Abstract:
:An orange stem pitting citrus tristeza virus (CTV) isolate CTV-B165 was found to be symptomatically similar to other known CTV-VT isolates however molecular methods failed to classify it as an identifiable CTV genotype. The sequence variation of the Indian CTV-B165 isolate was compared to the three well known CTV genotypes, T36, T30, and VT. The genome of the predominant component of CTV-B165 was 19,247 nt in length with 12 open reading frames (ORFs) and was structurally identical to the other CTV isolates. All the completely sequenced CTV isolates except the VT isolate were 2-55 nt longer than the CTV-B165. In comparison to the other fully sequenced T36, T30 and VT genotypic isolates, CTV-B165 had nucleotide identity of 72-86% in ORF1 and 92-99% in ORFs 2-11. Sequence data of independent overlapping clones from the CTV-B165 genome showed highly divergent sequences of the overlapping region of 5'-UTR and ORF1a, the inter-domain region of ORF1a and the partial regions of ORF2. Phylogenetic analysis of five domains of ORF1a, ORF1b, and ORF2 revealed that CTV-B165 isolate distinctly segregates from the existing three genotypes in the dendrograms and was supported by high bootstrap values and robust tree topology. The PHYLPRO graphical analysis showed multiple recombination signals with significant correlation values. The precise detection of recombination sites for different genomic regions in CTV sequences was supported by several recombination-detecting methods. Collectively, the phylogenetic and recombination analyses suggest that the observed CTV-B165 genotype variation is an outcome of inter-genotype recombination. To determine the presence of the CTV-B165 genotype a pair of genome specific primers was designed and standardized for reliable detection of the novel CTV genotype by reverse-transcription polymerase chain reaction.
journal_name
Virus Resjournal_title
Virus researchauthors
Roy A,Brlansky RHdoi
10.1016/j.virusres.2010.03.017subject
Has Abstractpub_date
2010-08-01 00:00:00pages
118-30issue
2eissn
0168-1702issn
1872-7492pii
S0168-1702(10)00117-6journal_volume
151pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract::We have previously described the efficient homologous recombination system between 5' subgenomic RNA3a (sgRNA3a) and genomic RNA3 of Brome mosaic virus (BMV) in barley protoplasts (Sztuba-Solińska et al., 2011a). Here, we demonstrated that sequence alterations in the coat protein (CP)-binding cis-acting RNA motifs, th...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.10.001
更新日期:2012-12-01 00:00:00
abstract::Positive-strand RNA viruses exist as a quasi-species due to the incorporation of mutations into the viral genome during replication by the virus-encoded RNA-dependent RNA polymerase (RdRP). Therefore, the RdRP is often described as a low-fidelity enzyme. However, until recently, a complete description of the kinetic, ...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2004.11.004
更新日期:2005-02-01 00:00:00
abstract::Earlier studies from this laboratory have shown that infection of vascular cells with herpes simplex virus 1 or 2 (HSV-1, HSV-2) results in the differential suppression of extracellular matrix proteins including fibronectin (FN), type IV collagen, thrombospondin (TSP) and Factor VIII von Willebrand protein. The presen...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(91)90047-y
更新日期:1991-05-01 00:00:00
abstract::Since wild ducks constitute a vital element in the epizootiology of avian influenza viruses (AIVs) as well as avian paramyxoviruses (APMVs) and play a key role in the ecology and inter-species transmission of these viruses, it is crucial to elucidate the diversity and prevalence of these viruses within these bird popu...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2009.02.018
更新日期:2009-07-01 00:00:00
abstract::As agricultural acreage expanded and came to dominate landscapes across the world, viruses gained opportunities to move between crop and wild native plants. In the Midwestern USA, virus exchange currently occurs between widespread annual Poaceae crops and remnant native perennial prairie grasses now under consideratio...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2017.07.006
更新日期:2017-09-15 00:00:00
abstract:BACKGROUND:Psittacine beak and feather disease affects parrots resulting in an immunosuppressive disease that is often characterized by an abnormal shape and growth of the animal's beak, feathers, and claws. Beak and feather disease virus (BFDV) is a single-stranded circular DNA virus and is classified as a member of t...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2015.12.001
更新日期:2016-02-02 00:00:00
abstract::Swine influenza virus (SIV) is not only an important respiratory pathogen in pigs but also a threat to human health. The pandemic influenza A(H1N1)pdm09 virus likely originated in swine through reassortment between a North American triple reassortant and Eurasian avian-like SIV. The North American triple reassortant v...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2013.01.004
更新日期:2013-05-01 00:00:00
abstract::It was previously reported that during serial passage of a BHK-derived cell line persistently infected with foot-and-mouth disease virus, (termed C1-BHK-Rc1) the virus became increasingly more virulent for BHK-21 cells (de la Torre et al 1988). Virus strains isolated from different cell passage levels were tested for ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(90)90084-o
更新日期:1990-12-01 00:00:00
abstract::The extensive nucleotide sequence heterogeneity among independent genotypes of wild polioviruses permits the systematic design of genotype-specific molecular reagents. We have prepared two sets of polymerase chain reaction (PCR) primer pairs specific for the genotype of wild poliovirus type 3 recently endemic to Mexic...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(92)90124-r
更新日期:1992-08-01 00:00:00
abstract::Genome organization and gene expression of Borna disease virus (BDV) are remarkable for the overlap of open reading frames, transcription units and transcription signals, readthrough of transcription termination signals, differential use of translation initiation codons, and exploitation of the cellular splicing machi...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2009.03.016
更新日期:2009-09-01 00:00:00
abstract::Autophagy is an evolutionarily conserved lysosome-dependent degradation pathway that acts in the maintenance of cellular homeostasis and plays important functions in viral replication and pathogenesis. In this study, we investigated the role of autophagy in the replication of porcine reproductive and respiratory syndr...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2011.11.008
更新日期:2012-02-01 00:00:00
abstract::Human recombinant alpha interferon, A/D, significantly reduced the replication and cell fusion induced by herpes simplex virus type 1 in monkey cells. Thin-section electron microscopy of interferon-treated monkey cells showed distinct assembly of nucleocapsids within the nucleus. Analysis of virus-specific proteins by...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(89)90051-8
更新日期:1989-01-01 00:00:00
abstract::The fusion (F) proteins of 10 strains of bovine respiratory syncytial virus (BRSV) were compared by radioimmunoprecipitation with fractionation on SDS-polyacrylamide gels. Two different molecular weights (15 kDa and 20 kDa) of the F2 proteins were demonstrated among the BRSV strains tested. To delineate the molecular ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(93)90059-v
更新日期:1993-08-01 00:00:00
abstract::Cool-season food legumes (faba bean, lentil, chickpea and pea) and cereals (bread and durum wheat and barley) are the most important and widely cultivated crops in West Asia and North Africa (WANA), where they are the main source of carbohydrates and protein for the majority of the population. Persistently transmitted...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2008.12.007
更新日期:2009-05-01 00:00:00
abstract::In present study, we report the first complete genomic sequence of pig bovine viral diarrhea (BVD) virus, that of strain ZM-95, which is 12,220 nucleotides long and contains short 5' and 3' non-coding regions and one open reading frame encoding a large polyprotein with 28 potential N-glycosylation sites (Asn-X-Ser or ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2006.05.005
更新日期:2006-12-01 00:00:00
abstract::In order to analyse the occurrence of viral haemorrhagic septicaemia virus (VHSV) in the marine environment surrounding Denmark, fish tissue samples were collected on four cruises with the research vessel H/S Dana in 1996 and 1997. The sampling comprised 923 samples totalling 7344 fish representing 29 different specie...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(99)00062-3
更新日期:1999-09-01 00:00:00
abstract::Interferon (IFN) type-I is of utmost importance in the innate antiviral defence of eukaryotic cells. The cells express intra- and extracellular receptors that monitor their surroundings for the presence of viral genomes. Bovine viral diarrhoea virus (BVDV), a Pestivirus of the family Flaviviridae, is able to prevent I...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2008.10.015
更新日期:2009-03-01 00:00:00
abstract::At present there is much interest in the cell tropism and host range of influenza viruses, especially those of the H5N1 subtype. We wished to develop a method that would enable investigation of attachment of infectious virus through the interaction of the hemagglutinin molecule and live mammalian and avian cells and t...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2007.07.007
更新日期:2007-11-01 00:00:00
abstract::Highly pathogenic H5N1 influenza viruses have been isolated from a number of avian and mammalian species. Despite intensive control measures the number of human and animal cases continues to increase. A more complete understanding of susceptible species and of contributing environmental and molecular factors is crucia...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2013.09.004
更新日期:2013-12-05 00:00:00
abstract::A cDNA of the glycoprotein (G protein) gene of rabies virus Nishigahara strain was cloned and inserted into a baculovirus genome under the control of the polyhedrin promoter. Infection of Spodoptera frugiperda cells with this recombinant virus produced a large quantity of new protein instead of the parental polyhedrin...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(92)90095-q
更新日期:1992-09-01 00:00:00
abstract::An account is given of progress during the last 30 years in knowledge of the epidemiology of diseases caused by aphid-borne viruses in seed potato production areas of northern Europe. During this period, Potato leafroll virus (PLRV) and strains of Potato virus Y (PVY(O), PVY(N)) were consistently the most prevalent an...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/s0168-1702(00)00186-6
更新日期:2000-11-01 00:00:00
abstract::Influenza A (H1N1) virus, a high-risk infectious pathogen, can cause severe acute lung injury leading to significant morbidity and mortality. Angiotensin-converting enzyme 2 (ACE2), a negative regulator of the renin-angiotensin system (RAS), plays a protective role in pathogenesis of acute lung injury. Here, we showed...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2014.03.010
更新日期:2014-06-24 00:00:00
abstract::The purpose of this study was to test whether the region coding for subgroup envelope specificity was a determinant of oncogenic potential. An avian retrovirus recombinant was constructed to contain the envelope gene (env) determinant for subgroup A of an avian RNA tumor virus in one of the non-oncogenic, endogenous a...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(86)90048-1
更新日期:1986-11-01 00:00:00
abstract::The baculovirus late expression factor 2 (LEF-2) is involved in DNA replication, and most likely function as a primase processivity factor. Lef-2 genes have been found in multinucleocapsid nucleopolyhedroviruses (MNPVs) and in granuloviruses (GVs), but not yet in single-nucleocapsid NPV (SNPV). Here, a lef-2 gene homo...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(99)00097-0
更新日期:1999-12-01 00:00:00
abstract::Circulifer tenellus virus 1 (CiTV1) is the prototypical example of an unusual group of dsRNA viruses associated with insects and for which ecological data are lacking. A San Joaquin Valley (SJV), California population of the beet leafhopper (BLH; Circulifer tenellus [Baker]) was sampled for CiTV1 in 2010. Among 365 BL...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2013.06.017
更新日期:2013-09-01 00:00:00
abstract::Rat theilovirus (RTV) is a cardiovirus related to Theiler's murine encephalomyelitis virus. While RTV is a prevalent viral pathogen of rats used in biomedical research, the pathogenesis and characterization of RTV infections is not well understood. In the studies reported herein, we used immunohistochemistry to identi...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2011.07.014
更新日期:2011-09-01 00:00:00
abstract::The subcellular distribution of influenza polymerase PA subunit has been studied using a SV40-recombinant virus (SVPA76), which allows the expression and accumulation of this protein in COS-1 cells. In contrast to the complete nuclear localization observed for the PA subunit several hours after influenza virus infecti...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(92)90031-4
更新日期:1992-06-01 00:00:00
abstract::Theiler's murine encephalomyelitis virus is divided into two subgroups on the basis of their different biological activities. GDVII subgroup strains cause acute and fatal encephalomyelitis in mice, while TO or DA subgroup strains cause non-fatal polioencephalomyelitis in weanling mice followed by virus persistence and...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2009.11.004
更新日期:2010-02-01 00:00:00
abstract::The Sindbis viral expression system enables the rapid production of high levels of recombinant protein in mammalian cells; however, this expression is typically limited to transient production due to the cytotoxicity of the virus. Limiting the lethality inherent in the Sindbis virus vector in order to enable long term...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2008.12.019
更新日期:2009-04-01 00:00:00
abstract::In the past few decades, dengue has spread rapidly and is an emerging disease in China. An unexpected dengue outbreak occurred in Xishuangbanna, Yunnan, China, resulting in 1331 patients in 2013. In order to obtain the complete genome information and perform mutation and evolutionary analysis of causative agent relate...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2017.06.015
更新日期:2017-06-15 00:00:00