Abstract：
BACKGROUND:Bone marrow-derived mononuclear cells (BM-MNC) consist of a heterogeneous mix of mesenchymal stem cells (MSC), hematopoietic progenitor cells (HPC), endothelial progenitor cells (EPC), monocytes, lymphocytes and pluripotent stem cells. Whereas the importance of MSC and EPC has been well documented in bone healing and regeneration studies, the role of pluripotent stem cells is still poorly understood. In the present study we evaluated if and how Very Small Embryonic Like cells (VSEL), isolated from rat BM-MNC, contribute to bone healing. METHODS:Large bone defects were made in the femurs of 38 Sprague Dawley female rats and treated with β-TCP scaffold granules seeded with male VSEL; BM-MNC, VSEL-depleted BM-MNC or scaffold alone, and bone healing was evaluated at 8 weeks post-surgery. RESULTS:Bone healing was significantly increased in defects treated with VSEL and BM-MNC, compared to defects treated with VSEL-depleted BM-MNC. Donor cells were detected in new bone tissue, in all the defects treated with cells, and in fibrous tissue only in defects treated with VSEL-depleted BM-MNC. The number of CD68+ cells was the highest in the VSEL-depleted group, whereas the number of TRAP positive cells was the lowest in this group. CONCLUSIONS:Based on the results, we can conclude that VSEL play a role in BM-MNC induced bone formation. In our rat femur defect model, in defects treated with VSEL-depleted BM-MNC, osteoclastogenesis and bone formation were decreased, and foreign body reaction was increased.
journal_name
Stem Cell Rev Repjournal_title
Stem cell reviews and reportsauthors
Leppik L,Sielatycka K,Henrich D,Han Z,Wang H,Eischen-Loges MJ,Oliveira KMC,Bhavsar MB,Ratajczak MZ,Barker JHdoi
10.1007/s12015-019-09943-xsubject
Has Abstractpub_date
2020-02-01 00:00:00pages
198-211issue
1eissn
2629-3269issn
2629-3277pii
10.1007/s12015-019-09943-xjournal_volume
16pub_type
杂志文章abstract：:Trithorax group (TrxG) proteins play critical roles in transcriptional activation by promoting methylation of histone H3 Lysine 4 (H3K4), but the precise functions of the individual TrxG members during embryonic differentiation are not fully understood. Here we show that Mll2, a TrxG member, is required for proliferat...
journal_title：Stem cell reviews and reports
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abstract：:The combination of cell therapy with growth factors could be a useful approach to treat progressive muscular dystrophies. Here, we demonstrate, for the first time, that IGF-1 considerably enhances the myogenesis of human umbilical cord (UC) mesenchymal stromal cells (MSCs) in vitro and that IGF-1 enhances interaction ...
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abstract：:The reprogramming of adult somatic cells into an embryonic stem cell (ESC) state by various means has opened a new chapter in basic and applied life science. While this technology will create great opportunities for regenerative medicine, the more immediate impact is likely to be found in human disease modeling and dr...
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journal_title：Stem cell reviews and reports
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journal_title：Stem cell reviews and reports
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journal_title：Stem cell reviews and reports
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更新日期：2015-04-01 00:00:00
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journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
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journal_title：Stem cell reviews and reports
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更新日期：2019-06-01 00:00:00
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journal_title：Stem cell reviews and reports
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更新日期：2017-02-01 00:00:00
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journal_title：Stem cell reviews and reports
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journal_title：Stem cell reviews and reports
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journal_title：Stem cell reviews and reports
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