Proliferation and differentiation of embryonic chick sympathetic neurons: effects of ciliary neurotrophic factor.


:At early developmental stages (embryonic day 7, E7), chick paravertebral sympathetic ganglia contain a cell population that divides in culture while expressing various neuronal properties. In an attempt to identify factors that control neuronal proliferation, we found that ciliary neurotrophic factor (CNTF) specifically inhibits the proliferation of those cells expressing neuronal markers. In addition, CNTF affects the differentiation of sympathetic ganglion cells by inducing the expression of vasoactive intestinal peptide immunoreactivity (VIP-IR). After 1 day in culture, tyrosine hydroxylase immunoreactivity (TH-IR) was expressed by about 86% of the cells whereas VIP-IR was virtually absent. In the presence of CNTF, 50%-60% of the cells expressed VIP-IR after 4 days in culture; however, none of the cells expressed VIP-IR in the absence of CNTF. These results, and the demonstration of cells that express both VIP and TH-IR, indicate that VIP is induced in cells that initially express tyrosine hydroxylase. The findings suggest a potential role for CNTF as a factor affecting the proliferation and differentiation of developing sympathetic neurons.






Ernsberger U,Sendtner M,Rohrer H




Has Abstract


1989-03-01 00:00:00
















  • A role for proteoglycans in the guidance of a subset of pioneer axons in cultured embryos of the cockroach.

    abstract::Several molecules involved in the development of the nervous system have specific binding sites for the glycosaminoglycan (GAG) side chains of proteoglycans. Exogenous GAGs should bind to these sites, competitively inhibit interactions with proteoglycans, and perturb development. GAGs added to the culture medium pertu...


    pub_type: 杂志文章


    authors: Wang L,Denburg JL

    更新日期:1992-04-01 00:00:00

  • Calcium Channels, Synaptic Plasticity, and Neuropsychiatric Disease.

    abstract::Voltage-gated calcium channels couple depolarization of the cell-surface membrane to entry of calcium, which triggers secretion, contraction, neurotransmission, gene expression, and other physiological responses. They are encoded by ten genes, which generate three voltage-gated calcium channel subfamilies: CaV1; CaV2;...


    pub_type: 杂志文章,评审


    authors: Nanou E,Catterall WA

    更新日期:2018-05-02 00:00:00

  • Multifocal attention filters targets from distracters within and beyond primate MT neurons' receptive field boundaries.

    abstract::Visual attention has been classically described as a spotlight that enhances the processing of a behaviorally relevant object. However, in many situations, humans and animals must simultaneously attend to several relevant objects separated by distracters. To account for this ability, various models of attention have b...


    pub_type: 杂志文章


    authors: Niebergall R,Khayat PS,Treue S,Martinez-Trujillo JC

    更新日期:2011-12-22 00:00:00

  • Fool me once, shame on you; fool me twice, shame on oxytocin.

    abstract::In this issue of Neuron, a study by Baumgartner et al. investigates the influence of oxytocin on trust behavior and its neural mechanisms. The authors report that, following breaches of trust, oxytocin facilitates prosocial behavior while modulating neural signals in the amygdala and caudate nucleus. The findings have...


    pub_type: 评论,杂志文章


    authors: Delgado MR

    更新日期:2008-05-22 00:00:00

  • Beyond the Stimulus: A Neurohumanities Approach to Language, Music, and Emotion.

    abstract::The brain basis of language, music, and emotion can be studied from the perspective of the psychological and cognitive sciences. Does this approach link to concerns of the humanities meaningfully? We outline prospects of developing a genuine neurohumanities research program. ...


    pub_type: 杂志文章


    authors: Hartley CA,Poeppel D

    更新日期:2020-11-25 00:00:00

  • PIP kinase Igamma is the major PI(4,5)P(2) synthesizing enzyme at the synapse.

    abstract::Disruption of the presynaptically enriched polyphosphoinositide phosphatase synaptojanin 1 leads to an increase of clathrin-coated intermediates and of polymerized actin at endocytic zones of nerve terminals. These changes correlate with elevated levels of PI(4,5)P(2) in neurons. We report that phosphatidylinositol ph...


    pub_type: 杂志文章


    authors: Wenk MR,Pellegrini L,Klenchin VA,Di Paolo G,Chang S,Daniell L,Arioka M,Martin TF,De Camilli P

    更新日期:2001-10-11 00:00:00

  • A novel family of candidate pheromone receptors in mammals.

    abstract::Pheromonal activation of the vomeronasal organ (VNO) elicits genetically preprogrammed behaviors and physiological changes in mammals. We have identified a novel gene family encoding over one hundred VNO specific receptors, the V3Rs. V3R sequences are highly similar to each other and appear distantly related to the pu...


    pub_type: 杂志文章


    authors: Pantages E,Dulac C

    更新日期:2000-12-01 00:00:00

  • Mitochondrial dysfunction in NnaD mutant flies and Purkinje cell degeneration mice reveals a role for Nna proteins in neuronal bioenergetics.

    abstract::The Purkinje cell degeneration (pcd) mouse is a recessive model of neurodegeneration, involving cerebellum and retina. Purkinje cell death in pcd is dramatic, as >99% of Purkinje neurons are lost in 3 weeks. Loss of function of Nna1 causes pcd, and Nna1 is a highly conserved zinc carboxypeptidase. To determine the bas...


    pub_type: 杂志文章


    authors: Chakrabarti L,Zahra R,Jackson SM,Kazemi-Esfarjani P,Sopher BL,Mason AG,Toneff T,Ryu S,Shaffer S,Kansy JW,Eng J,Merrihew G,MacCoss MJ,Murphy A,Goodlett DR,Hook V,Bennett CL,Pallanck LJ,La Spada AR

    更新日期:2010-06-24 00:00:00

  • Dendritic GABA release depresses excitatory transmission between layer 2/3 pyramidal and bitufted neurons in rat neocortex.

    abstract::GABAergic, somatostatin-containing bitufted interneurons in layer 2/3 of rat neocortex are excited via glutamatergic excitatory postsynaptic potentials (EPSPs) by pyramidal neurons located in the same cortical layer. Pair recordings showed that short bursts of backpropagating dendritic action potentials (APs) reduced ...


    pub_type: 杂志文章


    authors: Zilberter Y,Kaiser KM,Sakmann B

    更新日期:1999-12-01 00:00:00

  • The ubiquitin ligase Phr1 regulates axon outgrowth through modulation of microtubule dynamics.

    abstract::To discover new genes involved in axon navigation, we conducted a forward genetic screen for recessive alleles affecting motor neuron pathfinding in GFP reporter mice mutagenized with ENU. In Magellan mutant embryos, motor axons were error prone and wandered inefficiently at choice points within embryos, but paradoxic...


    pub_type: 杂志文章


    authors: Lewcock JW,Genoud N,Lettieri K,Pfaff SL

    更新日期:2007-11-21 00:00:00

  • Calcium modulation and high calcium permeability of neuronal nicotinic acetylcholine receptors.

    abstract::Two properties were found to distinguish neuronal from muscle nicotinic acetylcholine receptors (nAChRs). First, neuronal nAChRs have a greater Ca2+ permeability. The high Ca2+ flux through neuronal nAChRs activates a Ca(2+)-dependent Cl- conductance, and the Ca2+ to Cs+ permeability ratio (PCa/PCs) is 7 times greater...


    pub_type: 杂志文章


    authors: Vernino S,Amador M,Luetje CW,Patrick J,Dani JA

    更新日期:1992-01-01 00:00:00

  • PDZ-containing proteins provide a functional postsynaptic scaffold for nicotinic receptors in neurons.

    abstract::Protein scaffolds are essential for specific and efficient downstream signaling at synapses. Though nicotinic receptors are widely expressed in the nervous system and influence numerous cellular events due in part to their calcium permeability, no scaffolds have yet been identified for the receptors in neurons. Here w...


    pub_type: 杂志文章


    authors: Conroy WG,Liu Z,Nai Q,Coggan JS,Berg DK

    更新日期:2003-06-05 00:00:00

  • Area specificity and topography of thalamocortical projections are controlled by ephrin/Eph genes.

    abstract::The mechanisms generating precise connections between specific thalamic nuclei and cortical areas remain poorly understood. Using axon tracing analysis of ephrin/Eph mutant mice, we provide in vivo evidence that Eph receptors in the thalamus and ephrins in the cortex control intra-areal topographic mapping of thalamoc...


    pub_type: 评论,杂志文章


    authors: Dufour A,Seibt J,Passante L,Depaepe V,Ciossek T,Frisén J,Kullander K,Flanagan JG,Polleux F,Vanderhaeghen P

    更新日期:2003-07-31 00:00:00

  • Closed-loop and activity-guided optogenetic control.

    abstract::Advances in optical manipulation and observation of neural activity have set the stage for widespread implementation of closed-loop and activity-guided optical control of neural circuit dynamics. Closing the loop optogenetically (i.e., basing optogenetic stimulation on simultaneously observed dynamics in a principled ...


    pub_type: 杂志文章,评审


    authors: Grosenick L,Marshel JH,Deisseroth K

    更新日期:2015-04-08 00:00:00

  • A Visual Circuit Related to Habenula Underlies the Antidepressive Effects of Light Therapy.

    abstract::Light plays a pivotal role in the regulation of affective behaviors. However, the precise circuits that mediate the impact of light on depressive-like behaviors are not well understood. Here, we show that light influences depressive-like behaviors through a disynaptic circuit linking the retina and the lateral habenul...


    pub_type: 杂志文章


    authors: Huang L,Xi Y,Peng Y,Yang Y,Huang X,Fu Y,Tao Q,Xiao J,Yuan T,An K,Zhao H,Pu M,Xu F,Xue T,Luo M,So KF,Ren C

    更新日期:2019-04-03 00:00:00

  • Role for Wnt Signaling in Retinal Neuropil Development: Analysis via RNA-Seq and In Vivo Somatic CRISPR Mutagenesis.

    abstract::Screens for genes that orchestrate neural circuit formation in mammals have been hindered by practical constraints of germline mutagenesis. To overcome these limitations, we combined RNA-seq with somatic CRISPR mutagenesis to study synapse development in the mouse retina. Here synapses occur between cellular layers, f...


    pub_type: 杂志文章


    authors: Sarin S,Zuniga-Sanchez E,Kurmangaliyev YZ,Cousins H,Patel M,Hernandez J,Zhang KX,Samuel MA,Morey M,Sanes JR,Zipursky SL

    更新日期:2018-04-04 00:00:00

  • Hypocretin/Orexin excites hypocretin neurons via a local glutamate neuron-A potential mechanism for orchestrating the hypothalamic arousal system.

    abstract::Neurons that release hypocretin/orexin modulate sleep, arousal, and energy homeostasis; the absence of hypocretin results in narcolepsy. Here we present data on the physiological characteristics of these cells, identified with GFP in transgenic mouse brain slices. Hypocretin-1 and -2 depolarized hypocretin neurons by ...


    pub_type: 杂志文章


    authors: Li Y,Gao XB,Sakurai T,van den Pol AN

    更新日期:2002-12-19 00:00:00

  • Innovations in the imaging of brain functions using fluorescent proteins.

    abstract::Fluorescence imaging has enabled us to decipher spatiotemporal information coded in complex tissues. Genetically encoded probes that enable fluorescence imaging of excitable cell activity have been constructed by fusing fluorescent proteins to functional proteins that are involved in physiological signaling. The probe...


    pub_type: 杂志文章,评审


    authors: Miyawaki A

    更新日期:2005-10-20 00:00:00

  • A neural representation of prior information during perceptual inference.

    abstract::Perceptual inference is biased by foreknowledge about what is probable or possible. How prior expectations are neurally represented during visual perception, however, remains unknown. We used functional magnetic resonance imaging to measure brain activity in humans judging simple visual stimuli. Perceptual decisions w...


    pub_type: 杂志文章


    authors: Summerfield C,Koechlin E

    更新日期:2008-07-31 00:00:00

  • Synaptotagmins: That's Why So Many.

    abstract::Synaptotagmin 7 (Syt7) was originally identified as a slow Ca2+ sensor for lysosome fusion, but its function at fast synapses is controversial. The paper by Luo and Südhof (2017) in this issue of Neuron shows that at the calyx of Held in the auditory brainstem Syt7 triggers asynchronous release during stimulus trains,...


    pub_type: 杂志文章


    authors: Chen C,Jonas P

    更新日期:2017-05-17 00:00:00

  • Morphogenetic and cellular movements that shape the mouse cerebellum; insights from genetic fate mapping.

    abstract::We used the cerebellum as a model to study the morphogenetic and cellular processes underlying the formation of elaborate brain structures from a simple neural tube, using an inducible genetic fate mapping approach in mouse. We demonstrate how a 90 degrees rotation between embryonic days 9 and 12 converts the rostral-...


    pub_type: 杂志文章


    authors: Sgaier SK,Millet S,Villanueva MP,Berenshteyn F,Song C,Joyner AL

    更新日期:2005-01-06 00:00:00

  • Partially Mixed Selectivity in Human Posterior Parietal Association Cortex.

    abstract::To clarify the organization of motor representations in posterior parietal cortex, we test how three motor variables (body side, body part, cognitive strategy) are coded in the human anterior intraparietal cortex. All tested movements were encoded, arguing against strict anatomical segregation of effectors. Single uni...


    pub_type: 杂志文章


    authors: Zhang CY,Aflalo T,Revechkis B,Rosario ER,Ouellette D,Pouratian N,Andersen RA

    更新日期:2017-08-02 00:00:00

  • Argiotoxin detects molecular differences in AMPA receptor channels.

    abstract::Argiotoxin, a component of the spider venom from Argiope lobata, blocks AMPA receptor channels expressed in homomeric and heteromeric configuration in Xenopus oocytes. Argiotoxin acts as an open channel blocker in a voltage-dependent manner and discriminates between the functionally diverse AMPA receptors. Importantly...


    pub_type: 杂志文章


    authors: Herlitze S,Raditsch M,Ruppersberg JP,Jahn W,Monyer H,Schoepfer R,Witzemann V

    更新日期:1993-06-01 00:00:00

  • Real-Time Imaging Reveals Properties of Glutamate-Induced Arc/Arg 3.1 Translation in Neuronal Dendrites.

    abstract::The immediate early gene Arc (also Arg3.1) produces rapid changes in synaptic properties that are linked to de novo translation. Here we develop a novel translation reporter that exploits the rapid maturation and "flash" kinetics of Gaussia luciferase (Gluc) to visualize Arc translation. Following glutamate stimulatio...


    pub_type: 杂志文章


    authors: Na Y,Park S,Lee C,Kim DK,Park JM,Sockanathan S,Huganir RL,Worley PF

    更新日期:2016-08-03 00:00:00

  • A plaque-specific antibody clears existing β-amyloid plaques in Alzheimer's disease mice.

    abstract::Aβ Immunotherapy is a promising therapeutic approach for Alzheimer's disease. Preclinical studies demonstrate that plaque prevention is possible; however, the more relevant therapeutic removal of existing plaque has proven elusive. Monoclonal antibodies in development target both soluble and insoluble Aβ peptide. We h...


    pub_type: 杂志文章


    authors: Demattos RB,Lu J,Tang Y,Racke MM,Delong CA,Tzaferis JA,Hole JT,Forster BM,McDonnell PC,Liu F,Kinley RD,Jordan WH,Hutton ML

    更新日期:2012-12-06 00:00:00

  • A Large-Scale Circuit Mechanism for Hierarchical Dynamical Processing in the Primate Cortex.

    abstract::We developed a large-scale dynamical model of the macaque neocortex, which is based on recently acquired directed- and weighted-connectivity data from tract-tracing experiments, and which incorporates heterogeneity across areas. A hierarchy of timescales naturally emerges from this system: sensory areas show brief, tr...


    pub_type: 杂志文章


    authors: Chaudhuri R,Knoblauch K,Gariel MA,Kennedy H,Wang XJ

    更新日期:2015-10-21 00:00:00

  • The internal quaternary ammonium receptor site of Shaker potassium channels.

    abstract::Quaternary ammonium (QA) compounds inhibit K+ conductance by entering and occluding the open pore of voltage-activated K+ channels. We characterized the effects of a series of alkyl-triethylammonium blockers on the Shaker K+ channel and tested them on a series of site-directed mutants of the channel protein in order t...


    pub_type: 杂志文章


    authors: Choi KL,Mossman C,Aubé J,Yellen G

    更新日期:1993-03-01 00:00:00

  • Charybdotoxin binding in the I(Ks) pore demonstrates two MinK subunits in each channel complex.

    abstract::I(Ks) voltage-gated K(+) channels contain four pore-forming KCNQ1 subunits and MinK accessory subunits in a number that has been controversial. Here, I(Ks) channels assembled naturally by monomer subunits are compared to those with linked subunits that force defined stoichiometries. Two strategies that exploit charybd...


    pub_type: 杂志文章


    authors: Chen H,Kim LA,Rajan S,Xu S,Goldstein SA

    更新日期:2003-09-25 00:00:00

  • In Situ Transcription Profiling of Single Cells Reveals Spatial Organization of Cells in the Mouse Hippocampus.

    abstract::Identifying the spatial organization of tissues at cellular resolution from single-cell gene expression profiles is essential to understanding biological systems. Using an in situ 3D multiplexed imaging method, seqFISH, we identify unique transcriptional states by quantifying and clustering up to 249 genes in 16,958 c...


    pub_type: 杂志文章


    authors: Shah S,Lubeck E,Zhou W,Cai L

    更新日期:2016-10-19 00:00:00

  • Rare complete knockouts in humans: population distribution and significant role in autism spectrum disorders.

    abstract::To characterize the role of rare complete human knockouts in autism spectrum disorders (ASDs), we identify genes with homozygous or compound heterozygous loss-of-function (LoF) variants (defined as nonsense and essential splice sites) from exome sequencing of 933 cases and 869 controls. We identify a 2-fold increase i...


    pub_type: 杂志文章


    authors: Lim ET,Raychaudhuri S,Sanders SJ,Stevens C,Sabo A,MacArthur DG,Neale BM,Kirby A,Ruderfer DM,Fromer M,Lek M,Liu L,Flannick J,Ripke S,Nagaswamy U,Muzny D,Reid JG,Hawes A,Newsham I,Wu Y,Lewis L,Dinh H,Gross S,W

    更新日期:2013-01-23 00:00:00