Abstract：
:Islet transplantation has been hampered by the shortage of islet donors available for diabetes therapy. However, pluripotent stem cells (PSCs) can be an alternative source of insulin-producing cells (IPCs) because of their capacity for self-renewal and differentiation. We described a method to efficiently differentiate PSCs into IPCs by co-culturing mature islets with directed-differentiated pancreatic endoderm (PE) cells from mouse and human PSCs. PE cells co-cultured with islet cells or islet cell-derived conditioned medium (CM) showed increased expression levels of β-cell markers; significantly higher levels of proinsulin- and Newport Green (NG)-positive cells, which revealed the characteristics of insulin producing cells; and increased insulin secretion upon glucose stimulation. Co-culturing human PE cells with islet cells was also effective to differentiate PE cells into IPCs. Diabetic nude mice transplanted with co-cultured cells exhibited restored euglycemia, human C-peptide release, and improved glucose tolerance. Immunohistochemistry revealed that insulin+/C-peptide + cells existed in the grafted tissues. These results suggest that mature islet cells can increase the differentiation efficiency of PE cells into mature IPCs via paracrine effects.
journal_name
Stem Cell Rev Repjournal_title
Stem cell reviews and reportsauthors
Oh BJ,Oh SH,Choi JM,Jin SM,Shim WY,Lee MS,Lee MK,Kim KW,Kim JHdoi
10.1007/s12015-014-9554-8subject
Has Abstractpub_date
2015-02-01 00:00:00pages
62-74issue
1eissn
2629-3269issn
2629-3277journal_volume
11pub_type
杂志文章abstract：:Mesenchymal stem cells (MSCs) are promising tools for studying the mechanisms of development and for the regeneration of injured tissues. Correct selection of the MSCs source is crucial in order to obtain a more efficient treatment and, in this respect Periosteum-Derived Cells (PDPCs) may represent an interesting alte...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-012-9374-7
更新日期：2012-09-01 00:00:00
abstract：PURPOSE:Evaluation of potential tropic effects of vascular endothelial growth factor (VEGF) on the incorporation and differentiation of bone-marrow-derived stem cells (BMSCs) in a murine model of anterior ischemic optic neuropathy (AION). METHODS:In the first approach, small-sized subset of BMCs were isolated from GFP...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-012-9396-1
更新日期：2012-12-01 00:00:00
abstract：:The stem-cell-based tissue-engineering approaches are widely applied in establishing functional organs and tissues for regenerative medicine. Successful generation of induced pluripotent stem cells (iPS cells) and rapid progress of related technical platform provide great promise in the development of regenerative med...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-014-9531-2
更新日期：2014-10-01 00:00:00
abstract：:Mesenchymal stem cells (MSCs) represent a population of cells which have the ability to regulate reactivity of T and B lymphocytes by multiple mechanisms. The immunoregulatory activities of MSCs are strictly influenced by the cytokine environment. Here we show that two functionally distinct cytokines, interleukin-4 (I...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-016-9688-y
更新日期：2016-12-01 00:00:00
abstract：BACKGROUND:Bone marrow-derived mononuclear cells (BM-MNC) consist of a heterogeneous mix of mesenchymal stem cells (MSC), hematopoietic progenitor cells (HPC), endothelial progenitor cells (EPC), monocytes, lymphocytes and pluripotent stem cells. Whereas the importance of MSC and EPC has been well documented in bone he...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-019-09943-x
更新日期：2020-02-01 00:00:00
abstract：:Orthotopic liver transplant (OLT) remains the standard of care for end stage liver disease. To circumvent allo-rejection, OLT subjects receive gluococorticoids (GC). We investigated the effects of GC on endogenous mesenchymal stem (stromal) cells (MSCs) in OLT. This question is relevant because MSCs have regenerative ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-017-9751-3
更新日期：2017-10-01 00:00:00
abstract：:Various strategies have been published enabling cardiomyocyte differentiation of human induced pluripotent stem (iPS) cells. However the complex nature of signaling pathways involved as well as line-to-line variability compromises the application of a particular protocol to robustly obtain cardiomyocytes from multiple...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-014-9564-6
更新日期：2015-08-01 00:00:00
abstract：:Previous studies demonstrated that induced pluripotent stem (iPS) cells could produce viable mice through tetraploid complementation, which was thought to be the most stringent test for pluripotency. However, these highly pluripotent iPS cells were previously reported to be generated from fibroblasts of embryonic orig...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-010-9160-3
更新日期：2010-09-01 00:00:00
abstract：:Cell-based therapy is a promising approach for many diseases, including ischemic heart disease. Cardiac mesoangioblasts are committed vessel-associated progenitors that can restore to a significant, although partial, extent, heart structure and function in a murine model of myocardial infarction. Low-intensity pulsed ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-015-9608-6
更新日期：2015-12-01 00:00:00
abstract：:Reliable and efficient DNA and RNA transfection methods are required when studying the role of individual genes in mouse pluripotent stem cells. However, these cells usually grow in tight clusters and are therefore more difficult to transfect than many other cell lines. We have found that transfection is especially ch...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-016-9673-5
更新日期：2016-10-01 00:00:00
abstract：:Osteoarthritis (OA) remains incurable in humans or horses and mesenchymal stromal/stem cells (MSCs) represent an attractive solution for producing a neocartilage substitute. However, the best MSC source still needs to be identified. This study compared the chondrogenic potential of equine MSCs derived from bone marrow...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-019-09914-2
更新日期：2020-02-01 00:00:00
abstract：:Endothelial progenitors are a population of cells with the inherent capacity to differentiate into mature endothelial cells and proangiogenic paracrine action. These characteristics have led to extensive studies being performed and tested in the treatment of tissue ischemia. The natural course of diabetes mellitus (DM...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-018-9863-4
更新日期：2019-04-01 00:00:00
abstract：:In this report we review the concept of standardization and propose an exhaustive framework for the proper management of technology on pluripotent stem cells based on studies of global and regional initiatives. We demonstrated detailed observational analysis on global initiatives for the standardization of related tec...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-010-9204-8
更新日期：2011-06-01 00:00:00
abstract：:Zinc-finger nucleases (ZFNs) are sequence-specific genome engineering tools with great potential for the development of gene therapies. The achievement of permanent cures through gene therapy requires targeting of stem cells but the effects and/or side effects of ZFN treatment on adult stem cell potency are largely un...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-011-9313-z
更新日期：2012-06-01 00:00:00
abstract：:The morphology of several tumors mimics developmentally early tissues; tumors often express early developmental markers characteristic for the germ line lineage. Recently, our group identified a population of very small stem cells (SCs) in murine bone marrow (BM) and other adult organs that express several markers cha...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-010-9143-4
更新日期：2010-06-01 00:00:00
abstract：:Trithorax group (TrxG) proteins play critical roles in transcriptional activation by promoting methylation of histone H3 Lysine 4 (H3K4), but the precise functions of the individual TrxG members during embryonic differentiation are not fully understood. Here we show that Mll2, a TrxG member, is required for proliferat...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-014-9527-y
更新日期：2014-10-01 00:00:00
abstract：:Traumatic injuries are a leading cause of death and disability in both military and civilian populations. Given the complexity and diversity of traumatic injuries, novel and individualized treatment strategies are required to optimize outcomes. Cellular therapies have potential benefit for the treatment of acute or ch...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-020-10086-7
更新日期：2021-01-08 00:00:00
abstract：:There is a need for physiologically relevant assay platforms to provide functionally relevant models of diabetes, to accelerate the discovery of new treatment options and boost developments in drug discovery. In this review, we compare several 3D-strategies that have been used to increase the functional relevance of e...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-017-9783-8
更新日期：2018-04-01 00:00:00
abstract：:Mesenchymal stromal cells (MSCs) constitute a heterogeneous population of stromal cells with immunomodulatory and regenerative properties that support their therapeutic use. MSCs isolated from many tissue sources replicate vigorously in vitro and maintain their main biological properties allowing their widespread clin...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-020-10032-7
更新日期：2020-09-07 00:00:00
abstract：:Bioactive phospholipids, including sphingosine-1-phosphate (S1P), ceramide-1-phosphate (C1P), lysophosphatidylcholine (LPC), and its derivative lysophosphatidic acid (LPA), have emerged as important mediators regulating the trafficking of normal and cancer cells. While the role of S1P in regulating migration of hemato...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-018-9853-6
更新日期：2019-02-01 00:00:00
abstract：:Schwann cells (SC), the glial cells of peripheral nerves, are involved in many diseases including Charcot Marie Tooth and neurofibromatosis, and play a pivotal role in peripheral nerve regeneration. Although it is possible to obtain human SC from nerve biopsies, they are difficult to maintain and expand in culture. He...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-010-9198-2
更新日期：2011-06-01 00:00:00
abstract：:Idiopathic pulmonary fibrosis (IPF) is a devastating disease characterized by obliteration of alveolar architecture, resulting in declining lung function and ultimately death. Pathogenic mechanisms remain unclear but involve a concomitant accumulation of scar tissue together with myofibroblasts activation. Micropartic...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-017-9778-5
更新日期：2018-04-01 00:00:00
abstract：:The molecular profile of human mesenchymal stem cells (MSCs) have emerged as a key factor in defining their identity. Nevertheless, the effect of fetal bovine serum (FBS) batches or origin on MSC molecular signature has been neglected. In this frame, chemical fingerprint of FBS batches from unrelated countries showed ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-018-9822-0
更新日期：2018-12-01 00:00:00
abstract：:An unfortunate emergence of a new virus SARS-CoV-2, causing a disease known as COVID-19, has spread all around the globe and has caused a pandemic. It primarily affects the respiratory tract and lungs in some cases causing severe organ damage and pneumonia due to overwhelming immune responses. Clinical reports show th...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-020-10017-6
更新日期：2020-08-01 00:00:00
abstract：:Neural progenitor cells (NPCs) are multipotent cells that have the potential to produce neurons and glial cells in the neural system. NPCs undergo identity maintenance or differentiation regulated by different kinds of transcription factors. Here we present evidence that ETV5, which is an ETS transcription factor, pro...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-019-09904-4
更新日期：2019-10-01 00:00:00
abstract：:The facile regeneration of undifferentiated human mesenchymal stem cells (hMSCs) from thermoresponsive surfaces facilitates the collection of stem cells avoiding the use of animal derived cell detachment agents commonly used in cell culture. This communication proposes a procedure to fabricate coatings from commercial...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-013-9428-5
更新日期：2013-04-01 00:00:00
abstract：:During the process of development, neural crest cells migrate out from their niche between the newly formed ectoderm and the neural tube. Thereafter, they give rise not only to ectodermal cell types, but also to mesodermal cell types. Cell types with neural crest ancestry consequently comprise a number of specialized ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-011-9340-9
更新日期：2012-09-01 00:00:00
abstract：:The original version of this article unfortunately contained a mistake. In the author group, the correct family name of Dr. Rebeca is "Blázquez" and the correct family name of Dr. Francisco Miguel is "Sánchez-Margallo." ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,已发布勘误
doi：10.1007/s12015-020-09962-z
更新日期：2020-06-01 00:00:00
abstract：:The challenges in limbal stem cell biology largely remain in the process of identification, isolation and expansion of these adult corneal epithelial stem cells of the eye. Due to the absence of specific limbal stem cell markers, identification and isolation of putative limbal stem cells is a complicated task. The sid...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-013-9481-0
更新日期：2014-04-01 00:00:00
abstract：:The combination of cell therapy with growth factors could be a useful approach to treat progressive muscular dystrophies. Here, we demonstrate, for the first time, that IGF-1 considerably enhances the myogenesis of human umbilical cord (UC) mesenchymal stromal cells (MSCs) in vitro and that IGF-1 enhances interaction ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-012-9380-9
更新日期：2013-02-01 00:00:00
