Abstract:
:Here we document that the gene VI product of peanut chlorotic streak virus (PClSV), a newly characterized member of the group, transactivates the translation of dicistronic transcripts. Dicistronic expression units have been analyzed both in protoplast transient expression experiments and in transgenic tobacco plants. Transgenic plants containing a dicistronic transcription unit (PClSV-gene VII-GUS) under the control of PClSV full-length transcript promoter with its long leader sequence show a relatively high abundance of the expected transcript but very little, or no, GUS activity. However, high GUS activity is found when gene VI protein is then provided by subsequent infection with PClSV. The efficient translation of polycistronic mRNAs mediated by gene VI of caulimovirus has potential value in product engineering of plants.
journal_name
Virus Resjournal_title
Virus researchauthors
Maiti IB,Richins RD,Shepherd RJdoi
10.1016/s0168-1702(98)00088-4subject
Has Abstractpub_date
1998-10-01 00:00:00pages
113-24issue
2eissn
0168-1702issn
1872-7492pii
S0168170298000884journal_volume
57pub_type
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