Abstract:
:In vitro analysis of the catalytic DNA polymerase encoded by vaccinia virus has demonstrated that it is innately distributive, catalyzing the addition of <10 nucleotides per primer-template binding event in the presence of 8 mM MgCl(2) or 40 mM NaCl (W. F. McDonald and P. Traktman, J. Biol. Chem. 269:31190-31197, 1994). In contrast, cytoplasmic extracts isolated from vaccinia virus-infected cells contain a highly processive form of DNA polymerase, able to catalyze the replication of a 7-kb template per binding event under similar conditions. To study this holoenzyme, we were interested in purifying and characterizing the vaccinia virus processivity factor (VPF). Our previous studies indicated that VPF is expressed early after infection and has a native molecular mass of approximately 48 kDa (W. F. McDonald, N. Klemperer, and P. Traktman, Virology 234:168-175, 1997). Using these criteria, we established a six-step chromatographic purification procedure, in which a prominent approximately 45-kDa band was found to copurify with processive polymerase activity. This species was identified as the product of the A20 gene. By use of recombinant viruses that direct the overexpression of A20 and/or the DNA polymerase, we verified the physical interaction between the two proteins in coimmunoprecipitation experiments. We also demonstrated that simultaneous overexpression of A20 and the DNA polymerase leads to a specific and robust increase in levels of processive polymerase activity. Taken together, we conclude that the A20 gene encodes a component of the processive DNA polymerase complex. Genetic data that further support this conclusion are presented in the accompanying report, which documents that temperature-sensitive mutants with lesions in the A20 gene have a DNA(-) phenotype that correlates with a deficit in processive polymerase activity (A. Punjabi et al, J. Virol. 75:12308-12318, 2001).
journal_name
J Viroljournal_title
Journal of virologyauthors
Klemperer N,McDonald W,Boyle K,Unger B,Traktman Pdoi
10.1128/JVI.75.24.12298-12307.2001subject
Has Abstractpub_date
2001-12-01 00:00:00pages
12298-307issue
24eissn
0022-538Xissn
1098-5514journal_volume
75pub_type
杂志文章abstract::The vast majority of studies with candidate immunogens based on the human immunodeficiency virus envelope (Env) have been conducted with Env proteins derived from clade B viruses isolated during chronic infection. Whether non-clade B Env protein immunogens will elicit antibodies with epitope specificities that are sim...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00389-08
更新日期:2008-06-01 00:00:00
abstract::The protein (p59rel) encoded by the transforming gene of reticuloendotheliosis virus strain T (REV-T) has been identified in REV-T-transformed avian lymphoid cells by using antisera raised against synthetic peptides whose sequences were derived from three nonoverlapping regions of v-rel (N. R. Rice, T. D. Copeland, S....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.59.1.120-126.1986
更新日期:1986-07-01 00:00:00
abstract::When ts7, a temperature-sensitive (ts) mutant of influenza B/Kanagawa/73 virus, infected MDCK cells at the nonpermissive temperature (37.5 degrees C), infectious virus was produced at very low levels compared with the yield at the permissive temperature (32 degrees C) and hemagglutinating activity and enzymatic activi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.1250-1254.1994
更新日期:1994-02-01 00:00:00
abstract::Rapid transmission of Borna disease virus occurred upon cohabitation of persistently infected and naive rats. Infectious virus, which was abundantly present in fresh urine samples of carrier rats, entered the brains of recipient rats via the olfactory route. Thus, susceptible farm animals possibly acquire the virus fr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.23.12886-12890.2003
更新日期:2003-12-01 00:00:00
abstract::The process of Epstein-Barr virus (EBV)-induced transformation of human B lymphocytes results in a cell line that is a mixture of latently and lytically infected cells, with the lytic cells composing roughly 5% to less than 0.0001% of the overall population. A set of nine normal lymphoblastoid cell lines that span a 1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.1.437-444.1990
更新日期:1990-01-01 00:00:00
abstract::In most human immunodeficiency virus type 1 (HIV-1)-infected individuals who achieve viral loads of <50 copies/ml during highly active antiretroviral therapy (HAART), low levels of plasma virus remain detectable for years by ultrasensitive methods. The relative contributions of ongoing virus replication and virus prod...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00570-09
更新日期:2009-10-01 00:00:00
abstract::Vectors derived from lentiviruses provide a promising gene delivery system. We examined the in vivo gene transfer efficiency and tissue or cell tropism of a feline immunodeficiency virus (FIV)-based lentiviral vector pseudotyped with the glycoproteins from Ross River Virus (RRV). RRV glycoproteins were efficiently inc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.18.9378-9388.2002
更新日期:2002-09-01 00:00:00
abstract::Human cytomegalovirus (HCMV) virions are composed of a DNA-containing nucleocapsid surrounded by a tegument layer and host-derived lipid envelope studded with virally encoded glycoproteins. These complex virions are estimated to be composed of more than 50 viral proteins. Assembly of HCMV virions is poorly understood,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01551-10
更新日期:2011-01-01 00:00:00
abstract::Mutations at amino acids 143, 148, and 155 in HIV-1 integrase (IN) define primary resistance pathways in subjects failing raltegravir (RAL)-containing treatments. Although each pathway appears to be genetically distinct, shifts in the predominant resistant virus population have been reported under continued drug press...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06618-11
更新日期:2012-07-01 00:00:00
abstract::Several viruses, including members of the gammaherpesvirus family, encode proteins that are secreted into the extracellular environment. We have identified an abundant 44-kDa secreted protein that is present in the supernatant of fibroblasts infected with murine gammaherpesvirus 68 (gammaHV68; also referred to as MHV-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.5.4524-4529.1999
更新日期:1999-05-01 00:00:00
abstract::After entering a host cell, retroviruses such as simian immunodeficiency virus (SIV) uncoat, disassembling the viral capsid. Rates of uncoating that are too high and too low can be detrimental to the efficiency of infection. Rapid uncoating typically leads to blocks in reverse transcription, but the basis for replicat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03239-12
更新日期:2013-04-01 00:00:00
abstract::We have characterized an inhibitory RNA element in the human immunodeficiency virus type 1 (HIV-1) gag coding sequence that prevents gag expression. The inhibition exerted by this element could be overcome by the presence of the Rev-responsive element in cis and of Rev protein in trans. To understand the mechanism of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.12.7176-7182.1992
更新日期:1992-12-01 00:00:00
abstract::Parvovirus capsids are small but complex molecular machines responsible for undertaking many of the steps of cell infection, genome packing, and cell-to-cell as well as host-to-host transfer. The details of parvovirus infection of cells are still not fully understood, but the processes must involve small changes in th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01871-16
更新日期:2017-01-03 00:00:00
abstract::West Nile virus (WNV) is a human pathogen that can cause neurological disorders, including meningoencephalitis. Experiments with mice and mammalian cell cultures revealed that WNV exhibited resistance to the innate immune program induced by alpha interferon (IFN-alpha). We have investigated the nature of this inhibiti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.3.1343-1350.2005
更新日期:2005-02-01 00:00:00
abstract::The matrix domain of human immunodeficiency virus type 1 Gag polyprotein was studied for its role in virus assembly. Deletion and substitution mutations caused a dramatic reduction in virus production. Mutant Gag polyproteins were myristoylated and had a high affinity for membrane association. Immunofluorescence stain...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.11.6387-6394.1993
更新日期:1993-11-01 00:00:00
abstract::In the immunocompetent host, primary cytomegalovirus (CMV) infection is resolved by the immune response without causing overt disease. The viral genome, however, is not cleared but is maintained in a latent state that entails a risk of virus recurrence and consequent organ disease. By using murine CMV as a model, we h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.1797-1804.1998
更新日期:1998-03-01 00:00:00
abstract::Human T-cell leukemia virus type 1 (HTLV-1) remains latent throughout the life of the carrier, with cells containing the provirus and viral gene expression efficiently down-regulated. On a molecular level, exactly how viruses are down-regulated in vivo remains unresolved. We described here the possibility that down-re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4485-4494.1997
更新日期:1997-06-01 00:00:00
abstract::When mouse L cells are infected for 22 hr with vesicular stomatitis virus (VSV), a ribonucleic acid-containing enveloped virus, greater than 70% of the major histocompatibility antigen (H-2), is no longer detectable by the method of inhibition of immune cytolysis. Infected cells prelabeled with (14)C-glucosamine also ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.4.578-585.1972
更新日期:1972-10-01 00:00:00
abstract::Guinea pig antisera and mouse monoclonal antibodies against a 40,000-molecular-weight nucleocapsid protein (p40) of herpes simplex virus types 1 and 2 immunoprecipitated 40,000- and 80,000-molecular-weight classes of soluble proteins from infected cell extracts. The soluble 40,000-molecular-weight protein class (intra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.3.644-652.1980
更新日期:1980-09-01 00:00:00
abstract:UNLABELLED:Human immunodeficiency virus type 1 (HIV-1) infection in the central nervous system (CNS) is characterized by replication in macrophages or brain microglia that express low levels of the CD4 receptor and is the cause of HIV-associated dementia and related cognitive and motor disorders that affect 20 to 30% o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01237-14
更新日期:2014-08-01 00:00:00
abstract::Nipah virus (NiV) and Hendra virus (HeV) are emerging zoonotic viruses and the causative agents of severe respiratory disease and encephalitis in humans. Little is known about the mechanisms that govern the development of respiratory and neurological disease. Using a hamster model of lethal NiV and HeV infection, we d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00473-11
更新日期:2011-08-01 00:00:00
abstract::Marburg virus, a filovirus, causes severe hemorrhagic fever with hitherto poorly understood molecular pathogenesis. We have investigated here the vectorial transport of the surface protein GP of Marburg virus in polarized epithelial cells. To this end, we established an MDCKII cell line that was able to express GP per...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.3.1274-1283.2001
更新日期:2001-02-01 00:00:00
abstract::A human lymphoid cell line (F172-D8) excreting a human immunodeficiency virus type 1 (HIV-1) anti-gp41 monoclonal antibody was used to construct a plasmid containing the cDNA of the single-chain variable fragment (scFvD8) corresponding to this antibody. A stable human osteosarcoma cell line was obtained which expresse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.12.5712-5715.2000
更新日期:2000-06-01 00:00:00
abstract::To dissect mechanisms of caprine arthritis-encephalitis lentivirus-induced arthritis, an undefined immunodominant viral glycoprotein, gp90 (G. C. Johnson, A. F. Barbet, P. Klevjer-Anderson, and T. C. McGuire, Infect. Immun. 41:657-665, 1983), was characterized. Monoclonal antibody to gp90 and specific antiserum to env...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.5.3247-3250.1992
更新日期:1992-05-01 00:00:00
abstract::Recently we showed that the membrane-proximal stem region of the vesicular stomatitis virus (VSV) G protein ectodomain (G stem [GS]), together with the transmembrane and cytoplasmic domains, was sufficient to mediate efficient VSV budding (C. S. Robison and M. A. Whitt, J. Virol. 74:2239-2246, 2000). Here, we show tha...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.23.12300-12311.2002
更新日期:2002-12-01 00:00:00
abstract::The human T-cell leukemia virus type 1 (HTLV-1) Tax protein activates the HTLV-1 long terminal repeat and key regulatory proteins involved in inflammation, activation, and proliferation and may induce cell transformation. Tax is also the immunodominant target antigen for cytotoxic T cells in HTLV-1 infection. We found...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.22.11106-11115.2001
更新日期:2001-11-01 00:00:00
abstract::Herpes simplex virus (HSV) virion host shutoff protein (vhs) destabilizes cellular and viral mRNAs. Previous work from several laboratories has indicated that vhs accelerates the turnover of most host mRNAs and provided evidence that at least some of these are degraded via endonucleolytic cleavage near regions of tran...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.7.4090-4098.2005
更新日期:2005-04-01 00:00:00
abstract::To assess the possible role of cytotoxic T lymphocytes (CTLs) in containing the spread of human immunodeficiency virus in acutely infected individuals, the temporal evolution of the virus-specific CD8+ lymphocyte response was defined in simian immunodeficiency virus of macaques (SIVmac)-infected rhesus monkeys. A brie...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1707-1711.1993
更新日期:1993-03-01 00:00:00
abstract::The ribonucleic acid (RNA) from Western equine encephalomyelitis (WEE) virions sedimented through sucrose gradients with a sedimentation coefficient of 40S. Another viral RNA which was always associated with infected cells possessed a sedimentation coefficient of 26S. Both 40S and 26S RNA had identical base compositio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.6.558-566.1968
更新日期:1968-06-01 00:00:00
abstract::Cytotoxic T lymphocytes (CTL) play an important role in recovery from a number of viral infections. They are also implicated in virus-induced immunopathology as best demonstrated in lymphocytic choriomeningitis virus (LCMV) infection of adult immunocompetent mice. In the present study, the structure of the T-cell rece...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.12.5919-5926.1990
更新日期:1990-12-01 00:00:00