Abstract：
:Mesenchymal stem cells (MSCs) are promising tools for studying the mechanisms of development and for the regeneration of injured tissues. Correct selection of the MSCs source is crucial in order to obtain a more efficient treatment and, in this respect Periosteum-Derived Cells (PDPCs) may represent an interesting alternative to bone marrow MSCs for osteochondral tissue regeneration. In the present study we have isolated and characterized a MSCs population from the periosteum of human adult donors. PDPCs were expanded under specific culture conditions that prevent fibroblast contamination and support the maintenance of their undifferentiated phenotype. We show, for the first time, that PDPCs expresses VEGF receptor (Flt1 and KDR/Flk1) proteins and that they were similar to bone marrow Multipotent Adult Progenitor Cells (MAPCs). Since the latter are able to differentiate into endothelial cells, we tested the possible PDPCs commitment toward an endothelial phenotype in view of bone tissue engineering approaches that takes into account not only bone formation but also vascularization. PDPCs were treated with two different VEGF concentrations for 7 and 15 days and, alternatively, with the supernatant of human primary osteoblasts. Differently from MAPCs our PDPCs were unable to differentiate into endothelial cells after their in vitro VEGF treatment. On the contrary, growth factor stimulation induces PDPCs differentiation toward osteoblasts. We concluded that in PDPCs the presence of VEGF receptors is related to different cross-talk between osteogenesis and angiogenesis that could involve in situ PDPCs recruitment.
journal_name
Stem Cell Rev Repjournal_title
Stem cell reviews and reportsauthors
Ferretti C,Borsari V,Falconi M,Gigante A,Lazzarini R,Fini M,Di Primio R,Mattioli-Belmonte Mdoi
10.1007/s12015-012-9374-7subject
Has Abstractpub_date
2012-09-01 00:00:00pages
882-90issue
3eissn
2629-3269issn
2629-3277journal_volume
8pub_type
杂志文章abstract：:The facile regeneration of undifferentiated human mesenchymal stem cells (hMSCs) from thermoresponsive surfaces facilitates the collection of stem cells avoiding the use of animal derived cell detachment agents commonly used in cell culture. This communication proposes a procedure to fabricate coatings from commercial...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-013-9428-5
更新日期：2013-04-01 00:00:00
abstract：:Epicardial resident stem cells are known to differentiate into cardiomyocytes during cardiac development, amongst other cell types. Whether epicardium-derived progenitor cells (EPDCs) retain this plasticity in the adult heart has been the topic of heated scientific debate. Priming with thymosin beta 4, a peptide which...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-012-9378-3
更新日期：2013-06-01 00:00:00
abstract：:The propensity of induced pluripotent stem (iPS) cells to differentiate into specific lineages may be influenced by a number of factors, including the selection of the somatic cell type used for reprogramming. Herein we report the generation of new iPS cells, which we derived from human articular chondrocytes and from...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-014-9538-8
更新日期：2014-12-01 00:00:00
abstract：:Previous studies demonstrated that induced pluripotent stem (iPS) cells could produce viable mice through tetraploid complementation, which was thought to be the most stringent test for pluripotency. However, these highly pluripotent iPS cells were previously reported to be generated from fibroblasts of embryonic orig...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-010-9160-3
更新日期：2010-09-01 00:00:00
abstract：:Cell-based therapy is a promising approach for many diseases, including ischemic heart disease. Cardiac mesoangioblasts are committed vessel-associated progenitors that can restore to a significant, although partial, extent, heart structure and function in a murine model of myocardial infarction. Low-intensity pulsed ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-015-9608-6
更新日期：2015-12-01 00:00:00
abstract：:Xenografting of normal and transformed mammalian tissues and cells to chick embryos has been performed for almost 100 years. Embryonic stem cells, derived more than 25 years ago from murine, and more than 10 years ago from human blastocysts, have transformed many fields of biological research. There is a growing body ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-010-9161-2
更新日期：2010-09-01 00:00:00
abstract：:Here we present the principles and steps of a protocol that we have recently developed for the differentiation of hES/iPS cells into the authentic human striatal projection medium spiny neurons (MSNs) that die in Huntington's Disease (HD). Authenticity is judged by the convergence of multiple features within individua...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-013-9441-8
更新日期：2013-08-01 00:00:00
abstract：:Mesenchymal stromal cells (MSCs) constitute a heterogeneous population of stromal cells with immunomodulatory and regenerative properties that support their therapeutic use. MSCs isolated from many tissue sources replicate vigorously in vitro and maintain their main biological properties allowing their widespread clin...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-020-10032-7
更新日期：2020-09-07 00:00:00
abstract：:Mesenchymal stem cells (MSCs) represent a population of cells which have the ability to regulate reactivity of T and B lymphocytes by multiple mechanisms. The immunoregulatory activities of MSCs are strictly influenced by the cytokine environment. Here we show that two functionally distinct cytokines, interleukin-4 (I...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-016-9688-y
更新日期：2016-12-01 00:00:00
abstract：:The morphology of several tumors mimics developmentally early tissues; tumors often express early developmental markers characteristic for the germ line lineage. Recently, our group identified a population of very small stem cells (SCs) in murine bone marrow (BM) and other adult organs that express several markers cha...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-010-9143-4
更新日期：2010-06-01 00:00:00
abstract：:The development of pluripotent cells that enable stem cell research (SCR) without destroying human embryos is now a leading priority for science. Public and political controversies associated with human embryonic SCR experienced in the recent past should be alleviated if scientists no longer need to harvest cells from...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-013-9445-4
更新日期：2013-10-01 00:00:00
abstract：:Idiopathic pulmonary fibrosis (IPF) is a devastating disease characterized by obliteration of alveolar architecture, resulting in declining lung function and ultimately death. Pathogenic mechanisms remain unclear but involve a concomitant accumulation of scar tissue together with myofibroblasts activation. Micropartic...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-017-9778-5
更新日期：2018-04-01 00:00:00
abstract：:The model of cancer stem cells in tumor development states that tumors contain a subset of cells that both self renew and give rise to differentiated progeny. Like normal adult tissue stem cells, cancer stem cells are a minority of the whole tumor and are the only cells that are able to maintain tumor growth indefinit...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-008-9048-7
更新日期：2009-03-01 00:00:00
abstract：:The challenges in limbal stem cell biology largely remain in the process of identification, isolation and expansion of these adult corneal epithelial stem cells of the eye. Due to the absence of specific limbal stem cell markers, identification and isolation of putative limbal stem cells is a complicated task. The sid...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-013-9481-0
更新日期：2014-04-01 00:00:00
abstract：:Factors responsible for the rapid proliferative properties of embryonic stem (ES) cells are largely unknown. MicroRNA-221/222 (miR-221/222) regulate proliferation in many somatic cells, however, their roles in proliferation of ES cells are unclear. In this study, E14 mouse ES cells proliferation was determined by tota...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-014-9543-y
更新日期：2015-02-01 00:00:00
abstract：:In this report we review the concept of standardization and propose an exhaustive framework for the proper management of technology on pluripotent stem cells based on studies of global and regional initiatives. We demonstrated detailed observational analysis on global initiatives for the standardization of related tec...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-010-9204-8
更新日期：2011-06-01 00:00:00
abstract：:Reliable and efficient DNA and RNA transfection methods are required when studying the role of individual genes in mouse pluripotent stem cells. However, these cells usually grow in tight clusters and are therefore more difficult to transfect than many other cell lines. We have found that transfection is especially ch...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-016-9673-5
更新日期：2016-10-01 00:00:00
abstract：:The use of mesenchymal stem/stromal cells (MSC) in regenerative medicine often requires MSC to function in environments of high oxidative stress. Human pregnancy is a condition where the mother's tissues, and in particular her circulatory system, are exposed to increased levels of oxidative stress. MSC in the maternal...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-016-9649-5
更新日期：2016-06-01 00:00:00
abstract：:During the process of development, neural crest cells migrate out from their niche between the newly formed ectoderm and the neural tube. Thereafter, they give rise not only to ectodermal cell types, but also to mesodermal cell types. Cell types with neural crest ancestry consequently comprise a number of specialized ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-011-9340-9
更新日期：2012-09-01 00:00:00
abstract：:Brain injury continues to be one of the leading causes of disability worldwide. Despite decades of research, there is currently no pharmacologically effective treatment for preventing neuronal loss and repairing the brain. As a result, novel therapeutic approaches, such as cell-based therapies, are being actively purs...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-013-9484-x
更新日期：2014-04-01 00:00:00
abstract：:Chronic articular cartilage defects are the most common disabling conditions of humans in the western world. The incidence for cartilage defects is increasing with age and the most prominent risk factors are overweight and sports associated overloading. Damage of articular cartilage frequently leads to osteoarthritis ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-012-9405-4
更新日期：2012-12-01 00:00:00
abstract：:The ROCK inhibitor Y-27632 inhibits apoptosis and increases proliferation of frozen-thawed cells. We examined the role of Y-27632 on human umbilical cord Wharton's jelly stem cells (hWJSCs) for (1) thaw-survival (2) proliferation and (3) preservation of stemness and differentiation potential after cryopreservation. hW...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-010-9184-8
更新日期：2010-12-01 00:00:00
abstract：:Angiotensin-converting enzyme 2 (ACE2) plays an important role as a member of the renin-angiotensin-aldosterone system (RAAS) in regulating the conversion of angiotensin II (Ang II) into angiotensin (1-7) (Ang [1-7]). But at the same time, while expressed on the surface of human cells, ACE2 is the entry receptor for S...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-020-10010-z
更新日期：2020-07-20 00:00:00
abstract：:Human embryonic stem cells (hESCs) are especially resistant to several cellular stresses, but the existence and induction of Endoplasmic Reticulum (ER) stress by culture conditions are unknown. Using qPCR, here, we investigated the behavior of the principal sensors of ER stress and their relation with the feeder layer...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-010-9135-4
更新日期：2010-09-01 00:00:00
abstract：:The Networks of Centres of Excellence (NCE) is an innovative model of large-scale research networks that began in Canada in 1989, and has since been adopted by numerous countries around the globe. The Canadian Stem Cell Network (SCN), an NCE that has fostered stem cell research and innovation in Canada, has supported ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-015-9609-5
更新日期：2015-12-01 00:00:00
abstract：:The COVID-19 pandemic has profoundly influenced public health and contributed to global economic divergences of unprecedented dimensions. Due to the high prevalence and mortality rates, it is then expected that the consequence and public health challenges will last for long periods. The rapid global spread of COVID-19...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-020-10108-4
更新日期：2021-01-05 00:00:00
abstract：:The discovery of induced pluripotent stem (iPS) cells in 2006 marked a major breakthrough in regenerative medicine, enabling reversal of terminally differentiated somatic cells into pluripotent stem cells. The embryonic stem (ES) cells-like pluripotency and unlimited self-renewal capability of iPS cells have granted t...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-020-09956-x
更新日期：2020-04-01 00:00:00
abstract：:The absence of effective and satisfactory treatments that contribute to repairing the dopaminergic damage caused by Parkinson's Disease (PD) and the limited recovery capacity of the nervous system are troubling issues and the focus of many research and clinical domains. Recent advances in the treatment of PD through s...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-017-9798-1
更新日期：2018-04-01 00:00:00
abstract：:Oligodendrocyte progenitor cells (OPCs) play a pivotal role in both health and disease within the central nervous system, with oligodendrocytes, arising from resident OPCs, being the main myelinating cell type. Disruption in OPC numbers can lead to various deleterious health defects. Numerous studies have described te...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-017-9742-4
更新日期：2017-08-01 00:00:00
abstract：:Undifferentiated mouse embryonic stem cells (ESCs) possess low numbers of mitochondrial DNA (mtDNA), which encodes key subunits associated with the generation of ATP through oxidative phosphorylation (OXPHOS). As ESCs differentiate, mtDNA copy number is regulated by the nuclear-encoded mtDNA replication factors, which...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-011-9318-7
更新日期：2013-02-01 00:00:00
