Abstract：
:To identify the parts of retroviral integrase that interact with its DNA substrates, we compared the patterns of target site usage by chimeric enzymes and protein fragments in assays that reveal integrase's non-specific nuclease activities. The central region of 12 chimeric proteins between the human immunodeficiency virus type 1 and visna virus integrases was found to be responsible for selecting non-viral target DNA sites when small alcohols provide the attacking nucleophilic OH group during non-specific alcoholysis assays. Testing deletion derivatives of the integrase protein in this assay, which has similarities to the DNA joining reaction that occurs during retroviral integration, defined a smaller central domain that is sufficient for activity. Thus, this core domain likely contains both the host DNA site and the nucleophile site. Surprisingly, the region of integrase responsible for selecting non-viral target DNA sites when the viral DNA end is the attacking nucleophile could not similarly be mapped with the standard oligonucleotide joining assay. We therefore tested the proteins in a more sensitive assay that displays preferred sites of viral DNA insertion in a plasmid DNA target. All 12 chimeras yielded novel patterns compared with the wild-type enzymes in this assay, although local insertion patterns indicated that the central domain plays an important role in target site selection. Together, these data suggest that other protein regions must be involved when the attacking nucleophilic group is provided by viral DNA. Because specific recognition of viral DNA ends was previously mapped to the central domain, two different regions of integrase must interact with retroviral DNA.
journal_name
Virus Resjournal_title
Virus researchauthors
Katzman M,Sudol M,Pufnock JS,Zeto S,Skinner LMdoi
10.1016/s0168-1702(99)00126-4subject
Has Abstractpub_date
2000-01-01 00:00:00pages
87-100issue
1eissn
0168-1702issn
1872-7492pii
S0168-1702(99)00126-4journal_volume
66pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract：:MicroRNAs (miRNAs) constitute an important class of non-coding RNA implicated in gene expression regulation. More than 1900 miRNA molecules have been identified in humans and their modulation during viral infection and it is recognized to play a role in latency regulation or in establishing an antiviral state. The liv...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.11.010
更新日期：2015-01-22 00:00:00
abstract：:While large-scale dog vaccination campaigns have significantly reduced urban rabies throughout Mexico, reports of sylvatic rabies, including cases of spill-over of bat strains into livestock and humans, are increasing. To improve knowledge of these epidemiological trends, 64 Mexican rabies virus isolates from various ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2005.10.006
更新日期：2006-05-01 00:00:00
abstract：:The nucleocapsids formation is a pivotal step of hepatitis B virus (HBV) life cycle. The inhibition of HBV nucleocapsids assembly is a promising strategy for the anti-HBV treatment. HBc78-117 is an internal fragment of hepatitis B core protein (HBc). In this study, we used lentiviral vector to deliver HBc78-117 cDNA s...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2010.03.005
更新日期：2010-06-01 00:00:00
abstract：:A variant clone of Snyder-Theilen feline sarcoma virus (ST-FeSV) encoding a polyprotein with a molecular weight of approximately 104 kDa (P104) was compared to the P85 encoding prototype clone of ST-FeSV. Analysis of chimeric genes constructed with the viral oncogenes of the two clones indicated that the variant clone...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(87)90007-4
更新日期：1987-11-01 00:00:00
abstract：:The entire genome of a mid-Atlantic raccoon strain rabies virus (RRV) isolated in Canada was sequenced; this is the second North American wildlife rabies virus isolate to be fully characterized. The overall organization and length of the genome was similar to that of other lyssaviruses. The nucleotide sequence identit...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.04.029
更新日期：2008-09-01 00:00:00
abstract：:Virus entry into host cells relies on interactions between viral and host structures including lipids, carbohydrates and proteins. Particularly, protein-protein interactions between viral surface proteins and host proteins as well as secondary host protein-protein interactions play a pivotal role in coordinating virus...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2015.09.006
更新日期：2016-06-15 00:00:00
abstract：:Quasispecies dynamics mediates adaptability of RNA viruses through a number of mechanisms reviewed in the present article, with emphasis on the medical implications for the hepatitis viruses. We discuss replicative and non-replicative molecular mechanisms of genome variation, modulating effects of mutant spectra, and ...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2007.02.001
更新日期：2007-08-01 00:00:00
abstract：:Coronaviruses are a large group of enveloped, single-stranded positive-sense RNA viruses that infect a wide range of avian and mammalian species, including humans. The emergence of deadly human coronaviruses, severe acute respiratory syndrome coronavirus (SARS-CoV), and Middle East respiratory syndrome coronavirus (ME...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2014.11.021
更新日期：2015-04-16 00:00:00
abstract：:A disease of Rudbeckia hirta (Black-eyed Susan), characterized by severe flower deformation, was observed in Minnesota during 2010-2016. A previously undescribed virus species, named Rudbeckia flower distortion virus (RuFDV, family Caulimoviridae, genus unassigned), was determined to be the causal agent of the disease...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2017.05.012
更新日期：2017-09-15 00:00:00
abstract：:Ranaviruses (family Iridoviridae, genus Ranavirus) have been recognized as emerging infectious pathogens and caused a great loss to the global biodiversity. Thymidine kinase (TK) and deoxyuridine triphosphatase (dUTPase, DUT, encoded by ORF 67R) are ubiquitous, existing in iridoviruses and other organisms. Previous st...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.01.008
更新日期：2016-03-02 00:00:00
abstract：:Helminthosporium victoriae virus 190S (HvV190S) is the type species of the genus Victorivirus under the family Totiviridae. To date, HvV190S has never been found in places outside of the USA and has Helminthosporium victoriae as its only know natural host fungus in the field. Here, we report the identification of 4 do...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2020.197941
更新日期：2020-08-01 00:00:00
abstract：:Sixty-six human enterovirus serotypes have been described using antibody neutralization, with antigenic variants defined within several serotypes. Despite the availability of sequence data for numerous enteroviruses, the molecular basis of serotype is unknown. Previous studies by others have identified four major phyl...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(98)00101-4
更新日期：1998-11-01 00:00:00
abstract：:The epitopes of six monoclonal antibodies generated against type A12 foot-and-mouth disease virus (FMDV) VP1 or its largest cyanogen bromide fragment (13 kd) were characterized. Five of these monoclonal antibodies neutralized viral infectivity. Solid-phase and competitive antigen binding assays using virion-derived an...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(84)90006-6
更新日期：1984-09-01 00:00:00
abstract：:The genomic RNA of vitiviruses contains 5 open reading frames (ORF). ORF3 encodes a protein to which the function of a movement protein (MP) was assigned, based on sequence homology with other viral proteins. The aim of the research described in this paper was to gain further insight in distribution profile of the ORF...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2011.10.015
更新日期：2012-01-01 00:00:00
abstract：:HIV-1 detected among female sex workers in Calcutta, India was characterized in respect to env and nef genes. A total of 39 HIV-1 seropositive samples were used in the study. Phylogenetic analysis of the nucleotide sequences of respective regions showed that 22 out of 39 samples (56.4%) were infected with subtype C wi...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2007.06.013
更新日期：2007-12-01 00:00:00
abstract：:In lamivudine-refractory chronic hepatitis B (CHB) patients, discontinuation of lamivudine therapy may lead to loss of lamivudine-resistant hepatitis B virus (HBV) and reappearance of wide-type HBV as dominant strains, yet the underlying mechanism remains unclear. In this study, we cloned wide-type and lamivudine-resi...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2018.07.021
更新日期：2018-08-15 00:00:00
abstract：:Vesicular exanthema of swine virus (VESV), the prototype calicivirus, is the etiologic agent of the porcine disease vesicular exanthema of swine (VES). VES is characterized by vesicle formation on the extremities, mouth and snout and causes abortions and stillbirths if infection occurs during pregnancy. VESV is consid...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(98)00013-6
更新日期：1998-03-01 00:00:00
abstract：:Persistent infections with mumps virus were established in human B-lymphoid cell line Akata and in the human chronic myelogenous leukaemia cell line K562. Even after IFN treatment a drastic decrease in STAT-1alpha (signal transducers and activators of transcription-1alpha), STAT-2 and p48 (ISGF-3gamma: IFN-stimulated ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(99)00114-8
更新日期：1999-12-15 00:00:00
abstract：:Entomopoxviruses are an important group of viruses infecting only insects. They belong to Poxviridae which infect both invertebrates and vertebrates, including humans. Protein kinases are known to have roles at virus morphogenesis, host selectivity, the regulation of cell division and apoptosis in some vertebrate poxv...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2018.02.007
更新日期：2018-03-15 00:00:00
abstract：:Here we document that the gene VI product of peanut chlorotic streak virus (PClSV), a newly characterized member of the group, transactivates the translation of dicistronic transcripts. Dicistronic expression units have been analyzed both in protoplast transient expression experiments and in transgenic tobacco plants....
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(98)00088-4
更新日期：1998-10-01 00:00:00
abstract：:Viral vectors are important vehicles in vaccine research. Avipoxviruses including fowlpox virus (FPV) play major roles in viral vaccine vector development for the prevention and therapy of human and other veterinary diseases due to their immunomodulatory effects and safety profile. Recently, we analyzed the genomic an...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.12.015
更新日期：2015-02-02 00:00:00
abstract：:The human respiratory coronavirus OC43 was grown on a human rectal tumor cell line and was isotopically labeled with amino acids, glucosamine, and orthophosphate to analyze virion structural proteins. Four major protein species were resolved by electrophoresis and many of their properties were deduced from digestion s...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(86)90013-4
更新日期：1986-08-01 00:00:00
abstract：:It has recently been reported that the xenotropic murine leukemia virus-related virus (XMRV) derives from a laboratory recombinant. However, sequences with characteristics of the 5' half of XMRV (termed PreXMRV-2) have been identified in several laboratory mouse genomes and cell lines suggesting parts of the XMRV geno...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.06.026
更新日期：2012-10-01 00:00:00
abstract：:Infectious spleen and kidney necrosis virus (ISKNV) is the etiological agent that causes a pandemic and severe disease in fish characteristic of enlarged and damaged spleen and kidney. To identify viral proteins involved in infection and pathogenesis, we characterized five open reading frames (ORFs) of the ISKNV genom...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2006.09.003
更新日期：2007-02-01 00:00:00
abstract：:Two lines of LLC-MK2 cells persistently infected with human parainfluenza virus 3 (HPIV-3) have been maintained in culture for approximately 3 years. Subgenomic RNAs (putative defective interfering particle genomes) were detected in virions released from both persistently infected cultures. In one of the persistently ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(90)90039-e
更新日期：1990-04-01 00:00:00
abstract：:MicroRNAs play vital roles in regulating the battle between pathogens and host cells during viral challenging. MiR-4331 aggravates transmissible gastroenteritis virus (TGEV) -induced mitochondrial damage, also suppresses transcription of TGEV gene 7 via targeting cellular CDCA7. Otherwise, miR-4331-5p affects H1N1/200...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2020.198064
更新日期：2020-09-01 00:00:00
abstract：:A prospective, cross-sectional study was conducted to investigate the correlation between the integration of high-risk human papillomavirus and disease severity of cervical lesions. 720 liquid-based cytology specimens including 422 normal cytology, 78 low-grade squamous intraepithelial lesions, 172 high-grade squamous...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2011.06.012
更新日期：2011-11-01 00:00:00
abstract：:Severe Combined Immune Deficiency mouse tumors, induced by inoculating peripheral blood mononuclear cells from 11 healthy human donors (hu-PBMC-SCID tumors), were used to analyse Epstein-Barr virus (EBV) type and strain variations. PCR analysis of EBNA 2- and EBNA 3C-specific sequences showed that EBV type A was prese...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(95)00005-b
更新日期：1995-05-01 00:00:00
abstract：:The entry of herpes simplex virus (HSV) into an oral epithelial cell line, primary normal human oral keratinocytes (NHOK) and gingival fibroblasts (GF) was examined. Infection of these cells by HSV-1 and HSV-2 was blocked by heparin. Further examination indicated that heparin reduced viral attachment but not penetrati...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(02)00055-2
更新日期：2002-06-01 00:00:00
abstract：:The packaging of HIV genomic RNA is mediated by a specific interaction between a nucleocapsid (NC) protein and packaging signal (psi, psi) RNA sequence. However, this interaction can be inhibited by the presence of peptides or proteins that specifically bind to the psi sequence. The 125-base-long psi RNA comprises a s...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2004.05.012
更新日期：2004-11-01 00:00:00
