Abstract：
:Surface topography of Titanium (Ti) dental implants strongly influences osseointegration. In the present work, we have analyzed the influence of two Ti implant surfaces characterized by similar microtopography but different nanotopography and chemistry on the osteoblastic phenotype of Dental Pulp Stem Cells (DPSCs). The effect on osteogenic differentiation, extracellular matrix (ECM) and cell adhesion molecules production have been evaluated by means of molecular biology analyses. The morphology of the cells grown onto these surfaces has been analyzed with SEM and immunofluorescence (IF), and the safety of the surfaces has been tested by using karyotype analysis, Ames test and hemocompatibility assay. Results showed that starting from 15 days of DPSCs culture, a substantial expression of osteoblast specific markers and a strong increase of cell adhesion molecules can be detected. In particular, when DPSCs are seeded on the Ti implants expression of microRNA (miRNA)-196a, which is involved in osteoblastic commitment of stem cells, and of Vascular Cell Adhesion Molecule 1 (VCAM1), a factor involved in angiogenesis, is strongly enhanced.
journal_name
Stem Cell Rev Repjournal_title
Stem cell reviews and reportsauthors
Gardin C,Ferroni L,Piattelli A,SIvolella S,Zavan B,Mijiritsky Edoi
10.1007/s12015-016-9669-1subject
Has Abstractpub_date
2016-10-01 00:00:00pages
543-552issue
5eissn
2629-3269issn
2629-3277pii
10.1007/s12015-016-9669-1journal_volume
12pub_type
杂志文章abstract：:Motor neuron loss or degeneration is the typical characteristic of amyotrophic lateral sclerosis (ALS), which often leads to weakness, paralysis, or even death. The underlying mechanisms of motor neuron degeneration and ALS progression remain elusive, and there is no effective treatment for ALS. The advances of stem c...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-019-09946-8
更新日期：2020-06-01 00:00:00
abstract：:The retinal pigment epithelium (RPE) is a highly specialized cell type located between the choroid and neural retina of the eye. RPE degeneration causes irreversible visual impairment, extending to blindness. Cell therapy has recently emerged as a potential therapeutic approach for retinal degeneration. MicroRNA-based...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-014-9568-2
更新日期：2015-06-01 00:00:00
abstract：:Human embryonic stem cells (hESCs) can be propagated indefinitely in vitro in an undifferentiated pluripotent state, can differentiate into derivatives of all three germ layers and are of considerable interest for applications in regenerative medicine. Clinical application of hESCs, however, requires reliable protocol...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-011-9230-1
更新日期：2011-09-01 00:00:00
abstract：BACKGROUND:Bone marrow-derived mononuclear cells (BM-MNC) consist of a heterogeneous mix of mesenchymal stem cells (MSC), hematopoietic progenitor cells (HPC), endothelial progenitor cells (EPC), monocytes, lymphocytes and pluripotent stem cells. Whereas the importance of MSC and EPC has been well documented in bone he...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-019-09943-x
更新日期：2020-02-01 00:00:00
abstract：PURPOSE:Evaluation of potential tropic effects of vascular endothelial growth factor (VEGF) on the incorporation and differentiation of bone-marrow-derived stem cells (BMSCs) in a murine model of anterior ischemic optic neuropathy (AION). METHODS:In the first approach, small-sized subset of BMCs were isolated from GFP...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-012-9396-1
更新日期：2012-12-01 00:00:00
abstract：:Understanding the healthy and diseased state of skin is important in many areas of basic and applied research. Although the field of skin tissue engineering has advanced greatly over the last years, current in vitro skin models still do not mimic the complexity of the human skin. Skin-on-chip and induced pluripotent s...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-017-9737-1
更新日期：2017-06-01 00:00:00
abstract：:The practice of travelling abroad to receive unproven and unregulated stem cell treatments has become an increasingly problematic global phenomenon known as 'stem cell tourism'. In this paper, we examine representations of nine major clinics and providers of such treatments on the microblogging network Twitter. We col...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-014-9534-z
更新日期：2014-12-01 00:00:00
abstract：:The combination of cell therapy with growth factors could be a useful approach to treat progressive muscular dystrophies. Here, we demonstrate, for the first time, that IGF-1 considerably enhances the myogenesis of human umbilical cord (UC) mesenchymal stromal cells (MSCs) in vitro and that IGF-1 enhances interaction ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-012-9380-9
更新日期：2013-02-01 00:00:00
abstract：:Cell-based therapy is a promising approach for many diseases, including ischemic heart disease. Cardiac mesoangioblasts are committed vessel-associated progenitors that can restore to a significant, although partial, extent, heart structure and function in a murine model of myocardial infarction. Low-intensity pulsed ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-015-9608-6
更新日期：2015-12-01 00:00:00
abstract：:Reliable and efficient DNA and RNA transfection methods are required when studying the role of individual genes in mouse pluripotent stem cells. However, these cells usually grow in tight clusters and are therefore more difficult to transfect than many other cell lines. We have found that transfection is especially ch...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-016-9673-5
更新日期：2016-10-01 00:00:00
abstract：:Schwann cells (SC), the glial cells of peripheral nerves, are involved in many diseases including Charcot Marie Tooth and neurofibromatosis, and play a pivotal role in peripheral nerve regeneration. Although it is possible to obtain human SC from nerve biopsies, they are difficult to maintain and expand in culture. He...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-010-9198-2
更新日期：2011-06-01 00:00:00
abstract：:The COVID-19 pandemic has profoundly influenced public health and contributed to global economic divergences of unprecedented dimensions. Due to the high prevalence and mortality rates, it is then expected that the consequence and public health challenges will last for long periods. The rapid global spread of COVID-19...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-020-10108-4
更新日期：2021-01-05 00:00:00
abstract：:The molecular profile of human mesenchymal stem cells (MSCs) have emerged as a key factor in defining their identity. Nevertheless, the effect of fetal bovine serum (FBS) batches or origin on MSC molecular signature has been neglected. In this frame, chemical fingerprint of FBS batches from unrelated countries showed ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-018-9822-0
更新日期：2018-12-01 00:00:00
abstract：BACKGROUND:Mesenchymal stem cells derived from the chorionic villi of human term placenta (pMSCs) have drawn considerable interest because of their multipotent differentiation potential and their immunomodulatory capacity. These properties are the foundation for their clinical application in the fields of stem cell tra...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-014-9562-8
更新日期：2015-06-01 00:00:00
abstract：:Orthotopic liver transplant (OLT) remains the standard of care for end stage liver disease. To circumvent allo-rejection, OLT subjects receive gluococorticoids (GC). We investigated the effects of GC on endogenous mesenchymal stem (stromal) cells (MSCs) in OLT. This question is relevant because MSCs have regenerative ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-017-9751-3
更新日期：2017-10-01 00:00:00
abstract：:Adipogenesis is a complex process whereby the multipotent adipose-derived stem cell is converted to a preadipocyte before terminal differentiation into the mature adipocyte. Preadipocytes are present throughout adult life, exhibit adipose fat depot specificity, and differentiate and proliferate from distinct progenito...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-017-9774-9
更新日期：2018-02-01 00:00:00
abstract：:The development of pluripotent cells that enable stem cell research (SCR) without destroying human embryos is now a leading priority for science. Public and political controversies associated with human embryonic SCR experienced in the recent past should be alleviated if scientists no longer need to harvest cells from...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-013-9445-4
更新日期：2013-10-01 00:00:00
abstract：:Multipotent mesenchymal stem/stromal cells (MSCs) have regenerative and immunomodulatory properties to restore and repair injured tissues, making them attractive candidates for cell-based therapies. Experimental and clinical evidence has demonstrated the effectiveness of MSC transplantation in managing diabetes mellit...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-018-9869-y
更新日期：2019-04-01 00:00:00
abstract：:Mesenchymal stem cells (MSCs) are promising tools for studying the mechanisms of development and for the regeneration of injured tissues. Correct selection of the MSCs source is crucial in order to obtain a more efficient treatment and, in this respect Periosteum-Derived Cells (PDPCs) may represent an interesting alte...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-012-9374-7
更新日期：2012-09-01 00:00:00
abstract：:Recent advances on human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have brought us closer to the realization of their clinical potential. Nonetheless, tissue engineering and regenerative medicine applications will require the generation of ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-014-9544-x
更新日期：2015-02-01 00:00:00
abstract：:The reprogramming of adult somatic cells into an embryonic stem cell (ESC) state by various means has opened a new chapter in basic and applied life science. While this technology will create great opportunities for regenerative medicine, the more immediate impact is likely to be found in human disease modeling and dr...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-011-9342-7
更新日期：2013-08-01 00:00:00
abstract：:Human pluripotent stem cells (PSCs) are critical in vitro tools for understanding mechanisms that regulate lineage differentiation in the human embryo as well as a potentially unlimited supply of stem cells for regenerative medicine. Pluripotent human and mouse embryonic stem cells (ESCs) derived from the inner cell m...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-013-9490-z
更新日期：2014-04-01 00:00:00
abstract：:The Networks of Centres of Excellence (NCE) is an innovative model of large-scale research networks that began in Canada in 1989, and has since been adopted by numerous countries around the globe. The Canadian Stem Cell Network (SCN), an NCE that has fostered stem cell research and innovation in Canada, has supported ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-015-9609-5
更新日期：2015-12-01 00:00:00
abstract：:Various strategies have been published enabling cardiomyocyte differentiation of human induced pluripotent stem (iPS) cells. However the complex nature of signaling pathways involved as well as line-to-line variability compromises the application of a particular protocol to robustly obtain cardiomyocytes from multiple...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-014-9564-6
更新日期：2015-08-01 00:00:00
abstract：:Neural progenitor cells (NPCs) are multipotent cells that have the potential to produce neurons and glial cells in the neural system. NPCs undergo identity maintenance or differentiation regulated by different kinds of transcription factors. Here we present evidence that ETV5, which is an ETS transcription factor, pro...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-019-09904-4
更新日期：2019-10-01 00:00:00
abstract：:Zinc-finger nucleases (ZFNs) are sequence-specific genome engineering tools with great potential for the development of gene therapies. The achievement of permanent cures through gene therapy requires targeting of stem cells but the effects and/or side effects of ZFN treatment on adult stem cell potency are largely un...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-011-9313-z
更新日期：2012-06-01 00:00:00
abstract：:An unfortunate emergence of a new virus SARS-CoV-2, causing a disease known as COVID-19, has spread all around the globe and has caused a pandemic. It primarily affects the respiratory tract and lungs in some cases causing severe organ damage and pneumonia due to overwhelming immune responses. Clinical reports show th...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-020-10017-6
更新日期：2020-08-01 00:00:00
abstract：:The stem-cell-based tissue-engineering approaches are widely applied in establishing functional organs and tissues for regenerative medicine. Successful generation of induced pluripotent stem cells (iPS cells) and rapid progress of related technical platform provide great promise in the development of regenerative med...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-014-9531-2
更新日期：2014-10-01 00:00:00
abstract：:Hematopoietic stem cells (HSCs) from umbilical cord blood have been successfully used to treat blood disorders but one major hurdle is the relatively low cell dose available. Double cord blood unit transplantation results in elevated engraftment failure because one unit predominates over the other. Various approaches ...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章
doi：10.1007/s12015-013-9426-7
更新日期：2013-04-01 00:00:00
abstract：:Derivation of human embryonic stem cell (hES) lines in 1998 was not only a major technological breakthrough in the field of regenerative medicine; it also triggered a passionate debate about the ethical issues associated with the utilization of human embryos for derivation of hESC lines. Successful derivation of induc...
journal_title：Stem cell reviews and reports
pub_type： 杂志文章,评审
doi：10.1007/s12015-017-9766-9
更新日期：2017-12-01 00:00:00
