Abstract：
:In this study we demonstrated that Macrobrachium rosenbergii nodavirus (MrNV) was able to internalize and replicate in Sf9 insect cells, with levels of infection altered by substances affecting the caveolin-(CAV) mediated endocytosis pathway. The use of Sf9 cells for efficient MrNV replication and propagation was demonstrated by confocal microscopy and PCR amplification, through which early viral binding and internalization were initially detectable at 30min post-infection; whereas at 72h, the distinguishable sign of late-MrNV infection was observable as the gradual accumulation of a cytopathic effect (CPE) in the cells, ultimately resulting in cellular disruption. Moreover, during the early period of infection, the MrNV signals were highly co-localized with CAV1 signals of the CAV-mediated endocytosis pathway. The use of genistein as an inhibitor of the CAV-mediated endocytosis pathway significantly reduced MrNV and CAV1 co-localization, and also reduced the levels of MrNV infection in Sf9 cells as shown by PCR and ELISA. Moreover, the addition of the pathway agonist okadaic acid not only recovered but also augmented both the levels of MrNV co-localization with CAV1 and of Sf9 infection in the presence of genistein inhibition; therefore demonstrating that MrNV infection in Sf9 cells was associated with the CAV-mediated endocytosis pathway machinery.
journal_name
Virus Resjournal_title
Virus researchauthors
Somrit M,Watthammawut A,Chotwiwatthanakun C,Weerachatyanukul Wdoi
10.1016/j.virusres.2016.06.012subject
Has Abstractpub_date
2016-09-02 00:00:00pages
1-9eissn
0168-1702issn
1872-7492pii
S0168-1702(16)30246-5journal_volume
223pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract：:The development of cell-mediated immunity has been known extremely important in clearing porcine reproductive and respiratory syndrome virus (PRRSV) in infected pigs. However, the PRRS immunology regarding the interaction of T-cells and PRRSV proteins is poorly understood. To identify the T-cell immunodominant epitope...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2011.03.018
更新日期：2011-06-01 00:00:00
abstract：:Raccoons (Procyon lotor), which are not native to Japan, have been suspected to transmit various pathogens by frequent intrusion into agricultural and residential areas. To determine influenza A virus seropositivity in raccoons in Japan, we examined a total of 634 raccoons captured in 19 towns (A-S) from 2009 to 2012....
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.05.004
更新日期：2014-08-30 00:00:00
abstract：:RNA-2 P75 open reading frame sequencing was conducted on BNYVV-infected sugar beet plants originating from 10 Belgian sources and was compared with sequences originating from France, Kazakhstan, Japan and China. This allowed the characterization of types A and B in Belgium. Sequence analysis confirmed that type A is i...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2004.07.007
更新日期：2005-03-01 00:00:00
abstract：:Hepatitis E is acute hepatitis caused by infection of hepatitis E virus (HEV) via a fecal-to-oral or zoonotic route. HEV is a small, non-enveloped virus containing positive strand RNA as a genome. Recently, the three-dimensional structures of the HEV-like particles and spike domain protruded from the surface of the pa...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2011.03.015
更新日期：2011-10-01 00:00:00
abstract：:Fowlpox virus (FWPV) is currently used as a vector to express foreign genes of various poultry and mammalian pathogens. However, due to limited information available about the primary structure of FWPV promoters required for an optimal transcriptional efficiency, the full potential of FWPV as an expression vector has ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2005.08.017
更新日期：2006-03-01 00:00:00
abstract：:It has previously been shown that the shortest RNA (RNA 7) of influenza C viruses codes for a nonstructural (NSI) protein (Nakada et al. (1985) J. Virol. 56, 221-226). Experiments reported here indicate that RNA 7 also directs the synthesis of a second nonstructural protein via a spliced mRNA. The amino terminal 62 co...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(86)90005-5
更新日期：1986-05-01 00:00:00
abstract：:Dengue is now hyper-endemic in most parts of south and southeast Asia including India. The northern India particularly national capital New Delhi witnessed major Dengue outbreaks with Dengue virus type 1 (DENV-1) as the dominant serotype since last five years. This study was initiated to decipher the complete genome i...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.08.018
更新日期：2015-01-02 00:00:00
abstract：:Nearly 100 years after its discovery poliovirus remains one of most thoroughly studied and best understood virus models for the molecular virologist. While poliovirus has been of vital importance for our insight into picornavirus biology at the cellular and biochemical level, it is ironic to note that, due to the earl...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2005.04.008
更新日期：2005-08-01 00:00:00
abstract：:Satellite RNAs (satRNAs) and viroids belong to the group called subviral agents and are the smallest pathogens of plants. In general, small satRNAs and viroids are 300-400 nt in size and do not encode any functional proteins; they are thus regarded as so-called long noncoding RNAs (lncRNAs). These lncRNAs are receivin...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2015.06.016
更新日期：2016-01-02 00:00:00
abstract：:Major progress in Dengue virus (DENV) biology has resulted from the use of infectious clones obtained through reverse genetics. The construction of these clones is commonly based on high- or low-copy number plasmids, yeast artificial chromosomes, yeast-Escherichia coli shuttle vectors, and bacterial artificial chromos...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2013.12.001
更新日期：2014-02-13 00:00:00
abstract：:Rhabdoviruses cause devastating diseases in aquaculture, resulting in enormous economic losses. Our previous studies indicated that imidazole arctigenin derivatives possessed antiviral activities against aquatic rhabdoviruses. Based on the data of structure-activity relationship, a new imidazole arctigenin derivative,...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2020.198019
更新日期：2020-08-01 00:00:00
abstract：:Recent X-ray crystal structure determinations of Moloney murine leukemia virus reverse transcriptase (MoMLV RT) have allowed for more accurate structure/function comparisons to HIV-1 RT than were formerly possible. Previous biochemical studies of MoMLV RT in conjunction with knowledge of sequence homologies to HIV-1 R...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2008.01.001
更新日期：2008-06-01 00:00:00
abstract：:Equine rhinitis B virus (ERBV) is the single species in the genus Erbovirus, family Picornaviridae. Equine rhinitis B viruses exist in three serotypes and are associated with respiratory disease in horses. Members of the species vary in stability at acid pH. To date there has been discordance in genotype, serotype and...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2010.12.007
更新日期：2011-02-01 00:00:00
abstract：:Cell lines were constructed which permanently express influenza virus-specific RNA. Two approaches were followed. C127 cells were transformed with bovine papilloma virus (BPV) vectors and the resulting cell lines were found to inhibit the replication of influenza virus at low multiplicity of infection (MOI 0.05). Howe...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(89)90035-x
更新日期：1989-10-01 00:00:00
abstract：:Cucurbit chlorotic yellows virus (CCYV) is a new member of the genus Crinivirus (family Closteroviridae) with a bi-partite genome. CCYV RNA 1-encoded p22 has recently been reported to be a weak local suppressor of RNA silencing for which an interaction with cucumber SKP1LB1 through an F-box-like motif was demonstrated...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2020.197887
更新日期：2020-04-02 00:00:00
abstract：:A cell-free system was developed to study the replication of simian rotavirus SA11. The components of the system included (i) subviral particles prepared from infected cells to template the synthesis of viral RNA and (ii) an mRNA-dependent rabbit reticulocyte lysate to support protein synthesis. Based upon nuclease-se...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(86)90071-7
更新日期：1986-12-01 00:00:00
abstract：:Influenza virus is a negative strand RNA virus and is one of the rare RNA viruses to replicate in the nucleus. The viral RNA is associated with 4 viral proteins to form ribonucleoprotein particles (RNPs). After cell entry the RNPs are dissociated from the viral matrix protein in the low pH of the endosome and are acti...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2006.09.013
更新日期：2007-03-01 00:00:00
abstract：:The effect of mild heat shock on the replication of human cytomegalovirus (HCMV) was studied in human embryo fibroblasts. Treatment of cell cultures at 44 degrees C for 10 min just before infection or at 24 h post infection (p.i.) shortened HCMV eclipse period and enhanced viral replication, while heat shock performed...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(86)90070-5
更新日期：1986-12-01 00:00:00
abstract：:Vaccine formulation can influence cytokine and disease patterns in mice following respiratory syncytial virus (RSV) challenge. The influence of different live and killed dual-vaccine combinations on subsequent immune responses was investigated. BALB/c mice received either killed followed by killed (KV/KV), killed foll...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2003.10.009
更新日期：2004-01-01 00:00:00
abstract：:We previously established inducible-hepatitis C virus (HCV) transgenic mice, which expressed the HCV gene (nucleotides 294-3435) encoding the core, E1, E2, and NS2 proteins. The expression of these proteins is regulated by the Cre/loxP system and an adenovirus vector (AdV) that expresses Cre DNA recombinase (Cre) cont...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2011.05.019
更新日期：2011-09-01 00:00:00
abstract：:Thogoto (THO) virus infections of Rhipicephalus appendiculatus ticks were examined using tick hosts immune to the virus. In the first set of experiments, ticks were infected by feeding on viraemic hamsters. Inter-stadial infection of THO virus was not affected when ticks ingested a virus-immune bloodmeal but there was...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(89)90034-8
更新日期：1989-10-01 00:00:00
abstract：:Severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) is recognized as one of the life-threatening viruses causing the most destructive pandemic in this century. The genesis of this virus is still unknown. To elucidate its molecular evolution and regulation of gene expression, the knowledge of codon usage is a...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2020.198113
更新日期：2020-10-15 00:00:00
abstract：:The main objective of this narrative review is to describe the available evidence on the possible antiviral activity of ozone in patients with COVID-19 and its therapeutic applicability through hospital protocols. Amongst different possible therapies for SARS-CoV-2 pneumonia, ozone therapy seems to have an immunologic...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2020.198207
更新日期：2021-01-02 00:00:00
abstract：:Murine cytomegalovirus (MCMV) m137 null mutants, Deltam137A and Deltam137B, were generated by inserting a gpt cassette into a deleted region of the open reading frame. A polyclonal antiserum produced to an Escherichia coli expressed gst-m137 fusion protein was used to show that a 38 kDa polypeptide corresponding to th...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(02)00024-2
更新日期：2002-03-20 00:00:00
abstract：:Here we document that the gene VI product of peanut chlorotic streak virus (PClSV), a newly characterized member of the group, transactivates the translation of dicistronic transcripts. Dicistronic expression units have been analyzed both in protoplast transient expression experiments and in transgenic tobacco plants....
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(98)00088-4
更新日期：1998-10-01 00:00:00
abstract：:Influenza A (H1N1) virus, a high-risk infectious pathogen, can cause severe acute lung injury leading to significant morbidity and mortality. Angiotensin-converting enzyme 2 (ACE2), a negative regulator of the renin-angiotensin system (RAS), plays a protective role in pathogenesis of acute lung injury. Here, we showed...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.03.010
更新日期：2014-06-24 00:00:00
abstract：:Purified retrovirus RD114 p30 was found to bind to the extracellular matrix proteins, fibronectin and laminin. The purified matrix proteins were immobilized onto polystyrene and the binding of p30 was quantitated using radioiodinated proteins and enzyme immunoassay (EIA). The dissociation constants were for the p30-fi...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(84)90028-5
更新日期：1984-01-01 00:00:00
abstract：:We have investigated the infection of Aedes albopictus (mosquito) cell clones by Sindbis virus. Variation in the multiplicity of infection (MOI) from ranges of 50-0.00005 pfu/cell was determined to have no effect on the progression of the infection to high acute phase titer, suggesting that intracellular factors alone...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(97)01461-5
更新日期：1997-07-01 00:00:00
abstract：:The E2 glycoprotein of classical swine fever virus (CSFV) is involved in viral infection and induction of neutralizing antibodies. Little is known about how E2 interacts with host cells. To understand the cellular factors involved in viral replication cycle, E2 was used as bait in yeast two-hybrid screens, resulting i...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2013.10.016
更新日期：2014-01-22 00:00:00
abstract：:The uptake of porcine parvovirus (PPV) into host cells, permissive or nonpermissive for PPV replication, was monitored by autoradiography, immunofluorescent microscopy, and dual parameter flow cytometry. While both permissive and nonpermissive cells selectively took up the light (activated) form of the virus from a mi...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(88)90054-8
更新日期：1988-04-01 00:00:00
