Abstract:
:Human embryonic stem cells (hESCs) can be propagated indefinitely in vitro in an undifferentiated pluripotent state, can differentiate into derivatives of all three germ layers and are of considerable interest for applications in regenerative medicine. Clinical application of hESCs, however, requires reliable protocols for cryopreservation. Current protocols for cryopreservation of hESCs suffer from low recovery rates of hESCs and loss of pluripotency after thawing. We therefore studied the effects of cryopreservation on the viability, proliferation potential, and the pluripotency status of hESCs by combining cellular readouts and transcriptomics. We identified biological processes and pathways affected by cryopreservation in order to understand the limited survival rate of hESCs by comparing transcriptomes of hESCs at different time points after thawing with cells that did not undergo cryopreservation. While the transcriptomes of cells post thawing were very similar to those of control non-frozen hESCs for the early time points, we observed increased expression of genes involved in apoptosis, embryonic morphogenesis, ossification, tissue morphogenesis, regeneration, vasculature development and cell death at later time points. Our data suggest that inhibition of anoikis apoptosis and the stress-induced differentiation pathways are promising targets for improving the survival rate and maintaining pluripotency of hESCs after cryopreservation.
journal_name
Stem Cell Rev Repjournal_title
Stem cell reviews and reportsauthors
Wagh V,Meganathan K,Jagtap S,Gaspar JA,Winkler J,Spitkovsky D,Hescheler J,Sachinidis Adoi
10.1007/s12015-011-9230-1subject
Has Abstractpub_date
2011-09-01 00:00:00pages
506-17issue
3eissn
2629-3269issn
2629-3277journal_volume
7pub_type
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journal_title:Stem cell reviews and reports
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pub_type: 杂志文章,评审
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journal_title:Stem cell reviews and reports
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journal_title:Stem cell reviews and reports
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更新日期:2017-10-01 00:00:00
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-017-9751-3
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-016-9669-1
更新日期:2016-10-01 00:00:00
abstract::The propensity of induced pluripotent stem (iPS) cells to differentiate into specific lineages may be influenced by a number of factors, including the selection of the somatic cell type used for reprogramming. Herein we report the generation of new iPS cells, which we derived from human articular chondrocytes and from...
journal_title:Stem cell reviews and reports
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
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更新日期:2018-04-01 00:00:00
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-017-9766-9
更新日期:2017-12-01 00:00:00
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-015-9609-5
更新日期:2015-12-01 00:00:00
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-016-9688-y
更新日期:2016-12-01 00:00:00
abstract::Multipotent mesenchymal stem/stromal cells (MSCs) have regenerative and immunomodulatory properties to restore and repair injured tissues, making them attractive candidates for cell-based therapies. Experimental and clinical evidence has demonstrated the effectiveness of MSC transplantation in managing diabetes mellit...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-018-9869-y
更新日期:2019-04-01 00:00:00
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-019-09914-2
更新日期:2020-02-01 00:00:00
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更新日期:2010-09-01 00:00:00
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-014-9564-6
更新日期:2015-08-01 00:00:00
abstract::The combination of cell therapy with growth factors could be a useful approach to treat progressive muscular dystrophies. Here, we demonstrate, for the first time, that IGF-1 considerably enhances the myogenesis of human umbilical cord (UC) mesenchymal stromal cells (MSCs) in vitro and that IGF-1 enhances interaction ...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-012-9380-9
更新日期:2013-02-01 00:00:00
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-011-9256-4
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-014-9531-2
更新日期:2014-10-01 00:00:00
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-014-9554-8
更新日期:2015-02-01 00:00:00
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-017-9798-1
更新日期:2018-04-01 00:00:00
abstract:BACKGROUND:Mesenchymal stem cells derived from the chorionic villi of human term placenta (pMSCs) have drawn considerable interest because of their multipotent differentiation potential and their immunomodulatory capacity. These properties are the foundation for their clinical application in the fields of stem cell tra...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-014-9562-8
更新日期:2015-06-01 00:00:00
abstract::The COVID-19 pandemic has profoundly influenced public health and contributed to global economic divergences of unprecedented dimensions. Due to the high prevalence and mortality rates, it is then expected that the consequence and public health challenges will last for long periods. The rapid global spread of COVID-19...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-020-10108-4
更新日期:2021-01-05 00:00:00
abstract::Factors responsible for the rapid proliferative properties of embryonic stem (ES) cells are largely unknown. MicroRNA-221/222 (miR-221/222) regulate proliferation in many somatic cells, however, their roles in proliferation of ES cells are unclear. In this study, E14 mouse ES cells proliferation was determined by tota...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-014-9543-y
更新日期:2015-02-01 00:00:00
abstract::The interest in the use of stem cells as a source for therapy has increased dramatically over the last decades. Different stem cell types have been tested in both in vitro and in vivo models, because of their properties such as differentiation potential, trophic effects and immune modulatory properties. To further opt...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-014-9575-3
更新日期:2015-04-01 00:00:00
abstract::Reliable and efficient DNA and RNA transfection methods are required when studying the role of individual genes in mouse pluripotent stem cells. However, these cells usually grow in tight clusters and are therefore more difficult to transfect than many other cell lines. We have found that transfection is especially ch...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-016-9673-5
更新日期:2016-10-01 00:00:00
abstract::Hematopoietic stem cells (HSCs) from umbilical cord blood have been successfully used to treat blood disorders but one major hurdle is the relatively low cell dose available. Double cord blood unit transplantation results in elevated engraftment failure because one unit predominates over the other. Various approaches ...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-013-9426-7
更新日期:2013-04-01 00:00:00
abstract::Converting adult cells from one cell type to another is a particularly interesting idea for regenerative medicine. Terminally differentiated cells can be induced to de-differentiate in vitro to become multipotent progenitors. In mammals these changes do not occur naturally, however exposing differentiated adult cells ...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-014-9521-4
更新日期:2014-10-01 00:00:00