Abstract:
:Infectious spleen and kidney necrosis virus (ISKNV) is the etiological agent that causes a pandemic and severe disease in fish characteristic of enlarged and damaged spleen and kidney. To identify viral proteins involved in infection and pathogenesis, we characterized five open reading frames (ORFs) of the ISKNV genome, ORF12, ORF65, ORF66, ORF99 and ORF111, which encode RING finger proteins (RFPs). We assessed the ubiquitin ligase (E3) activity of these recombinant RFPs fused to maltose-binding protein (MBP) using an in vitro ubiquitination assay and demonstrated that ORF12, ORF65, ORF66 and ORF111 possess the E3 activity in the presence of ubiquitin activating enzyme (E1), ubiquitin conjugating enzyme (E2), ubiquitin and zinc ion. E3 activity of ISKNV RFPs strictly depends on the UbcH5 E2 subfamily (ORF12 and ORF65 depend on UbcH5a/c, ORF66 and ORF111 depend on UbcH5a/b/c). Furthermore, point mutation in the RING domain completely abrogated ORF66 E3 activity, indicating the RING motif was essential for RFP of ISKNV. In addition, zinc ion was required as an enhancer for ISKNV RFP to exert its E3 function. Investigation of RFPs of ISKNV helps to understand their functions in the infection process and in the virus-host interaction.
journal_name
Virus Resjournal_title
Virus researchauthors
Xie J,Zhu J,Yang H,Weng S,Chen C,Chen H,Xie Q,Li M,He Jdoi
10.1016/j.virusres.2006.09.003subject
Has Abstractpub_date
2007-02-01 00:00:00pages
170-7issue
2eissn
0168-1702issn
1872-7492pii
S0168-1702(06)00275-9journal_volume
123pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract::African swine fever virus (ASFV) is the etiological agent of a contagious and often lethal disease of domestic pigs that has significant economic consequences for the swine industry. The viral genome encodes for more than 150 genes, and only a select few of these genes have been studied in some detail. Here we report ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2016.07.013
更新日期:2016-09-02 00:00:00
abstract::The interaction between UV-B irradiation and cutaneous human papillomaviruses (HPV) has been suggested to be of relevance for the development of non-melanoma skin cancers. We investigated the activity within the upstream regulatory region (URR) of the HPV types 8, 38, 92, 93 and 96, as well as their responsiveness to ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2008.07.013
更新日期:2008-11-01 00:00:00
abstract::Between 2006 and 2009, 10 field experiments were done at Kununurra, Carnarvon or Medina in Western Australia (WA) which have tropical, sub-tropical and Mediterranean climates, respectively. These experiments investigated the effectiveness of cultural control measures in limiting ZYMV spread in pumpkin, and single-gene...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2011.04.015
更新日期:2011-08-01 00:00:00
abstract::Tick-borne encephalitis virus (TBEV) strains were isolated from ticks in Western Siberia for 12 years. Molecular hybridization of the 46 viral RNA with the TBEV cDNA and oligonucleotide probes revealed differences between the Siberian and Far Eastern strains. A comparison of the viral E gene fragment nucleotide sequen...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(00)00174-x
更新日期:2000-09-01 00:00:00
abstract::The disulphide-linked GP2/3/4 spike of equine arteritis virus (EAV) is essential for virus entry. We showed recently that in transfected cells carbohydrates attached adjacent to the signal peptide of GP3 inhibit cleavage. Here we confirm this unique phenomenon in recombinant viruses with disabled glycosylation sites. ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2014.02.005
更新日期:2014-04-01 00:00:00
abstract::Equine rhinitis A virus (ERAV) is a picornavirus which causes an acute respiratory infection in horses worldwide, and virus neutralization (VN) has been the standard method for the detection of ERAV antibody in horse serum. Previous studies have identified recombinant virion protein VP1 (rVP1) purified under native co...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2009.03.003
更新日期:2009-07-01 00:00:00
abstract::We previously prepared neutralizing monoclonal antibody (MAb)-resistant (mar) mutant viruses using a laboratory strain feline infectious peritonitis virus (FIPV) 79-1146 (Kida et al., 1999). Mar mutant viruses are mutated several amino acids of the neutralizing epitope of Spike protein, compared with the parent strain...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2010.12.020
更新日期:2011-03-01 00:00:00
abstract::The rabies viruses that circulate in Arctic countries and in much of northern and central Asia are phylogenetically closely related and collectively referred to as the Arctic/Arctic-like (AL) lineage. The emergence and spread of this lineage is of significant interest given that rabies remains a serious zoonotic disea...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2011.10.026
更新日期:2012-01-01 00:00:00
abstract::Prior studies on PRRSV strain VR-2332 non-structural protein 2 (nsp2) had shown that as much as 403 amino acids could be removed from the hypervariable region without losing virus viability in vitro. We utilized selected nsp2 deletion mutants to examine in vivo growth. Young swine (4 pigs/group; 5 control swine) were ...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2010.07.024
更新日期:2010-12-01 00:00:00
abstract::The effect of mild heat shock on the replication of human cytomegalovirus (HCMV) was studied in human embryo fibroblasts. Treatment of cell cultures at 44 degrees C for 10 min just before infection or at 24 h post infection (p.i.) shortened HCMV eclipse period and enhanced viral replication, while heat shock performed...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(86)90070-5
更新日期:1986-12-01 00:00:00
abstract::Viral infections are dangerous diseases for human health worldwide, which lead to significant morbidity and mortality each year. Because of their importance and the lack of effective therapeutic approaches, further attempts should be made to discover appropriate alternative or complementary treatments. Melatonin, a mu...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2020.198108
更新日期:2020-10-02 00:00:00
abstract::Detection of Chronic bee paralysis virus (CBPV) is reported for the first time in two species of ants (Camponotus vagus and Formica rufa) and in Varroa destructor. A quantitative real-time PCR (qPCR) method was used to detect and quantify CBPV in infected bees, ants and mites. A minus-strand-specific RT-PCR was used t...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2007.12.011
更新日期:2008-05-01 00:00:00
abstract::A simple and sensitive method for the detection of measles virus genome was developed, amplifying the regions encoding the nucleocapsid (N) protein and hemagglutinin (H) protein of measles virus by reverse transcriptase-polymerase chain reaction (RT-PCR). We examined a variety of measles patients: 28 patients with nat...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(94)00074-m
更新日期:1995-01-01 00:00:00
abstract::The aim of the present study was to analyze the influence of virus origin, mammalian or mosquito cell-derived, on antiviral susceptibility of DENV-2 to entry inhibitors and the association of this effect with any alteration in the mode of entry into the cell. To this end, ten serial passages of DENV-2 were performed i...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2014.02.011
更新日期:2014-05-12 00:00:00
abstract::Pseudorabies virus (PrV), a member of herpesviridae alphaherpes subfamily, can infect human cells in vitro. However, the transmission to Old World primates including humans is strongly restricted. In this study, we report the neutralizing activity of normal human serum against PrV grown in CPK cells derived from pig. ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2003.09.008
更新日期:2004-01-01 00:00:00
abstract::Over the past three decades diseases caused by whitefly-transmitted geminiviruses (begomoviruses) have emerged to be important constraints to the production of solanaceous crops, particularly tomato (Solanum lycopersicum) and peppers (Capsicum spp.), in many tropical and subtropical regions of the world. The most stud...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2013.12.026
更新日期:2014-06-24 00:00:00
abstract::Nucleic acid probes prepared directly from bluetongue virus (BTV) genomic double-stranded RNA (dsRNA) have been used to identify the functionally equivalent genome segments from six distinct isolates of BTV after their separation in both agarose and polyacrylamide gel electrophoresis systems. Variations in the rate, a...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(88)90078-0
更新日期:1988-06-01 00:00:00
abstract::Analysis of 22 complete sequences of double-stranded DNA viruses reveals striking compositional asymmetries between leading and lagging, and between transcribed and non-transcribed strands. In all bi-directionally replicated genomes analyzed, the observed leading strand GC skew (measuring relative excess of guanines v...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(98)00139-7
更新日期:1999-03-01 00:00:00
abstract::p29, a newly identified Kaposi's sarcoma-associated herpesvirus (KSHV) protein, is the product of ORF67, the positional homolog of the conserved herpesvirus protein UL34. Like its homologues in other herpesviruses, p29 is expressed early during viral lytic cycle, and is localized on the nuclear rim. Upon chemical indu...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2013.04.001
更新日期:2013-08-01 00:00:00
abstract::DNA polymerase (DNApol) is highly conserved in baculovirus and is required for viral DNA replication. However, little is known about gammabaculovirus DNApol. Here DNApol of the gammabaculovirus Neodiprion lecontei nucleopolyhedrovirus (NeleNPV) was cloned into a dnapol-null alphabaculovirus AcMNPV bacmid, creating Bac...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2019.04.005
更新日期:2019-06-01 00:00:00
abstract::This paper characterises a virulent strain (CB/05) of canine coronavirus (CCoV) isolated from the internal organs of pups that had died of a systemic disease without evidence of other common canine pathogens. High viral RNA titres were detected in the internal organs by a real-time RT-PCR assay specific for CCoV type ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2006.12.006
更新日期:2007-04-01 00:00:00
abstract::Cold-passaged (CP) mutants derived from the JS strain of wild type wt parainfluenza type 3 virus (PIV3) are being evaluated as candidate live virus vaccines. The wt virus was serially passaged 45 times at low temperature and mutant clones with the cold-adapted (CA), temperature-sensitive (ts), and attenuation (ATT) ph...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(92)90049-f
更新日期:1992-03-01 00:00:00
abstract::The main cellular target for African swine fever virus (ASFV) is the porcine macrophage. However, existing data about the early phases of infection were previously characterized in non-leukocyte cells such as Vero cells. Here, we report that ASFV enters the natural host cell using dynamin-dependent and clathrin-mediat...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2015.01.022
更新日期:2015-03-16 00:00:00
abstract::Outbreaks caused by the highly pathogenic H5N1 avian influenza virus (A/H5N1) devastated the poultry industry in several countries and posed a significant pandemic threat. In addition to culling of infected poultry and vaccination, amantadine has been applied in poultry in some countries to control the spread of the v...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2016.11.032
更新日期:2017-01-15 00:00:00
abstract::Tmprss2 is an emerging molecular target which guides cellular infections of SARS-CoV-2, has been earmarked for interventions against the viral pathologies. The study aims to computationally screen and identifies potential miRNAs, following in vitro experimental validation of miRNA-mediated suppression of Tmprss2 for e...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2020.198275
更新日期:2021-01-08 00:00:00
abstract::The genomic RNA of vitiviruses contains 5 open reading frames (ORF). ORF3 encodes a protein to which the function of a movement protein (MP) was assigned, based on sequence homology with other viral proteins. The aim of the research described in this paper was to gain further insight in distribution profile of the ORF...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2011.10.015
更新日期:2012-01-01 00:00:00
abstract::RNA viruses exist as quasispecies containing many variants within their populations because of the error prone nature of viral RNA-dependent RNA polymerases. Quasispecies are not a simple collection of individual variants. Instead, internal interactions among variants provide quasispecies with unique evolvability. An ...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2019.02.015
更新日期:2019-04-15 00:00:00
abstract::Studies on the Marek's disease virus (MDV) serotype 2 (MDV2) genome may be important for understanding the naturally nononcogenic nature of the virus. To determine the complete DNA sequence of MDV2 unique short (Us) region, genomic BamHI fragments F, M1 and R were sequenced. The MDV2 Us region is 12109 bp long and con...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(98)00110-5
更新日期:1998-11-01 00:00:00
abstract::Viruses and virus-like particles (VLPs) of insect parasitoids modify host-parasite interactions. The Drosophila wasp, Leptopilina heterotoma, produce 300 nm spiked VLPs that bind to the host's blood cells via surface projections. L. heterotoma is a generalist wasp that attacks over a dozen Drosophila species. Oviposit...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2011.06.005
更新日期:2011-09-01 00:00:00
abstract::Circulifer tenellus virus 1 (CiTV1) is the prototypical example of an unusual group of dsRNA viruses associated with insects and for which ecological data are lacking. A San Joaquin Valley (SJV), California population of the beet leafhopper (BLH; Circulifer tenellus [Baker]) was sampled for CiTV1 in 2010. Among 365 BL...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2013.06.017
更新日期:2013-09-01 00:00:00