Abstract：
:RNA-2 P75 open reading frame sequencing was conducted on BNYVV-infected sugar beet plants originating from 10 Belgian sources and was compared with sequences originating from France, Kazakhstan, Japan and China. This allowed the characterization of types A and B in Belgium. Sequence analysis confirmed that type A is intermediate between types P and B. The analysis of nucleotide sequences of the capsid protein epitopes and of different motifs involved in the transmission of the virus to the plant by Polymyxa betae reveals that they are well conserved among the different sequences analysed. In silico analysis showed that a potential dimerization/dsRNA-binding domain is present on P75. Finally, in order to compare the different BNYVV types, a real-time quantitative (RT)-PCR targeting the readthrough domain of P75 was developed and assessed over a period of 40 days on BNYVV susceptible sugar beet cv. Cadyx.
journal_name
Virus Resjournal_title
Virus researchauthors
Meunier A,Schmit JF,Bragard Cdoi
10.1016/j.virusres.2004.07.007subject
Has Abstractpub_date
2005-03-01 00:00:00pages
15-22issue
1-2eissn
0168-1702issn
1872-7492pii
S0168-1702(04)00290-4journal_volume
108pub_type
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