Abstract:
:With the aid of three monoclonal antibodies (MAbs), a glycoprotein specifically localized to the outer envelope of vaccinia virus was shown to be encoded by the A33R gene. These MAbs reacted with a glycosylated protein that migrated as 23- to 28-kDa and 55-kDa species under reducing and nonreducing conditions, respectively. The protein recognized by the three MAbs was synthesized by all 11 orthopoxviruses tested: eight strains of vaccinia virus (including modified vaccinia virus Ankara) and one strain each of cowpox, rabbitpox, and ectromelia viruses. The observation that the protein synthesized by ectromelia virus-infected cells reacted with only one of the three MAbs provided a means of mapping the gene encoding the glycoprotein. By transfecting vaccinia virus DNA into cells infected with ectromelia virus and assaying for MAb reactivity, we mapped the glycoprotein to the A33R open reading frame. The amino acid sequence and hydrophilicity plot predicted that the A33R gene product is a type II membrane protein with two asparagine-linked glycosylation sites. Triton X-114 partitioning experiments indicated that the A33R gene product is an integral membrane protein. The ectromelia virus homolog of the vaccinia virus A33R gene was sequenced, revealing 90% predicted amino acid identity. The vaccinia and variola virus homolog sequences predict 94% identical amino acids, the latter having one fewer internal amino acid. Electron microscopy revealed that the A33R gene product is expressed on the surface of extracellular enveloped virions but not on the intracellular mature form of virus. The conservation of this protein and its specific incorporation into viral envelopes suggest that it is important for virus dissemination.
journal_name
J Viroljournal_title
Journal of virologyauthors
Roper RL,Payne LG,Moss Bdoi
10.1128/JVI.70.6.3753-3762.1996subject
Has Abstractpub_date
1996-06-01 00:00:00pages
3753-62issue
6eissn
0022-538Xissn
1098-5514journal_volume
70pub_type
杂志文章abstract::In order to study cell tropism and attenuation of hepatitis A virus (HAV), the genome of HAV wild-type GBM and two cell culture-adapted variants, GBM/FRhK and GBM/HFS, were cloned and sequenced after amplification by reverse transcriptase-PCR. During virus cultivation, the HAV variant GBM/FRhK had a strict host range ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.1.548-554.1994
更新日期:1994-01-01 00:00:00
abstract::The large scale of the Ebola virus disease (EVD) outbreak in West Africa in 2013-2016 raised the question whether the host cell interactions of the responsible Ebola virus (EBOV) strain differed from those of other ebolaviruses. We previously reported that the glycoprotein (GP) of the virus circulating in West Africa ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00177-17
更新日期:2017-04-13 00:00:00
abstract::The interaction of the human immunodeficiency virus type 1 (HIV-1) Pr55Gag molecule with the plasma membrane of an infected cell is an essential step of the viral life cycle. Myristic acid and positively charged residues within the N-terminal portion of MA constitute the membrane-binding domain of Pr55Gag. A separate ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.2723-2732.1998
更新日期:1998-04-01 00:00:00
abstract::Mus dunni endogenous virus (MDEV) is activated from cells of the Asian wild mouse M. dunni (also known as Mus terricolor) in response to treatment with either 5-iodo-2'-deoxyuridine or hydrocortisone. MDEV represents a new murine retrovirus interference group and thus appears to use a different receptor for entry into...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4663-4670.1997
更新日期:1997-06-01 00:00:00
abstract:UNLABELLED:The genus beta human papillomaviruses (beta HPVs) cause cutaneous lesions and are thought to be involved in the initiation of some nonmelanoma skin cancers (NMSCs), particularly in patients with the genetic disorder epidermodysplasia verruciformis (EV). We have previously reported that at least two of the ge...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01197-14
更新日期:2014-08-01 00:00:00
abstract::Human influenza viruses are more efficiently isolated by inoculating patient samples into the amniotic rather than the allantoic cavity of embryonated chicken eggs. This type of cultivation selects virus variants with mutations around the hemagglutinin (HA) receptor binding site. To understand the molecular basis of t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.4.3357-3362.1997
更新日期:1997-04-01 00:00:00
abstract::One hundred thirty-eight new Epstein-Barr virus (EBV) genome sequences have been determined. One hundred twenty-five of these and 116 from previous reports were combined to produce a multiple-sequence alignment of 241 EBV genomes, which we have used to analyze variation within the viral genome. The type 1/type 2 class...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01132-18
更新日期:2018-10-29 00:00:00
abstract:UNLABELLED:A balance between the functions of the influenza virus surface proteins hemagglutinin (HA) and neuraminidase (NA) is thought to be important for the transmission of viruses between humans. Here we describe two pandemic H1N1 viruses, A/swine/Virginia/1814-1/2012 and A/swine/Virginia/1814-2/2012 (pH1N1low-1 an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02537-14
更新日期:2015-06-01 00:00:00
abstract::Vaccinia virus DNA fragments that have been denatured by alkali and then neutralized contain a fraction that rapidly reforms duplex structures. The fraction is enriched by fractionating on hydroxyapatite columns and serves as as substrate for digestion by two restriction endonucleases isolated from Hemophilus parainfl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.1.227-238.1976
更新日期:1975-01-01 00:00:00
abstract::In some retroviruses, such as Rous sarcoma virus and prototype foamy virus, Gag proteins are known to shuttle between the nucleus and the cytoplasm and are implicated in nuclear export of the viral genomic unspliced RNA (gRNA) for subsequent encapsidation. A similar function has been proposed for human immunodeficienc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06874-11
更新日期:2012-03-01 00:00:00
abstract::3-O-(3',3'-dimethylsuccinyl)betulinic acid (PA-457 or bevirimat) potently inhibits human immunodeficiency virus type 1 (HIV-1) maturation by blocking a late step in the Gag processing pathway, specifically the cleavage of SP1 from the C terminus of capsid (CA). To gain insights into the mechanism(s) by which HIV-1 cou...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01369-06
更新日期:2006-11-01 00:00:00
abstract::We have constructed a series of vectors based on simian foamy virus type 1 (SFV-1) to define the minimum cis-acting elements required for gene transfer. To characterize these vectors, we inserted the coding sequence of the bacterial lacZ gene linked to the cytomegalovirus immediate-early gene promoter. Introduction of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3451-3454.1998
更新日期:1998-04-01 00:00:00
abstract::To study the variability of human immunodeficiency virus type 1 (HIV-1), we used immunotoxins to select for variants within a population of H9 cells persistently infected with a molecular clone of HIV-1 designated NL4-3. Chimeric immunotoxin CD4-PE40 (a chimeric fusion protein consisting of the amino-terminal two doma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.75-81.1995
更新日期:1995-01-01 00:00:00
abstract::Cultural changes that follow infection of rabbit kidney cells with fibroma virus were studied. Characteristic alterations of cell morphology and development of multilayered piles and cords of cells were found to occur in infected cultures in which cell division was blocked by gamma radiation or by cell crowding and se...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.5.603-609.1969
更新日期:1969-11-01 00:00:00
abstract::A porcine rotavirus (prv), variant 4F, isolated in tissue culture from the feces of a Chinese pig with diarrhea, was found to have become highly pathogenic when passaged in gnotobiotic piglets (J. C. Bridger, B. Burke, G. M. Beards, and U. Desselberger, J. Gen. Virol. 73:3011-3015, 1992). Comparison with the closely r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.853-857.1997
更新日期:1997-01-01 00:00:00
abstract::The polypeptide and glycoprotein compositions of the mouse mammary tumor virus virion from primary monolayer cultures of BALB/cfC3H mouse mammary tumor cells were studied by polyacrylamide gel electrophoresis by using internal and external labeling and Coomassie blue and periodic acid Schiff (PAS) staining. Twelve pol...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.2.411-418.1974
更新日期:1974-02-01 00:00:00
abstract::Recombinant DNA clones of a viral clone of spleen necrosis virus, an avian retrovirus, were found to have long terminal repeats of different sizes. The variation was in the U3 region of the long terminal repeats, and any one clone had U3 of the same size in both long terminal repeats. The U3 regions in the 5' and 3' l...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.1.163-171.1982
更新日期:1982-01-01 00:00:00
abstract::Identification of cell surface viral binding proteins is important for understanding viral attachment and internalization. We have fused the pre-S domain of the duck hepatitis B virus (DHBV) large envelope protein to glutathione S-transferase and demonstrated a 170-kDa binding protein (p170) in [35S]methionine-labeled...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.7106-7112.1995
更新日期:1995-11-01 00:00:00
abstract::APOBEC3G (A3G) is a cytidine deaminase that restricts human immunodeficiency virus type 1 (HIV-1) and other lentiviruses. Most of these viruses encode a Vif protein that directly binds A3G and leads to its proteasomal degradation. Both Vif proteins of HIV-1 and African green monkey simian immunodeficiency virus (SIVag...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01944-13
更新日期:2013-11-01 00:00:00
abstract::A vaccinia-directed deoxyribonucleic acid (DNA) polymerase has been partially purified from the cytoplasmic fractions of virus-infected HeLa cells. The utilization of natural and synthetic templates by this enzyme resembles that of the host cell DNA-dependent DNA polymerases. The vaccinia DNA polymerase cannot copy ri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.4.721-729.1972
更新日期:1972-10-01 00:00:00
abstract::Novel molecular approaches to inhibit human immunodeficiency virus type 1 (HIV-1) infection have received increasing attention because of the lack of effective antiviral drug therapies in vivo. We now demonstrate that cells can be intracellularly immunized by cytoplasmic expression of single-chain variable antibody fr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.3392-3400.1996
更新日期:1996-06-01 00:00:00
abstract::In this study, we characterized the viral determinants of coreceptor usage in relation to susceptibility to antibody-mediated neutralization or enhancement of infectivity by using chimeras of three highly related human immunodeficiency virus type 1 (HIV-1) isolates of different phenotypes. We found that the V3 region ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.6.2827-2834.2002
更新日期:2002-03-01 00:00:00
abstract::The amphotropic murine leukemia virus (MuLV) can infect cells from a number of mammals, including humans, via its specific receptor. Basic knowledge of amphotropic MuLV receptor expression is likely to be useful in the development and improvement of gene therapy protocols based on amphotropic-pseudotyped vectors. To i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4564-4570.1997
更新日期:1997-06-01 00:00:00
abstract::The retinoblastoma tumor suppressor protein (pRb) can associate with the transforming proteins of several DNA tumor viruses, including the large T antigen encoded by polyomavirus (Py T Ag). Although pRb function is critical for regulating progression from G1 to S phase, a role for pRb in S phase has not been demonstra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.3004-3013.1999
更新日期:1999-04-01 00:00:00
abstract::Latency is an integral feature of the pathogenesis of cytomegalovirus infection and disease. Using in situ hybridization, we detected viral DNA in the splenic stroma of mice with acute murine cytomegalovirus (MCMV) infection but could not detect latent infection. By using enzymatic amplification of a 700-bp region of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.6.3330-3334.1991
更新日期:1991-06-01 00:00:00
abstract::A tetracycline-regulated gene expression system and a panel of novel monoclonal antibodies were used to examine the subcellular localization, stability, and trans-cleavage competence of the hepatitis C virus (HCV) NS3-NS4A complex in inducible cell lines. The NS3 serine protease domain and the full-length NS3 protein ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.5.2293-2304.2000
更新日期:2000-03-01 00:00:00
abstract::Structural proteins of temperature-sensitive (ts) mutants of vesicular stomatitis virus, Indiana serotype, were compared with those of wild-type and revertant virions by electrophoresis on polyacrylamide gels of partial digests with Staphylococcus aureus V8 protease. Mutants of complementation groups III (tsG31 and ts...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.1.248-255.1981
更新日期:1981-01-01 00:00:00
abstract::The viral genes UL128, UL130, and UL131A have been identified as major determinants of endothelial cell (EC) tropism of human cytomegalovirus (HCMV), with deletion of either gene causing a null phenotype. We hypothesized that a functional scanning of these genes by minor genetic modifications would allow for the gener...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01069-08
更新日期:2008-11-01 00:00:00
abstract::The global diversity of human immunodeficiency virus type 1 (HIV-1) genotypes, termed subtypes A to J, is considerable and growing. However, relatively few studies have provided evidence for an associated phenotypic divergence. Recently, we demonstrated subtype-specific functional differences within the long terminal ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.10.8446-8452.1998
更新日期:1998-10-01 00:00:00
abstract::Infection of macaque monkeys with simian immunodeficiency virus (SIV) is probably the best animal model currently available for studying acquired immunodeficiency syndrome. In this report, we describe three infectious molecular clones of SIVmac and one of human immunodeficiency virus type 2 (HIV-2) and their use in th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.12.4691-4696.1988
更新日期:1988-12-01 00:00:00