Abstract：
:Recent advances on human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have brought us closer to the realization of their clinical potential. Nonetheless, tissue engineering and regenerative medicine applications will require the generation of hPSC products well beyond the laboratory scale. This also mandates the production of hPSC therapeutics in fully-defined, xeno-free systems and in a reproducible manner. Toward this goal, we summarize current developments in defined media free of animal-derived components for hPSC culture. Bioinspired and synthetic extracellular matrices for the attachment, growth and differentiation of hPSCs are also reviewed. Given that most progress in xeno-free medium and substrate development has been demonstrated in two-dimensional rather than three dimensional culture systems, translation from the former to the latter poses unique difficulties. These challenges are discussed in the context of cultivation platforms of hPSCs as aggregates, on microcarriers or after encapsulation in biocompatible scaffolds.
journal_name
Stem Cell Rev Repjournal_title
Stem cell reviews and reportsauthors
Fan Y,Wu J,Ashok P,Hsiung M,Tzanakakis ESdoi
10.1007/s12015-014-9544-xsubject
Has Abstractpub_date
2015-02-01 00:00:00pages
96-109issue
1eissn
2629-3269issn
2629-3277journal_volume
11pub_type
杂志文章,评审abstract：:Cirrhosis is associated with dysregulated immune cell activation and immune dysfunction. These conditions modify gut flora, facilitate bacterial translocation, and increase susceptibility to bacterial peritonitis and consequent systemic infections by dramatically affecting long-term patient survival. Human amnion-deri...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-020-10104-8
更新日期：2021-01-03 00:00:00
abstract：:Since its early days, stem cell research, particularly human embryonic stem cell research, has been the focus of intense social debate, and the question of the moral status of the embryo has been a central issue in the controversy. Despite this friction, and while it has yet to obtain widespread success in clinical ap...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-009-9062-4
更新日期：2009-06-01 00:00:00
abstract：:Oligodendrocyte progenitor cells (OPCs) play a pivotal role in both health and disease within the central nervous system, with oligodendrocytes, arising from resident OPCs, being the main myelinating cell type. Disruption in OPC numbers can lead to various deleterious health defects. Numerous studies have described te...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-017-9742-4
更新日期：2017-08-01 00:00:00
abstract：:The molecular profile of human mesenchymal stem cells (MSCs) have emerged as a key factor in defining their identity. Nevertheless, the effect of fetal bovine serum (FBS) batches or origin on MSC molecular signature has been neglected. In this frame, chemical fingerprint of FBS batches from unrelated countries showed ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-018-9822-0
更新日期：2018-12-01 00:00:00
abstract：:There is a need for physiologically relevant assay platforms to provide functionally relevant models of diabetes, to accelerate the discovery of new treatment options and boost developments in drug discovery. In this review, we compare several 3D-strategies that have been used to increase the functional relevance of e...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-017-9783-8
更新日期：2018-04-01 00:00:00
abstract：:Reliable and efficient DNA and RNA transfection methods are required when studying the role of individual genes in mouse pluripotent stem cells. However, these cells usually grow in tight clusters and are therefore more difficult to transfect than many other cell lines. We have found that transfection is especially ch...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-016-9673-5
更新日期：2016-10-01 00:00:00
abstract：BACKGROUND:Bone marrow-derived mononuclear cells (BM-MNC) consist of a heterogeneous mix of mesenchymal stem cells (MSC), hematopoietic progenitor cells (HPC), endothelial progenitor cells (EPC), monocytes, lymphocytes and pluripotent stem cells. Whereas the importance of MSC and EPC has been well documented in bone he...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-019-09943-x
更新日期：2020-02-01 00:00:00
abstract：:During the process of development, neural crest cells migrate out from their niche between the newly formed ectoderm and the neural tube. Thereafter, they give rise not only to ectodermal cell types, but also to mesodermal cell types. Cell types with neural crest ancestry consequently comprise a number of specialized ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-011-9340-9
更新日期：2012-09-01 00:00:00
abstract：:Chronic articular cartilage defects are the most common disabling conditions of humans in the western world. The incidence for cartilage defects is increasing with age and the most prominent risk factors are overweight and sports associated overloading. Damage of articular cartilage frequently leads to osteoarthritis ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-012-9405-4
更新日期：2012-12-01 00:00:00
abstract：:Bioactive phospholipids, including sphingosine-1-phosphate (S1P), ceramide-1-phosphate (C1P), lysophosphatidylcholine (LPC), and its derivative lysophosphatidic acid (LPA), have emerged as important mediators regulating the trafficking of normal and cancer cells. While the role of S1P in regulating migration of hemato...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-018-9853-6
更新日期：2019-02-01 00:00:00
abstract：:Traumatic injuries are a leading cause of death and disability in both military and civilian populations. Given the complexity and diversity of traumatic injuries, novel and individualized treatment strategies are required to optimize outcomes. Cellular therapies have potential benefit for the treatment of acute or ch...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-020-10086-7
更新日期：2021-01-08 00:00:00
abstract：:Stem cells in the endometrium and myometrium possess an immense regenerative potential which is necessary to maintain the menstrual cycle and support pregnancy. These cells, as well as bone marrow stem cells, have also been implicated in the development of common benign gynecological disorders including leiomyomas, en...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-020-10075-w
更新日期：2020-11-05 00:00:00
abstract：:Converting adult cells from one cell type to another is a particularly interesting idea for regenerative medicine. Terminally differentiated cells can be induced to de-differentiate in vitro to become multipotent progenitors. In mammals these changes do not occur naturally, however exposing differentiated adult cells ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-014-9521-4
更新日期：2014-10-01 00:00:00
abstract：:The Networks of Centres of Excellence (NCE) is an innovative model of large-scale research networks that began in Canada in 1989, and has since been adopted by numerous countries around the globe. The Canadian Stem Cell Network (SCN), an NCE that has fostered stem cell research and innovation in Canada, has supported ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-015-9609-5
更新日期：2015-12-01 00:00:00
abstract：:Undifferentiated mouse embryonic stem cells (ESCs) possess low numbers of mitochondrial DNA (mtDNA), which encodes key subunits associated with the generation of ATP through oxidative phosphorylation (OXPHOS). As ESCs differentiate, mtDNA copy number is regulated by the nuclear-encoded mtDNA replication factors, which...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-011-9318-7
更新日期：2013-02-01 00:00:00
abstract：:Metastasis is the worst prognosis predictor in the clinical course of cancer development. Features of metastatic cancer cells include migratory ability, low degree of differentiation, self-renewal and proliferation potentials, as well as resistance to therapies. Metastatic cells do not present all of the necessary cha...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-019-09936-w
更新日期：2020-04-01 00:00:00
abstract：:Mesenchymal stem cells (MSCs) are promising tools for studying the mechanisms of development and for the regeneration of injured tissues. Correct selection of the MSCs source is crucial in order to obtain a more efficient treatment and, in this respect Periosteum-Derived Cells (PDPCs) may represent an interesting alte...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-012-9374-7
更新日期：2012-09-01 00:00:00
abstract：:Previous studies demonstrated that induced pluripotent stem (iPS) cells could produce viable mice through tetraploid complementation, which was thought to be the most stringent test for pluripotency. However, these highly pluripotent iPS cells were previously reported to be generated from fibroblasts of embryonic orig...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-010-9160-3
更新日期：2010-09-01 00:00:00
abstract：PURPOSE:Evaluation of potential tropic effects of vascular endothelial growth factor (VEGF) on the incorporation and differentiation of bone-marrow-derived stem cells (BMSCs) in a murine model of anterior ischemic optic neuropathy (AION). METHODS:In the first approach, small-sized subset of BMCs were isolated from GFP...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-012-9396-1
更新日期：2012-12-01 00:00:00
abstract：:Pre-clinical studies have supported the use of mesenchymal stem cells (MSC) to treat highly prevalent neurodegenerative diseases such as Parkinson's disease (PD) but preliminary trials have reported controversial results. In a rat model of PD induced by MPTP neurotoxin, we first observed a significant bilateral preser...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-011-9256-4
更新日期：2011-11-01 00:00:00
abstract：:The retinal pigment epithelium (RPE) is a highly specialized cell type located between the choroid and neural retina of the eye. RPE degeneration causes irreversible visual impairment, extending to blindness. Cell therapy has recently emerged as a potential therapeutic approach for retinal degeneration. MicroRNA-based...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-014-9568-2
更新日期：2015-06-01 00:00:00
abstract：:Mesenchymal stem cells (MSC) have been extensively studied over the past years for the treatment of different diseases. Most of the ongoing clinical trials currently involve the use of MSC derived from adult tissues. This source may have some limitations, particularly with therapies that may require extensive and repe...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-016-9695-z
更新日期：2017-02-01 00:00:00
abstract：:Nitric oxide (NO) is a gaseous free radical molecule involved in several biological processes related to inflammation, tissue damage, and infections. Based on reports that NO inhibits migration of granulocytes and monocytes, we became interested in the role of inducible NO synthetase (iNOS) in pharmacological mobiliza...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-016-9693-1
更新日期：2017-02-01 00:00:00
abstract：:Understanding the genetic and molecular mechanisms of ovarian cancer has been the focus of research efforts working toward the greater goal of improving cancer therapy for patients with residual disease after initial treatment with conventional surgery and neoadjuvant chemotherapy. The focus of this review will be cen...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-011-9344-5
更新日期：2012-09-01 00:00:00
abstract：:Orthotopic liver transplant (OLT) remains the standard of care for end stage liver disease. To circumvent allo-rejection, OLT subjects receive gluococorticoids (GC). We investigated the effects of GC on endogenous mesenchymal stem (stromal) cells (MSCs) in OLT. This question is relevant because MSCs have regenerative ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-017-9751-3
更新日期：2017-10-01 00:00:00
abstract：:Stem cell research has generated considerable attention for its potential to remediate many disorders of the central nervous system including neurodevelopmental disorders such as autism spectrum disorder (ASD) and cerebral palsy (CP) that place a high burden on individual children, families and society. Here we charac...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-015-9627-3
更新日期：2016-02-01 00:00:00
abstract：:The majority of patients survive an acute myocardial infarction (AMI). Their outcome is negatively influenced by post-AMI events, such as loss of viable cardiomyocytes due to a post-AMI inflammatory response, eventually resulting in heart failure and/or death. Recent pre-clinical animal studies indicate that mesenchym...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-014-9502-7
更新日期：2014-06-01 00:00:00
abstract：:Motor neuron loss or degeneration is the typical characteristic of amyotrophic lateral sclerosis (ALS), which often leads to weakness, paralysis, or even death. The underlying mechanisms of motor neuron degeneration and ALS progression remain elusive, and there is no effective treatment for ALS. The advances of stem c...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-019-09946-8
更新日期：2020-06-01 00:00:00
abstract：INTRODUCTION:Whether transplanted cardiac stem cells (CSCs) and mesenchymal stem cells (MSCs) improved ventricular fibrillation threshold (VFT) similarly is still unclear. We sought to compare the effects of the CSC and MSC transplantation on the electrophysiological characteristics and VFT in rats with myocardial infa...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-012-9367-6
更新日期：2013-06-01 00:00:00
abstract：:Metabolomic profiles of somatic cells, embryonic stem cells (ESCs), and induced pluripotent stem cells (iPSCs) reflect their metabolic phenotypes. The comparative study of metabolomes of these cells is important for understanding the differences in metabolism between somatic and pluripotent cells, and also the possibl...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-020-10052-3
更新日期：2020-12-01 00:00:00
