Abstract:
:Factors responsible for the rapid proliferative properties of embryonic stem (ES) cells are largely unknown. MicroRNA-221/222 (miR-221/222) regulate proliferation in many somatic cells, however, their roles in proliferation of ES cells are unclear. In this study, E14 mouse ES cells proliferation was determined by total cell counting, Cell Counting Kit (CCK-8), size of colonies and cell cycle analysis, while apoptosis and necrosis using Annexin V and propidium iodide staining. miR-221 inhibitor decreased proliferation of ES cells without inducing apoptosis and necrosis. miR-221 mimic, miR-222 mimic and miR-222 inhibitor did not affect ES cells proliferation. The expression level of miR-221 remained unchanged upon embryoid body (EB) formation. ES cells with miR-221 inhibition maintained an undifferentiated state, as indicated by unchanged alkaline phosphatase enzyme activity and Sox2, Nanong, and Oct4 expressions. P57 was post-transcriptionally regulated by miR-221 in ES cells. P57 knockdown completely abolished the inhibition effects of ES cells proliferation observed in miR-221 reduction, further indicating that miR-221 inhibition is likely to mediate its antiproliferative effects via P57 expression. To exclude that the function of miR-221 in ES cells is E14 specific, the effects of miR-221 mimic and inhibitor in size of colonies and cell cycle of R1 mouse ES cells were also determined and similar effects in inhibiting proliferation were achieved with miR-221 inhibition. Therefore, miR-221 is required for mouse ES cells proliferation via P57 targeting. This study indicates that miR-221 is among the regulators that control ES cells proliferation and might be used to influence the fate of ES cells.
journal_name
Stem Cell Rev Repjournal_title
Stem cell reviews and reportsauthors
Li J,Bei Y,Liu Q,Lv D,Xu T,He Y,Chen P,Xiao Jdoi
10.1007/s12015-014-9543-ysubject
Has Abstractpub_date
2015-02-01 00:00:00pages
39-49issue
1eissn
2629-3269issn
2629-3277journal_volume
11pub_type
杂志文章abstract::Stem cells in the endometrium and myometrium possess an immense regenerative potential which is necessary to maintain the menstrual cycle and support pregnancy. These cells, as well as bone marrow stem cells, have also been implicated in the development of common benign gynecological disorders including leiomyomas, en...
journal_title:Stem cell reviews and reports
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journal_title:Stem cell reviews and reports
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journal_title:Stem cell reviews and reports
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journal_title:Stem cell reviews and reports
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journal_title:Stem cell reviews and reports
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journal_title:Stem cell reviews and reports
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journal_title:Stem cell reviews and reports
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journal_title:Stem cell reviews and reports
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journal_title:Stem cell reviews and reports
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abstract::The discovery of induced pluripotent stem (iPS) cells in 2006 marked a major breakthrough in regenerative medicine, enabling reversal of terminally differentiated somatic cells into pluripotent stem cells. The embryonic stem (ES) cells-like pluripotency and unlimited self-renewal capability of iPS cells have granted t...
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journal_title:Stem cell reviews and reports
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