Abstract:
:The chimeric murine oncornavirus FrCas(E) causes a rapidly progressive paralytic disease associated with spongiform neurodegeneration throughout the neuroaxis. Neurovirulence is determined by the sequence of the viral envelope gene and by the capacity of the virus to infect microglia. The neurocytopathic effect of this virus appears to be indirect, since the cells which degenerate are not infected. In the present study we have examined the possible role of inflammatory responses in this disease and have used as a control the virus F43. F43 is an highly neuroinvasive but avirulent virus which differs from FrCas(E) only in 3' pol and env sequences. Like FrCas(E), F43 infects large numbers of microglial cells, but it does not induce spongiform neurodegeneration. RNAase protection assays were used to detect differential expression of genes encoding a variety of cytokines, chemokines, and inflammatory cell-specific markers. Tumor necrosis factor alpha (TNF-alpha) and TNF-beta mRNAs were upregulated in advanced stages of disease but not early, even in regions with prominent spongiosis. Surprisingly there was no evidence for upregulation of the cytokines interleukin-1 alpha (IL-1 alpha), IL-1 beta, and IL-6 or of the microglial marker F4/80 at any stage of this disease. In contrast, increased levels of the beta-chemokines MIP-1 alpha and -beta were seen early in the disease and were concentrated in regions of the brain rich in spongiosis, and the magnitude of responses was similar to that observed in the brains of mice injected with the glutamatergic neurotoxin ibotenic acid. MIP-1alpha and MIP-1beta mRNAs were also upregulated in F43-inoculated mice, but the responses were three- to fivefold lower and occurred later in the course of infection than was observed in FrCas(E)-inoculated mice. These results suggest that the robust increase in expression of MIP-1 alpha and MIP-1 beta in the brain represents a correlate of neurovirulence in this disease, whereas the TNF responses are likely secondary events.
journal_name
J Viroljournal_title
Journal of virologyauthors
Askovic S,Favara C,McAtee FJ,Portis JLdoi
10.1128/JVI.75.6.2665-2674.2001subject
Has Abstractpub_date
2001-03-01 00:00:00pages
2665-74issue
6eissn
0022-538Xissn
1098-5514journal_volume
75pub_type
杂志文章abstract::Borna disease virus (BDV) is a nonsegmented negative-strand (NNS) RNA virus that is unusual because it replicates in the nucleus. The most abundant viral protein in infected cells is a 38/39-kDa doublet that is presumed to represent the nucleocapsid. Infectious particles also contain high levels of this protein, accou...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5133-5139.1997
更新日期:1997-07-01 00:00:00
abstract::To investigate the genetic stability of vaccinia virus DNA, we have tested whether alterations occurred in the polypeptide composition of this complex virus during persistent infections. We found that variants isolated at various passages in Friend erythroleukemia cells persistently infected with vaccinia virus contai...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.8.2642-2647.1987
更新日期:1987-08-01 00:00:00
abstract:UNLABELLED:Woodchuck hepatitis virus (WHV), a close relative of human hepatitis B virus (HBV), has been a key model for disease progression and clinical studies. Sequences of the assembly domain of WHV and HBV core proteins (wCp149 and hCp149, respectively) have 65% identity, suggesting similar assembly behaviors. We r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01840-14
更新日期:2014-12-01 00:00:00
abstract::The gB glycoprotein of herpes simplex virus type 1 is involved in viral entry and fusion and contains a predicted membrane-anchoring sequence of 69 hydrophobic amino acids, which can span the membrane three times, near the carboxy terminus. To define the membrane-anchoring sequence and the role of this hydrophobic str...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.4856-4866.1993
更新日期:1993-08-01 00:00:00
abstract::The process of Epstein-Barr virus (EBV)-induced transformation of human B lymphocytes results in a cell line that is a mixture of latently and lytically infected cells, with the lytic cells composing roughly 5% to less than 0.0001% of the overall population. A set of nine normal lymphoblastoid cell lines that span a 1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.1.437-444.1990
更新日期:1990-01-01 00:00:00
abstract::The human papillomavirus type 11 (HPV-11) L1 major capsid protein can be trypsinized to generate recombinant capsomeres that retain HPV genotype-restricted capsid antigenicity (M. Li, T. P. Cripe, P. A. Estes, M. K. Lyon, R. C. Rose, and R. L. Garcea, J. Virol. 71:2988-2995, 1997). In the present study, HPV-11 virion-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.6151-6154.1998
更新日期:1998-07-01 00:00:00
abstract::The presence of a high number of activated T cells in the bloodstream and spontaneous proliferation of peripheral blood mononuclear cells in vitro are striking characteristics of human T-cell leukemia virus type I (HTLV-I) infection. The HTLV-I regulatory protein Tax and the envelope protein gp46 have been implicated ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.6.3134-3141.1993
更新日期:1993-06-01 00:00:00
abstract::Minute virus of canines (MVC) is a member of the genus Bocavirus in the family Parvoviridae. We have molecularly cloned and sequenced the 5'- and 3'-terminal palindromes of MVC. The MVC genome, 5,404 nucleotides (nt) in length, shared an identity of 52.6% and 52.1% with that of human bocavirus and bovine parvovirus, r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02569-08
更新日期:2009-04-01 00:00:00
abstract::The transmembrane fusion (F) glycoprotein of measles virus is an important target antigen of human HLA class I- and class II-restricted cytotoxic T lymphocytes (CTL). Genetically engineered F proteins and nested sets of synthetic peptides spanning the F protein were used to determine sequences of F recognized by a num...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.2276-2284.1993
更新日期:1993-04-01 00:00:00
abstract::Two foot-and-mouth disease virus (FMDV) genome sequences have been determined for isolates collected from recent field outbreaks in North Africa (Egypt) and the Middle East (Palestinian Autonomous Territories). These data represent the first examples of complete genomic sequences for the FMDV SAT 2 topotype VII, which...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01231-12
更新日期:2012-08-01 00:00:00
abstract::A virus (M-7) isolated from baboon placental tissue demonstrates many similarities to endogenous feline virus RD-114. Immunodiffusion analysis shows a group-specific antigen (gs-1) line of identity between M-7 and RD-114. Anti-RD-114 DNA polymerase IgG inhibits M-7 polymerase by 57% compared to 97% for RD-114. M-7 vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.1.133-138.1974
更新日期:1974-07-01 00:00:00
abstract::In the influenza H5N1 virus incident in Hong Kong in 1997, viruses that are closely related to H5N1 viruses initially isolated in a severe outbreak of avian influenza in chickens were isolated from humans, signaling the possibility of an incipient pandemic. However, it was not possible to prepare a vaccine against the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8303-8307.1999
更新日期:1999-10-01 00:00:00
abstract::The preintegration complex of human immunodeficiency virus type 1 (HIV-1) is a large nucleoprotein complex containing viral nucleic acids in association with products of the viral gag and pol genes. One of these proteins, integrase, is absolutely required for the integration and formation of the provirus. Although HIV...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.11.6863-6865.1993
更新日期:1993-11-01 00:00:00
abstract::Foamy viruses (FVs) are the least known retroviruses commonly found in primates, cats, horses, and cattle. Although FVs are considered apathogenic, simian and feline FVs have been shown to be associated with some transient health abnormalities in animal models. Currently, data regarding the course of infection with bo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02447-12
更新日期:2013-03-01 00:00:00
abstract::Cleavage of varicella-zoster virus DNA with the restriction endonucleases PstI, XbaI, and BglII resulted in 18, 22, and 20 fragments, respectively. Based on the molecular weights and molarities of these fragments, a molecular weight of 84 x 10(6) could be calculated for the varicella-zoster virus genome. In both the X...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.2.390-400.1981
更新日期:1981-08-01 00:00:00
abstract::RNA recombination is a major process in promoting rapid virus evolution in an infected host. A previous genome-wide screen with the yeast single-gene deletion library of 4,848 strains, representing approximately 80% of all genes of yeast, led to the identification of 11 host genes affecting RNA recombination in Tomato...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.3.1231-1241.2006
更新日期:2006-02-01 00:00:00
abstract::Respiratory syncytial virus (RSV) is a major cause of morbidity and mortality. Previous studies have suggested that T-cell responses may contribute to RSV immunopathology, which could be driven by dendritic cells (DCs). DCs are productively infected by RSV, and during RSV infections, there is an increase of DCs in the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01273-10
更新日期:2010-12-01 00:00:00
abstract::Prophylactic hepatitis C virus (HCV) vaccine trials with human volunteers are pending. There is an important need for immunological end points which correlate with vaccine efficacy and which do not involve invasive procedures, such as liver biopsies. By using a multicomponent DNA priming-protein boosting vaccine strat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.1.187-196.2004
更新日期:2004-01-01 00:00:00
abstract::We had previously demonstrated that a cellular protein specifically interacts with the 3' end of poliovirus negative-strand RNA. We now report the identity of this protein as heterogeneous nuclear ribonucleoprotein (hnRNP) C1/C2. Formation of an RNP complex with poliovirus RNA was severely impaired by substitution of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.6.3254-3266.2005
更新日期:2005-03-01 00:00:00
abstract::Although there is increasing evidence that individuals already infected with human immunodeficiency virus type 1 (HIV-1) can be infected with a heterologous strain of the virus, the extent of protection against superinfection conferred by the first infection and the biologic consequences of superinfection are not well...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02237-08
更新日期:2009-03-01 00:00:00
abstract::The current therapy for hepatitis B and C is based on systemic administration of recombinant human alpha interferon (r-hIFN-alpha). However, systemic delivery of r-hIFN-alpha is associated with severe side effects, but more importantly, it is effective in only a small percentage of patients. In an effort to maximize I...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.10.4816-4823.2000
更新日期:2000-05-01 00:00:00
abstract::Mouse-hamster somatic cell hybrids were used to show that the recombinant mink cell focus-forming murine leukemia viruses and their ecotropic virus progenitors require different mouse chromosomes for replication. Mouse chromosome 1 was shown to carry the genetic information necessary for the replication of six differe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.48.1.300-303.1983
更新日期:1983-10-01 00:00:00
abstract:UNLABELLED:Bean pod mottle virus (BPMV) is a bipartite, positive-sense (+) RNA plant virus in the Secoviridae family. Its RNA1 encodes proteins required for genome replication, whereas RNA2 primarily encodes proteins needed for virion assembly and cell-to-cell movement. However, the function of a 58-kDa protein (P58) e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03301-13
更新日期:2014-03-01 00:00:00
abstract::Interspecies transmission of the transmissible spongiform encephalopathies (TSEs), or prion diseases, can result in the adaptation and selection of TSE strains with an expanded host range and increased virulence such as in the case of bovine spongiform encephalopathy and variant Creutzfeldt-Jakob disease. To investiga...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.12.5542-5547.2000
更新日期:2000-06-01 00:00:00
abstract::To determine mechanisms by which persistent viral infections are established and maintained, we initiated persistent infections of murine erythroleukemia (MEL) cells by using reovirus strains type 3 Abney and type 3 Dearing. Establishment of persistent reovirus infections of MEL cells was not associated with a signifi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.299-306.1997
更新日期:1997-01-01 00:00:00
abstract::During infection, adenovirus-associated virus (AAV) undergoes microtubule-dependent retrograde transport as part of a program of vectorial transport of viral genome to the nucleus. A microtubule binding assay was used to evaluate the hypothesis that cytoplasmic dynein mediates AAV interaction with microtubules. Bindin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00481-06
更新日期:2006-08-01 00:00:00
abstract:UNLABELLED:Baculovirus DNAs are synthesized and inserted into preformed capsids to form nucleocapsids at a site in the infected cell nucleus, termed the virogenic stroma. Nucleocapsid assembly ofAutographa californicamultiple nucleopolyhedrovirus (AcMNPV) requires the major capsid protein VP39 and nine minor capsid pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02885-15
更新日期:2016-03-28 00:00:00
abstract::Alkylation of T7 bacteriophage by methyl methane sulfonate blocked superinfection exclusion. This blockage could be correlated with a delay in the synthesis of phage-specific proteins. Therefore we conclude that protein synthesis directed by the primary infecting phage is required for efficient exclusion of superinfec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.2.708-710.1982
更新日期:1982-11-01 00:00:00
abstract::A retrovirus endogenous to guinea pig cells was earlier shown to be morphologically similar to type B and type D prototype retroviruses. Molecular hybridization techniques were used to show that guinea pig virus nucleotide sequences are endogenous to both domestic (Cavia porcellus) and indigenous (Cavia aperea) guinea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.1.522-530.1980
更新日期:1980-01-01 00:00:00
abstract::Bluetongue virus (BTV), a member of the Reoviridae family, is an insect-borne animal pathogen. Virus release from infected cells is predominantly by cell lysis, but some BTV particles are also released from the plasma membrane. The nonstructural protein NS3 has been implicated in this process. Using alternate initiato...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02352-10
更新日期:2011-05-01 00:00:00