Abstract:
:Recombinant human metapneumovirus (HMPV) in which the SH, G, or M2 gene or open reading frame was deleted by reverse genetics was evaluated for replication and vaccine efficacy following topical administration to the respiratory tract of African green monkeys, a permissive primate host. Replication of the deltaSH virus was only marginally less efficient than that of wild-type HMPV, whereas the deltaG and deltaM2-2 viruses were reduced sixfold and 160-fold in the upper respiratory tract and 3,200-fold and 4,000-fold in the lower respiratory tract, respectively. Even with the highly attenuated mutants, there was unequivocal HMPV replication at each anatomical site in each animal. Thus, none of these three proteins is essential for HMPV replication in a primate host, although G and M2-2 increased the efficiency of replication. Each gene-deletion virus was highly immunogenic and protective against wild-type HMPV challenge. The deltaG and deltaM2-2 viruses are promising vaccine candidates that are based on independent mechanisms of attenuation and are appropriate for clinical evaluation.
journal_name
J Viroljournal_title
Journal of virologyauthors
Biacchesi S,Pham QN,Skiadopoulos MH,Murphy BR,Collins PL,Buchholz UJdoi
10.1128/JVI.79.19.12608-12613.2005subject
Has Abstractpub_date
2005-10-01 00:00:00pages
12608-13issue
19eissn
0022-538Xissn
1098-5514pii
79/19/12608journal_volume
79pub_type
杂志文章abstract::Monoclonal antibody vpg15 detects a 24-kDa cell surface protein on feline cells permissive for infection with feline immunodeficiency virus (FIV). The antibody blocks infection of FIV-susceptible cells, and expression of the vpg15 marker is decreased in FIV-infected cells in vitro. These results suggest that the antib...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1667-1671.1993
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.3.1093-1096.1988
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pub_type: 杂志文章
doi:10.1128/jvi.76.23.11785-11792.2002
更新日期:2002-12-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1995-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2003-07-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2002-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2047-2056.1990
更新日期:1990-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2016-08-12 00:00:00
abstract::Virion capture assays, in which immobilized antibodies (Abs) capture virus particles, have been used to suggest that nonneutralizing Abs bind effectively to human immunodeficiency virus type 1 (HIV-1) primary viruses. Here, we show that virion capture assays, under conditions commonly reported in the literature, give ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.1.353-365.2003
更新日期:2003-01-01 00:00:00