Abstract：
:We have used the polymerase chain reaction (PCR) to obtain sensitive detection and identification of poliovirus RNA genomes. Primer pairs were designed to permit identification of each Sabin poliovaccine strain by the electrophoretic mobilities of the amplified DNA products (Sabin 1: 97 bp; Sabin 2: 71 bp; Sabin 3: 44 bp). The compositions of samples containing mixtures of vaccine strains could be readily determined by PCR. When the amplified products were visualized by ethidium bromide fluorescence, as few as 250 genomic copies in the original sample could be detected. When PCR was used in combination with strain-specific 32P-labeled oligonucleotide probes, the limit of detection was less than or equal to 2.5 poliovirus genomes, exceeding the sensitivity of poliovirus isolation in cell culture by at least 100-fold. PCR amplifications may be performed on virion RNAs extracted directly from clinical specimens, potentially eliminating the requirement for virus isolation in routine identifications while yielding reliable results within 8 h.
journal_name
Virus Resjournal_title
Virus researchauthors
Yang CF,De L,Holloway BP,Pallansch MA,Kew OMdoi
10.1016/0168-1702(91)90107-7subject
Has Abstractpub_date
1991-07-01 00:00:00pages
159-79issue
2eissn
0168-1702issn
1872-7492pii
0168-1702(91)90107-7journal_volume
20pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract：:A study of the evolutionary relationships of GLRaV-4,-5,-6 and -9, and two new Ampelovirus isolates (GLRaV-Pr and -De) related to grapevine leafroll disease was conducted based on molecular variability, positive selection analysis and maximum likelihood phylogenetic reconstructions. Sequences corresponding to the N-te...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.02.015
更新日期：2008-07-01 00:00:00
abstract：:We have previously described the efficient homologous recombination system between 5' subgenomic RNA3a (sgRNA3a) and genomic RNA3 of Brome mosaic virus (BMV) in barley protoplasts (Sztuba-Solińska et al., 2011a). Here, we demonstrated that sequence alterations in the coat protein (CP)-binding cis-acting RNA motifs, th...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.10.001
更新日期：2012-12-01 00:00:00
abstract：:In the present study, we show that infection with infectious bursal disease virus (IBDV) causes activation of macrophages, the key cells involved in inflammatory and immune-regulatory functions. Exposure of cultured spleen macrophages (SM) from SPF chickens to IBDV resulted in the production of nitric oxide (NO). In a...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2005.11.015
更新日期：2006-06-01 00:00:00
abstract：:PCR amplification of proviral DNA extracted from peripheral blood lymphocytes of three Chinese pigs (Banna minipig inbreed (BMI), Wu-Zhi-Shan pig (WZSP) and Neijiang pig (NJP)), using primers corresponding to highly conserved regions of reverse transcriptase (RT) of pol gene and nucleocapsid sequence of gag gene. PCR ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2004.05.007
更新日期：2004-10-01 00:00:00
abstract：:Apoptosis is frequently induced to inhibit virus replication during infection of Enterovirus 71 (EV71). On the contrary, anti-apoptotic pathway, such as PI3K/Akt pathway, is simultaneously exploited by EV71 to accomplish the viral life cycle. The relationship by which EV71-induced apoptosis and PI3K/Akt signaling path...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.07.026
更新日期：2014-11-04 00:00:00
abstract：:Post-translational modification of target proteins by the Small Ubiquitin-like Modifier (SUMO) plays a critical role in regulation of many cellular processes including transcription, RNA processing, protein trafficking, DNA repair, and chromosome segregation, and is also often hijacked by viral infections. Epstein-Bar...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2020.197962
更新日期：2020-07-02 00:00:00
abstract：:The disulphide-linked GP2/3/4 spike of equine arteritis virus (EAV) is essential for virus entry. We showed recently that in transfected cells carbohydrates attached adjacent to the signal peptide of GP3 inhibit cleavage. Here we confirm this unique phenomenon in recombinant viruses with disabled glycosylation sites. ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.02.005
更新日期：2014-04-01 00:00:00
abstract：:A series of recombinant fusion proteins derived from equine arteritis virus (EAV) open reading frame (ORF) 7 have been used to define the immunoreactive region of the viral nucleocapsid (N) protein. Reactivities of recombinant N fusion proteins with post-infection equine sera in immunoblots and ELISAs indicate that th...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(95)00098-4
更新日期：1995-12-01 00:00:00
abstract：:Puumala (PUU) viruses are the predominant etiologic agents of hantavirus infections in Europe. The most important reservoir is the bank vole, Clethrionomys glareolus (Cg), belonging to the subfamily Arvicolinae of the Muridae family. Here we report on the molecular characterization of the first rodent-derived sequence...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(99)00024-6
更新日期：1999-06-01 00:00:00
abstract：:Fourteen isolates of bovine herpesvirus 1 (BHV-1) found representative of more than 100 isolates studied, were compared by restriction fragment pattern analyses and molecularly characterized. A number of evolutionary links between the variants originally associated with infectious bovine rhinotracheitis and the varian...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(96)01383-4
更新日期：1996-12-01 00:00:00
abstract：:Several potexviruses (Family Alphaflexiviridae) have been reported infecting cassava (Manihot esculenta Crantz) in the Americas. They were isolated from severely diseased plants during the last 30-40 years and include: Cassava common mosaic virus (CsCMV), Cassava Caribbean mosaic virus (CsCaMV), Cassava Colombian symp...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2017.03.019
更新日期：2017-09-15 00:00:00
abstract：:Human respiratory syncytial virus (HRSV) P protein, 241 amino acid long, is a structural homotetrameric phosphoprotein. Viral transcription and replication processes are dependent on functional P protein interactions inside viral ribonucleoprotein complexes (RNPs). Binding capacity to RNPs proteins and transcription a...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2007.11.013
更新日期：2008-03-01 00:00:00
abstract：:Rapidly evolving RNA viruses prevail within a host as a collection of closely related variants, referred to as viral quasispecies. Advances in high-throughput sequencing (HTS) technologies have facilitated the assessment of the genetic diversity of such virus populations at an unprecedented level of detail. However, a...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2016.09.016
更新日期：2017-07-15 00:00:00
abstract：:Dromedary camel hepatitis E virus (DcHEV), a novel hepatitis E virus, has been identified in dromedary camels in Dubai, United Arab Emirates. The antigenicity, pathogenicity and epidemiology of this virus have been unclear. Here we first used a recombinant baculovirus expression system to express the 13 and 111 N-term...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2015.06.022
更新日期：2015-12-02 00:00:00
abstract：:The aim of the present study was to analyze the influence of virus origin, mammalian or mosquito cell-derived, on antiviral susceptibility of DENV-2 to entry inhibitors and the association of this effect with any alteration in the mode of entry into the cell. To this end, ten serial passages of DENV-2 were performed i...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.02.011
更新日期：2014-05-12 00:00:00
abstract：:African swine fever (ASF) is a viral hemorrhagic disease with different clinical and lesional changes depending of virulence of strains/isolates and immunological status of pigs. In acute and subacute forms of ASF, severe vascular changes are present, with hemorrhages in different organs (mainly melena, epistaxis, ery...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2013.01.017
更新日期：2013-04-01 00:00:00
abstract：:The A antigen gene from a very virulent strain of Marek's disease virus, RB-1B, has been cloned and the nucleotide sequence determined. The predicted amino acid sequence showed 99% identity to that determined for the MDV GA A antigen (Coussens and Velicer, J. Virol. 62, 2373-2379, 1988) over all but the carboxy-termin...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(89)90094-4
更新日期：1989-04-01 00:00:00
abstract：:Human metapneumovirus (hMPV) is a major cause of respiratory tract infections in children, elderly and immunocompromised hosts, for which no vaccine or treatment are currently available. Oxidative stress and inflammatory responses represent important pathogenic mechanism(s) of hMPV infection. Here, we explored the pot...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2015.01.018
更新日期：2015-03-16 00:00:00
abstract：:The family Circoviridae comprises a large group of small circular single-stranded DNA viruses with several members causing severe pig and poultry diseases. In recent years the number of new viruses within the family has had an explosive increase showing a high level of genetic diversity and a broad host range. In this...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2018.04.012
更新日期：2018-05-02 00:00:00
abstract：:In this study we demonstrated that Macrobrachium rosenbergii nodavirus (MrNV) was able to internalize and replicate in Sf9 insect cells, with levels of infection altered by substances affecting the caveolin-(CAV) mediated endocytosis pathway. The use of Sf9 cells for efficient MrNV replication and propagation was demo...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.06.012
更新日期：2016-09-02 00:00:00
abstract：:A recombinant baculovirus, the genome of which contains DNA encoding Marek's disease virus serotype 1 (MDV1) homolog of glycoprotein D (gD) of herpes simplex virus under the polyhedrin promoter was constructed and designated rAcMDV1gD. Five monoclonal antibodies (MAbs) which recognize the MDV1 homolog of gD (MDV1 gD) ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(95)00063-v
更新日期：1995-10-01 00:00:00
abstract：:A molecular epidemiological study was performed on 13 Korean virus isolates, which were collected from wild and domestic animals diagnosed as rabid between 1998 and 2004. Seven samples were from domestic animals such as dogs and cattle infected by rabid raccoon dogs (Nyctereutes procyonoides koreensis), and the rest o...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2005.06.004
更新日期：2005-12-01 00:00:00
abstract：:Papillomaviruses infect keratinocytes and their reproduction is tied to differentiation of the skin. The E2 protein of papillomaviruses is a multifunctional early protein that binds specifically to the viral DNA to regulate genome transcription, replication, and segregation. All of these are nuclear events that requir...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2010.02.011
更新日期：2010-06-01 00:00:00
abstract：:Squirrel monkey retrovirus (SMRV) is a New World primate type D retrovirus. It was shown that SMRV-related sequences could be detected in another New World species, the skunk. It was further suggested that SMRV and an Old World primate type C retrovirus, baboon endogenous virus (BaEV), may have exchanged their env gen...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(03)00178-3
更新日期：2003-10-01 00:00:00
abstract：:Japanese encephalitis virus (JEV)-specific RNAs (including 42S RNA) were synthesized in subcellular fractions prepared from infected C6/36 cells. This in vitro RNA synthesis essentially required Mg2+ and four ribonucleotides, and it was enhanced by K+. The amounts of RNA synthesized in vitro (in extracts from JEV-infe...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(89)90078-6
更新日期：1989-08-01 00:00:00
abstract：:Utilizing the reverse transcriptase-polymerase chain reaction (RT-PCR) procedure, we have synthesized full-length copies of segment 10 from African horsesickness virus (AHSV) serotypes 1, 4 and 8. The genes were cloned, sequenced and compared with the sequence of the cognate gene from AHSV serotypes 3 and 9. Sequences...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(94)90052-3
更新日期：1994-08-01 00:00:00
abstract：:Peste-des-petits-ruminants virus (PPRV) (family Paramyxoviridae, genus Morbillivirus) causes an acute febrile illness in sheep and goats resulting in significant morbidity and mortality in infected herds. The paramyxoviruses all have negative sense, non-segmented RNA genomes and their host range and pathogenic determi...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2007.01.015
更新日期：2007-06-01 00:00:00
abstract：:Human immunodeficiency virus type 1 (HIV-1) infection indirectly induces activation-dependent apoptosis in bystander immune CD4+ T-cells, a hallmark of AIDS pathogenesis. It is well known that this pathogenetic event is significantly correlated with a high virus load. Active viral replication occurs in HIV-1 asymptoma...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/s0168-1702(97)00125-1
更新日期：1997-12-01 00:00:00
abstract：:While a large number of negative-strand (-)RNA viruses infect animals and humans, a relative small number have plants as their primary host. Some of these have been classified within families together with animal/human infecting viruses due to similarities in particle morphology and genome organization, while others h...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2011.09.028
更新日期：2011-12-01 00:00:00
abstract：:White spot syndrome virus immediate early (IE) gene wsv249 encodes an E3 ubiquitin ligase that can interact with a shrimp ubiquitin-conjugating enzyme to mediate ubiquitination. In this study, to understand the transcriptional regulation of wsv249, a serial of 5'-truncated mutations were made on its promoter and the a...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2018.05.012
更新日期：2018-07-02 00:00:00
