Abstract:
:One of the leading causes of the hand, foot and mouth disease (HFMD) is Enterovirus 71 (EV-A71), displaying symptoms such as fever and ulcers in children but some strains can produce cardiopulmonary oedema which leads to death. There is no FDA-approved vaccine for prevention of severe HFMD. The molecular determinants of virulence for EV-A71 are unclear. It could be a single or a combination of amino acids that determines virulence in different EV-A71 genotype/sub-genotypes. Several EV-A71 strains bearing single nucleotide (nt) mutations were constructed and the contribution of each mutation to virulence was evaluated. The nt(s) that contributed to significant reduction in virulence in vitro were selected and each mutation was introduced separately into the genome to construct the multiply mutated EV-A71 strain (MMS) which carried six substitutions of nt(s) at the 5'-NTR (U700C), VP1-145 (E to G), VP1-98E, VP1-244K and G64R in the vaccine seed strain that had a partial deletion within the 5'-NTR region (nt. 475-485) of Δ11bp. In comparison to the wild type strain, the MMS showed low virulence as it produced very low RNA copy number, plaque count, VP1 and had 105-fold higher TCID50, indicative of a promising LAV candidate that should be further evaluated in vivo.
journal_name
Virus Resjournal_title
Virus researchauthors
Yee PTI,Mohamed RAH,Ong SK,Tan KO,Poh CLdoi
10.1016/j.virusres.2017.07.010subject
Has Abstractpub_date
2017-06-15 00:00:00pages
243-252eissn
0168-1702issn
1872-7492pii
S0168-1702(17)30385-4journal_volume
238pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract::HIV-1 detected among female sex workers in Calcutta, India was characterized in respect to env and nef genes. A total of 39 HIV-1 seropositive samples were used in the study. Phylogenetic analysis of the nucleotide sequences of respective regions showed that 22 out of 39 samples (56.4%) were infected with subtype C wi...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2007.06.013
更新日期:2007-12-01 00:00:00
abstract:BACKGROUND:Psittacine beak and feather disease affects parrots resulting in an immunosuppressive disease that is often characterized by an abnormal shape and growth of the animal's beak, feathers, and claws. Beak and feather disease virus (BFDV) is a single-stranded circular DNA virus and is classified as a member of t...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2015.12.001
更新日期:2016-02-02 00:00:00
abstract::Subgroup J avian leukosis virus (ALV-J) is an oncogenic retrovirus primarily causing myeloid leukosis (ML) in broilers. Although ALV is well under control in a few countries including the USA, poultry industry in many parts of the world continues suffering from serious economic loss due to sporadic or widespread ALV i...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.07.003
更新日期:2012-10-01 00:00:00
abstract::DNA clones representing the small genomic segment of Nephropathia epidemica virus strain Hällnäs B1 (NEV) and Hantaan virus strain 76-118 (HTV) encoding their nucleocapsid proteins were inserted into the E. coli vector pIN-III-ompA for secretion of proteins into the periplasmic space. The complete HTV and NEV nucleoca...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(91)90090-i
更新日期:1991-03-01 00:00:00
abstract::Infectious spleen and kidney necrosis virus (ISKNV) is the etiological agent that causes a pandemic and severe disease in fish characteristic of enlarged and damaged spleen and kidney. To identify viral proteins involved in infection and pathogenesis, we characterized five open reading frames (ORFs) of the ISKNV genom...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2006.09.003
更新日期:2007-02-01 00:00:00
abstract::Porcine deltacoronavirus (PDCoV), a newly discovered enteric coronavirus, is a causative agent of severe clinical diarrhea and intestinal pathological damage in piglets. As a first step toward understanding the effect of PDCoV on host cells, we elucidated mechanisms underlying the process of apoptotic cell death after...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2018.06.008
更新日期:2018-07-15 00:00:00
abstract::Four species of Diuris temperate terrestrial orchids from wild and captive populations were tested for the presence of polyadenylated RNA viruses. The genomes of three exotic viruses were determined: two potyviruses, Bean yellow mosaic virus and Ornithogalum mosaic virus, and the polerovirus Turnip yellows virus. The ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.10.003
更新日期:2013-01-01 00:00:00
abstract::The interaction between UV-B irradiation and cutaneous human papillomaviruses (HPV) has been suggested to be of relevance for the development of non-melanoma skin cancers. We investigated the activity within the upstream regulatory region (URR) of the HPV types 8, 38, 92, 93 and 96, as well as their responsiveness to ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2008.07.013
更新日期:2008-11-01 00:00:00
abstract::It has been previously reported that the fp25K product of Bombyx mori nucleopolyhedrovirus (BmNPV) is required for post-mortem host degradation, but the mechanism by which it regulates host degradation is still unknown. This study shows that disruption of BmNPV fp25K attenuates the expression of viral cathepsin gene (...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2008.11.014
更新日期:2009-03-01 00:00:00
abstract::The purpose of this study was to test whether the region coding for subgroup envelope specificity was a determinant of oncogenic potential. An avian retrovirus recombinant was constructed to contain the envelope gene (env) determinant for subgroup A of an avian RNA tumor virus in one of the non-oncogenic, endogenous a...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(86)90048-1
更新日期:1986-11-01 00:00:00
abstract::Noroviruses (NoVs) are a leading cause of non-bacterial acute gastroenteritis worldwide. The prevalence of Genogroup II, genotype 3 (GII.3) NoVs is secondary to the epidemic GII.4 strains which show broad spectrum binding activities against multiple types of histo-blood group antigens (HBGAs). In our previous study it...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2016.08.006
更新日期:2016-09-15 00:00:00
abstract::The glycoprotein (G) is the only surface protein of the lyssavirus particle and the only viral product known to be capable of eliciting the production of neutralizing antibodies. In this study, the isolation of escape mutants resistant to monoclonal antibody (Mab) neutralization was attempted by a selection strategy e...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2016.04.019
更新日期:2016-07-15 00:00:00
abstract::The nucleotide sequence of the matrix gene (M) of a recently identified morbillivirus, phocid distemper virus (PDV), was determined and the amino acid composition deduced. The M gene of PDV shared many characteristics with the corresponding gene in other morbilliviruses. The nucleotide homology with the closely relate...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(92)90064-g
更新日期:1992-04-01 00:00:00
abstract::A series of recombinant fusion proteins derived from equine arteritis virus (EAV) open reading frame (ORF) 7 have been used to define the immunoreactive region of the viral nucleocapsid (N) protein. Reactivities of recombinant N fusion proteins with post-infection equine sera in immunoblots and ELISAs indicate that th...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(95)00098-4
更新日期:1995-12-01 00:00:00
abstract::Seneca Valley virus (SVV) is a novel Picornaviridae that is closely associated with porcine idiopathic vesicular disease (PIVD). Here, a novel SVV strain (CH-GX-01-2019) was detected and isolated from swine in Guangxi Province, China. The complete genomic sequence of CH-GX-01-2019 exhibited 93.3-98.9 % identify with o...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2020.198177
更新日期:2021-01-02 00:00:00
abstract::An Olive latent virus 1 isolate designated GM6, obtained from a Portuguese olive tree, was characterized and the coat protein gene sequenced and analysed. The purified virus particles showed to be isometric with ca. 30 nm in diameter and contained a single-stranded RNA species with ca. 3.7 kb. The dsRNA profile obtain...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2004.09.007
更新日期:2005-03-01 00:00:00
abstract::The hemagglutinin (HA) protein of influenza virus binds to terminal sialic acid residues present on cell surface glycoproteins and glycolipids. The specific amino acids involved in this interaction have been identified for a H3 subtype HA from the human non-pathogenic virus, A/Aichi/2/68, by both crystallographic and ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(01)00407-5
更新日期:2002-02-26 00:00:00
abstract::This review describes the characterization of porcine circovirus type 2 (PCV2), the field situation of porcine circovirus-associated disease (PCVAD) and the PCV2 vaccine in Korea. PCVAD has been considered the most devastating disease in Korean livestock history since its first outbreak in 1999. Postweaning multisyste...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2011.10.013
更新日期:2012-03-01 00:00:00
abstract::Potato virus Y (PVY) is a member of the genus Potyvirus, family Potyviridae, is considered one of the most devastating pest affecting economically important crops, such as potato, tobacco, tomato and pepper, representing a serious threat due to high incidence and worldwide distribution. Its economic significance as we...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2020.198077
更新日期:2020-09-01 00:00:00
abstract::To determine the evolutionary and phylodynamic history of DENV-1 in Guangdong, the strains detected between 1985 and 2015 were determined with phylogenetic and Bayesian analyses of the E gene. Three DENV-1 genotypes (I, V, and VI) were circulating in Guangdong, and genotype I was detected most frequently. The evolutio...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2018.07.005
更新日期:2018-09-02 00:00:00
abstract::We have determined the post-translational modifications of the major capsid protein, L1 of human papillomavirus (HPV) type 6b. Since this virus cannot be cultured in the laboratory to obtain sufficient material for a study, a recombinant L1 protein produced in a vaccinia virus expression system was used in this invest...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(98)00117-8
更新日期:1999-04-01 00:00:00
abstract::Two genetic markers--the thymidine kinase gene of herpes simplex virus, and the beta-galactosidase gene of Escherichia coli--were incorporated into the 36K protein gene (IL1 gene according to the nomenclature of the Copenhagen strain of vaccinia virus; Goebel et al., 1990) from the HindIII-P DNA fragment of the LIVP s...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(93)90027-k
更新日期:1993-06-01 00:00:00
abstract::C167, a mutant derived from the XJC13 strain of Junin virus, is highly attenuated in its pathogenic properties for newborn mice. Whereas 10(2).PFU of XJC13 injected intracerebrally killed 100% of two-day-old mice, the mutant showed no detectable lethality. Survival of mice infected with C167 was associated with a redu...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(89)90074-9
更新日期:1989-08-01 00:00:00
abstract::Tomato spotted wilt virus (TSWV) is a plant-infecting member of the family Bunyaviridae. TSWV encodes two envelope glycoproteins, G(N) and G(C), which are required for virus infection of the arthropod vector. Other members of the Bunyaviridae enter host cells by pH-dependent endocytosis. During this process, the glyco...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2005.01.007
更新日期:2005-06-01 00:00:00
abstract::Real-time and visual tracking of viral infection is crucial for elucidating the infectious and pathogenesis mechanisms. To track the virus successfully, an efficient labeling method is necessary. In this review, we first discuss the practical labeling techniques for virus tracking in live cells. We then describe the c...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2017.05.007
更新日期:2017-06-02 00:00:00
abstract::Murine acquired immunodeficiency syndrome (MAIDS) induced by a defective LP-BM5 murine leukemia virus (MuLV) produces hematopoietic cytopenias similar to HIV in patients with AIDS. The pathogenesis of MAIDS induced cytopenias remains obscure; however, direct retroviral infection of bone marrow stroma has been implicat...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2005.03.027
更新日期:2005-10-01 00:00:00
abstract::The biogeographical history of rabies can be reconstructed using molecular data. This work describes the genetic characterization of the Rabies virus variant that circulates in the Desmodus rotundus (vampire bat) population in an epizootic area and is transmitted to herbivorous livestock. The N and G genes of this vir...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2009.05.001
更新日期:2009-09-01 00:00:00
abstract::Nervous necrosis virus (NNV) belongs to the genus Betanodavirus (Nodaviridae). It is highly pathogenic to various marine fishes. Here, we investigated the antigenicity changes of cultured NNV suspensions during 14days of dialyses using a dialysis tube at 1.4×10(4) molecular weight cut off (MWCO) in three different buf...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2016.07.002
更新日期:2016-09-02 00:00:00
abstract::The recent discovery of a wide range of henipavirus-like viruses circulating in Megabats in Africa raises the question as to the zoonotic potential of these pathogens given the high human mortality rates seen with their pathogenic relatives Nipah virus and Hendra virus. In the absence of cultured infectious African He...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2014.01.003
更新日期:2014-03-06 00:00:00
abstract::Three dendrimeric peptides were synthesized in order to evaluate their immunogenicity and their potential protection against classical swine fever virus (CSFV) in domestic pigs. Construct 1, an optimized version of a previously used dendrimer, had four copies of a B-cell epitope derived from CSFV E2 glycoprotein conne...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2017.05.020
更新日期:2017-06-15 00:00:00