Abstract:
:The movement protein (MP) of Tobacco mosaic virus mediates the cell-to-cell transport of viral RNA through plasmodesmata, cytoplasmic cell wall channels for direct cell-to-cell communication between adjacent cells. Previous in vivo studies demonstrated that the RNA transport function of the protein correlates with its association with microtubules, although the exact role of microtubules in the movement process remains unknown. Since the binding of MP to microtubules is conserved in transfected mammalian cells, we took advantage of available mammalian cell biology reagents and tools to further address the interaction in flat-growing and transparent COS-7 cells. We demonstrate that neither actin, nor endoplasmic reticulum (ER), nor dynein motor complexes are involved in the apparent alignment of MP with microtubules. Together with results of in vitro coprecipitation experiments, these findings indicate that MP binds microtubules directly. Unlike microtubules associated with neuronal MAP2c, MP-associated microtubules are resistant to disruption by microtubule-disrupting agents or cold, suggesting that MP is a specialized microtubule binding protein that forms unusually stable complexes with microtubules. MP-associated microtubules accumulate ER membranes, which is consistent with a proposed role for MP in the recruitment of membranes in infected plant cells and may suggest that microtubules are involved in this process. The ability of MP to interfere with centrosomal gamma-tubulin is independent of microtubule association with MP, does not involve the removal of other tested centrosomal markers, and correlates with inhibition of centrosomal microtubule nucleation activity. These observations suggest that the function of MP in viral movement may involve interaction with the microtubule-nucleating machinery.
journal_name
J Viroljournal_title
Journal of virologyauthors
Ferralli J,Ashby J,Fasler M,Boyko V,Heinlein Mdoi
10.1128/JVI.00254-06subject
Has Abstractpub_date
2006-06-01 00:00:00pages
5807-21issue
12eissn
0022-538Xissn
1098-5514pii
80/12/5807journal_volume
80pub_type
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pub_type: 杂志文章
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.7187-7195.1995
更新日期:1995-11-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.00580-12
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journal_title:Journal of virology
pub_type: 临床试验,杂志文章
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更新日期:1999-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00720-17
更新日期:2017-08-10 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.48.1.300-303.1983
更新日期:1983-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.4.3005-3012.1997
更新日期:1997-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.1.20-25.1984
更新日期:1984-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2007-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2019-05-29 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1969-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2016-09-29 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1975-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.1.478-482.1981
更新日期:1981-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.2.593-605.1981
更新日期:1981-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.12.6.1503-1511.1973
更新日期:1973-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.5.1593-1601.1987
更新日期:1987-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1981-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2015-04-01 00:00:00