Abstract:
:The use of sugar-functionalized polyplexes as a nonviral gene delivery vector with lower cytotoxicity than the well-known polymeric carrier branched polyethyleneimine (BPEI) is investigated. The substitution of primary amine groups in the BPEI chains with lactose residues leads to larger polyplexes, presumably due to the higher amount of polymer required to complete DNA condensation. Nevertheless, the sugar functionalization substantially reduces the cytotoxicity of the assemblies. The nanocomplexes are taken up by the cells to a greater extent, whereas the levels of gene expression are maintained compared to those obtained using BPEI, which is known for its excellent transfection efficiency. Accordingly, the preparation of lower-cytotoxicity polyplexes while maintaining gene expression, which is highly relevant to the field, is demonstrated.
journal_name
Macromol Bioscijournal_title
Macromolecular bioscienceauthors
Albuquerque LJC,Alavarse AC,Carlan da Silva MC,Zilse MS,Barth MT,Bellettini IC,Giacomelli FCdoi
10.1002/mabi.201700299subject
Has Abstractpub_date
2018-02-01 00:00:00issue
2eissn
1616-5187issn
1616-5195journal_volume
18pub_type
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