Abstract：
:Mesenchymal stem cells (MSCs) represent a population of cells which have the ability to regulate reactivity of T and B lymphocytes by multiple mechanisms. The immunoregulatory activities of MSCs are strictly influenced by the cytokine environment. Here we show that two functionally distinct cytokines, interleukin-4 (IL-4) and interferon-γ (IFN-γ), significantly potentiate the ability of MSCs to inhibit IL-10 production by activated regulatory B cells (Bregs). However, MSCs in the presence of IL-4 or IFN-γ inhibit the IL-10 production by different mechanisms. Preincubation of MSCs with IFN-γ led to the suppression, but pretreatment with IL-4 of neither MSCs nor B cells resulted in the suppression of IL-10 production. The search for candidate regulatory molecules expressed in cytokine-treated MSCs revealed different patterns of the gene expression. Pretreatment of MSCs with IFN-γ, but not with IL-4, induced expression of indoleamine-2,3-dioxygenase, cyclooxygenase-2 and programmed cell death-ligand 1. To identify the molecule(s) responsible for the suppression of IL-10 production, we used specific inhibitors of the putative regulatory molecules. We found that indomethacine, an inhibitor of cyclooxygenase-2 (Cox-2) activity, completely abrogated the inhibition of IL-10 production in cultures containing MSCs and IFN-γ, but had no effect on the suppression in cell cultures containing MSCs and IL-4. The results show that MSCs can inhibit the response of B cells to one stimulus by different mechanisms in dependence on the cytokine environment and thus support the idea of the complexity of immunoregulatory action of MSCs.
journal_name
Stem Cell Rev Repjournal_title
Stem cell reviews and reportsauthors
Holan V,Hermankova B,Bohacova P,Kossl J,Chudickova M,Hajkova M,Krulova M,Zajicova A,Javorkova Edoi
10.1007/s12015-016-9688-ysubject
Has Abstractpub_date
2016-12-01 00:00:00pages
654-663issue
6eissn
2629-3269issn
2629-3277pii
10.1007/s12015-016-9688-yjournal_volume
12pub_type
杂志文章abstract：:Schwann cells (SC), the glial cells of peripheral nerves, are involved in many diseases including Charcot Marie Tooth and neurofibromatosis, and play a pivotal role in peripheral nerve regeneration. Although it is possible to obtain human SC from nerve biopsies, they are difficult to maintain and expand in culture. He...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
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abstract：:The challenges in limbal stem cell biology largely remain in the process of identification, isolation and expansion of these adult corneal epithelial stem cells of the eye. Due to the absence of specific limbal stem cell markers, identification and isolation of putative limbal stem cells is a complicated task. The sid...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-013-9481-0
更新日期：2014-04-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-010-9160-3
更新日期：2010-09-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
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更新日期：2009-03-01 00:00:00
abstract：:Since its early days, stem cell research, particularly human embryonic stem cell research, has been the focus of intense social debate, and the question of the moral status of the embryo has been a central issue in the controversy. Despite this friction, and while it has yet to obtain widespread success in clinical ap...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
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journal_title：Stem cell reviews and reports
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
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journal_title：Stem cell reviews and reports
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更新日期：2011-03-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
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更新日期：2017-06-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章
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更新日期：2014-10-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-019-09904-4
更新日期：2019-10-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
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更新日期：2015-04-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
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更新日期：2013-06-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
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更新日期：2020-11-17 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
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更新日期：2015-02-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
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journal_title：Stem cell reviews and reports
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pub_type： 杂志文章
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journal_title：Stem cell reviews and reports
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journal_title：Stem cell reviews and reports
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journal_title：Stem cell reviews and reports
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更新日期：2013-02-01 00:00:00
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journal_title：Stem cell reviews and reports
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-019-09936-w
更新日期：2020-04-01 00:00:00
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journal_title：Stem cell reviews and reports
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journal_title：Stem cell reviews and reports
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章
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更新日期：2017-10-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章
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更新日期：2016-10-01 00:00:00
