Multidirectional effects of calmodulin kinase II on transmitter release in mature and newly formed mouse motor synapses.

Abstract:

:Calmodulin inhibitor W-7 did not cause changes in the quantal content of postsynaptic end-plate potentials (EPP) in newly formed synapses, but prevented facilitation of acetylcholine secretion induced by L-type Ca(2+)channels blocker nitrendipine. CaMKII inhibitor KN-62 produced similar effect and suppressed the increase in EPP quantal content caused by blockade of L-type Ca(2+)channels. Phosphatase PP2A inhibitor okadaic acid significantly facilitated secretion in newly formed synapses; the effect was completely blocked by KN-62. In mature synapses, okadaic acid had no effect on transmitter secretion. KN-62 increased EPP quantal content. We hypothesize that CaMKII produced different effects on acetylcholine secretion in mature and immature synapses depending on specificity of calcium signaling and PP2A phosphatase activity.

journal_name

Bull Exp Biol Med

authors

Bogatcheva PO,Balezina OP

doi

10.1007/s10517-013-1940-8

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

316-9

issue

3

eissn

0007-4888

issn

1573-8221

journal_volume

154

pub_type

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