Abstract：
:Although bone marrow-derived mononuclear cells (BMNC) have been extensively used in cell therapy for cardiac diseases, little mechanistic information is available to support reports of their efficacy. To address this shortcoming, we compared structural and functional recovery and associated global gene expression profiles in post-ischaemic myocardium treated with BMNC transplantation. BMNC suspensions were injected into cardiac scar tissue 10 days after experimental myocardial infarction. Six weeks later, mice undergoing BMNC therapy were found to have normalized antibody repertoire and improved cardiac performance measured by ECG, treadmill exercise time and echocardiography. After functional testing, gene expression profiles in cardiac tissue were evaluated using high-density oligonucleotide arrays. Expression of more than 18% of the 11981 quantified unigenes was significantly altered in the infarcted hearts. BMNC therapy restored expression of 2099 (96.2%) of the genes that were altered by infarction but led to altered expression of 286 other genes, considered to be a side effect of the treatment. Transcriptional therapeutic efficacy, a metric calculated using a formula that incorporates both recovery and side effect of treatment, was 73%. In conclusion, our results confirm a beneficial role for bone marrow-derived cell therapy and provide new information on molecular mechanisms operating after BMNC transplantation on post ischemic heart failure in mice.
journal_name
Stem Cell Rev Repjournal_title
Stem cell reviews and reportsauthors
Lachtermacher S,Esporcatte BL,Fortes Fda S,Rocha NN,Montalvão F,Costa PC,Belem L,Rabischoffisky A,Faria Neto HC,Vasconcellos R,Iacobas DA,Iacobas S,Spray DC,Thomas NM,Goldenberg RC,de Carvalho ACdoi
10.1007/s12015-011-9282-2subject
Has Abstractpub_date
2012-03-01 00:00:00pages
251-61issue
1eissn
2629-3269issn
2629-3277journal_volume
8pub_type
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