Abstract:
:The ROCK inhibitor Y-27632 inhibits apoptosis and increases proliferation of frozen-thawed cells. We examined the role of Y-27632 on human umbilical cord Wharton's jelly stem cells (hWJSCs) for (1) thaw-survival (2) proliferation and (3) preservation of stemness and differentiation potential after cryopreservation. hWJSCs were allotted to 4 groups [Gp I: Untreated hWJSC controls; Gp II: Pretreatment with Y-27632 (10 μM) for 24 h before freezing; Gp III: Y-27632 (10 μM) in freezing medium and Gp IV: Pretreatment with Y-27632 (10 μM) for 24 h and inclusion in freezing medium]. All groups were frozen using a rapid freezing method and stored at -196°C in liquid nitrogen for 90 days before evaluation for apoptosis, cell proliferation, stemness and differentiation. After thawing, Groups II, III and IV showed improved cell attachment, increased thaw-survival (live/dead cell counts) and increased cell proliferation (Trypan blue and MTT assay) compared to controls. CD marker stemness profiles, morphology and normal karyotypes were maintained in the treatment groups after thawing and there was no obvious evidence of apoptosis (Annexin V-FITC and TUNEL assays). After thawing, qRT-PCR demonstrated up-regulation of the anti-apoptotic BCL2 gene and down-regulation of the pro-apoptotic BAX gene and cell cycle regulators (P53 and P21) in the treatment groups. Treated frozen-thawed hWJSCs from all groups differentiated into a neuronal phenotype (neuronal morphology and expression of GFAP, β-3 tubulin and SOX2). Increased thaw-survival and retention of stemness and differentiation potential in hWJSCs following cryopreservation is useful for their storage in cord blood banks for future regenerative medicine purposes.
journal_name
Stem Cell Rev Repjournal_title
Stem cell reviews and reportsauthors
Gauthaman K,Fong CY,Subramanian A,Biswas A,Bongso Adoi
10.1007/s12015-010-9184-8subject
Has Abstractpub_date
2010-12-01 00:00:00pages
665-76issue
4eissn
2629-3269issn
2629-3277journal_volume
6pub_type
杂志文章abstract::Trithorax group (TrxG) proteins play critical roles in transcriptional activation by promoting methylation of histone H3 Lysine 4 (H3K4), but the precise functions of the individual TrxG members during embryonic differentiation are not fully understood. Here we show that Mll2, a TrxG member, is required for proliferat...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-014-9527-y
更新日期:2014-10-01 00:00:00
abstract::In this report we review the concept of standardization and propose an exhaustive framework for the proper management of technology on pluripotent stem cells based on studies of global and regional initiatives. We demonstrated detailed observational analysis on global initiatives for the standardization of related tec...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-010-9204-8
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-013-9483-y
更新日期:2014-04-01 00:00:00
abstract::The absence of effective and satisfactory treatments that contribute to repairing the dopaminergic damage caused by Parkinson's Disease (PD) and the limited recovery capacity of the nervous system are troubling issues and the focus of many research and clinical domains. Recent advances in the treatment of PD through s...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-017-9798-1
更新日期:2018-04-01 00:00:00
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-019-09943-x
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abstract::The interest in the use of stem cells as a source for therapy has increased dramatically over the last decades. Different stem cell types have been tested in both in vitro and in vivo models, because of their properties such as differentiation potential, trophic effects and immune modulatory properties. To further opt...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-014-9575-3
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journal_title:Stem cell reviews and reports
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
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更新日期:2012-12-01 00:00:00
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journal_title:Stem cell reviews and reports
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章
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更新日期:2018-04-01 00:00:00
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章,已发布勘误
doi:10.1007/s12015-020-09962-z
更新日期:2020-06-01 00:00:00
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-020-10032-7
更新日期:2020-09-07 00:00:00
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-014-9534-z
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-017-9766-9
更新日期:2017-12-01 00:00:00
abstract::Pre-clinical studies have supported the use of mesenchymal stem cells (MSC) to treat highly prevalent neurodegenerative diseases such as Parkinson's disease (PD) but preliminary trials have reported controversial results. In a rat model of PD induced by MPTP neurotoxin, we first observed a significant bilateral preser...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-011-9256-4
更新日期:2011-11-01 00:00:00
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章
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更新日期:2014-04-01 00:00:00
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-020-10052-3
更新日期:2020-12-01 00:00:00
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-011-9340-9
更新日期:2012-09-01 00:00:00
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-019-09904-4
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journal_title:Stem cell reviews and reports
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journal_title:Stem cell reviews and reports
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journal_title:Stem cell reviews and reports
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
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更新日期:2014-10-01 00:00:00
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-016-9693-1
更新日期:2017-02-01 00:00:00
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
doi:10.1007/s12015-011-9263-5
更新日期:2012-03-01 00:00:00
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journal_title:Stem cell reviews and reports
pub_type: 杂志文章,评审
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更新日期:2011-06-01 00:00:00
abstract::Cell-based therapy is a promising approach for many diseases, including ischemic heart disease. Cardiac mesoangioblasts are committed vessel-associated progenitors that can restore to a significant, although partial, extent, heart structure and function in a murine model of myocardial infarction. Low-intensity pulsed ...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-015-9608-6
更新日期:2015-12-01 00:00:00
abstract::Cirrhosis is associated with dysregulated immune cell activation and immune dysfunction. These conditions modify gut flora, facilitate bacterial translocation, and increase susceptibility to bacterial peritonitis and consequent systemic infections by dramatically affecting long-term patient survival. Human amnion-deri...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-020-10104-8
更新日期:2021-01-03 00:00:00
abstract::Please note the following errors in the original version. ...
journal_title:Stem cell reviews and reports
pub_type: 已发布勘误
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更新日期:2019-02-01 00:00:00
abstract::Converting adult cells from one cell type to another is a particularly interesting idea for regenerative medicine. Terminally differentiated cells can be induced to de-differentiate in vitro to become multipotent progenitors. In mammals these changes do not occur naturally, however exposing differentiated adult cells ...
journal_title:Stem cell reviews and reports
pub_type: 杂志文章
doi:10.1007/s12015-014-9521-4
更新日期:2014-10-01 00:00:00