Multidirectional effects of calmodulin kinase II on transmitter release in mature and newly formed mouse motor synapses.

Abstract:

:Calmodulin inhibitor W-7 did not cause changes in the quantal content of postsynaptic end-plate potentials (EPP) in newly formed synapses, but prevented facilitation of acetylcholine secretion induced by L-type Ca(2+)channels blocker nitrendipine. CaMKII inhibitor KN-62 produced similar effect and suppressed the increase in EPP quantal content caused by blockade of L-type Ca(2+)channels. Phosphatase PP2A inhibitor okadaic acid significantly facilitated secretion in newly formed synapses; the effect was completely blocked by KN-62. In mature synapses, okadaic acid had no effect on transmitter secretion. KN-62 increased EPP quantal content. We hypothesize that CaMKII produced different effects on acetylcholine secretion in mature and immature synapses depending on specificity of calcium signaling and PP2A phosphatase activity.

journal_name

Bull Exp Biol Med

authors

Bogatcheva PO,Balezina OP

doi

10.1007/s10517-013-1940-8

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

316-9

issue

3

eissn

0007-4888

issn

1573-8221

journal_volume

154

pub_type

杂志文章
  • Hormonal reaction of the testes to chorionic gonadotropin in CBA/Lac and PT mice: effects of the dose and time after treatment.

    abstract::Testosterone concentrations in the blood of CBA/Lac and PT mice and its content in the testes under normal conditions and during stimulation with chorionic gonadotropin were measured by enzyme immunoassay. Genetic differences in reactivity of the testes to chorionic gonadotropin were revealed: PT mice were characteriz...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-010-1112-z

    authors: Dubovenko EA,Osadchuk LV

    更新日期:2010-12-01 00:00:00

  • Effect of factors produced by the placenta on cytokine secretion by THP-1 cells cultured on a 3D scaffold.

    abstract::We compared the effects of soluble products from the placenta obtained from women with normal pregnancy and pregnancy complicated by preeclampsia on cytokine secretion by THP-1 cells cultured on a 3D Matrigel scaffold. In the presence of soluble products from all placentas, the cells actively secreted IL-8, MCP-1, and...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-014-2397-0

    authors: Onokhina YS,L'vova TY,Tsitskarava DZ,Koren'kov DA,Sel'kov SA,Sokolov DI

    更新日期:2014-02-01 00:00:00

  • On the mechanism of anti-ischemic effects of afobazole.

    abstract::The anti-ischemic effect of synthetic and pharmacologically tested anxiolytic afobazole (10 mg/kg intravenously) was studied on anesthetized rats with acute endocardial ischemia caused by isoproterenol infusion (20 mg/kg/min). A calcium antagonist verapamil (1 mg/kg intravenously) belonging to the group of phenyl alky...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-013-2246-6

    authors: Seredenin SB,Tsorin IB,Vititnova MB,Stolyaruk VN,Chichkanov GG,Kryzhanovskii SA

    更新日期:2013-10-01 00:00:00

  • Chronotropic and dromotropic components of cardiac reflexes in the cat.

    abstract::The relationship between dromotropic and chronotropic components of various cardiac reflexes was studied in cats. Intravenous infusion of blood was mainly accompanied by unidirectional negative chronotropic and dromotropic effects, but the dynamics of these effects was different. Clumping of the carotid arteries in mo...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-008-0041-6

    authors: Alipov NN,Sergeeva OV,Bobrova NA,Kuznetsova TE,Smirnov VM

    更新日期:2008-02-01 00:00:00

  • Protective effect of complex antioxidant preparation containing vitamins and amino acids in rats with burn trauma complicated by endotoxemia.

    abstract::Burn trauma increased blood chemiluminescence, while lipopolysaccharide in a dose of 1 mg/kg potentiated this effect, activated LPO, and decreased plasma antioxidant activity. In erythrocytes, superoxide dismutase activity increased, while activity of peroxide-utilizing enzymes decreased. Myeloperoxidase content incre...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-006-0253-6

    authors: Mikhal'chik EV,Titkova SM,Anurov MV,Ettinger AP,Korkina LG

    更新日期:2006-06-01 00:00:00

  • Contribution of Hydrogen Sulfide to Dilation of Rat Cerebral Arteries after Ischemia/Reperfusion Injury.

    abstract::The study examined the effect of H2S on the tone of cerebral arteries in rats after global cerebral ischemia/reperfusion injury and cooperation between NO and H2S in the control over cerebral circulation during the postischemic period. In control sham-operated and in experimental rats with ischemia/reperfusion injury,...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-020-04759-z

    authors: Lobov GI,Sokolova IB,Gorshkova OP,Shvetsova ME,Dvoretskii DP

    更新日期:2020-03-01 00:00:00

  • Peculiarities of the effect of terahertz radiation at nitric oxide frequencies on gaseous and electrolyte composition of the blood in different types of stress.

    abstract::We studied the effect of terahertz radiation at NO frequencies 150.176-150.664 GHz on gaseous and electrolyte composition of the blood in albino rats during stress. Partial or complete recovery of the studied parameters of blood gases and electrolytes in stressed animals was observed after 15- and 30-min terahertz irr...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-010-1102-1

    authors: Kirichuk VF,Tsymbal AA

    更新日期:2010-12-01 00:00:00

  • Anticoagulant Effects of Arginine-Containing Peptides of the Glyproline Family (His-Phe-Arg-Trp-Pro-Gly-Pro and Thr-Lys-Pro-Arg-Pro-Gly-Pro) Revealed by Thromboelastography.

    abstract::Thromboelastography revealed anticoagulant effects of 3 glyproline oligopeptides (in various concentrations): His-Phe-Arg-Trp-Pro-Gly-Pro, Thr-Lys-Pro-Arg-Pro-Gly-Pro (Selank), and Pro-Gly-Pro. The parameters R, K, MA, S, TMA, and J changed to hypocoagulation direction in comparison to the control. At this, Selank dem...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-017-3950-4

    authors: Rogozinskaya EY,Lyapina MG

    更新日期:2017-12-01 00:00:00

  • Morphofunctional characteristics of fetoplacental barrier of placental villi during pregnancy complicated by herpes-virus infection.

    abstract::Analysis of placenta homogenate from pregnant women having an episode of herpes-virus infection during the third trimester revealed suppression of Bcl-2 protein activity and increase in caspase 3 concentration. Activity of Bcl-2 protein decreases with increasing the titer of antibodies to herpes virus. Increased caspa...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-010-0987-z

    authors: Lutsenko MT,Andrievskaya IA

    更新日期:2010-10-01 00:00:00

  • Evaluation of fibrogenic potential of industrial multi-walled carbon nanotubes in acute aspiration experiment.

    abstract::Local inflammatory response in the lungs and fi brogenic potential of multi-walled carbon nanotubes were studied in an acute aspiration experiment in mice. The doses were chosen based on the concentration of nanotubes in the air at a workplace of the company-producer. ELISA, fl ow cytometry, enhanced darkfield microsc...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-015-2835-7

    authors: Khaliullin TO,Shvedova AA,Kisin ER,Zalyalov RR,Fatkhutdinova LM

    更新日期:2015-03-01 00:00:00

  • Effect of basal hormone profile on the formation of the vaginal bacterial cenosis in women.

    abstract::Adhesion activity of L. acidophilus NK1, L. fermentum 90 TS4, and C. albicans 506 B on female vaginal epithelium was studied. Adhesion of various lactobacillus species was hormone-dependent. Adhesion of C. albicans 506 B was not associated with estrogen level. The effects of synthetic drugs and phytopreparations used ...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-012-1714-8

    authors: Rybas YA,Bustamante RA,Kravtsov EG,Tchernitchin AN,Dalin MV

    更新日期:2012-07-01 00:00:00

  • The Role of 5-HT1A Receptors in Long-Term Adaptation of Newborn Rats to Hypoxia.

    abstract::We studied the effects of neonatal hypoxia on adaptive behavior of rats during prepubertal and pubertal periods in the control and after repeated injections of 5-HT1A receptor agonist buspirone. Hypoxia enhanced the inflammatory nociceptive response and exacerbated the depressive-like behavior. Repeated injections of ...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-016-3445-8

    authors: Mikhailenko VA,Butkevich IP

    更新日期:2016-08-01 00:00:00

  • C-terminal Pro-Gly-Pro tripeptide in contrast to full-length neuropeptide semax exhibits no neuroprotective effect in experimental cerebral ischemia.

    abstract::The C-terminal fragment Pro-Gly-Pro of semax does not modulate the development of symptoms of neurological deficiency and mortality in rats with incomplete global cerebral ischemia. Hence, previously revealed neuroprotective effects of semax are mainly determined by corticotropin ACTH4-7 fragment. ...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-005-0311-5

    authors: Fadyukova OE,Kadi A,Bai O,Andzhusheva GM,Koshelev VB

    更新日期:2005-04-01 00:00:00

  • Glutathione system and activity of NADPH-generating enzymes in the liver of intact rats and animals with toxic hepatitis receiving melatonin.

    abstract::Melatonin administered to intact animals increased glutathione concentration and activities of glutathione peroxidase, glutathione reductase, NADP-isocitrate dehydrogenase, and glucose-6-phosphate dehydrogenase in the liver. In animals with toxic hepatitis melatonin treatment decreased glutathione concentration and en...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-005-0346-7

    authors: Pashkov AN,Popov SS,Semenikhina AV,Rakhmanova TI

    更新日期:2005-05-01 00:00:00

  • Location and intensity of IL-8 and TGF-beta2 mRNA production in the fimbrial compartment of fallopian tubes and IL-10 in the endometrium in patients with pyoinflammatory adnexal diseases.

    abstract::The location and level of IL-8 and TGF-beta2 expression in the fimbrial compartment of fallopian tubes and IL-10 expression in the endometrium of women with pyoinflammatory adnexal diseases were studied by in situ hybridization. These diseases are associated with considerable changes in the levels of local production ...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1023/a:1024193216377

    authors: Zhdanov AV,Kurbanova DF,Sosulina LY,Davydova MP,Ezhova LS,Sukhikh GT

    更新日期:2003-03-01 00:00:00

  • Mechanism of the protective effect of succinic semialdehyde and its derivatives in hypoxia.

    abstract::Preliminary injection of succinic semialdehyde into animals in experiments with hypoxia led to a lower concentration of free ammonia in their brain tissues than in control experiments in which the compound was not given. The enzyme system of the liver tissue catalyzes the oxidation of NAD-H-2 by succinic semialdeyde a...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/BF00799318

    authors: Zubovskaya AM,Ostrovskaya RU,Tsybina NM

    更新日期:1975-01-01 00:00:00

  • Oxyquinoline-Dependent Changes in Claudin-Encoding Genes Contribute to Impairment of the Barrier Function of the Trophoblast Monolayer.

    abstract::Natural response to hypoxia critically depends on rapid stabilization of hypoxia-inducible factor (HIF). Under normoxic conditions, HIF-prolyl hydroxylases mark α-subunits of HIF for degradation, while hypoxia results in stabilization of HIF-α. Oxyquinoline derivatives suppress activity of HIF-prolyl hydroxylases lead...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-019-04352-z

    authors: Knyazev EN,Petrov VA,Gazizov IN,Gerasimenko TN,Tsypina IM,Tonevitsky AG,Sukhikh GT

    更新日期:2019-01-01 00:00:00

  • Crystallization of oral fluid components in patients with type 2 diabetes mellitus.

    abstract::Crystal aggregations of oral fluid from normal subjects and patients with type 2 diabetes mellitus were examined. Morphological signs characterizing crystal aggregations of salivary pools from patients with type 2 diabetes mellitus are described and classified. ...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-005-0027-6

    authors: Selifanova EI,Barer GM,Mkrtumyan AM,Denisov AB

    更新日期:2004-09-01 00:00:00

  • Xenotransplantation of embryonic precursors of human myogenesis for the correction of dystrophinopathy in mice with hereditary muscular dystrophy.

    abstract::Human embryonic myogenic precursors were transplanted into muscles of mdx mice with hereditary dystrophin-deficient muscular dystrophy. Transplantation induced the synthesis of human dystrophin. The number of dystrophin-positive fibers progressively decreased, however, some of them were preserved even 5 months after t...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1023/a:1026009517850

    authors: Yarygin VN,Sukhikh GT,Sitnikov VF,Stenina MA,Kuznetsov AB,Poltavtseva RA,Krivov LI,Voevodin DA,Savchuk VI,Revishchin AV,Korochkin LI,Aleksandrova MA

    更新日期:2003-07-01 00:00:00

  • Detection of protein with anticonsolidation properties in the rat brain.

    abstract::Isolation and identification of rat brain protein regulated by serotonin-modulated protein SMP-69 is described. Intracerebral administration of anti-SMP-69 antibodies activates cerebral cortex cell genome and increases the content of electropheretic fraction 28. Intracerebral administration of isolated and purified pr...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/BF02766081

    authors: Mekhtiev AA

    更新日期:2000-08-01 00:00:00

  • Effect of pentobarbital on spectral characteristics and phase ratios of wave oscillations of cardiac contraction period and time of atrioventricular conduction in cats.

    abstract::Effects of pentobarbital on spectral characteristics and phase ratios of wave oscillations of the cardiac contraction period (RR interval) and time of atrioventricular conduction (AV interval) were studied in experiments of cats. Pentobarbital moderately reduced the mean values of both intervals and significantly redu...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-006-0151-y

    authors: Alipov NN,Sergeeva OV,Smirnov VM,Kuznetsova TE,Bobrova NA

    更新日期:2006-03-01 00:00:00

  • Sodium Dodecyl Sulfate Cytotoxicity towards HaCaT Keratinocytes: Comparative Analysis of Methods for Evaluation of Cell Viability.

    abstract::Viability of keratinocytes of HaCaT immortalized line incubated with sodium dodecyl sulfate for 3 min, 1 and 48 h, was studied by light microscopy, MTT test, and neutral red absorption test. The IC50 values were determined for each of the studied lengths of exposure. HaCaT cells exhibited a dose-dependent decrease of ...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-017-3785-z

    authors: Rusanov AL,Luzgina NG,Lisitsa AV

    更新日期:2017-06-01 00:00:00

  • Changes in thyroid status of rats after prolonged exposure to low dose dichlorodiphenyltrichloroethane.

    abstract::The effect of low dose dichlorodiphenyltrichloroethane (DDT), omnipresent ecotoxicant and endocrine disruptor, on the functioning of the endocrine system is an urgent problem. We studied the effect of low dose DDT on thyroid status in rats. Rats receiving DDT in a dose of 1.890±0.086 μg/kg for 6 weeks showed increased...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-014-2443-y

    authors: Yaglova NV,Yaglov VV

    更新日期:2014-04-01 00:00:00

  • Polymorphism of Matrix Metalloproteinases Genes MMP1, MMP2, MMP3, and MMP7 and the Risk of Varicose Veins of Lower Extremities.

    abstract::We studied the effects of single nucleotide polymorphisms in the promoter regions of matrix metalloproteinase genes rs1799750 (-1607dupG) MMP1, rs243865 (C-1306T) MMP2, rs3025058 (-1171dupA) MMP3, and rs11568818 (A-181G) MMP7 on the risk of varicose vein of the lower extremities in ethnical Russians, residents of the ...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-017-3871-2

    authors: Shadrina AS,Smetanina MA,Sevost'yanova KS,Shevela AI,Seliverstov EI,Zakharova EA,Voronina EN,Ilyukhin EA,Zolotukhin IA,Kirienko AI,Filipenko ML

    更新日期:2017-09-01 00:00:00

  • Nonviral transfection of adipose tissue stromal cells: an experimental study.

    abstract::Delivery of plasmid DNA and interfering RNA into adipose tissue stromal cells was carried out by the methods of lipofection, calcium phosphate method, and by electroporation. The percent of transfected cells after delivery of plasmid DNA by the calcium phosphate method and lipofection was 0 and 15%, respectively, vs. ...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-009-0546-7

    authors: Lopatina TV,Kalinina NI,Parfyonova EV

    更新日期:2009-04-01 00:00:00

  • A new method for screening diagnosis of insulin resistance.

    abstract::A simple method for the diagnosis of insulin resistance, easily realized in clinical practice, is developed in order to detect patients at a high risk of diseases associated with this condition. The metabolic index is estimated as the proportion of triglycerides and glucose values to quadratic HDL cholesterol value (i...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-015-2771-6

    authors: Roitberg GE,Dorosh ZhV,Sharkhun OO

    更新日期:2015-01-01 00:00:00

  • Inhibition of thrombin activity with DNA-aptamers.

    abstract::The effects of two DNA aptamers (oligonucleotides) 15TBA and 31TBA (15- and 31-mer thrombin-binding aptamers, respectively) on thrombin activity were studied. Both aptamers added to human plasma dose-dependently increased thrombin time (fibrin formation upon exposure to exogenous thrombin), prothrombin time (clotting ...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-009-0627-7

    authors: Dobrovolsky AB,Titaeva EV,Khaspekova SG,Spiridonova VA,Kopylov AM,Mazurov AV

    更新日期:2009-07-01 00:00:00

  • Psychoimmune interactions in women of reproductive age with endometriosis.

    abstract::Psychoimmune interactions were studied in women of reproductive age with endometriosis. Pronounced immunological shifts manifested in a shift of the T-cellular immunity, resulting in imbalanced production of pro- (IL-1β, IL-2, IFN-γ) and anti-inflammatory (IL-4) cytokines. Significant correlations between the severity...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-011-1463-0

    authors: Nasyrova RF,Sotnikova LS,Baystrukova NV,Krivoschchekova GV,Novitsky VV,Kupriyanova IE,Semke VY,Naslednikova IO,Baykov AN

    更新日期:2011-11-01 00:00:00

  • Model for alternative splicing of insulin receptor in myotonic dystrophy type 1.

    abstract::Muscular dystrophy is a common multisystem disease, which results from the impairment of alternative splicing. An increase in the number of unstable CTG and CCTG repeats in untranslated regions of the DMPK and ZNF9 genes is followed by dysregulation of RNA-binding proteins. Further changes are followed by dysfunction ...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-009-0611-2

    authors: Tonevitsky EA,Trushkin EV

    更新日期:2009-06-01 00:00:00

  • Mechanisms of defense formation against meningococcal infection in mice immunized with synthetic peptides.

    abstract::Immunization of mice with synthetic peptide fragments of conservative sites of meningococcal outer membrane proteins led to defense formation against infection with virulent serogroup A and B Meningococci. The role of cellular immunity in the formation of defense against meningococcal infection after immunization with...

    journal_title:Bulletin of experimental biology and medicine

    pub_type: 杂志文章

    doi:10.1007/s10517-007-0223-7

    authors: Chibiskova OV,Kotel'nikova OV,Nesmeyanov VA,Alliluev AP,Vol'pina OM,Koroev DO,Filatova MP

    更新日期:2007-06-01 00:00:00