Abstract:
:T7+ phage are unable to plate on a strain of Shigella sonnei D2 371-48. Spontaneous phage mutants arise (ss--mutants) that are able to plate on this strain of Shigella. We have shown by complementation studies and genetic crosses that the ss--mutation maps in gene 10, the structural gene for the major protein of the capsid. This finding implies that the gene 10 protein may interact with a host protein during phage development and that the abortive infection of T7 observed in S. sonnei D2 371-48 with T7+ phage may be a defect in head morphogenesis. Our studies also reveal that various T7 strains commonly contain deletions in nonessential regions. T7 ss--mutants selected after growth of T7+ on Shigella D2 371-48 often acquire a deletion in the 0.7 gene that is not necessary for the ss--phenotype. Finally, we have found a new nonessential region of the T7 chromosome that is located between 33 and 35.5% of the T7 genome length.
journal_name
J Viroljournal_title
Journal of virologyauthors
Vetter D,Roberts L,Jackowski JB,Sadowski PDdoi
10.1128/JVI.22.3.694-701.1977subject
Has Abstractpub_date
1977-06-01 00:00:00pages
694-701issue
3eissn
0022-538Xissn
1098-5514journal_volume
22pub_type
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journal_title:Journal of virology
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