Abstract:
:We have previously described the efficient homologous recombination system between 5' subgenomic RNA3a (sgRNA3a) and genomic RNA3 of Brome mosaic virus (BMV) in barley protoplasts (Sztuba-Solińska et al., 2011a). Here, we demonstrated that sequence alterations in the coat protein (CP)-binding cis-acting RNA motifs, the Bbox region (in the intercistronic RNA3 sequence) and the RNA3 packaging element (PE, in the movement protein ORF), reduced crossover frequencies in protoplasts. Additionally, the modification of Bbox-like element in the 5' UTR region strongly debilitated crossovers. Along the lines of these observations, RNA3 mutants not expressing CP or expressing mutated CPs also reduced recombination. A series of reciprocal transfections demonstrated a functional crosstalk between the Bbox and PE elements. Altogether, our data imply the role of CP in sgRNA3a-directed recombination by either facilitating the interaction of the RNA substrates and/or by creating roadblocks for the viral replicase.
journal_name
Virus Resjournal_title
Virus researchauthors
Sztuba-Solińska J,Fanning SW,Horn JR,Bujarski JJdoi
10.1016/j.virusres.2012.10.001subject
Has Abstractpub_date
2012-12-01 00:00:00pages
138-49issue
1-2eissn
0168-1702issn
1872-7492pii
S0168-1702(12)00371-1journal_volume
170pub_type
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