Abstract:
:Various strategies have been published enabling cardiomyocyte differentiation of human induced pluripotent stem (iPS) cells. However the complex nature of signaling pathways involved as well as line-to-line variability compromises the application of a particular protocol to robustly obtain cardiomyocytes from multiple iPS lines. Hence it is necessary to identify optimized protocols with alternative combinations of specific growth factors and small molecules to enhance the robustness of cardiac differentiation. Here we focus on systematic modulation of BMP and WNT signaling to enhance cardiac differentiation. Moreover, we improve the efficacy of cardiac differentiation by enrichment via lactate. Using our protocol we show efficient derivation of cardiomyocytes from multiple human iPS lines. In particular we demonstrate cardiomyocyte differentiation within 15 days with an efficiency of up to 95 % as judged by flow cytometry staining against cardiac troponin T. Cardiomyocytes derived were functionally validated by alpha-actinin staining, transmission electron microscopy as well as electrophysiological analysis. We expect our protocol to provide a robust basis for scale-up production of functional iPS cell-derived cardiomyocytes that can be used for cell replacement therapy and disease modeling.
journal_name
Stem Cell Rev Repjournal_title
Stem cell reviews and reportsauthors
Kadari A,Mekala S,Wagner N,Malan D,Köth J,Doll K,Stappert L,Eckert D,Peitz M,Matthes J,Sasse P,Herzig S,Brüstle O,Ergün S,Edenhofer Fdoi
10.1007/s12015-014-9564-6subject
Has Abstractpub_date
2015-08-01 00:00:00pages
560-9issue
4eissn
2629-3269issn
2629-3277journal_volume
11pub_type
杂志文章abstract::Adipogenesis is a complex process whereby the multipotent adipose-derived stem cell is converted to a preadipocyte before terminal differentiation into the mature adipocyte. Preadipocytes are present throughout adult life, exhibit adipose fat depot specificity, and differentiate and proliferate from distinct progenito...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-017-9774-9
更新日期:2018-02-01 00:00:00
abstract:BACKGROUND:Mesenchymal stem cells derived from the chorionic villi of human term placenta (pMSCs) have drawn considerable interest because of their multipotent differentiation potential and their immunomodulatory capacity. These properties are the foundation for their clinical application in the fields of stem cell tra...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-014-9562-8
更新日期:2015-06-01 00:00:00
abstract::Oligodendrocyte progenitor cells (OPCs) play a pivotal role in both health and disease within the central nervous system, with oligodendrocytes, arising from resident OPCs, being the main myelinating cell type. Disruption in OPC numbers can lead to various deleterious health defects. Numerous studies have described te...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-017-9742-4
更新日期:2017-08-01 00:00:00
abstract::Since its early days, stem cell research, particularly human embryonic stem cell research, has been the focus of intense social debate, and the question of the moral status of the embryo has been a central issue in the controversy. Despite this friction, and while it has yet to obtain widespread success in clinical ap...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-009-9062-4
更新日期:2009-06-01 00:00:00
abstract::Osteoarthritis (OA) remains incurable in humans or horses and mesenchymal stromal/stem cells (MSCs) represent an attractive solution for producing a neocartilage substitute. However, the best MSC source still needs to be identified. This study compared the chondrogenic potential of equine MSCs derived from bone marrow...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-019-09914-2
更新日期:2020-02-01 00:00:00
abstract::Recent advances on human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have brought us closer to the realization of their clinical potential. Nonetheless, tissue engineering and regenerative medicine applications will require the generation of ...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-014-9544-x
更新日期:2015-02-01 00:00:00
abstract::The practice of travelling abroad to receive unproven and unregulated stem cell treatments has become an increasingly problematic global phenomenon known as 'stem cell tourism'. In this paper, we examine representations of nine major clinics and providers of such treatments on the microblogging network Twitter. We col...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-014-9534-z
更新日期:2014-12-01 00:00:00
abstract::The morphology of several tumors mimics developmentally early tissues; tumors often express early developmental markers characteristic for the germ line lineage. Recently, our group identified a population of very small stem cells (SCs) in murine bone marrow (BM) and other adult organs that express several markers cha...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-010-9143-4
更新日期:2010-06-01 00:00:00
abstract::The reprogramming of adult somatic cells into an embryonic stem cell (ESC) state by various means has opened a new chapter in basic and applied life science. While this technology will create great opportunities for regenerative medicine, the more immediate impact is likely to be found in human disease modeling and dr...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-011-9342-7
更新日期:2013-08-01 00:00:00
abstract::Schwann cells (SC), the glial cells of peripheral nerves, are involved in many diseases including Charcot Marie Tooth and neurofibromatosis, and play a pivotal role in peripheral nerve regeneration. Although it is possible to obtain human SC from nerve biopsies, they are difficult to maintain and expand in culture. He...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-010-9198-2
更新日期:2011-06-01 00:00:00
abstract::Human pluripotent stem cells (PSCs) are critical in vitro tools for understanding mechanisms that regulate lineage differentiation in the human embryo as well as a potentially unlimited supply of stem cells for regenerative medicine. Pluripotent human and mouse embryonic stem cells (ESCs) derived from the inner cell m...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-013-9490-z
更新日期:2014-04-01 00:00:00
abstract::The majority of patients survive an acute myocardial infarction (AMI). Their outcome is negatively influenced by post-AMI events, such as loss of viable cardiomyocytes due to a post-AMI inflammatory response, eventually resulting in heart failure and/or death. Recent pre-clinical animal studies indicate that mesenchym...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-014-9502-7
更新日期:2014-06-01 00:00:00
abstract::Mobilization of stem cells from bone marrow (BM) into peripheral blood (PB) in response to tissue or organ injury, infections, strenuous exercise, or mobilization-inducing drugs is as we postulated result of a "sterile inflammation" in the BM microenvironment that triggers activation of the Complement Cascade (ComC). ...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-019-09890-7
更新日期:2019-06-01 00:00:00
abstract::Derivation of human embryonic stem cell (hES) lines in 1998 was not only a major technological breakthrough in the field of regenerative medicine; it also triggered a passionate debate about the ethical issues associated with the utilization of human embryos for derivation of hESC lines. Successful derivation of induc...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-017-9766-9
更新日期:2017-12-01 00:00:00
abstract::Reliable and efficient DNA and RNA transfection methods are required when studying the role of individual genes in mouse pluripotent stem cells. However, these cells usually grow in tight clusters and are therefore more difficult to transfect than many other cell lines. We have found that transfection is especially ch...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-016-9673-5
更新日期:2016-10-01 00:00:00
abstract::The COVID-19 pandemic has profoundly influenced public health and contributed to global economic divergences of unprecedented dimensions. Due to the high prevalence and mortality rates, it is then expected that the consequence and public health challenges will last for long periods. The rapid global spread of COVID-19...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-020-10108-4
更新日期:2021-01-05 00:00:00
abstract::Endothelial progenitors are a population of cells with the inherent capacity to differentiate into mature endothelial cells and proangiogenic paracrine action. These characteristics have led to extensive studies being performed and tested in the treatment of tissue ischemia. The natural course of diabetes mellitus (DM...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-018-9863-4
更新日期:2019-04-01 00:00:00
abstract::The propensity of induced pluripotent stem (iPS) cells to differentiate into specific lineages may be influenced by a number of factors, including the selection of the somatic cell type used for reprogramming. Herein we report the generation of new iPS cells, which we derived from human articular chondrocytes and from...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-014-9538-8
更新日期:2014-12-01 00:00:00
abstract::Brain injury continues to be one of the leading causes of disability worldwide. Despite decades of research, there is currently no pharmacologically effective treatment for preventing neuronal loss and repairing the brain. As a result, novel therapeutic approaches, such as cell-based therapies, are being actively purs...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-013-9484-x
更新日期:2014-04-01 00:00:00
abstract::In this report we review the concept of standardization and propose an exhaustive framework for the proper management of technology on pluripotent stem cells based on studies of global and regional initiatives. We demonstrated detailed observational analysis on global initiatives for the standardization of related tec...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-010-9204-8
更新日期:2011-06-01 00:00:00
abstract::Understanding the healthy and diseased state of skin is important in many areas of basic and applied research. Although the field of skin tissue engineering has advanced greatly over the last years, current in vitro skin models still do not mimic the complexity of the human skin. Skin-on-chip and induced pluripotent s...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-017-9737-1
更新日期:2017-06-01 00:00:00
abstract::During the process of development, neural crest cells migrate out from their niche between the newly formed ectoderm and the neural tube. Thereafter, they give rise not only to ectodermal cell types, but also to mesodermal cell types. Cell types with neural crest ancestry consequently comprise a number of specialized ...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-011-9340-9
更新日期:2012-09-01 00:00:00
abstract::Few surface markers are available to monitor lineage differentiation during chondrogenesis. Recently, delta-like1/fetal antigen1 (dlk1/FA1), a transmembrane protein of the Notch/Delta/Serrata family, was shown to be essential for inducing early chondrogenesis. Thus, we investigated the possible use of dlk1/FA1 as a no...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-009-9099-4
更新日期:2009-12-01 00:00:00
abstract::Stem cell research has generated considerable attention for its potential to remediate many disorders of the central nervous system including neurodevelopmental disorders such as autism spectrum disorder (ASD) and cerebral palsy (CP) that place a high burden on individual children, families and society. Here we charac...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-015-9627-3
更新日期:2016-02-01 00:00:00
abstract::Metastasis is the worst prognosis predictor in the clinical course of cancer development. Features of metastatic cancer cells include migratory ability, low degree of differentiation, self-renewal and proliferation potentials, as well as resistance to therapies. Metastatic cells do not present all of the necessary cha...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-019-09936-w
更新日期:2020-04-01 00:00:00
abstract:PURPOSE:Evaluation of potential tropic effects of vascular endothelial growth factor (VEGF) on the incorporation and differentiation of bone-marrow-derived stem cells (BMSCs) in a murine model of anterior ischemic optic neuropathy (AION). METHODS:In the first approach, small-sized subset of BMCs were isolated from GFP...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-012-9396-1
更新日期:2012-12-01 00:00:00
abstract::Cell-based therapy is a promising approach for many diseases, including ischemic heart disease. Cardiac mesoangioblasts are committed vessel-associated progenitors that can restore to a significant, although partial, extent, heart structure and function in a murine model of myocardial infarction. Low-intensity pulsed ...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-015-9608-6
更新日期:2015-12-01 00:00:00
abstract::Human embryonic stem cells (hESCs) can be propagated indefinitely in vitro in an undifferentiated pluripotent state, can differentiate into derivatives of all three germ layers and are of considerable interest for applications in regenerative medicine. Clinical application of hESCs, however, requires reliable protocol...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-011-9230-1
更新日期:2011-09-01 00:00:00
abstract::Epicardial resident stem cells are known to differentiate into cardiomyocytes during cardiac development, amongst other cell types. Whether epicardium-derived progenitor cells (EPDCs) retain this plasticity in the adult heart has been the topic of heated scientific debate. Priming with thymosin beta 4, a peptide which...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-012-9378-3
更新日期:2013-06-01 00:00:00
abstract::Mesenchymal stromal cells (MSCs) constitute a heterogeneous population of stromal cells with immunomodulatory and regenerative properties that support their therapeutic use. MSCs isolated from many tissue sources replicate vigorously in vitro and maintain their main biological properties allowing their widespread clin...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-020-10032-7
更新日期:2020-09-07 00:00:00