Abstract：
:We have used vesicular stomatitis virus (VSV) to determine the cost of antiserum resistance during escape from a polyclonal immune response. Replication of VSV in the presence of polyclonal antiserum resulted in the selection of antibody-escape mutants, as shown by increased fitness in the presence of antiserum and by increased resistance to neutralization. However, resistance came at a cost of overall fitness loss in the BHK-21 host cells. Sequencing of the surface G glycoprotein showed that two to four mutations were fixed in each population, most of which mapped in the A1 and A2 antigenic sites. Selected resistant populations were passaged as large populations in BHK-21 cells under constant conditions, which would normally lead to fitness increases. Nevertheless, many of the populations showed little or no sign of recovery, although the resistant phenotype was maintained. These results suggest that while antiserum resistance can develop, it may come at a cost in fitness and further limitations in the adaptability of the populations.
journal_name
Virus Resjournal_title
Virus researchauthors
Novella IS,Gilbertson DL,Borrego B,Domingo E,Holland JJdoi
10.1016/j.virusres.2004.06.007subject
Has Abstractpub_date
2005-01-01 00:00:00pages
27-34issue
1eissn
0168-1702issn
1872-7492pii
S0168-1702(04)00261-8journal_volume
107pub_type
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