Risk factors associated with increased bovine leukemia virus proviral load in infected cattle in Japan from 2012 to 2014.


:Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis, a malignant B cell lymphoma. BLV has spread worldwide and causes serious problems. After infection, the BLV genome is integrated into the host DNA and can be amplified during periods of latency. We previously designed degenerate primers using the Coordination of Common Motifs (CoCoMo) algorithm to establish a new quantitative real-time PCR method (BLV-CoCoMo-qPCR-2) of measuring the proviral load of both known and novel BLV variants. Here, we aimed to examine the correlation between proviral load and risk factors for BLV infection, such as breeding systems, parousity, and colostrum feeding. Blood and serum samples were collected from 83 BLV-positive farms in 22 prefectures of Japan, and the BLV proviral load and anti-BLV antibody levels were measured. BLV was detected in 73.3% (1039/1,417) of cattle by BLV-CoCoMo-qPCR-2 and the provirus was detected in 93 of 1039 antibody-negative samples. The results showed that the proviral load increased with progression of lymphocytosis. Next, the risk factors associated with increasing BLV infection rate were examined along with any association with proviral load. The proviral load was higher in cattle with lymphocytosis than in healthy cattle, and higher in multiparous cows than in nulliparous cows. Finally, proviral loads were higher in contact breeding systems than in non-contact breeding systems. Taken together, these findings may help to formulate a plan for eliminating BLV from contaminated farms. This is the first nationwide study to estimate BLV proviral load in Japanese cattle.


Virus Res


Virus research


Ohno A,Takeshima SN,Matsumoto Y,Aida Y




Has Abstract


2015-12-02 00:00:00












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    journal_title:Virus research

    pub_type: 杂志文章,评审


    authors: Madhugiri R,Fricke M,Marz M,Ziebuhr J

    更新日期:2014-12-19 00:00:00

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    journal_title:Virus research

    pub_type: 杂志文章


    authors: Müller A,Markussen T,Drabløs F,Gjøen T,Jørgensen TØ,Solem ST,Mjaaland S

    更新日期:2010-08-01 00:00:00

  • Complete cDNA sequence of a South American isolate of potato virus X.

    abstract::The complete cDNA sequence corresponding to the genomic RNA of a South American strain of potato virus X (PVXc) is reported. The sequence (6432 nucleotides) contains five open reading frames coding for polypeptides with molecular weights of 165.3, 24.3, 12.3, 7.6 and 25.0 and displays an overall homology of 77.4% with...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Orman BE,Celnik RM,Mandel AM,Torres HN,Mentaberry AN

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  • Synonymous codon usage pattern analysis of Hepatitis D virus.

    abstract::Hepatitis D virus (HDV) is the smallest animal infecting RNA virus with unique features distinguishing it from other Hepatitis viruses. Codon usage variation is considered as an indicator of the forces shaping genome evolution. RSCU (relative synonymous codon usage) values, nucleotide contents, ENC (effective number o...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Bishal AK,Mukherjee R,Chakraborty C

    更新日期:2013-05-01 00:00:00

  • Demonstration of receptor binding properties of VP2 of very virulent strain infectious bursal disease virus on Vero cells.

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    journal_title:Virus research

    pub_type: 杂志文章


    authors: Yip CW,Yeung YS,Ma CM,Lam PY,Hon CC,Zeng F,Leung FC

    更新日期:2007-01-01 00:00:00

  • Microglial cells initiate vigorous yet non-protective immune responses during HSV-1 brain infection.

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    journal_title:Virus research

    pub_type: 杂志文章


    authors: Marques CP,Hu S,Sheng W,Lokensgard JR

    更新日期:2006-10-01 00:00:00

  • The peptide encoded by a novel putative lncRNA HBVPTPAP inducing the apoptosis of hepatocellular carcinoma cells by modulating JAK/STAT signaling pathways.

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    journal_title:Virus research

    pub_type: 杂志文章


    authors: Lun YZ,Pan ZP,Liu SA,Sun J,Han M,Liu B,Dong W,Pan LH,Cheng J

    更新日期:2020-10-02 00:00:00

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    journal_title:Virus research

    pub_type: 杂志文章


    authors: Patton JT

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  • Genomic and evolutionary aspects of Mimivirus.

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    journal_title:Virus research

    pub_type: 杂志文章,评审


    authors: Suzan-Monti M,La Scola B,Raoult D

    更新日期:2006-04-01 00:00:00

  • Caspase-mediated cleavage of nucleocapsid protein of a protease-independent porcine epidemic diarrhea virus strain.

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    journal_title:Virus research

    pub_type: 杂志文章


    authors: Oh C,Kim Y,Chang KO

    更新日期:2020-08-01 00:00:00

  • Characterization of the infection of Aedes albopictus cell clones by Sindbis virus.

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    journal_title:Virus research

    pub_type: 杂志文章


    authors: Karpf AR,Blake JM,Brown DT

    更新日期:1997-07-01 00:00:00

  • Nucleotide sequence of the Marek's disease virus (MDV) RB-1B A antigen gene and the identification of the MDV A antigen as the herpes simplex virus-1 glycoprotein C homologue.

    abstract::The A antigen gene from a very virulent strain of Marek's disease virus, RB-1B, has been cloned and the nucleotide sequence determined. The predicted amino acid sequence showed 99% identity to that determined for the MDV GA A antigen (Coussens and Velicer, J. Virol. 62, 2373-2379, 1988) over all but the carboxy-termin...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Binns MM,Ross NL

    更新日期:1989-04-01 00:00:00

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    abstract::By Western blot analyzes of expression of avian reovirus proteins, one unknown fragment was detected by an anti-sigmaA monoclonal antibody in virus-infected cells lysate. It was interesting to note that RNA interference against sigmaA resulted in the suppression of the unknown fragment. Using various lengths of sigmaA...

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    pub_type: 杂志文章


    authors: Ji WT,Lin FL,Wang YC,Shih WL,Lee LH,Liu HJ

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    journal_title:Virus research

    pub_type: 杂志文章


    authors: Liu Y,Joo KI,Lei Y,Wang P

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    pub_type: 杂志文章


    authors: Wylie SJ,Li H,Dixon KW,Richards H,Jones MG

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    pub_type: 杂志文章


    authors: Maliogka VI,Dovas CI,Katis NI

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    pub_type: 杂志文章


    authors: Lokugamage K,Kariwa H,Lokugamage N,Miyamoto H,Iwasa M,Hagiya T,Araki K,Tachi A,Mizutani T,Yoshimatsu K,Arikawa J,Takashima I

    更新日期:2004-05-01 00:00:00

  • Sequence diversity and potential recombination events in the coat protein gene of Apple stem pitting virus.

    abstract::The variability of the Apple stem pitting virus (ASPV) coat protein (CP) gene was investigated. The CP gene of ten virus isolates from apple and pear trees was sequenced. Comparison of all sequenced virus isolates revealed high diversity of the CP gene (70.7-93.5% at the nucleotide level and 77.8-98.7% at the amino ac...

    journal_title:Virus research

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    authors: Komorowska B,Siedlecki P,Kaczanowski S,Hasiów-Jaroszewska B,Malinowski T

    更新日期:2011-06-01 00:00:00

  • Induction of insert-specific immune response in mice by hamster polyomavirus VP1 derived virus-like particles carrying LCMV GP33 CTL epitope.

    abstract::Hamster polyomavirus (HaPyV) major capsid protein VP1 based chimeric virus-like particles (VLPs) carrying model GP33 CTL epitope derived from Lymphocytic choriomeningitis virus (LCMV) were generated in yeast and examined for their capability to induce CTL response in mice. Chimeric VP1-GP33 VLPs were effectively proce...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Mazeike E,Gedvilaite A,Blohm U

    更新日期:2012-01-01 00:00:00

  • Genetic and phylogenetic analysis of the core proteins VP1, VP3, VP4, VP6 and VP7 of epizootic haemorrhagic disease virus (EHDV).

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    journal_title:Virus research

    pub_type: 杂志文章


    authors: Anthony SJ,Maan N,Maan S,Sutton G,Attoui H,Mertens PP

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    abstract::Viruses frequently spread among cells or hosts in groups, with multiple viral genomes inside the same infectious unit. These collective infectious units can consist of multiple viral genomes inside the same virion, or multiple virions inside a larger structure such as a vesicle. Collective infectious units deliver mul...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Leeks A,Sanjuán R,West SA

    更新日期:2019-05-01 00:00:00

  • Development of a mandarin fish Siniperca chuatsi fry cell line suitable for the study of infectious spleen and kidney necrosis virus (ISKNV).

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    journal_title:Virus research

    pub_type: 杂志文章


    authors: Dong C,Weng S,Shi X,Xu X,Shi N,He J

    更新日期:2008-08-01 00:00:00

  • Bacterial viruses against viruses pathogenic for man?

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    journal_title:Virus research

    pub_type: 杂志文章,评审


    authors: Miedzybrodzki R,Fortuna W,Weber-Dabrowska B,Gorski A

    更新日期:2005-06-01 00:00:00

  • Population structure of Blackberry yellow vein associated virus, an emerging crinivirus.

    abstract::Blackberry yellow vein disease (BYVD), a disorder caused by virus complexes, has become a major threat to fresh market blackberry production in the United States. Blackberry yellow vein associated virus (BYVaV) is the most prevalent virus in the BYVD complexes; detected in about 50% of samples exhibiting typical disea...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Poudel B,Sabanadzovic S,Bujarski J,Tzanetakis IE

    更新日期:2012-10-01 00:00:00

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    journal_title:Virus research

    pub_type: 杂志文章


    authors: de Mattos CA,de Mattos CC,Osburn BI,MacLachlan NJ

    更新日期:1994-01-01 00:00:00

  • An improved in vitro and in vivo Sindbis virus expression system through host and virus engineering.

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    journal_title:Virus research

    pub_type: 杂志文章


    authors: Nivitchanyong T,Tsai YC,Betenbaugh MJ,Oyler GA

    更新日期:2009-04-01 00:00:00

  • Adenovirus infection induces amplification of persistent viral DNA sequences (simian virus 40, hepatitis B virus, bovine papillomavirus) in human and rodent cells.

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    journal_title:Virus research

    pub_type: 杂志文章


    authors: Schlehofer JR,zur Hausen H

    更新日期:1990-08-01 00:00:00

  • Early intranuclear replication of African swine fever virus genome modifies the landscape of the host cell nucleus.

    abstract::Although African swine fever virus (ASFV) replicates in viral cytoplasmic factories, the presence of viral DNA within the host cell nucleus has been previously reported to be essential for productive infection. Herein, we described, for the first time, the intranuclear distribution patterns of viral DNA replication ev...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Simões M,Martins C,Ferreira F

    更新日期:2015-12-02 00:00:00

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    journal_title:Virus research

    pub_type: 杂志文章


    authors: Chiu CN,Mitra R,Chiu IM

    更新日期:2003-10-01 00:00:00

  • Preparation and characterization of monoclonal antibodies to nucleocapsid protein N and H glycoprotein of rinderpest virus.

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    journal_title:Virus research

    pub_type: 杂志文章


    authors: Bhavani K,Karande AA,Shaila MS

    更新日期:1989-04-01 00:00:00