Abstract：
:Although the stem cells are commonly isolated by FACS or MACS, they are very expensive and these is no specific marker for liver stem/progentior cells (LSPCs). This paper applied a convenient and efficient method to enrich LSPCs. The fetal liver cells (FLCs) were firstly enriched by Percoll discontinuous gradient centrifugation (PDGC) from the rat fetal liver. Then the FLCs in culture were purified to be homogeneous in size by differential trypsinization and differential adherence (DTDA). Flow cytometric analysis revealed more than half of the purified FLCs expressed alternative markers of LSPCs (CD117, c-Met, Sca-1, CD90, CD49f and CD133). In other words, the purified FLCs were heterogeneous. Therefore, they were sequentially layered into six fractions by Percoll continuous gradient centrifugation (PCGC). Both CD133 and CD49f expressed decreasingly from fraction 1 to 6. In fraction 1 and 2, about 85% FLCs expressed CD133, which were revealed to be LSPCs by high expressions of AFP and CK-19, low expressions of G-6-P and ALB. To conclude, the purity of CD133(+) LSPCs enriched by combination of PDGC, DTDA and PCGC is close to that obtained by MACS. This study will greatly contribute to two important biological aspects: liver stem cells isolation and liver cell therapy.
journal_name
Stem Cell Rev Repjournal_title
Stem cell reviews and reportsauthors
Liu WH,Li R,Dou KFdoi
10.1007/s12015-010-9119-4subject
Has Abstractpub_date
2011-03-01 00:00:00pages
94-102issue
1eissn
2629-3269issn
2629-3277journal_volume
7pub_type
杂志文章abstract：:The original version of this article unfortunately contained a mistake. In the author group, the correct family name of Dr. Rebeca is "Blázquez" and the correct family name of Dr. Francisco Miguel is "Sánchez-Margallo." ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,已发布勘误
doi：10.1007/s12015-020-09962-z
更新日期：2020-06-01 00:00:00
abstract：:Few surface markers are available to monitor lineage differentiation during chondrogenesis. Recently, delta-like1/fetal antigen1 (dlk1/FA1), a transmembrane protein of the Notch/Delta/Serrata family, was shown to be essential for inducing early chondrogenesis. Thus, we investigated the possible use of dlk1/FA1 as a no...
journal_title：Stem cell reviews and reports
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
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abstract：:The combination of cell therapy with growth factors could be a useful approach to treat progressive muscular dystrophies. Here, we demonstrate, for the first time, that IGF-1 considerably enhances the myogenesis of human umbilical cord (UC) mesenchymal stromal cells (MSCs) in vitro and that IGF-1 enhances interaction ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-012-9380-9
更新日期：2013-02-01 00:00:00
abstract：:Undifferentiated mouse embryonic stem cells (ESCs) possess low numbers of mitochondrial DNA (mtDNA), which encodes key subunits associated with the generation of ATP through oxidative phosphorylation (OXPHOS). As ESCs differentiate, mtDNA copy number is regulated by the nuclear-encoded mtDNA replication factors, which...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
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更新日期：2017-06-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-016-9693-1
更新日期：2017-02-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
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更新日期：2021-01-08 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-013-9490-z
更新日期：2014-04-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-014-9554-8
更新日期：2015-02-01 00:00:00
abstract：:Please note the following errors in the original version. ...
journal_title：Stem cell reviews and reports
pub_type： 已发布勘误
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更新日期：2019-02-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-013-9426-7
更新日期：2013-04-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-017-9778-5
更新日期：2018-04-01 00:00:00
abstract：:Orthotopic liver transplant (OLT) remains the standard of care for end stage liver disease. To circumvent allo-rejection, OLT subjects receive gluococorticoids (GC). We investigated the effects of GC on endogenous mesenchymal stem (stromal) cells (MSCs) in OLT. This question is relevant because MSCs have regenerative ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
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更新日期：2017-10-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章
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更新日期：2012-06-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-020-09956-x
更新日期：2020-04-01 00:00:00
abstract：:The use of mesenchymal stem/stromal cells (MSC) in regenerative medicine often requires MSC to function in environments of high oxidative stress. Human pregnancy is a condition where the mother's tissues, and in particular her circulatory system, are exposed to increased levels of oxidative stress. MSC in the maternal...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-016-9649-5
更新日期：2016-06-01 00:00:00
abstract：:Xenografting of normal and transformed mammalian tissues and cells to chick embryos has been performed for almost 100 years. Embryonic stem cells, derived more than 25 years ago from murine, and more than 10 years ago from human blastocysts, have transformed many fields of biological research. There is a growing body ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-010-9161-2
更新日期：2010-09-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
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更新日期：2019-04-01 00:00:00
abstract：PURPOSE:Evaluation of potential tropic effects of vascular endothelial growth factor (VEGF) on the incorporation and differentiation of bone-marrow-derived stem cells (BMSCs) in a murine model of anterior ischemic optic neuropathy (AION). METHODS:In the first approach, small-sized subset of BMCs were isolated from GFP...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
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更新日期：2012-12-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
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更新日期：2017-12-01 00:00:00
abstract：:The molecular profile of human mesenchymal stem cells (MSCs) have emerged as a key factor in defining their identity. Nevertheless, the effect of fetal bovine serum (FBS) batches or origin on MSC molecular signature has been neglected. In this frame, chemical fingerprint of FBS batches from unrelated countries showed ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-018-9822-0
更新日期：2018-12-01 00:00:00
abstract：:The morphology of several tumors mimics developmentally early tissues; tumors often express early developmental markers characteristic for the germ line lineage. Recently, our group identified a population of very small stem cells (SCs) in murine bone marrow (BM) and other adult organs that express several markers cha...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-010-9143-4
更新日期：2010-06-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-009-9062-4
更新日期：2009-06-01 00:00:00
abstract：:Osteoarthritis (OA) remains incurable in humans or horses and mesenchymal stromal/stem cells (MSCs) represent an attractive solution for producing a neocartilage substitute. However, the best MSC source still needs to be identified. This study compared the chondrogenic potential of equine MSCs derived from bone marrow...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
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更新日期：2020-02-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-010-9174-x
更新日期：2010-12-01 00:00:00
abstract：:Human embryonic stem cells (hESCs) can be propagated indefinitely in vitro in an undifferentiated pluripotent state, can differentiate into derivatives of all three germ layers and are of considerable interest for applications in regenerative medicine. Clinical application of hESCs, however, requires reliable protocol...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
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更新日期：2011-09-01 00:00:00
abstract：:Angiotensin-converting enzyme 2 (ACE2) plays an important role as a member of the renin-angiotensin-aldosterone system (RAAS) in regulating the conversion of angiotensin II (Ang II) into angiotensin (1-7) (Ang [1-7]). But at the same time, while expressed on the surface of human cells, ACE2 is the entry receptor for S...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-020-10010-z
更新日期：2020-07-20 00:00:00
abstract：:Mesenchymal stem cells (MSCs) are adult, self-renewable, multipotent cells that can be found in almost all postnatal tissues. Because of their capacity for self-renewal and differentiation into tissues of mesodermal origin and due to their immunomodulatory ability, MSCs are used in many preclinical and clinical studie...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
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更新日期：2015-04-01 00:00:00
