Abstract:
:Idiopathic pulmonary fibrosis (IPF) is a devastating disease characterized by obliteration of alveolar architecture, resulting in declining lung function and ultimately death. Pathogenic mechanisms remain unclear but involve a concomitant accumulation of scar tissue together with myofibroblasts activation. Microparticles (MPs) have been investigated in several human lung diseases as possible pathogenic elements, prognosis markers and therapeutic targets. We postulated that levels and cellular origins of circulating MPs might serve as biomarkers in IPF patients and/or as active players of fibrogenesis. Flow cytometry analysis showed a higher level of Annexin-V positive endothelial and platelet MPs in 41 IPF patients compared to 22 healthy volunteers. Moreover, in IPF patients with a low diffusing capacity of the lung for carbon monoxide (DLCO<40%), endothelial MPs (EMPs) were found significantly higher compared to those with DLCO>40% (p = 0.02). We then used EMPs isolated from endothelial progenitor cells (ECFCs) extracted from IPF patients or controls to modulate normal human lung fibroblast (NHLF) properties. We showed that EMPs did not modify proliferation, collagen deposition and myofibroblast transdifferentiation. However, EMPs from IPF patients stimulated migration capacity of NHLF. We hypothesized that this effect could result from EMPs fibrinolytic properties and found indeed higher plasminogen activation potential in total circulating MPs and ECFCs derived MPs issued from IPF patients compared to those isolated from healthy controls MPs. Our study showed that IPF is associated with an increased level of EMPs in the most severe patients, highlighting an active process of endothelial activation in the latter. Endothelial microparticles might contribute to the lung fibroblast invasion mediated, at least in part, by a fibrinolytic activity.
journal_name
Stem Cell Rev Repjournal_title
Stem cell reviews and reportsauthors
Bacha NC,Blandinieres A,Rossi E,Gendron N,Nevo N,Lecourt S,Guerin CL,Renard JM,Gaussem P,Angles-Cano E,Boulanger CM,Israel-Biet D,Smadja DMdoi
10.1007/s12015-017-9778-5subject
Has Abstractpub_date
2018-04-01 00:00:00pages
223-235issue
2eissn
2629-3269issn
2629-3277pii
10.1007/s12015-017-9778-5journal_volume
14pub_type
杂志文章abstract::Mesenchymal stem cells (MSC) have been extensively studied over the past years for the treatment of different diseases. Most of the ongoing clinical trials currently involve the use of MSC derived from adult tissues. This source may have some limitations, particularly with therapies that may require extensive and repe...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-016-9695-z
更新日期:2017-02-01 00:00:00
abstract::The vertebrate early stage embryo is consisting of the three primary germ layers ectoderm, mesoderm and endoderm, from which all organ tissues are developed. During early embryonic development, mesodermal cells become sequentially determined to more precisely defined cell types including muscle, heart, vasculature, bl...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-011-9263-5
更新日期:2012-03-01 00:00:00
abstract::Mesenchymal stromal cells (MSCs) constitute a heterogeneous population of stromal cells with immunomodulatory and regenerative properties that support their therapeutic use. MSCs isolated from many tissue sources replicate vigorously in vitro and maintain their main biological properties allowing their widespread clin...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-020-10032-7
更新日期:2020-09-07 00:00:00
abstract::The use of mesenchymal stem/stromal cells (MSC) in regenerative medicine often requires MSC to function in environments of high oxidative stress. Human pregnancy is a condition where the mother's tissues, and in particular her circulatory system, are exposed to increased levels of oxidative stress. MSC in the maternal...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-016-9649-5
更新日期:2016-06-01 00:00:00
abstract::The morphology of several tumors mimics developmentally early tissues; tumors often express early developmental markers characteristic for the germ line lineage. Recently, our group identified a population of very small stem cells (SCs) in murine bone marrow (BM) and other adult organs that express several markers cha...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-010-9143-4
更新日期:2010-06-01 00:00:00
abstract::Epicardial resident stem cells are known to differentiate into cardiomyocytes during cardiac development, amongst other cell types. Whether epicardium-derived progenitor cells (EPDCs) retain this plasticity in the adult heart has been the topic of heated scientific debate. Priming with thymosin beta 4, a peptide which...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-012-9378-3
更新日期:2013-06-01 00:00:00
abstract::The discovery of induced pluripotent stem (iPS) cells in 2006 marked a major breakthrough in regenerative medicine, enabling reversal of terminally differentiated somatic cells into pluripotent stem cells. The embryonic stem (ES) cells-like pluripotency and unlimited self-renewal capability of iPS cells have granted t...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-020-09956-x
更新日期:2020-04-01 00:00:00
abstract:BACKGROUND:Mesenchymal stem cells derived from the chorionic villi of human term placenta (pMSCs) have drawn considerable interest because of their multipotent differentiation potential and their immunomodulatory capacity. These properties are the foundation for their clinical application in the fields of stem cell tra...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-014-9562-8
更新日期:2015-06-01 00:00:00
abstract::The absence of effective and satisfactory treatments that contribute to repairing the dopaminergic damage caused by Parkinson's Disease (PD) and the limited recovery capacity of the nervous system are troubling issues and the focus of many research and clinical domains. Recent advances in the treatment of PD through s...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-017-9798-1
更新日期:2018-04-01 00:00:00
abstract::The inward rectifier potassium current (IK1) is generally thought to suppress cardiac automaticity by hyperpolarizing membrane potential (MP). We recently observed that IK1 could promote the spontaneously-firing automaticity induced by upregulation of pacemaker funny current (If) in adult ventricular cardiomyocytes (C...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-017-9745-1
更新日期:2017-10-01 00:00:00
abstract::Traumatic injuries are a leading cause of death and disability in both military and civilian populations. Given the complexity and diversity of traumatic injuries, novel and individualized treatment strategies are required to optimize outcomes. Cellular therapies have potential benefit for the treatment of acute or ch...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-020-10086-7
更新日期:2021-01-08 00:00:00
abstract::Neural progenitor cells (NPCs) are multipotent cells that have the potential to produce neurons and glial cells in the neural system. NPCs undergo identity maintenance or differentiation regulated by different kinds of transcription factors. Here we present evidence that ETV5, which is an ETS transcription factor, pro...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-019-09904-4
更新日期:2019-10-01 00:00:00
abstract::Cell-based therapy is a promising approach for many diseases, including ischemic heart disease. Cardiac mesoangioblasts are committed vessel-associated progenitors that can restore to a significant, although partial, extent, heart structure and function in a murine model of myocardial infarction. Low-intensity pulsed ...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-015-9608-6
更新日期:2015-12-01 00:00:00
abstract::Converting adult cells from one cell type to another is a particularly interesting idea for regenerative medicine. Terminally differentiated cells can be induced to de-differentiate in vitro to become multipotent progenitors. In mammals these changes do not occur naturally, however exposing differentiated adult cells ...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-014-9521-4
更新日期:2014-10-01 00:00:00
abstract::Recent advances on human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have brought us closer to the realization of their clinical potential. Nonetheless, tissue engineering and regenerative medicine applications will require the generation of ...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-014-9544-x
更新日期:2015-02-01 00:00:00
abstract::Oligodendrocyte progenitor cells (OPCs) play a pivotal role in both health and disease within the central nervous system, with oligodendrocytes, arising from resident OPCs, being the main myelinating cell type. Disruption in OPC numbers can lead to various deleterious health defects. Numerous studies have described te...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-017-9742-4
更新日期:2017-08-01 00:00:00
abstract::The combination of cell therapy with growth factors could be a useful approach to treat progressive muscular dystrophies. Here, we demonstrate, for the first time, that IGF-1 considerably enhances the myogenesis of human umbilical cord (UC) mesenchymal stromal cells (MSCs) in vitro and that IGF-1 enhances interaction ...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-012-9380-9
更新日期:2013-02-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) are adult, self-renewable, multipotent cells that can be found in almost all postnatal tissues. Because of their capacity for self-renewal and differentiation into tissues of mesodermal origin and due to their immunomodulatory ability, MSCs are used in many preclinical and clinical studie...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-014-9583-3
更新日期:2015-04-01 00:00:00
abstract::Retinal degenerative diseases that target photoreceptors or the adjacent retinal pigment epithelium (RPE) affect millions of people worldwide. Retinal degeneration (RD) is found in many different forms of retinal diseases including retinitis pigmentosa (RP), age-related macular degeneration (AMD), diabetic retinopathy...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-010-9192-8
更新日期:2011-06-01 00:00:00
abstract:PURPOSE:Evaluation of potential tropic effects of vascular endothelial growth factor (VEGF) on the incorporation and differentiation of bone-marrow-derived stem cells (BMSCs) in a murine model of anterior ischemic optic neuropathy (AION). METHODS:In the first approach, small-sized subset of BMCs were isolated from GFP...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-012-9396-1
更新日期:2012-12-01 00:00:00
abstract::Islet transplantation has been hampered by the shortage of islet donors available for diabetes therapy. However, pluripotent stem cells (PSCs) can be an alternative source of insulin-producing cells (IPCs) because of their capacity for self-renewal and differentiation. We described a method to efficiently differentiat...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-014-9554-8
更新日期:2015-02-01 00:00:00
abstract::We previously showed the emergence of predominantly non-fused murine cells co-expressing cardiac and stromal determinants in co-cultures of murine mesenchymal stromal cells (MSCs) and rat embryonic cardiomyocytes. To determine whether a similar phenotype is detectable in vivo in ischemic myocardium, we infused green f...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-013-9483-y
更新日期:2014-04-01 00:00:00
abstract::Understanding the genetic and molecular mechanisms of ovarian cancer has been the focus of research efforts working toward the greater goal of improving cancer therapy for patients with residual disease after initial treatment with conventional surgery and neoadjuvant chemotherapy. The focus of this review will be cen...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-011-9344-5
更新日期:2012-09-01 00:00:00
abstract::Few surface markers are available to monitor lineage differentiation during chondrogenesis. Recently, delta-like1/fetal antigen1 (dlk1/FA1), a transmembrane protein of the Notch/Delta/Serrata family, was shown to be essential for inducing early chondrogenesis. Thus, we investigated the possible use of dlk1/FA1 as a no...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-009-9099-4
更新日期:2009-12-01 00:00:00
abstract::During the process of development, neural crest cells migrate out from their niche between the newly formed ectoderm and the neural tube. Thereafter, they give rise not only to ectodermal cell types, but also to mesodermal cell types. Cell types with neural crest ancestry consequently comprise a number of specialized ...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-011-9340-9
更新日期:2012-09-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) represent a population of cells which have the ability to regulate reactivity of T and B lymphocytes by multiple mechanisms. The immunoregulatory activities of MSCs are strictly influenced by the cytokine environment. Here we show that two functionally distinct cytokines, interleukin-4 (I...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-016-9688-y
更新日期:2016-12-01 00:00:00
abstract::Although the stem cells are commonly isolated by FACS or MACS, they are very expensive and these is no specific marker for liver stem/progentior cells (LSPCs). This paper applied a convenient and efficient method to enrich LSPCs. The fetal liver cells (FLCs) were firstly enriched by Percoll discontinuous gradient cent...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-010-9119-4
更新日期:2011-03-01 00:00:00
abstract::The challenges in limbal stem cell biology largely remain in the process of identification, isolation and expansion of these adult corneal epithelial stem cells of the eye. Due to the absence of specific limbal stem cell markers, identification and isolation of putative limbal stem cells is a complicated task. The sid...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-013-9481-0
更新日期:2014-04-01 00:00:00
abstract::Osteoarthritis (OA) remains incurable in humans or horses and mesenchymal stromal/stem cells (MSCs) represent an attractive solution for producing a neocartilage substitute. However, the best MSC source still needs to be identified. This study compared the chondrogenic potential of equine MSCs derived from bone marrow...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-019-09914-2
更新日期:2020-02-01 00:00:00
abstract::Here we present the principles and steps of a protocol that we have recently developed for the differentiation of hES/iPS cells into the authentic human striatal projection medium spiny neurons (MSNs) that die in Huntington's Disease (HD). Authenticity is judged by the convergence of multiple features within individua...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-013-9441-8
更新日期:2013-08-01 00:00:00