transactivation, and for interaction with the E1 protein. To determine which residues of this 200-amino-acid domain are important for these activities, single conservative amino acid substit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.1.23-29.1996
更新日期:1996-01-01 00:00:00
abstract::Despite successful antiretroviral therapy (ART), low-level viremia (LLV) may be intermittently detected in most HIV-infected patients. Longitudinal blood plasma and resting CD4(+) T cells were obtained from two patients on suppressive ART to investigate the source of LLV. Single-genome sequencing of HIV-1 env from LLV...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00284-11
更新日期:2011-05-01 00:00:00
abstract::White spot syndrome virus (WSSV) occurs worldwide and causes high mortality and considerable economic damage to the shrimp farming industry. No adequate treatments against this virus are available. It is generally accepted that invertebrates such as shrimp do not have an adaptive immune response system such as that pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.4.2057-2061.2004
更新日期:2004-02-01 00:00:00
abstract::Understanding the microbiome of ticks in Australia is of considerable interest given the ongoing debate over whether Lyme disease and its causative agent, the bacterium Borrelia burgdorferisensu lato, are present in Australia. The diversity of bacteria infecting Australian ticks has been studied using both culture- an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01358-18
更新日期:2019-01-17 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) gp41 plays a critical role in the viral fusion process, and its N- and C-terminal heptad repeat domains serve as important targets for developing anti-HIV-1 drugs, like T-20 (generic name, enfuvirtide; brand name, Fuzeon). Here, we conducted a yeast two-hybrid screening on a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00644-10
更新日期:2010-09-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) is a large (150-kb) double-stranded DNA virus that forms latent infections in neuronal cells of the human peripheral nervous system. Previous work determined that the HSV-1 genome is found in an ordered nucleosomal structure during latent infection. However, during lytic infection, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.18.10178-10186.2004
更新日期:2004-09-01 00:00:00
abstract::Studies on deoxyribonucleic acid purified from the granulosis virus of Trichoplusia ni have revealed the presence of a closed, double-stranded superhelix which sediments at 95S relative to relaxed circles (74S) and linear (60S) forms. Molecular weight estimates show that this insect virus deoxyribonucleic acid has a s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.4.710-713.1972
更新日期:1972-04-01 00:00:00