Abstract：
:Hepatitis delta virus (HDV) is an RNA virus and eight clades of HDV have been identified. HDV clade 3 (HDV-3) is isolated only in the northern area of South America. The outcome of HDV-3 infection is associated with severe fulminant hepatitis. Variations in the large delta antigen (LDAg) between HDV clade 1 (HDV-1) and HDV-3 have been proposed to contribute to differences in viral secretion efficiency, but which changes might be relevant remains unclear. The control of subcellular localization of LDAg has been reported to be associated with post-translational modifications, such as phosphorylation and isoprenylation. We have observed evidence for acetylation on the LDAg of HDV-3 (LDAg-3) and LDAg of HDV-1 (LDAg-1). Green fluorescent protein-fused LDAg-3 (GFP-LD3) was used to investigate the cellular distribution and secretion of the protein. Sequence alignment of LDAg amino acids suggested that lysine-71 of LDAg-3 could be an acetylation site. Expression of a mutant form of LDAg-3 with an arginine-substitution at lysine-71 (GFP-LD3K71R) showed a distribution of the protein predominantly in the cytoplasm instead of the nucleus. Western blot analyses of secreted empty viral particles (EVPs) revealed a higher amount of secreted GFP-LD3K71R compared to GFP-LD3. Furthermore, the ectopic expression of p300, a histone acetyltransferase, led to a reduction of GFP-LD3 in EVPs. By contrast, expression of three histone deacetylases (HDAC-4, -5, and -6) facilitated the secretion of GFP-LD3. Combined, our observations support the hypothesis that the acetylation status of LDAg-3 plays a role in regulating LDAg-3's localization inside the nucleus or cytoplasm, and its secretion.
journal_name
Virus Resjournal_title
Virus researchauthors
Huang CR,Wang RY,Hsu SC,Lo SJdoi
10.1016/j.virusres.2012.08.021subject
Has Abstractpub_date
2012-12-01 00:00:00pages
75-84issue
1-2eissn
0168-1702issn
1872-7492pii
S0168-1702(12)00317-6journal_volume
170pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract：:The disulphide-linked GP2/3/4 spike of equine arteritis virus (EAV) is essential for virus entry. We showed recently that in transfected cells carbohydrates attached adjacent to the signal peptide of GP3 inhibit cleavage. Here we confirm this unique phenomenon in recombinant viruses with disabled glycosylation sites. ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.02.005
更新日期：2014-04-01 00:00:00
abstract：:MicroRNAs (miRNAs) play important roles in viral infections, especially by modulating the expression of cellular factors essential to viral replication or the host innate immune response to infection. To identify host miRNAs important to controlling porcine reproductive and respiratory syndrome virus (PRRSV) infection...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.08.012
更新日期：2015-01-02 00:00:00
abstract：:One of the leading causes of the hand, foot and mouth disease (HFMD) is Enterovirus 71 (EV-A71), displaying symptoms such as fever and ulcers in children but some strains can produce cardiopulmonary oedema which leads to death. There is no FDA-approved vaccine for prevention of severe HFMD. The molecular determinants ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2017.07.010
更新日期：2017-06-15 00:00:00
abstract：:It was previously reported that during serial passage of a BHK-derived cell line persistently infected with foot-and-mouth disease virus, (termed C1-BHK-Rc1) the virus became increasingly more virulent for BHK-21 cells (de la Torre et al 1988). Virus strains isolated from different cell passage levels were tested for ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(90)90084-o
更新日期：1990-12-01 00:00:00
abstract：:Recent X-ray crystal structure determinations of Moloney murine leukemia virus reverse transcriptase (MoMLV RT) have allowed for more accurate structure/function comparisons to HIV-1 RT than were formerly possible. Previous biochemical studies of MoMLV RT in conjunction with knowledge of sequence homologies to HIV-1 R...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2008.01.001
更新日期：2008-06-01 00:00:00
abstract：:The cDNA of the nucleocapsid (core) protein of classical swine fever virus (CSFV) was generated by reverse transcription-polymerase chain reaction (RT-PCR) and cloned into a eukaryotic expression vector. The effect of the recombinant core protein on the transcriptional regulation of cellular as well as viral promoters...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(97)00132-9
更新日期：1998-01-01 00:00:00
abstract：:We have used vesicular stomatitis virus (VSV) to determine the cost of antiserum resistance during escape from a polyclonal immune response. Replication of VSV in the presence of polyclonal antiserum resulted in the selection of antibody-escape mutants, as shown by increased fitness in the presence of antiserum and by...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2004.06.007
更新日期：2005-01-01 00:00:00
abstract：:Highly pathogenic H5N1 influenza viruses have been isolated from a number of avian and mammalian species. Despite intensive control measures the number of human and animal cases continues to increase. A more complete understanding of susceptible species and of contributing environmental and molecular factors is crucia...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2013.09.004
更新日期：2013-12-05 00:00:00
abstract：:Influenza virus is a negative strand RNA virus and is one of the rare RNA viruses to replicate in the nucleus. The viral RNA is associated with 4 viral proteins to form ribonucleoprotein particles (RNPs). After cell entry the RNPs are dissociated from the viral matrix protein in the low pH of the endosome and are acti...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2006.09.013
更新日期：2007-03-01 00:00:00
abstract：:The 2015 Brazilian Zika virus outbreak sparked a rapid response to control the spread of the virus. What was first understood to be a mild self-resolving infection is now linked to significant neurological defects in both neonates and adults. The WHO declared the 2016 Zika epidemic a public health emergency and issued...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2017.07.025
更新日期：2018-08-02 00:00:00
abstract：:In order to analyse the occurrence of viral haemorrhagic septicaemia virus (VHSV) in the marine environment surrounding Denmark, fish tissue samples were collected on four cruises with the research vessel H/S Dana in 1996 and 1997. The sampling comprised 923 samples totalling 7344 fish representing 29 different specie...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(99)00062-3
更新日期：1999-09-01 00:00:00
abstract：:Sendai virus is able to fuse with liposomes even without virus receptors. To determine the roles of envelope protein, hemagglutinin-neuraminidase (HN) and fusion (F) protein, in Sendai virus-liposome fusion, we treated the virus with proteases and examined its fusion with liposomes and the conditions of HN and F prote...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(98)00137-3
更新日期：1999-02-01 00:00:00
abstract：:Reovirus is a gastroenteric virus with a genome that consists of ten segments of double-stranded RNA. The segmented nature of the genome allows for genetic mixing when cells are simultaneously infected with two different viral serotypes. The ability of viral reassortment to take place in asynchronous infections has no...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(98)00023-9
更新日期：1998-04-01 00:00:00
abstract：:The nucleoprotein (N), phosphoprotein (P) and glycoprotein (G) genes of Irkut and West Caucasian bat viruses (WCBV) were sequenced and compared with those of other lyssaviruses. N gene nucleotide identities provided unequivocal separation of all lyssavirus genotypes with an identity threshold of 82%. On this basis, Ir...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2005.03.008
更新日期：2005-07-01 00:00:00
abstract：:Ubiquitin Specific Protease 7 (USP7) is a deubiquitinating enzyme (DUB) that plays critical roles in the regulation of many cellular processes including epigenetics, tumour suppression, oncogenesis, DNA damage response, immunity and viral infection. USP7 was the first DUB associated with viral infection. Since then ot...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2020.198076
更新日期：2020-09-01 00:00:00
abstract：:Apoptosis is an important mechanism of human immunodeficiency virus type 1 (HIV-1)-induced T-cell depletion. Our recent findings revealed mitogenic stimulation-dependent apoptosis induction in healthy donor-derived peripheral blood T-lymphocytes after adsorption with defective HIV-1 particles through acquirement by a ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(98)00052-5
更新日期：1998-07-01 00:00:00
abstract：:Two lines of LLC-MK2 cells persistently infected with human parainfluenza virus 3 (HPIV-3) have been maintained in culture for approximately 3 years. Subgenomic RNAs (putative defective interfering particle genomes) were detected in virions released from both persistently infected cultures. In one of the persistently ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(90)90039-e
更新日期：1990-04-01 00:00:00
abstract：:A wide use of inhibitors of influenza virus neuraminidase (NAIs) to control influenza in humans demands a better understanding of the mechanisms involved in the resistance emergence. In vitro studies demonstrate that both neuraminidase (NA) and hemagglutinin (HA) influence virus susceptibility to NAIs. Drug resistance...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2004.02.034
更新日期：2004-07-01 00:00:00
abstract：:Blackberry yellow vein disease (BYVD), a disorder caused by virus complexes, has become a major threat to fresh market blackberry production in the United States. Blackberry yellow vein associated virus (BYVaV) is the most prevalent virus in the BYVD complexes; detected in about 50% of samples exhibiting typical disea...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.07.009
更新日期：2012-10-01 00:00:00
abstract：:The protein and glycoprotein composition of a sucrose gradient fraction from vaccinia infected cells treated with rifampicin was studied. This particulate fraction contained cytoplasmic membranes and pleomorphic membranous structures. The glycoproteins (89, 42 and 20-23 kDa, respectively) were identified as the same g...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(90)90077-o
更新日期：1990-08-01 00:00:00
abstract：:HCV is an ideal model to study how the infected cell is altered to allow the establishment of a chronic infection. After infection, the transcriptome of the cell changes in response to the virus or to the antiviral pathways induced by infection. The cell has evolved to sense HCV soon after infection and to activate an...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2015.10.001
更新日期：2016-01-02 00:00:00
abstract：:Nineteen distinct measles virus (MV) strains associated with nine different genotypes were identified in five Australian states (Victoria, New South Wales, Queensland, Northern Territory and Western Australia) between 1999 and 2001. One of the strains identified is likely to represent a new genotype within the clade D...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(02)00273-3
更新日期：2003-02-01 00:00:00
abstract：:Viral infections are dangerous diseases for human health worldwide, which lead to significant morbidity and mortality each year. Because of their importance and the lack of effective therapeutic approaches, further attempts should be made to discover appropriate alternative or complementary treatments. Melatonin, a mu...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2020.198108
更新日期：2020-10-02 00:00:00
abstract：:Vesicular exanthema of swine virus (VESV), the prototype calicivirus, is the etiologic agent of the porcine disease vesicular exanthema of swine (VES). VES is characterized by vesicle formation on the extremities, mouth and snout and causes abortions and stillbirths if infection occurs during pregnancy. VESV is consid...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(98)00013-6
更新日期：1998-03-01 00:00:00
abstract：:Okra leaf curl disease (OLCD) is the most important viral disease of okra in West Africa. In this study, a complex of begomoviruses and associated DNA satellites were identified in symptomatic okra plants from southwestern Cameroon. Sequence analyses showed that two of the plants (Lik1 and Njo5) were infected with a b...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2013.03.010
更新日期：2013-06-01 00:00:00
abstract：:Although African swine fever virus (ASFV) replicates in viral cytoplasmic factories, the presence of viral DNA within the host cell nucleus has been previously reported to be essential for productive infection. Herein, we described, for the first time, the intranuclear distribution patterns of viral DNA replication ev...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2015.07.006
更新日期：2015-12-02 00:00:00
abstract：:The genomic RNA of vitiviruses contains 5 open reading frames (ORF). ORF3 encodes a protein to which the function of a movement protein (MP) was assigned, based on sequence homology with other viral proteins. The aim of the research described in this paper was to gain further insight in distribution profile of the ORF...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2011.10.015
更新日期：2012-01-01 00:00:00
abstract：:An orange stem pitting citrus tristeza virus (CTV) isolate CTV-B165 was found to be symptomatically similar to other known CTV-VT isolates however molecular methods failed to classify it as an identifiable CTV genotype. The sequence variation of the Indian CTV-B165 isolate was compared to the three well known CTV geno...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2010.03.017
更新日期：2010-08-01 00:00:00
abstract：:The complete nucleotide sequence has been determined for the S RNA of Aino virus, a member of the Simbu serogroup (Bunyavirus genus, family Bunyaviridae). The S RNA is 850 nucleotides long (2.76 X 10(5) daltons) and in the viral complementary sequence has a short 5' non-coding region of 34 nucleotides and a more exten...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(84)90034-0
更新日期：1984-01-01 00:00:00
abstract：:Hepatitis delta virus (HDV) requires the surface antigens of hepatitis B virus (HBV) for packaging and transmission, but replicates its RNA in an HBV-independent fashion. HDV contains a 1.7-kb circular RNA genome that is folded into an unbranched rod-like structure via intramolecular base-pairing, and possesses ribozy...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2007.01.003
更新日期：2007-08-01 00:00:00
