Abstract：
:Few surface markers are available to monitor lineage differentiation during chondrogenesis. Recently, delta-like1/fetal antigen1 (dlk1/FA1), a transmembrane protein of the Notch/Delta/Serrata family, was shown to be essential for inducing early chondrogenesis. Thus, we investigated the possible use of dlk1/FA1 as a novel surface marker for chondroprogenitor cells during hESC differentiation. We found that, Dlk1/FA1 is expressed specifically in cells undergoing transition from proliferating to prehypertrophic chondrocytes during endochondral ossification of the mouse limb. In hESC cells, dlk1/FA1 was not expressed by undifferentiated hESC, but expressed during in vitro embryoid bodies (hEBs) formation upon down-regulation of undifferentiated markers e.g. Oct 3/4. Similarly, dlk1/FA1 was expressed in chondrocytic cells during in vivo teratoma formation. Interestingly, treatment of hEBs with Activin B, a member of TGF-ss family, markedly increased Dlk1 expression in association with up-regulation of the mesoderm-specific markers (e.g. FOXF1, KDR and VE-cadherin) and SOX9. dlk1/FA1(+) cells isolated by fluorescence activated cell sorting (FACS) were capable of differentiating into chondrocytic cells when cultured as micromass pellets in a xeno-free system containing TGFbeta1. In conclusion, we identified dlk1/FA1 as a novel marker of chondroprogenitor cells that undergo embryonic lineage progression from proliferation to the prehypertrophic stage. Tracking dlk1/FA1 expression as a mesoderm/chondroprogenitor surface marker provides a novel strategy for designing clinically relevant protocols to direct the differentiation of hESC into chondrocytes.
journal_name
Stem Cell Rev Repjournal_title
Stem cell reviews and reportsauthors
Harkness L,Taipaleenmaki H,Mahmood A,Frandsen U,Saamanen AM,Kassem M,Abdallah BMdoi
10.1007/s12015-009-9099-4subject
Has Abstractpub_date
2009-12-01 00:00:00pages
353-68issue
4eissn
2629-3269issn
2629-3277journal_volume
5pub_type
杂志文章abstract：:Retinal degenerative diseases that target photoreceptors or the adjacent retinal pigment epithelium (RPE) affect millions of people worldwide. Retinal degeneration (RD) is found in many different forms of retinal diseases including retinitis pigmentosa (RP), age-related macular degeneration (AMD), diabetic retinopathy...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-010-9192-8
更新日期：2011-06-01 00:00:00
abstract：:Please note the following errors in the original version. ...
journal_title：Stem cell reviews and reports
pub_type： 已发布勘误
doi：10.1007/s12015-018-9849-2
更新日期：2019-02-01 00:00:00
abstract：:Human embryonic stem cells (hESCs) can be propagated indefinitely in vitro in an undifferentiated pluripotent state, can differentiate into derivatives of all three germ layers and are of considerable interest for applications in regenerative medicine. Clinical application of hESCs, however, requires reliable protocol...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-011-9230-1
更新日期：2011-09-01 00:00:00
abstract：:Mesenchymal stem cells (MSCs) represent a population of cells which have the ability to regulate reactivity of T and B lymphocytes by multiple mechanisms. The immunoregulatory activities of MSCs are strictly influenced by the cytokine environment. Here we show that two functionally distinct cytokines, interleukin-4 (I...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-016-9688-y
更新日期：2016-12-01 00:00:00
abstract：:The propensity of induced pluripotent stem (iPS) cells to differentiate into specific lineages may be influenced by a number of factors, including the selection of the somatic cell type used for reprogramming. Herein we report the generation of new iPS cells, which we derived from human articular chondrocytes and from...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-014-9538-8
更新日期：2014-12-01 00:00:00
abstract：:The discovery of induced pluripotent stem (iPS) cells in 2006 marked a major breakthrough in regenerative medicine, enabling reversal of terminally differentiated somatic cells into pluripotent stem cells. The embryonic stem (ES) cells-like pluripotency and unlimited self-renewal capability of iPS cells have granted t...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-020-09956-x
更新日期：2020-04-01 00:00:00
abstract：:Endothelial progenitors are a population of cells with the inherent capacity to differentiate into mature endothelial cells and proangiogenic paracrine action. These characteristics have led to extensive studies being performed and tested in the treatment of tissue ischemia. The natural course of diabetes mellitus (DM...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-018-9863-4
更新日期：2019-04-01 00:00:00
abstract：:Motor neuron loss or degeneration is the typical characteristic of amyotrophic lateral sclerosis (ALS), which often leads to weakness, paralysis, or even death. The underlying mechanisms of motor neuron degeneration and ALS progression remain elusive, and there is no effective treatment for ALS. The advances of stem c...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-019-09946-8
更新日期：2020-06-01 00:00:00
abstract：:The molecular profile of human mesenchymal stem cells (MSCs) have emerged as a key factor in defining their identity. Nevertheless, the effect of fetal bovine serum (FBS) batches or origin on MSC molecular signature has been neglected. In this frame, chemical fingerprint of FBS batches from unrelated countries showed ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-018-9822-0
更新日期：2018-12-01 00:00:00
abstract：:Xenografting of normal and transformed mammalian tissues and cells to chick embryos has been performed for almost 100 years. Embryonic stem cells, derived more than 25 years ago from murine, and more than 10 years ago from human blastocysts, have transformed many fields of biological research. There is a growing body ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-010-9161-2
更新日期：2010-09-01 00:00:00
abstract：:Multipotent mesenchymal stem/stromal cells (MSCs) have regenerative and immunomodulatory properties to restore and repair injured tissues, making them attractive candidates for cell-based therapies. Experimental and clinical evidence has demonstrated the effectiveness of MSC transplantation in managing diabetes mellit...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-018-9869-y
更新日期：2019-04-01 00:00:00
abstract：:Angiotensin-converting enzyme 2 (ACE2) plays an important role as a member of the renin-angiotensin-aldosterone system (RAAS) in regulating the conversion of angiotensin II (Ang II) into angiotensin (1-7) (Ang [1-7]). But at the same time, while expressed on the surface of human cells, ACE2 is the entry receptor for S...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-020-10010-z
更新日期：2020-07-20 00:00:00
abstract：:Traumatic injuries are a leading cause of death and disability in both military and civilian populations. Given the complexity and diversity of traumatic injuries, novel and individualized treatment strategies are required to optimize outcomes. Cellular therapies have potential benefit for the treatment of acute or ch...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-020-10086-7
更新日期：2021-01-08 00:00:00
abstract：:Chemotherapy constitutes one of the key treatment modalities for solid and hematological malignancies. Albeit being an effective treatment, chemotherapy application is often limited by its damage to healthy tissues, and curative treatment options for chemotherapy-related side effects are largely missing. As mesenchyma...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-019-09886-3
更新日期：2019-06-01 00:00:00
abstract：:Stem cells in the endometrium and myometrium possess an immense regenerative potential which is necessary to maintain the menstrual cycle and support pregnancy. These cells, as well as bone marrow stem cells, have also been implicated in the development of common benign gynecological disorders including leiomyomas, en...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-020-10075-w
更新日期：2020-11-05 00:00:00
abstract：:Osteoarthritis (OA) remains incurable in humans or horses and mesenchymal stromal/stem cells (MSCs) represent an attractive solution for producing a neocartilage substitute. However, the best MSC source still needs to be identified. This study compared the chondrogenic potential of equine MSCs derived from bone marrow...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-019-09914-2
更新日期：2020-02-01 00:00:00
abstract：:The ROCK inhibitor Y-27632 inhibits apoptosis and increases proliferation of frozen-thawed cells. We examined the role of Y-27632 on human umbilical cord Wharton's jelly stem cells (hWJSCs) for (1) thaw-survival (2) proliferation and (3) preservation of stemness and differentiation potential after cryopreservation. hW...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-010-9184-8
更新日期：2010-12-01 00:00:00
abstract：:Here we present the principles and steps of a protocol that we have recently developed for the differentiation of hES/iPS cells into the authentic human striatal projection medium spiny neurons (MSNs) that die in Huntington's Disease (HD). Authenticity is judged by the convergence of multiple features within individua...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-013-9441-8
更新日期：2013-08-01 00:00:00
abstract：:Zinc-finger nucleases (ZFNs) are sequence-specific genome engineering tools with great potential for the development of gene therapies. The achievement of permanent cures through gene therapy requires targeting of stem cells but the effects and/or side effects of ZFN treatment on adult stem cell potency are largely un...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-011-9313-z
更新日期：2012-06-01 00:00:00
abstract：:Metastasis is the worst prognosis predictor in the clinical course of cancer development. Features of metastatic cancer cells include migratory ability, low degree of differentiation, self-renewal and proliferation potentials, as well as resistance to therapies. Metastatic cells do not present all of the necessary cha...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-019-09936-w
更新日期：2020-04-01 00:00:00
abstract：:During the process of development, neural crest cells migrate out from their niche between the newly formed ectoderm and the neural tube. Thereafter, they give rise not only to ectodermal cell types, but also to mesodermal cell types. Cell types with neural crest ancestry consequently comprise a number of specialized ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-011-9340-9
更新日期：2012-09-01 00:00:00
abstract：:Neural progenitor cells (NPCs) are multipotent cells that have the potential to produce neurons and glial cells in the neural system. NPCs undergo identity maintenance or differentiation regulated by different kinds of transcription factors. Here we present evidence that ETV5, which is an ETS transcription factor, pro...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-019-09904-4
更新日期：2019-10-01 00:00:00
abstract：INTRODUCTION:Whether transplanted cardiac stem cells (CSCs) and mesenchymal stem cells (MSCs) improved ventricular fibrillation threshold (VFT) similarly is still unclear. We sought to compare the effects of the CSC and MSC transplantation on the electrophysiological characteristics and VFT in rats with myocardial infa...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-012-9367-6
更新日期：2013-06-01 00:00:00
abstract：:The combination of cell therapy with growth factors could be a useful approach to treat progressive muscular dystrophies. Here, we demonstrate, for the first time, that IGF-1 considerably enhances the myogenesis of human umbilical cord (UC) mesenchymal stromal cells (MSCs) in vitro and that IGF-1 enhances interaction ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-012-9380-9
更新日期：2013-02-01 00:00:00
abstract：:Epicardial resident stem cells are known to differentiate into cardiomyocytes during cardiac development, amongst other cell types. Whether epicardium-derived progenitor cells (EPDCs) retain this plasticity in the adult heart has been the topic of heated scientific debate. Priming with thymosin beta 4, a peptide which...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-012-9378-3
更新日期：2013-06-01 00:00:00
abstract：:Recent advances on human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have brought us closer to the realization of their clinical potential. Nonetheless, tissue engineering and regenerative medicine applications will require the generation of ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-014-9544-x
更新日期：2015-02-01 00:00:00
abstract：:Schwann cells (SC), the glial cells of peripheral nerves, are involved in many diseases including Charcot Marie Tooth and neurofibromatosis, and play a pivotal role in peripheral nerve regeneration. Although it is possible to obtain human SC from nerve biopsies, they are difficult to maintain and expand in culture. He...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-010-9198-2
更新日期：2011-06-01 00:00:00
abstract：:The challenges in limbal stem cell biology largely remain in the process of identification, isolation and expansion of these adult corneal epithelial stem cells of the eye. Due to the absence of specific limbal stem cell markers, identification and isolation of putative limbal stem cells is a complicated task. The sid...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-013-9481-0
更新日期：2014-04-01 00:00:00
abstract：BACKGROUND:Bone marrow-derived mononuclear cells (BM-MNC) consist of a heterogeneous mix of mesenchymal stem cells (MSC), hematopoietic progenitor cells (HPC), endothelial progenitor cells (EPC), monocytes, lymphocytes and pluripotent stem cells. Whereas the importance of MSC and EPC has been well documented in bone he...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-019-09943-x
更新日期：2020-02-01 00:00:00
abstract：:Although bone marrow-derived mononuclear cells (BMNC) have been extensively used in cell therapy for cardiac diseases, little mechanistic information is available to support reports of their efficacy. To address this shortcoming, we compared structural and functional recovery and associated global gene expression prof...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-011-9282-2
更新日期：2012-03-01 00:00:00
