Abstract:
:Procedures for characterizing replication-defective viruses in nonpermissive mammalian cells were developed and applied to three nonvirogenic Rous sarcoma virus (RSV)-transformed mammalian cell lines--B4, a line of Bryan virus-transformed hamster cells, and two SRD-RSV transformed rat cell lines, LR3/1 and LR3/2. Cell fusion was used to study virus complementation. The three cell lines (i) fused with helper virus-infected chicken cells and the host range of the rescued virus examined, (ii) tested for complementation by fusion with chicken cells exhibiting various patterns of endogenous virus expression, (iii) fused with chicken cells infected with the temperature-sensitive replication mutant LA334 and assayed for complementation at permissive and nonpermissive temperatures, and (iv) tested for complementation of defective viruses in other RSV-transformed mammalian cell lines by fusing pairs of nonvirogenic cell lines and permissive chicken cells. Based upon these complementation studies, we concluded that B4 virus is defective only in the env gene, LR3/) virus is an absolute mutant in the gag and/or pol genes, and LR3/2 virus is a leaky env mutant. Clones of LR3/1 and LR3/2 virus-infected chicken cells were established, and the results obtained from the characterization of these viruses in permissive avian cells substantiates the conclusions reached in the fusion-rescue studies.
journal_name
J Viroljournal_title
Journal of virologyauthors
Steimer KS,Boettiger Ddoi
10.1128/JVI.32.1.175-186.1979subject
Has Abstractpub_date
1979-10-01 00:00:00pages
175-86issue
1eissn
0022-538Xissn
1098-5514journal_volume
32pub_type
杂志文章abstract::The expression and predicted products of rat c-ros-1 gene, the proto-oncogene of v-ros in UR2 sarcoma virus, were characterized. The c-ros-1 gene was found to be expressed in a tissue-specific manner, and the sizes of its transcripts were heterogeneous: 8.2 kilobases (kb) long in lung and kidney tissues, 6.9 kb in hea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2117-2125.1990
更新日期:1990-05-01 00:00:00
abstract::Interallelic complementation between certain temperature-sensitive mutants of gene 42 of bacteriophage T4 was demonstrated by measuring the incorporation of labeled thymine into DNA. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.2.415-416.1973
更新日期:1973-08-01 00:00:00
abstract::Retroelements are important evolutionary forces but can be deleterious if left uncontrolled. Members of the human APOBEC3 family of cytidine deaminases can inhibit a wide range of endogenous, as well as exogenous, retroelements. These enzymes are structurally organized in one or two domains comprising a zinc-coordinat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01491-09
更新日期:2009-12-01 00:00:00
abstract::A ribonucleic acid (RNA)-dependent RNA polymerase was induced in chick embryo fibroblast cells after infection with Sendai virus (parainfluenza 1 virus). The enzyme was associated with the microsomal fraction of infected cells and reached maximum detectable activity at 18 hr after virus infection. The activity of the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.6.663-671.1970
更新日期:1970-06-01 00:00:00
abstract::Marburg virus (MARV) has been associated with sporadic episodes of hemorrhagic fever, including a recent highly publicized outbreak in Angola that produced severe disease and significant mortality in infected patients. MARV is also considered to have potential as a biological weapon. Recently, we reported the developm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00959-06
更新日期:2006-10-01 00:00:00
abstract::Autophagy is an intracellular degradation pathway that provides a host defense mechanism against intracellular pathogens. However, many viruses exploit this mechanism to promote their replication. This study shows that lytic induction of Epstein-Barr virus (EBV) increases the membrane-bound form of LC3 (LC3-II) and LC...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02033-14
更新日期:2014-10-01 00:00:00
abstract::During EBV infection, lytic DNA replication activates late gene expression in trans via an uncharacterized pathway. In this study, we mapped the target of this regulatory cascade to a variant TATA box (TATTAAA) and the 3' flanking region within the core promoter of the BcLF1 gene. The inherent late activity of this co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.10.8338-8343.1998
更新日期:1998-10-01 00:00:00
abstract::The 70S RNA of Rous sarcoma virus contains 4S RNAs which serve as primers for the initiation of DNA synthesis in vitro by the RNA-directed DNA polymerase of the virus. We purified these primers in three different ways-by isolation of the covalent complex between primer and nascent DNA, by differential melting of the 7...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.5.1134-1142.1974
更新日期:1974-05-01 00:00:00
abstract::Polyomavirus large T antigen has an N-terminal domain of approximately 260 amino acids which can immortalize primary cells but lacks sequences known to be required for DNA binding and replication. Treatment of full-length large T with either V8 protease or chymotrypsin yields an N-terminal fragment of 36 to 40 kDa and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.668-673.1994
更新日期:1994-02-01 00:00:00
abstract::We have identified a spliced transcript that contains sequences from the HCMV UL29 and UL28 open reading frames. It contains amino-terminal UL29 sequences followed by UL28 sequences, and it includes a poly(A) signal derived from the 3'-untranslated region following the UL26 open reading frame. UL29/28 RNA is expressed...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00396-09
更新日期:2009-10-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) readily infects both humans and chimpanzees, but the pathologic outcomes of infection in these two species differ greatly. In attempts to identify virus-cell interactions that might account for this differential pathogenicity, chimpanzee peripheral blood lymphocytes and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.6.3344-3348.1991
更新日期:1991-06-01 00:00:00
abstract:UNLABELLED:Foot-and-mouth disease virus (FMDV) causes a highly contagious, debilitating disease in cloven-hoofed animals with devastating economic consequences. To survive in the host, FMDV has evolved to antagonize the host type I interferon (IFN) response. Previous studies have reported that the leader proteinase (L(...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03668-13
更新日期:2014-05-01 00:00:00
abstract::Transfection of an Epstein-Barr virus (EBV)-encoded plasmid containing EBER caused a substantial decrease in the level of plasmid containing EBV in Akata and Mutu Burkitt's lymphoma (BL) lines, but failed to do so in other BL lines. The results suggest that EBER could replace the role of EBV, but other EBV products al...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.20.9977-9982.2001
更新日期:2001-10-01 00:00:00
abstract::The internal structural proteins of retroviruses are proteolytically processed from the Gag polyprotein, which alone is able to assemble into virus-like particles when expressed in cells. All Gag proteins contain domains corresponding to the three structural proteins MA, CA, and NC. We have expressed the CA and NC dom...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.10.6487-6497.1995
更新日期:1995-10-01 00:00:00
abstract::Highly conserved amino acids in the second helix structure of the human immunodeficiency virus type 1 (HIV-1) MA protein were identified to be critical for the incorporation of viral Env proteins into HIV-1 virions from transfected COS-7 cells. The effects of these MA mutations on viral replication in the HIV-1 natura...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.1443-1452.1997
更新日期:1997-02-01 00:00:00
abstract::HVEM (for herpesvirus entry mediator) is a member of the tumor necrosis factor receptor superfamily and mediates entry of many strains of herpes simplex virus (HSV) into normally nonpermissive Chinese hamster ovary (CHO) cells. We used sucrose density centrifugation to demonstrate that purified HSV-1 KOS virions bind ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.3595-3601.1998
更新日期:1998-05-01 00:00:00
abstract::We have used a V3-specific heteroduplex tracking assay (V3-HTA) with probes from two different human immunodeficiency virus type 1 (HIV-1) subtypes to examine the extent and pace of HIV-1 evolution late in infection. Twenty-four subjects with advanced HIV-1 infection (CD4(+) T-cell count, <100/microl) and stable viral...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.18.8494-8501.2000
更新日期:2000-09-01 00:00:00
abstract::Cowpox virus (CPXV) belongs to the genus Orthopoxvirus in the Poxviridae family. It infects a broad range of vertebrates and can cause zoonotic infections. CPXV has the largest genome among the orthopoxviruses and is therefore considered to have the most complete set of genes of all members of the genus. Since CPXV ha...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02385-13
更新日期:2014-01-01 00:00:00
abstract::The nonstructural protein (NSs) of severe fever with thrombocytopenia syndrome phlebovirus (SFTSV) sequesters TANK-binding kinase 1 (TBK1) into NSs-induced cytoplasmic structures to inhibit the phosphorylation and nuclear translocation of interferon (IFN) regulatory factor 3 (IRF3) and subsequent interferon beta (IFN-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00706-18
更新日期:2018-09-12 00:00:00
abstract::The route of internalization of the pestivirus bovine viral diarrhea virus (BVDV) was studied by using different chemical and biophysical inhibitors of endocytosis. Expression of the dominant-negative mutant Dyn(K44A) of the GTPase dynamin in MDBK cells, as well as the treatment of the cells with chlorpromazine and be...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.7.4191-4200.2005
更新日期:2005-04-01 00:00:00
abstract:UNLABELLED:Influenza A virus requires ongoing cellular transcription to carry out the cap-snatching process. Chromatin remodelers modify chromatin structure to produce an active or inactive conformation, which enables or prevents the recruitment of transcriptional complexes to specific genes; viral transcription thus d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00053-16
更新日期:2016-01-20 00:00:00
abstract::We have developed a reconstituted system which models the events associated with human immunodeficiency virus type 1 (HIV-1) plus-strand transfer. These events include synthesis of plus-strand strong-stop DNA [(+) SSDNA] from a minus-strand DNA donor template covalently attached to human tRNA3Lys, tRNA primer removal,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.6.4794-4805.1999
更新日期:1999-06-01 00:00:00
abstract::Cell surface receptors exploited by human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) for infection are major determinants of tropism. HIV-1 usually requires two receptors to infect cells. Gp120 on HIV-1 virions binds CD4 on the cell surface, triggering conformational rearrangements that creat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.9.7795-7804.1999
更新日期:1999-09-01 00:00:00
abstract::Hepatitis delta virus (HDV), a single-stranded RNA virus, bears a single coding region whose product, the hepatitis delta antigen (HDAg), is expressed in two isoforms, small (S-HDAg) and large (L-HDAg). S-HDAg is required for replication of HDV, while L-HDAg inhibits viral replication and is required for the envelopme...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.11.5329-5336.2000
更新日期:2000-06-01 00:00:00
abstract::Human cytomegalovirus (HCMV) has evolved multiple strategies for suppression of the antiviral response of the infected cell. DNA array technology has revealed that HCMV clearly regulates host gene expression during the course of a productive infection by enhancing, sustaining, or suppressing steady-state levels of cel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.1.472-485.2005
更新日期:2005-01-01 00:00:00
abstract::Satellite RNAs are the smallest infectious agents whose replication is thought to be completely dependent on their helper virus (HV). Here we report that, when expressed autonomously in the absence of HV, a variant of satellite RNA (satRNA) associated with Cucumber mosaic virus strain Q (Q-satRNA) has a propensity to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00018-12
更新日期:2012-05-01 00:00:00
abstract::Viral interference studies have demonstrated the existence of four distinct murine leukemia virus (MuLV) receptors on NIH 3T3 mouse cells. The four viral interference groups are ecotropic MuLV; mink cell focus inducing virus (MCF); amphotropic MuLV; and 10A1, a recombinant derivative of amphotropic MuLV that uses a un...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.2.757-766.1990
更新日期:1990-02-01 00:00:00
abstract::Naked DNA vaccines expressing the prM and E genes of two tick-borne flaviviruses, Russian spring summer encephalitis (RSSE) virus and Central European encephalitis (CEE) virus were evaluated in mice. The vaccines were administered by particle bombardment of DNA-coated gold beads by Accell gene gun inoculation. Two imm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.12.9563-9569.1997
更新日期:1997-12-01 00:00:00
abstract::Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus that has a worldwide distribution in the population of cattle. Many factors make human contamination by BoHV-4 likely to occur. In this study, we performed in vitro experiments to assess the risk and the consequences of human infection by BoHV-4. First, by using a re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.5.2336-2347.2004
更新日期:2004-03-01 00:00:00
abstract::The rate of transcription in isolated nuclei of primary mouse kidney cells increases by a factor of about 1.5 between 5 and 8 h after infection with polyoma virus or simian virus 40. This process requires intact virus and is inhibited by cycloheximide and actinomycin D. It appears to involve the activity of all three ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.1.8-19.1980
更新日期:1980-07-01 00:00:00