Changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in Vero or C6/36 cells.


:The aim of the present study was to analyze the influence of virus origin, mammalian or mosquito cell-derived, on antiviral susceptibility of DENV-2 to entry inhibitors and the association of this effect with any alteration in the mode of entry into the cell. To this end, ten serial passages of DENV-2 were performed in mosquito C6/36 cells or monkey Vero cells and the antiviral susceptibility of each virus passage to sulfated polysaccharides (SPs), like heparin and carrageenans, was evaluated by a virus plaque reduction assay. After serial passaging in Vero cells, DENV-2 became increasingly resistant to SP inhibition whereas the antiviral susceptibility was not altered in virus propagated in C6/36 cells. The change in antiviral susceptibility was associated to a differential mode of entry into the host cell. The route of endocytic entry for productive Vero cell infection was altered from a non-classical clathrin independent pathway for C6/36-grown virus to a clathrin-mediated endocytosis when the virus was serially propagated in Vero cells. Our results show the impact of the cellular system used for successive propagation of DENV on the initial interaction between the host cell and the virion in the next round of infection and the relevant consequences it might have during the in vitro evaluation of entry inhibitors.


Virus Res


Virus research


Acosta EG,Piccini LE,Talarico LB,Castilla V,Damonte EB




Has Abstract


2014-05-12 00:00:00












  • Mutations in the coat protein-binding cis-acting RNA motifs debilitate RNA recombination of Brome mosaic virus.

    abstract::We have previously described the efficient homologous recombination system between 5' subgenomic RNA3a (sgRNA3a) and genomic RNA3 of Brome mosaic virus (BMV) in barley protoplasts (Sztuba-Solińska et al., 2011a). Here, we demonstrated that sequence alterations in the coat protein (CP)-binding cis-acting RNA motifs, th...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Sztuba-Solińska J,Fanning SW,Horn JR,Bujarski JJ

    更新日期:2012-12-01 00:00:00

  • Relationships between serotypes and genotypes of hepatitis B virus: genetic classification of HBV by use of surface genes.

    abstract::Hepatitis B virus (HBV) surface antigen (HBsAg), which is encoded by the HBV S gene, is conventionally classified into 4 serological subtypes, adw, adr, ayw and ayr. To determine the relationship between the HBsAg seroreactivity and the nucleotide sequence diversity of the HBV S gene, the nucleotide sequences of S gen...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Ohba K,Mizokami M,Ohno T,Suzuki K,Orito E,Lau JY,Ina Y,Ikeo K,Gojobori T

    更新日期:1995-11-01 00:00:00

  • The impacts of viral inactivating methods on quantitative RT-PCR for COVID-19.

    abstract:OBJECTIVE:This paper aims to explore the effect of four virus inactivation methods on the rapid detection results of COVID-19 nucleic acid. METHODS:Collected samples of nasopharyngeal swabs from 2 patients diagnosed with COVID-19 at the First People's Hospital of Zhaoqing City, each of sample was divided into 5 groups...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Wang Y,Song W,Zhao Z,Chen P,Liu J,Li C

    更新日期:2020-08-01 00:00:00

  • Leader (L) of Theiler's murine encephalomyelitis virus (TMEV) is required for virus growth in a murine macrophage-like cell line.

    abstract::Theiler's murine encephalomyelitis virus is divided into two subgroups on the basis of their different biological activities. GDVII subgroup strains cause acute and fatal encephalomyelitis in mice, while TO or DA subgroup strains cause non-fatal polioencephalomyelitis in weanling mice followed by virus persistence and...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Ichinose-Asakura K,Taniura N,Himeda T,Nojiri M,Okuwa T,Ohara Y

    更新日期:2010-02-01 00:00:00

  • The complete sequences of two divergent variants of the rhabdovirus raspberry vein chlorosis virus and the design of improved primers for virus detection.

    abstract::The complete sequence was obtained for two variants of raspberry vein chlorosis virus (RVCV), confirming that this virus is a rhabdovirus most closely related to the cytorhabdoviruses alfalfa dwarf virus and strawberry crinkle virus. The two RVCV variants share only a 68% nucleotide sequence identity so that the previ...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Jones S,McGavin W,MacFarlane S

    更新日期:2019-05-01 00:00:00

  • African swine fever virus infects macrophages, the natural host cells, via clathrin- and cholesterol-dependent endocytosis.

    abstract::The main cellular target for African swine fever virus (ASFV) is the porcine macrophage. However, existing data about the early phases of infection were previously characterized in non-leukocyte cells such as Vero cells. Here, we report that ASFV enters the natural host cell using dynamin-dependent and clathrin-mediat...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Galindo I,Cuesta-Geijo MA,Hlavova K,Muñoz-Moreno R,Barrado-Gil L,Dominguez J,Alonso C

    更新日期:2015-03-16 00:00:00

  • Crop-associated virus reduces the rooting depth of non-crop perennial native grass more than non-crop-associated virus with known viral suppressor of RNA silencing (VSR).

    abstract::As agricultural acreage expanded and came to dominate landscapes across the world, viruses gained opportunities to move between crop and wild native plants. In the Midwestern USA, virus exchange currently occurs between widespread annual Poaceae crops and remnant native perennial prairie grasses now under consideratio...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Malmstrom CM,Bigelow P,Trębicki P,Busch AK,Friel C,Cole E,Abdel-Azim H,Phillippo C,Alexander HM

    更新日期:2017-09-15 00:00:00

  • Reconstitution of erythroid, megakaryocyte and myeloid hematopoietic support function with neutralizing antibodies against IL-4 and TGFbeta1 in long-term bone marrow cultures infected with LP-BM5 murine leukemia virus.

    abstract::Murine acquired immunodeficiency syndrome (MAIDS) induced by a defective LP-BM5 murine leukemia virus (MuLV) produces hematopoietic cytopenias similar to HIV in patients with AIDS. The pathogenesis of MAIDS induced cytopenias remains obscure; however, direct retroviral infection of bone marrow stroma has been implicat...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Tse KF,Inayat MS,Morrow JK,Hughes NK,Oakley OR,Gallicchio VS

    更新日期:2005-10-01 00:00:00

  • Exotic and indigenous viruses infect wild populations and captive collections of temperate terrestrial orchids (Diuris species) in Australia.

    abstract::Four species of Diuris temperate terrestrial orchids from wild and captive populations were tested for the presence of polyadenylated RNA viruses. The genomes of three exotic viruses were determined: two potyviruses, Bean yellow mosaic virus and Ornithogalum mosaic virus, and the polerovirus Turnip yellows virus. The ...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Wylie SJ,Li H,Dixon KW,Richards H,Jones MG

    更新日期:2013-01-01 00:00:00

  • Phylogenetic studies of influenza B viruses isolated in southern Africa: 1998-2001.

    abstract::Influenza B viruses isolated in southern Africa during the period from 1998 to 2001 were analysed by sequence analysis of the viral haemagglutinin HA1 subunit and the phylogenetic relationships were determined. Influenza B activity varied considerably in South Africa during the 4-year study period with no activity det...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Besselaar TG,Botha L,McAnerney JM,Schoub BD

    更新日期:2004-07-01 00:00:00

  • Cucurbit chlorotic yellows virus p22 suppressor of RNA silencing binds single-, double-stranded long and short interfering RNA molecules in vitro.

    abstract::Cucurbit chlorotic yellows virus (CCYV) is a new member of the genus Crinivirus (family Closteroviridae) with a bi-partite genome. CCYV RNA 1-encoded p22 has recently been reported to be a weak local suppressor of RNA silencing for which an interaction with cucumber SKP1LB1 through an F-box-like motif was demonstrated...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Salavert F,Navarro JA,Owen CA,Khechmar S,Pallás V,Livieratos IC

    更新日期:2020-04-02 00:00:00

  • The HBx gene of hepatitis B virus can influence hepatic microenvironment via exosomes by transferring its mRNA and protein.

    abstract::The cellular secretory vesicles known as 'exosomes' have emerged as key player in intercellular transport and communication between different eukaryotic in order to maintain body homeostasis. Many pathogenic viruses utilize exosome pathway to efficiently transfer bioactive components from infected cells to naïve cells...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Kapoor NR,Chadha R,Kumar S,Choedon T,Reddy VS,Kumar V

    更新日期:2017-08-15 00:00:00

  • Identification, sequence analysis and phylogeny of the lef-2 gene of Helicoverpa armigera single-nucleocapsid baculovirus.

    abstract::The baculovirus late expression factor 2 (LEF-2) is involved in DNA replication, and most likely function as a primase processivity factor. Lef-2 genes have been found in multinucleocapsid nucleopolyhedroviruses (MNPVs) and in granuloviruses (GVs), but not yet in single-nucleocapsid NPV (SNPV). Here, a lef-2 gene homo...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Chen X,Ijkel WF,Dominy C,de Andrade Zanotto PM,Hashimoto Y,Faktor O,Hayakawa T,Wang CH,Prekumar A,Mathavan S,Krell PJ,Hu Z,Vlak JM

    更新日期:1999-12-01 00:00:00

  • Induction of insert-specific immune response in mice by hamster polyomavirus VP1 derived virus-like particles carrying LCMV GP33 CTL epitope.

    abstract::Hamster polyomavirus (HaPyV) major capsid protein VP1 based chimeric virus-like particles (VLPs) carrying model GP33 CTL epitope derived from Lymphocytic choriomeningitis virus (LCMV) were generated in yeast and examined for their capability to induce CTL response in mice. Chimeric VP1-GP33 VLPs were effectively proce...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Mazeike E,Gedvilaite A,Blohm U

    更新日期:2012-01-01 00:00:00

  • Biological fitness and natural selection of amantadine resistant variants of avian influenza H5N1 viruses.

    abstract::Outbreaks caused by the highly pathogenic H5N1 avian influenza virus (A/H5N1) devastated the poultry industry in several countries and posed a significant pandemic threat. In addition to culling of infected poultry and vaccination, amantadine has been applied in poultry in some countries to control the spread of the v...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Abdelwhab EM,Veits J,Mettenleiter TC

    更新日期:2017-01-15 00:00:00

  • Expression cloning and antigenic analysis of the nucleocapsid protein of equine arteritis virus.

    abstract::A series of recombinant fusion proteins derived from equine arteritis virus (EAV) open reading frame (ORF) 7 have been used to define the immunoreactive region of the viral nucleocapsid (N) protein. Reactivities of recombinant N fusion proteins with post-infection equine sera in immunoblots and ELISAs indicate that th...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Chirnside ED,Francis PM,Mumford JA

    更新日期:1995-12-01 00:00:00

  • Use of pseudotyped retroviral vectors to analyze the receptor-binding pocket of hemagglutinin from a pathogenic avian influenza A virus (H7 subtype).

    abstract::The hemagglutinin (HA) protein of influenza virus binds to terminal sialic acid residues present on cell surface glycoproteins and glycolipids. The specific amino acids involved in this interaction have been identified for a H3 subtype HA from the human non-pathogenic virus, A/Aichi/2/68, by both crystallographic and ...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Lin AH,Cannon PM

    更新日期:2002-02-26 00:00:00

  • Regulation of varicella zoster virus gene 27 translation in vitro by upstream sequences.

    abstract::Northern blot analysis revealed the presence of varicella-zoster virus (VZV) gene 27 transcripts in infected cells. The Sal I-G DNA fragment, located in the unique long segment of the VZV genome and containing overlapping genes 26 and 27, was analyzed in an in vitro transcription-translation system. Translation of RNA...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Mahalingam R,Wellish M,Cabirac G,Gilden D,Vafai A

    更新日期:1988-05-01 00:00:00

  • Natural transmission and comparative analysis of small ruminant lentiviruses in the Norwegian sheep and goat populations.

    abstract::Serological surveys for small ruminant lentivirus (SRLV) infections have revealed seropositive sheep in several mixed herds, where sheep are kept together with seropositive goats. Here we have examined the genetic relationships in LTR, pol and env surface unit (SU) and the growth patterns in goat (GSM) and sheep (FOS)...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Gjerset B,Jonassen CM,Rimstad E

    更新日期:2007-05-01 00:00:00

  • Viral RNA and protein synthesis in two LLC-MK2 cell lines persistently infected with human parainfluenza virus 3.

    abstract::Two lines of LLC-MK2 cells persistently infected with human parainfluenza virus 3 (HPIV-3) have been maintained in culture for approximately 3 years. Subgenomic RNAs (putative defective interfering particle genomes) were detected in virions released from both persistently infected cultures. In one of the persistently ...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Murphy DG,Dimock K,Kang CY

    更新日期:1990-04-01 00:00:00

  • Recombinant equine herpesvirus 1 (EHV-1) vaccine protects pigs against challenge with influenza A(H1N1)pmd09.

    abstract::Swine influenza virus (SIV) is not only an important respiratory pathogen in pigs but also a threat to human health. The pandemic influenza A(H1N1)pdm09 virus likely originated in swine through reassortment between a North American triple reassortant and Eurasian avian-like SIV. The North American triple reassortant v...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Said A,Lange E,Beer M,Damiani A,Osterrieder N

    更新日期:2013-05-01 00:00:00

  • Differentiation of filoviruses by electron microscopy.

    abstract::Cultured monolayers of MA-104, Vero 76, SW-13, and DBS-FRhL-2 cells were infected with Marburg (MBG), Ebola-Sudan (EBO-S), Ebola-Zaire (EBO-Z), and Ebola-Reston (EBO-R) viruses (Filoviridae, Filovirus) and examined by electron microscopy to provide ultrastructural details of morphology and morphogenesis of these poten...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Geisbert TW,Jahrling PB

    更新日期:1995-12-01 00:00:00

  • Identification of a murine cell line that distinguishes virulent from attenuated isolates of the morbillivirus Peste des Petits Ruminants, a promising tool for virulence studies.

    abstract::Comprehensive pathogenesis studies on Peste des Petits Ruminants virus (PPRV) have been delayed so far by the absence of a small animal model reproducing the disease or an in vitro biological system revealing virulence differences. In this study, a mouse 10T1/2 cell line has been identified as presenting different sus...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Comerlato J,Albina E,Puech C,Franco AC,Minet C,Eloiflin RJ,Rodrigues V,Servan de Almeida R

    更新日期:2020-09-01 00:00:00

  • Australian monocot-infecting mastrevirus diversity rivals that in Africa.

    abstract::Monocotyledonous plant infecting mastreviruses (family Geminiviridae) have been found in the Old World. The greatest diversity of these viruses has been found in Africa but this may simply reflect the more extensive sampling that has been done there. To provide a better understanding of mastrevirus diversity in Austra...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Kraberger S,Thomas JE,Geering AD,Dayaram A,Stainton D,Hadfield J,Walters M,Parmenter KS,van Brunschot S,Collings DA,Martin DP,Varsani A

    更新日期:2012-10-01 00:00:00

  • Expression and regulation of the BKRF2, BKRF3 and BKRF4 genes of Epstein-Barr virus.

    abstract::The BKRF2, BKRF3 and BKRF4 genes of Epstein-Barr virus (EBV) are located close together in the viral genome, which encode glycoprotein L, uracil-DNA glycosylase and a tegument protein, respectively. Here, we demonstrate that the BKRF2 gene behaves as a true-late lytic gene, whereas the BKRF3 and BKRF4 genes belong to ...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Chen LW,Hung CH,Wang SS,Yen JB,Liu AC,Hung YH,Chang PJ

    更新日期:2018-09-02 00:00:00

  • RNA binding by the NS3 protease of the hepatitis C virus.

    abstract::The hepatitis C virus (HCV) nonstructural protein 3 (NS3) is essential for the processing of the HCV polyprotein, the replication of HCV RNA, and to short circuit innate immunity signaling. NS3 contains an N-terminal domain with protease activity and a C-terminal domain with helicase activity. The two domains communic...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Vaughan R,Li Y,Fan B,Ranjith-Kumar CT,Kao CC

    更新日期:2012-10-01 00:00:00

  • Porcine adenoviruses types 1, 2 and 3 have short and simple early E-3 regions.

    abstract::The nucleotide sequence of the E-3 region genes, the hexon associated protein pVIII genes, and part of the fiber genes coding for the N-terminal tail regions, of porcine adenovirus (PAV) types 1 and 2 were determined. The sizes of the E-3 regions were found to be 1162 and 1222 bp, respectively. The five open reading f...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Reddy PS,Idamakanti N,Derbyshire JB,Nagy E

    更新日期:1996-08-01 00:00:00

  • Single particle labeling of RNA virus in live cells.

    abstract::Real-time and visual tracking of viral infection is crucial for elucidating the infectious and pathogenesis mechanisms. To track the virus successfully, an efficient labeling method is necessary. In this review, we first discuss the practical labeling techniques for virus tracking in live cells. We then describe the c...

    journal_title:Virus research

    pub_type: 杂志文章,评审


    authors: Liu X,Ouyang T,Ouyang H,Ren L

    更新日期:2017-06-02 00:00:00

  • Nuclear transport of influenza virus polymerase PA protein.

    abstract::The subcellular distribution of influenza polymerase PA subunit has been studied using a SV40-recombinant virus (SVPA76), which allows the expression and accumulation of this protein in COS-1 cells. In contrast to the complete nuclear localization observed for the PA subunit several hours after influenza virus infecti...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Nieto A,de la Luna S,Bárcena J,Portela A,Valcárcel J,Melero JA,Ortín J

    更新日期:1992-06-01 00:00:00

  • Localisation of the temperature-sensitive defect in the nucleoprotein of an influenza A/FPV/Rostock/34 virus.

    abstract::The nucleotide sequences of the nucleoprotein (NP) genes of fowl plague virus (FPV) and of a temperature-sensitive (ts) mutant (ts81) derived therefrom have been determined. The ts81-NP nucleotide sequence possesses a single nucleotide substitution in comparison to the wild type. This causes an amino acid exchange at ...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Mandler J,Scholtissek C

    更新日期:1989-02-01 00:00:00