Abstract:
:The challenges in limbal stem cell biology largely remain in the process of identification, isolation and expansion of these adult corneal epithelial stem cells of the eye. Due to the absence of specific limbal stem cell markers, identification and isolation of putative limbal stem cells is a complicated task. The side population assay is an isolation method that utilises the ability of stem cells to efflux the DNA-binding dye Hoechst 33342 (or other vital dyes) combined with dual wavelength flow cytometry and is a valuable strategy to enrich for limbal stem cells. This assay has been used to successfully identify stem/ progenitor cell populations in a variety of tissues and cell lines. Here we optimise this assay to identify SP cell populations in both primary human limbal epithelial cultures and in an established human corneal epithelial cell line. The limbal SP fraction showed higher expression of ATP-binding cassette sub-family G member 2 (ABCG2), ΔNp63--a common limbal stem cell marker and the stem cell marker Sox2 compared to non-SP cells (NSP).
journal_name
Stem Cell Rev Repjournal_title
Stem cell reviews and reportsauthors
Shaharuddin B,Harvey I,Ahmad S,Ali S,Meeson Adoi
10.1007/s12015-013-9481-0subject
Has Abstractpub_date
2014-04-01 00:00:00pages
240-50issue
2eissn
2629-3269issn
2629-3277journal_volume
10pub_type
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