Abstract：
:Herein we describe the construction of recombinant human rhinoviruses (rHRVs) encoding HIV Gag or Tat by inserting the full length tat gene or regions of the gag gene flanked by sequences encoding the HRV 2A protease cleavage site into the junction between HRV genes encoding structural (P1) and non-structural (P2) proteins. Most recombinants were unstable, but this was corrected by mutation of the flanking cleavage sites. Thereafter, all rHRV constructs retained the inserts throughout six passages. Such constructs may find utility as vaccine vectors to generate mucosal immunity.
journal_name
Virus Resjournal_title
Virus researchauthors
Tomusange K,Yu W,Suhrbier A,Wijesundara D,Grubor-Bauk B,Gowans EJdoi
10.1016/j.virusres.2015.04.002subject
Has Abstractpub_date
2015-05-04 00:00:00pages
72-6eissn
0168-1702issn
1872-7492pii
S0168-1702(15)00136-7journal_volume
203pub_type
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