Abstract:
BACKGROUND:Mesenchymal stem cells derived from the chorionic villi of human term placenta (pMSCs) have drawn considerable interest because of their multipotent differentiation potential and their immunomodulatory capacity. These properties are the foundation for their clinical application in the fields of stem cell transplantation and regenerative medicine. Previously, we showed that pMSCs induce an anti-inflammatory phenotype in human macrophages. In this study, we determined whether pMSCs modify the differentiation and maturation of human monocytes into dendritic cells (DCs). The consequences on dendritic function and on T cell proliferation were also investigated. METHODS:Interleukin-4 (IL-4) and granulocyte-macrophage colony stimulating factor (GM-CSF) were used to stimulate the differentiation of monocytes into immature dendritic cells (iDCs), which were subsequently co-cultured with pMSCs. Lipopolysaccharide (LPS) was used to induce maturation of iDCs into mature dendritic cells (mDCs). Flow cytometry and enzyme-linked immunosorbent assays (ELISA) were used to quantify the effect pMSC co-culturing on DC differentiation using CD1a, a distinctive marker of DCs, as well as other molecules important in the immune functions of DCs. The phagocytic activity of iDCs co-cultured with pMSCs, and the effects of iDCs and mDC stimulation on T cell proliferation, were also investigated. RESULTS:Monocyte differentiation into iDCs was inhibited when co-cultured with pMSCs and maturation of iDCs by LPS treatment was also prevented in the presence of pMSCs as demonstrated by reduced expression of CD1a and CD83, respectively. The inhibitory effect of pMSCs on iDC differentiation was dose dependent. In addition, pMSC co-culture with iDCs and mDCs resulted in both phenotypic and functional changes as shown by reduced expression of costimulatory molecules (CD40, CD80, CD83 and CD86) and reduced capacity to stimulate CD4(+) T cell proliferation. In addition, pMSC co-culture increased the surface expression of major histocompatibility complex (MHC-II) molecules on iDCs but decreased MHC-II expression on mDCs. Moreover, pMSC co-culture with iDCs or mDCs increased the expression of immunosuppressive molecules [B7H3, B7H4, CD273, CD274 and indoleamine-pyrrole 2,3-dioxygenase (IDO). Additionally, the secretion of IL-12 and IL-23 by iDCs and mDCs co-cultured with pMSCs was decreased. Furthermore, pMSC co-culture with mDCs decreased the secretion of IL-12 and INF-γ whilst increasing the secretion of IL-10 in a T cell proliferation experiment. Finally, pMSC co-culture with iDCs induced the phagocytic activity of iDCs. CONCLUSIONS:We have shown that pMSCs have an inhibitory effect on the differentiation, maturation and function of DCs, as well as on the proliferation of T cells, suggesting that pMSCs can control the immune responses at multiple levels.
journal_name
Stem Cell Rev Repjournal_title
Stem cell reviews and reportsauthors
Abomaray FM,Al Jumah MA,Kalionis B,AlAskar AS,Al Harthy S,Jawdat D,Al Khaldi A,Alkushi A,Knawy BA,Abumaree MHdoi
10.1007/s12015-014-9562-8subject
Has Abstractpub_date
2015-06-01 00:00:00pages
423-41issue
3eissn
2629-3269issn
2629-3277journal_volume
11pub_type
杂志文章abstract::Surface topography of Titanium (Ti) dental implants strongly influences osseointegration. In the present work, we have analyzed the influence of two Ti implant surfaces characterized by similar microtopography but different nanotopography and chemistry on the osteoblastic phenotype of Dental Pulp Stem Cells (DPSCs). T...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-016-9669-1
更新日期:2016-10-01 00:00:00
abstract::Converting adult cells from one cell type to another is a particularly interesting idea for regenerative medicine. Terminally differentiated cells can be induced to de-differentiate in vitro to become multipotent progenitors. In mammals these changes do not occur naturally, however exposing differentiated adult cells ...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-014-9521-4
更新日期:2014-10-01 00:00:00
abstract::Nitric oxide (NO) is a gaseous free radical molecule involved in several biological processes related to inflammation, tissue damage, and infections. Based on reports that NO inhibits migration of granulocytes and monocytes, we became interested in the role of inducible NO synthetase (iNOS) in pharmacological mobiliza...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-016-9693-1
更新日期:2017-02-01 00:00:00
abstract::The intestine integrates the function of digestion, absorption, and barrier, which is easily damaged by the external factors upon ageing. The intestinal stem cells (ISCs) exist at the intestinal crypt base and play an indispensable role in intestinal homeostasis and regeneration. The intestine ageing contributes to ma...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-020-10073-y
更新日期:2020-11-17 00:00:00
abstract::Hematopoietic stem cells (HSCs) from umbilical cord blood have been successfully used to treat blood disorders but one major hurdle is the relatively low cell dose available. Double cord blood unit transplantation results in elevated engraftment failure because one unit predominates over the other. Various approaches ...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-013-9426-7
更新日期:2013-04-01 00:00:00
abstract::The inward rectifier potassium current (IK1) is generally thought to suppress cardiac automaticity by hyperpolarizing membrane potential (MP). We recently observed that IK1 could promote the spontaneously-firing automaticity induced by upregulation of pacemaker funny current (If) in adult ventricular cardiomyocytes (C...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-017-9745-1
更新日期:2017-10-01 00:00:00
abstract::The facile regeneration of undifferentiated human mesenchymal stem cells (hMSCs) from thermoresponsive surfaces facilitates the collection of stem cells avoiding the use of animal derived cell detachment agents commonly used in cell culture. This communication proposes a procedure to fabricate coatings from commercial...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-013-9428-5
更新日期:2013-04-01 00:00:00
abstract::Zinc-finger nucleases (ZFNs) are sequence-specific genome engineering tools with great potential for the development of gene therapies. The achievement of permanent cures through gene therapy requires targeting of stem cells but the effects and/or side effects of ZFN treatment on adult stem cell potency are largely un...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-011-9313-z
更新日期:2012-06-01 00:00:00
abstract::Trithorax group (TrxG) proteins play critical roles in transcriptional activation by promoting methylation of histone H3 Lysine 4 (H3K4), but the precise functions of the individual TrxG members during embryonic differentiation are not fully understood. Here we show that Mll2, a TrxG member, is required for proliferat...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-014-9527-y
更新日期:2014-10-01 00:00:00
abstract::Neural progenitor cells (NPCs) are multipotent cells that have the potential to produce neurons and glial cells in the neural system. NPCs undergo identity maintenance or differentiation regulated by different kinds of transcription factors. Here we present evidence that ETV5, which is an ETS transcription factor, pro...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-019-09904-4
更新日期:2019-10-01 00:00:00
abstract::Chronic articular cartilage defects are the most common disabling conditions of humans in the western world. The incidence for cartilage defects is increasing with age and the most prominent risk factors are overweight and sports associated overloading. Damage of articular cartilage frequently leads to osteoarthritis ...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-012-9405-4
更新日期:2012-12-01 00:00:00
abstract::Mesenchymal stromal cells (MSCs) constitute a heterogeneous population of stromal cells with immunomodulatory and regenerative properties that support their therapeutic use. MSCs isolated from many tissue sources replicate vigorously in vitro and maintain their main biological properties allowing their widespread clin...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-020-10032-7
更新日期:2020-09-07 00:00:00
abstract::Understanding the genetic and molecular mechanisms of ovarian cancer has been the focus of research efforts working toward the greater goal of improving cancer therapy for patients with residual disease after initial treatment with conventional surgery and neoadjuvant chemotherapy. The focus of this review will be cen...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-011-9344-5
更新日期:2012-09-01 00:00:00
abstract::The morphology of several tumors mimics developmentally early tissues; tumors often express early developmental markers characteristic for the germ line lineage. Recently, our group identified a population of very small stem cells (SCs) in murine bone marrow (BM) and other adult organs that express several markers cha...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-010-9143-4
更新日期:2010-06-01 00:00:00
abstract::The COVID-19 pandemic has profoundly influenced public health and contributed to global economic divergences of unprecedented dimensions. Due to the high prevalence and mortality rates, it is then expected that the consequence and public health challenges will last for long periods. The rapid global spread of COVID-19...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-020-10108-4
更新日期:2021-01-05 00:00:00
abstract::We previously showed the emergence of predominantly non-fused murine cells co-expressing cardiac and stromal determinants in co-cultures of murine mesenchymal stromal cells (MSCs) and rat embryonic cardiomyocytes. To determine whether a similar phenotype is detectable in vivo in ischemic myocardium, we infused green f...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-013-9483-y
更新日期:2014-04-01 00:00:00
abstract::Xenografting of normal and transformed mammalian tissues and cells to chick embryos has been performed for almost 100 years. Embryonic stem cells, derived more than 25 years ago from murine, and more than 10 years ago from human blastocysts, have transformed many fields of biological research. There is a growing body ...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-010-9161-2
更新日期:2010-09-01 00:00:00
abstract::Metastasis is the worst prognosis predictor in the clinical course of cancer development. Features of metastatic cancer cells include migratory ability, low degree of differentiation, self-renewal and proliferation potentials, as well as resistance to therapies. Metastatic cells do not present all of the necessary cha...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-019-09936-w
更新日期:2020-04-01 00:00:00
abstract::Stem cell research has generated considerable attention for its potential to remediate many disorders of the central nervous system including neurodevelopmental disorders such as autism spectrum disorder (ASD) and cerebral palsy (CP) that place a high burden on individual children, families and society. Here we charac...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-015-9627-3
更新日期:2016-02-01 00:00:00
abstract::The vertebrate early stage embryo is consisting of the three primary germ layers ectoderm, mesoderm and endoderm, from which all organ tissues are developed. During early embryonic development, mesodermal cells become sequentially determined to more precisely defined cell types including muscle, heart, vasculature, bl...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-011-9263-5
更新日期:2012-03-01 00:00:00
abstract::Various strategies have been published enabling cardiomyocyte differentiation of human induced pluripotent stem (iPS) cells. However the complex nature of signaling pathways involved as well as line-to-line variability compromises the application of a particular protocol to robustly obtain cardiomyocytes from multiple...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-014-9564-6
更新日期:2015-08-01 00:00:00
abstract::The reprogramming of adult somatic cells into an embryonic stem cell (ESC) state by various means has opened a new chapter in basic and applied life science. While this technology will create great opportunities for regenerative medicine, the more immediate impact is likely to be found in human disease modeling and dr...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-011-9342-7
更新日期:2013-08-01 00:00:00
abstract::Derivation of human embryonic stem cell (hES) lines in 1998 was not only a major technological breakthrough in the field of regenerative medicine; it also triggered a passionate debate about the ethical issues associated with the utilization of human embryos for derivation of hESC lines. Successful derivation of induc...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-017-9766-9
更新日期:2017-12-01 00:00:00
abstract::There is a need for physiologically relevant assay platforms to provide functionally relevant models of diabetes, to accelerate the discovery of new treatment options and boost developments in drug discovery. In this review, we compare several 3D-strategies that have been used to increase the functional relevance of e...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-017-9783-8
更新日期:2018-04-01 00:00:00
abstract::Idiopathic pulmonary fibrosis (IPF) is a devastating disease characterized by obliteration of alveolar architecture, resulting in declining lung function and ultimately death. Pathogenic mechanisms remain unclear but involve a concomitant accumulation of scar tissue together with myofibroblasts activation. Micropartic...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-017-9778-5
更新日期:2018-04-01 00:00:00
abstract::The model of cancer stem cells in tumor development states that tumors contain a subset of cells that both self renew and give rise to differentiated progeny. Like normal adult tissue stem cells, cancer stem cells are a minority of the whole tumor and are the only cells that are able to maintain tumor growth indefinit...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-008-9048-7
更新日期:2009-03-01 00:00:00
abstract::Factors responsible for the rapid proliferative properties of embryonic stem (ES) cells are largely unknown. MicroRNA-221/222 (miR-221/222) regulate proliferation in many somatic cells, however, their roles in proliferation of ES cells are unclear. In this study, E14 mouse ES cells proliferation was determined by tota...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-014-9543-y
更新日期:2015-02-01 00:00:00
abstract::In this report we review the concept of standardization and propose an exhaustive framework for the proper management of technology on pluripotent stem cells based on studies of global and regional initiatives. We demonstrated detailed observational analysis on global initiatives for the standardization of related tec...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-010-9204-8
更新日期:2011-06-01 00:00:00
abstract::Undifferentiated mouse embryonic stem cells (ESCs) possess low numbers of mitochondrial DNA (mtDNA), which encodes key subunits associated with the generation of ATP through oxidative phosphorylation (OXPHOS). As ESCs differentiate, mtDNA copy number is regulated by the nuclear-encoded mtDNA replication factors, which...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-011-9318-7
更新日期:2013-02-01 00:00:00
abstract::Schwann cells (SC), the glial cells of peripheral nerves, are involved in many diseases including Charcot Marie Tooth and neurofibromatosis, and play a pivotal role in peripheral nerve regeneration. Although it is possible to obtain human SC from nerve biopsies, they are difficult to maintain and expand in culture. He...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-010-9198-2
更新日期:2011-06-01 00:00:00