Abstract:
:The DNA polymerase from Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) was identified and sequenced, and its amino acid sequence was compared with other viral DNA polymerases to identify conserved regions and to reconstruct a phylogenetic tree. The sequence analysis of the AgMNPV DNA polymerase gene revealed the presence of a 2976 nucleotides open reading frame (ORF) encoding a polypeptide of 991 amino acid residues with a predicted molecular mass of 114.7 kDa. Among the baculovirus DNA polymerase genes identified to date, the AgMNPV DNA polymerase gene shared maximum amino acid sequence identity with the DNA polymerase gene of Choristoneura fumiferana nucleopolyhedrovirus defective strain (CfDEFNPV) (94%). The alignment of 140 virus sequences, 23 of them from baculovirus, showed that, of the 10 conserved regions identified, 5 are exclusive to baculoviruses (R1, R5, R9, R6 and R10), only 2 of them (R6 and R10) previously described as such in the literature. Our analysis, based on their positions in the AgMNPV DNA polymerase model, suggests that R9 and R10 could interact with DNA. Phylogenetic analysis of DNA polymerase sequences places the enzyme from AgMNPV within the cluster containing the polymerases of Group I Nucleopolyhedrovirus and suggests that the AgMNPV DNA polymerase is more closely related to that of CfDEFNPV than to those of other baculoviruses.
journal_name
Virus Resjournal_title
Virus researchauthors
Dalmolin CC,da Silva FR,Mello LV,Rigden DJ,Castro MEdoi
10.1016/j.virusres.2005.01.010subject
Has Abstractpub_date
2005-06-01 00:00:00pages
99-109issue
1-2eissn
0168-1702issn
1872-7492pii
S0168-1702(05)00043-2journal_volume
110pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract::A panel of 6 neutralizing monoclonal antibodies (MAbs) raised against A/Moscow/IIV01/2009 (H1N1) virus isolated during the 2009 pandemic was used for the selection of 26 escape mutants. The mutants were characterized in immune cross-reactions with the panel of MAbs. The sequencing of the mutant HA genes revealed 5 ami...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.03.003
更新日期:2012-06-01 00:00:00
abstract::The rabies viruses that circulate in Arctic countries and in much of northern and central Asia are phylogenetically closely related and collectively referred to as the Arctic/Arctic-like (AL) lineage. The emergence and spread of this lineage is of significant interest given that rabies remains a serious zoonotic disea...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2011.10.026
更新日期:2012-01-01 00:00:00
abstract::The complete sequence was obtained for two variants of raspberry vein chlorosis virus (RVCV), confirming that this virus is a rhabdovirus most closely related to the cytorhabdoviruses alfalfa dwarf virus and strawberry crinkle virus. The two RVCV variants share only a 68% nucleotide sequence identity so that the previ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2019.03.004
更新日期:2019-05-01 00:00:00
abstract::Porcine deltacoronavirus (PDCoV), a newly discovered enteric coronavirus, is a causative agent of severe clinical diarrhea and intestinal pathological damage in piglets. As a first step toward understanding the effect of PDCoV on host cells, we elucidated mechanisms underlying the process of apoptotic cell death after...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2018.06.008
更新日期:2018-07-15 00:00:00
abstract::We here report the results of an investigation of the effect of interferon on the establishment of new infections by a retrovirus. For this study, we used an infectious but replication-incompetent retrovirus carrying a drug-resistance gene and assayed for infectivity by measuring drug-resistant colony formation. Mouse...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(89)90075-0
更新日期:1989-08-01 00:00:00
abstract::Chronic bee paralysis virus (CBPV) is responsible for chronic paralysis, an infectious and contagious disease of adult honey bees (Apis mellifera L.). The full-length nucleotide sequences of the two major RNAs of CBPV have previously been characterized. The Orf3 of RNA1 has shown significant similarities to the RNA-de...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2009.04.025
更新日期:2009-09-01 00:00:00
abstract::Adenoviruses, types 2 and 12 induce amplification of SV40 DNA sequences in cells of the SV40-transformed human newborn kidney cell line, NB-E. Similarly, integrated hepatitis B virus DNA sequences in the human hepatoma cell line, PLC/*PRF/5, and bovine papillomavirus (BPV) DNA sequences in BPV-transformed mouse cells ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(90)90079-q
更新日期:1990-08-01 00:00:00
abstract::Broad and potent human monoclonal neutralizing antibodies have considerable potential in the prevention and treatment of acquired immunodeficiency syndrome (AIDS). To identify the key amino acid recognition site contacted with neutralizing antibody Y498, peptides were panned from the PhD-12 peptide library and predict...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2020.198001
更新日期:2020-08-01 00:00:00
abstract::The hepatitis C virus (HCV) nonstructural protein 3 (NS3) is essential for the processing of the HCV polyprotein, the replication of HCV RNA, and to short circuit innate immunity signaling. NS3 contains an N-terminal domain with protease activity and a C-terminal domain with helicase activity. The two domains communic...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.07.007
更新日期:2012-10-01 00:00:00
abstract::Vesicular exanthema of swine virus (VESV), the prototype calicivirus, is the etiologic agent of the porcine disease vesicular exanthema of swine (VES). VES is characterized by vesicle formation on the extremities, mouth and snout and causes abortions and stillbirths if infection occurs during pregnancy. VESV is consid...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(98)00013-6
更新日期:1998-03-01 00:00:00
abstract::Coronaviruses are responsible for a growing economic, social and mortality burden, as the causative agent of diseases such as severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), avian infectious bronchitis virus (IBV) and COVID-19. However, there is a lack of effective antiviral agents f...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2020.197989
更新日期:2020-07-15 00:00:00
abstract::Nervous necrosis virus (NNV) belongs to the genus Betanodavirus (Nodaviridae). It is highly pathogenic to various marine fishes. Here, we investigated the antigenicity changes of cultured NNV suspensions during 14days of dialyses using a dialysis tube at 1.4×10(4) molecular weight cut off (MWCO) in three different buf...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2016.07.002
更新日期:2016-09-02 00:00:00
abstract::All entero- and rhinovirus RNAs sequenced thus far possess A and U residues at positions corresponding to nucleotides 480 and 525, respectively, of poliovirus type 1. These two nucleotides have been proposed previously to form a base pair. The single exception to this rule appears to be the Sabin type 1 strain, which ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(91)90002-d
更新日期:1991-10-01 00:00:00
abstract::Virus entry into host cells relies on interactions between viral and host structures including lipids, carbohydrates and proteins. Particularly, protein-protein interactions between viral surface proteins and host proteins as well as secondary host protein-protein interactions play a pivotal role in coordinating virus...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2015.09.006
更新日期:2016-06-15 00:00:00
abstract::The generation of a new panel of 32 monoclonal antibodies (MAbs) reactive with the P protein of the raccoon strain of rabies virus is described. Through a series of analyses employing competitive ELISA and immunoblotting, these MAbs were classified into eight groups, each defining an antigenic site, thereby increasing...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2010.06.014
更新日期:2010-09-01 00:00:00
abstract::The nucleoprotein (N), phosphoprotein (P) and glycoprotein (G) genes of Irkut and West Caucasian bat viruses (WCBV) were sequenced and compared with those of other lyssaviruses. N gene nucleotide identities provided unequivocal separation of all lyssavirus genotypes with an identity threshold of 82%. On this basis, Ir...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2005.03.008
更新日期:2005-07-01 00:00:00
abstract::From the sera of patients with advanced cancer, a novel factor called SDF (serum-derived factor) was partially purified. SDF was shown to stimulate transcription from the long terminal repeat (LTR) of human immunodeficiency virus type 1 (HIV-1) by transient CAT assay. It did not stimulate gene expression of various co...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(93)90065-u
更新日期:1993-09-01 00:00:00
abstract::During 2017, a G4P[6] group A rotavirus strain was identified in the feces of an Italian child hospitalized for acute gastroenteritis in Southern Italy. Nucleotide sequencing of the 11 genomic segments, revealed the G4-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1 genotype constellation. Phylogenetic analyses of the gene segments i...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2018.11.003
更新日期:2019-01-15 00:00:00
abstract::The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac78 gene is one of the baculovirus core genes. Recent studies showed that ac78 is essential for budded virion (BV) production and the embedding of occlusion-derived virion (ODV) into occlusion body during the AcMNPV life cycle. Here, we report that an ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2014.07.019
更新日期:2014-10-13 00:00:00
abstract::Pseudorabies virus (PrV), a member of herpesviridae alphaherpes subfamily, can infect human cells in vitro. However, the transmission to Old World primates including humans is strongly restricted. In this study, we report the neutralizing activity of normal human serum against PrV grown in CPK cells derived from pig. ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2003.09.008
更新日期:2004-01-01 00:00:00
abstract::The subcellular distribution of influenza polymerase PA subunit has been studied using a SV40-recombinant virus (SVPA76), which allows the expression and accumulation of this protein in COS-1 cells. In contrast to the complete nuclear localization observed for the PA subunit several hours after influenza virus infecti...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(92)90031-4
更新日期:1992-06-01 00:00:00
abstract::The polyprotein precursor of the Hepatitis C virus (HCV) contains multiple membrane-spanning domains that define the membrane topology and subsequent maturation of the viral structural proteins. In order to examine the biogenesis of the E1-E2 heterodimeric complex, we inserted an affinity tag (S-peptide) at specific l...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2004.04.013
更新日期:2004-09-15 00:00:00
abstract::Canine distemper virus (CDV) is a major pathogen of dogs and represents a serious threat to both unvaccinated and vaccinated animals. This study surveyed dogs with or without clinical signs related to canine distemper from different regions of Brazil from 2008 to 2012. A total of 155 out of 386 animals were found to b...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2013.12.024
更新日期:2014-02-13 00:00:00
abstract::Canine astrovirus RNA was detected in the stools of 17/63 (26.9%) samples, using either a broadly reactive consensus RT-PCR for astroviruses or random RT-PCR coupled with massive deep sequencing. The complete or nearly complete genome sequence of five canine astroviruses was reconstructed that allowed mapping the geno...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2016.12.005
更新日期:2017-03-15 00:00:00
abstract::The porcine serum inhibitor alpha 2-macroglobulin prevents influenza virus from entering host cells by competing for the SA alpha 2, 6Gal-binding site of the hemagglutinin (HA). We studied a series of inhibitor-sensitive and inhibitor-resistant human and porcine influenza virus isolates of the H3N2 subtype, all of whi...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(96)01304-4
更新日期:1996-06-01 00:00:00
abstract::Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis, a malignant B cell lymphoma. BLV has spread worldwide and causes serious problems. After infection, the BLV genome is integrated into the host DNA and can be amplified during periods of latency. We previously designed degenerate primers us...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2015.08.020
更新日期:2015-12-02 00:00:00
abstract::Mouse Hepatitis Virus (MHV) is a single-stranded positive sense RNA virus with the ability to promote acute and chronic diseases in mice. The MHV spike protein (S) is a major virulence determinant which in addition to binding to cellular receptors to mediate cell entry and facilitate virus spread to adjacent cells by ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2014.05.023
更新日期:2014-08-30 00:00:00
abstract::Although a significant progress has been achieved on dsRNA mediated anti-virus strategy development, there is still no effective means to control the virulent white spot syndrome virus (WSSV). Six double-stranded RNAs specific to different essential genes of WSSV (ie1, ie3, pol (DNA polymerase), rr2 (ribonucleotide re...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2009.06.018
更新日期:2009-10-01 00:00:00
abstract::Noncoding RNAs (ncRNAs) are key components of the transcriptome and play an important role in both normal biological activity and pathological processes such as viral infection and tumorigenesis. LncRNAs and miRNAs are the most important elements of ncRNAs and function as vital regulatory elements. Their complex regul...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2018.08.018
更新日期:2018-09-15 00:00:00
abstract::One of the leading causes of the hand, foot and mouth disease (HFMD) is Enterovirus 71 (EV-A71), displaying symptoms such as fever and ulcers in children but some strains can produce cardiopulmonary oedema which leads to death. There is no FDA-approved vaccine for prevention of severe HFMD. The molecular determinants ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2017.07.010
更新日期:2017-06-15 00:00:00