Abstract:
:The majority of people infected with hepatitis C virus (HCV) fail to generate or maintain a T-cell response effective for viral clearance. Evidence from murine chronic viral infections shows that expression of the coinhibitory molecule PD-1 predicts CD8+ antiviral T-cell exhaustion and may contribute to inadequate pathogen control. To investigate whether human CD8+ T cells express PD-1 and demonstrate a dysfunctional phenotype during chronic HCV infection, peripheral and intrahepatic HCV-specific CD8+ T cells were examined. We found that in chronic HCV infection, peripheral HCV-specific T cells express high levels of PD-1 and that blockade of the PD-1/PD-L1 interaction led to an enhanced proliferative capacity. Importantly, intrahepatic HCV-specific T cells, in contrast to those in the periphery, express not only high levels of PD-1 but also decreased interleukin-7 receptor alpha (CD127), an exhausted phenotype that was HCV antigen specific and compartmentalized to the liver, the site of viral replication.
journal_name
J Viroljournal_title
Journal of virologyauthors
Radziewicz H,Ibegbu CC,Fernandez ML,Workowski KA,Obideen K,Wehbi M,Hanson HL,Steinberg JP,Masopust D,Wherry EJ,Altman JD,Rouse BT,Freeman GJ,Ahmed R,Grakoui Adoi
10.1128/JVI.02021-06subject
Has Abstractpub_date
2007-03-01 00:00:00pages
2545-53issue
6eissn
0022-538Xissn
1098-5514pii
JVI.02021-06journal_volume
81pub_type
杂志文章abstract::Cytotoxic T lymphocytes (CTL) recognize virus peptide fragments complexed with class I major histocompatibility complex (MHC) molecules on the surface of virus-infected cells. Recognition is mediated by a membrane-bound T-cell receptor (TCR) composed of alpha and beta chains. Studies of the CTL response to lymphocytic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.4.2527-2531.1992
更新日期:1992-04-01 00:00:00
abstract::The highly syncytium-inducing M strain and the weakly syncytium-inducing SC strain of bovine parainfluenza 3 virus differ by a single amino acid substitution in each of the hemagglutinin-neuraminidase (HN) and membrane (M) proteins, while their fusion (F) proteins are identical (T. Shioda, S. Wakao, S. Suzu, and H. Sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.9.3661-3668.1989
更新日期:1989-09-01 00:00:00
abstract::In vitro transcription by vesicular stomatitis virus nucleocapsids is inhibited by enzymatic dephosphorylation of the NS protein. We provide evidence that specific, partial dephosphorylation of NS molecules is the only detectable change in nucleocapsids treated with bacterial alkaline phosphatase under conditions that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.1.104-112.1982
更新日期:1982-07-01 00:00:00
abstract::We have presented a new approach to studying bacteriophage T4 head maturation. Using a modified M-band technique, we have shown that progeny deoxyribonucleic acid (DNA) was synthesized on the host cell membrane throughout infection. This DNA was released from the membrane later in infection as the result of formation ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.11.3.359-367.1973
更新日期:1973-03-01 00:00:00
abstract:UNLABELLED:Bean pod mottle virus (BPMV) is a bipartite, positive-sense (+) RNA plant virus in the Secoviridae family. Its RNA1 encodes proteins required for genome replication, whereas RNA2 primarily encodes proteins needed for virion assembly and cell-to-cell movement. However, the function of a 58-kDa protein (P58) e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03301-13
更新日期:2014-03-01 00:00:00
abstract::The existence of novel endogenous retrovirus elements in the chicken genome, designated EAV-HP, with close sequence identity to the env gene of avian leukosis virus (ALV) subgroup J has been reported (L. M. Smith, A. A. Toye, K. Howes, N. Bumstead, L. N. Payne, and K. Venugopal, J. Gen. Virol. 80:261-268, 1999). To re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.3.1296-1306.2000
更新日期:2000-02-01 00:00:00
abstract::Replication by many positive-strand RNA viruses includes genomic RNA amplification and subgenomic mRNA (sgRNA) transcription. For brome mosaic virus (BMV), both processes occur in virus-induced, membrane-associated compartments, require BMV replication factors 1a and 2a, and use negative-strand RNA3 as a template for ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.3.1438-1451.2005
更新日期:2005-02-01 00:00:00
abstract::The techniques of deoxyribonucleic acid-ribonucleic acid (DNA-RNA) hybridization and immunological precipitation were used to compare the synthesis of adenovirus-specific macromolecules in African green monkey kidney (AGMK) cells infected with adenovirus, an abortive infection, and coinfected with both adenovirus and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.6.1109-1117.1972
更新日期:1972-12-01 00:00:00
abstract::The pandemic threat posed by emerging zoonotic influenza A viruses necessitates development of antiviral agents effective against various antigenic subtypes. Human monoclonal antibody (hMAb) targeting the hemagglutinin (HA) stalk offers a promising approach to control influenza virus infections. Here, we investigated ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01284-16
更新日期:2016-11-14 00:00:00
abstract:UNLABELLED:The RNA genome of respiratory syncytial virus (RSV) is constitutively encapsidated by the viral nucleoprotein N, thus forming a helical nucleocapsid. Polymerization of N along the genomic and antigenomic RNAs is concomitant to replication and requires the preservation of an unassembled monomeric nucleoprotei...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03666-14
更新日期:2015-04-01 00:00:00
abstract::Long-term ovine fibroblast cultures contain replicative-competent lentiviruses in a latent form. This in vitro phenomenon, never described previously for lentiviruses, was clearly demonstrated by activating the expression of latent viruses with various inducing cell treatments, some of which were efficient in inducing...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.2.680-682.1984
更新日期:1984-11-01 00:00:00
abstract::The DA strain of Theiler's murine encephalomyelitis virus persists in the white matter of the spinal cords of susceptible mice. Previous results showed that the difference in susceptibility to viral persistence between the susceptible SJL/J strain and the resistant B10.S strain was due to multiple non-H-2 loci. The re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.11.5807-5812.2002
更新日期:2002-06-01 00:00:00
abstract::The E3 complex transcription unit of adenovirus encodes four major mRNAs (a, c, f, and h) and two minor (d and e) mRNAs with overlapping exons, alternative splice sites, and two polyadenylation sites, termed E3A (upstream) and E3B (downstream). mRNAs a and d use the E3A polyadenylation site, and mRNAs c, e, f, and h u...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.10.5914-5923.1992
更新日期:1992-10-01 00:00:00
abstract::There is an incomplete understanding of the differences between neonatal immune responses that contribute to the increased susceptibility of neonates to some viral infections. We tested the hypothesis that neonates are more susceptible than adults to mouse adenovirus type 1 (MAV-1) respiratory infection and are impair...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06967-11
更新日期:2012-04-01 00:00:00
abstract::Epitope-specific CD8+ T lymphocytes may play an important role in controlling human immunodeficiency virus (HIV)/simian immunodeficiency virus replication. Unfortunately, standard cellular assays do not measure the antiviral efficacy (the ability to suppress virus replication) of CD8+ T lymphocytes. Certain epitope-sp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.23.14986-14991.2005
更新日期:2005-12-01 00:00:00
abstract::An mRNA-ribonucleoprotein particle (mRNP) was found in vesicular stomatitis virus (VSV)-infected Chinese hamster ovary cells. The particle was present 3 and 4.5 h after infection but was barely discernible at 2 h. The mRNP (buoyant density, 1.56 g/cm3), which cosedimented with viral nucleocapsid in a sucrose density g...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.3.932-938.1982
更新日期:1982-12-01 00:00:00
abstract::Evidence is presented by use of radiolabeling and pancreatic and T1 ribonuclease digestion that some of the ribonucleic acid specified by herpes simplex virus contains polyadenylic acid sequences. The polyadenylic sequences are not transcribed from viral DNA. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.4.875-879.1972
更新日期:1972-10-01 00:00:00
abstract::Monoclonal antibodies (MAbs) were raised against human immunodeficiency virus type 1 gp120. One MAb, P4/D10, was found to mediate highly efficient antibody-dependent cellular cytotoxicity and virus neutralization. The reactivity was located to a major neutralizing region (amino acids 304 to 323) on gp120. Five other M...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.2.936-940.1990
更新日期:1990-02-01 00:00:00
abstract::The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5. One way in which gPr80 helps MLVs to escape host innate im...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01530-17
更新日期:2018-02-26 00:00:00
abstract::We investigated the protective role of antibodies in vaginal secretions of mice that were immune to vaginal challenge with herpes simplex virus type 2 (HSV-2). Unfractionated vaginal immunoglobulins from immune and nonimmune mice and affinity-purified immunoglobulin G (IgG) and secretory IgA (S-IgA) from immune secret...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8109-8115.1997
更新日期:1997-11-01 00:00:00
abstract::The Kresse strain of porcine parvovirus (PPV) was cloned into pUC19, and independent infectious clones were sequenced. The PPV Kresse and NADL-2 strains, which have different pathogenicities, shared an identical genomic organization and a high degree of sequence identity. Partial genomes (1.5 or 1.6 kb) of 15 field is...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.4.2508-2515.1996
更新日期:1996-04-01 00:00:00
abstract::Envelope glycoprotein E(rns) of classical swine fever virus (CSFV) has been shown to contain RNase activity and is involved in virus infection. Two short regions of amino acids in the sequence of E(rns) are responsible for RNase activity. In both regions, histidine residues appear to be essential for catalysis. They w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.151-157.1998
更新日期:1998-01-01 00:00:00
abstract::In wild aquatic birds and poultry around the world, influenza A viruses carrying 15 antigenic subtypes of hemagglutinin (HA) and 9 antigenic subtypes of neuraminidase (NA) have been described. Here we describe a previously unidentified antigenic subtype of HA (H16), detected in viruses circulating in black-headed gull...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.5.2814-2822.2005
更新日期:2005-03-01 00:00:00
abstract::NF-κB-interacting long noncoding RNA (NKILA) was recently identified as a negative regulator of NF-κB signaling and plays an important role in the development of various cancers. It is well known that NF-κB-mediated activation of human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR)-driven gene expres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01057-20
更新日期:2020-08-17 00:00:00
abstract:UNLABELLED:In vitro, infection of polarized human intestinal epithelial cells by coxsackievirus B3 (CVB3) depends on virus interaction with decay-accelerating factor (DAF), a receptor expressed on the apical cell surface. Although mice are highly susceptible to CVB3 infection when virus is delivered by intraperitoneal ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03468-14
更新日期:2015-04-01 00:00:00
abstract::The mucosal adjuvant effect of synthetic double-stranded RNA polyriboinosinic polyribocytidylic acid [poly(I:C)] against influenza virus was examined under intranasal coadministration with inactivated hemagglutinin (HA) vaccine in BALB/c mice and was shown to have a protective effect against both nasal-restricted infe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.5.2910-2919.2005
更新日期:2005-03-01 00:00:00
abstract::A porcine rotavirus (prv), variant 4F, isolated in tissue culture from the feces of a Chinese pig with diarrhea, was found to have become highly pathogenic when passaged in gnotobiotic piglets (J. C. Bridger, B. Burke, G. M. Beards, and U. Desselberger, J. Gen. Virol. 73:3011-3015, 1992). Comparison with the closely r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.853-857.1997
更新日期:1997-01-01 00:00:00
abstract::Five minority populations of aberrant, varicella-zoster virus (VZV)-derived genomes were identified among the encapsidated DNAs obtained from the nuclear and cytoplasmic fractions of an in vitro infection initiated with a lyophilized sample of the BIKEN VZV vaccine (strain Oka). These were (i) VZV genomes, present wit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.1.92-101.1985
更新日期:1985-10-01 00:00:00
abstract::Simian immunodeficiency virus (SIV) and human immunodeficiency virus (HIV) infections lead to rapid depletion of CD4(+) T cells from gut-associated lymphoid tissue (GALT). Although the administration of antiretroviral therapy (ART) has been shown to increase CD4(+) T-cell levels in the peripheral blood in both SIV and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.5.2709-2719.2005
更新日期:2005-03-01 00:00:00
abstract::Mature nonstructural protein-15 (nsp15) from the severe acute respiratory syndrome coronavirus (SARS-CoV) contains a novel uridylate-specific Mn2+-dependent endoribonuclease (NendoU). Structure studies of the full-length form of the obligate hexameric enzyme from two CoVs, SARS-CoV and murine hepatitis virus, and its ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02817-06
更新日期:2007-06-01 00:00:00