Abstract：
:Hantaviruses are the etiologic agents of Hemorrhagic Fever with Renal Syndrome (HFRS) in Old World, and Hantavirus Pulmonary Syndrome (HPS)/Hantavirus Cardiopulmonary Syndrome (HCPS), in the New World. Serological methods are the most common approach used for laboratory diagnosis of HCPS, however theses methods do not allow the characterization of viral genotypes. The polymerase chain reaction (PCR) has been extensively used for diagnosis of viral infections, including those caused by hantaviruses, enabling detection of few target sequence copies in the sample. However, most studies proposed methods of PCR with species-specific primers. This study developed a simple and reliable diagnostic system by RT-PCR for different hantavirus detection. Using new primers set, we evaluated human and rodent hantavirus positive samples of various regions from Brazil. Besides, we performed computational analyzes to evaluate the detection of other South American hantaviruses. The diagnostic system by PCR proved to be a sensible and simple assay, allowing amplification of Juquitiba virus, Araraquara virus, Laguna Negra virus, Rio Mamore virus and Jabora virus, beyond of the possibility of the detecting Andes, Anajatuba, Bermejo, Choclo, Cano Delgadito, Lechiguanas, Maciel, Oran, Pergamino and Rio Mearim viruses. The primers sets designed in this study can detect hantaviruses from almost all known genetics lineages in Brazil and from others South America countries and also increases the possibility to detect new hantaviruses. These primers could easily be used both in diagnosis of suspected hantavirus infections in humans and also in studies with animals reservoirs.
journal_name
Virus Resjournal_title
Virus researchauthors
Guterres A,de Oliveira RC,Fernandes J,Schrago CG,de Lemos ERdoi
10.1016/j.virusres.2015.07.022subject
Has Abstractpub_date
2015-12-02 00:00:00pages
106-13eissn
0168-1702issn
1872-7492pii
S0168-1702(15)30026-5journal_volume
210pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract：:Apoptosis is frequently induced to inhibit virus replication during infection of Enterovirus 71 (EV71). On the contrary, anti-apoptotic pathway, such as PI3K/Akt pathway, is simultaneously exploited by EV71 to accomplish the viral life cycle. The relationship by which EV71-induced apoptosis and PI3K/Akt signaling path...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.07.026
更新日期：2014-11-04 00:00:00
abstract：:Positive-strand RNA viruses exist as a quasi-species due to the incorporation of mutations into the viral genome during replication by the virus-encoded RNA-dependent RNA polymerase (RdRP). Therefore, the RdRP is often described as a low-fidelity enzyme. However, until recently, a complete description of the kinetic, ...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2004.11.004
更新日期：2005-02-01 00:00:00
abstract：:Hepatitis E virus (HEV) causes acute or chronic hepatitis in humans and can be transmitted via the fecal-oral route. Pigs are one of the main reservoirs for this infection. Sixty pigs, 4-5 months of age, on a swine herd in Japan had detectable anti-HEV IgG antibodies, and five (8.3%) of them had ongoing infection of g...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2017.08.003
更新日期：2017-08-15 00:00:00
abstract：:Tomato spotted wilt virus (TSWV) is a plant-infecting member of the family Bunyaviridae. TSWV encodes two envelope glycoproteins, G(N) and G(C), which are required for virus infection of the arthropod vector. Other members of the Bunyaviridae enter host cells by pH-dependent endocytosis. During this process, the glyco...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2005.01.007
更新日期：2005-06-01 00:00:00
abstract：:In adenovirus type 12 (Ad12)-induced tumor cells, in Ad12-transformed cells and in continuously passaged cell lines from these sources, the viral DNA is integrated in multiple copies, usually at a single chromosomal location. In different tumors or cell lines, the sites of integration of Ad12 DNA are all different. Ra...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(98)00131-2
更新日期：1999-01-01 00:00:00
abstract：:Adenoviruses, types 2 and 12 induce amplification of SV40 DNA sequences in cells of the SV40-transformed human newborn kidney cell line, NB-E. Similarly, integrated hepatitis B virus DNA sequences in the human hepatoma cell line, PLC/*PRF/5, and bovine papillomavirus (BPV) DNA sequences in BPV-transformed mouse cells ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(90)90079-q
更新日期：1990-08-01 00:00:00
abstract：:Serological parameters intend to describe antibody response to influenza vaccine in a population. However, there is uncertainty about the mathematical appropriateness and the biological or clinical meaning of conventionally used parameters. Theoretical considerations and exploration of a data-set of 16 studies with an...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2004.02.024
更新日期：2004-07-01 00:00:00
abstract：:We recently described a giant double stranded DNA virus called Mimivirus, isolated from amoebae, which might represent a new pneumonia-associated human pathogen. Its unique morphological and genomic characteristics allowed us to propose Mimivirus as a member of a new distinct Nucleocytoplasmic Large DNA viruses family...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2005.07.011
更新日期：2006-04-01 00:00:00
abstract：:In present study, we report the first complete genomic sequence of pig bovine viral diarrhea (BVD) virus, that of strain ZM-95, which is 12,220 nucleotides long and contains short 5' and 3' non-coding regions and one open reading frame encoding a large polyprotein with 28 potential N-glycosylation sites (Asn-X-Ser or ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2006.05.005
更新日期：2006-12-01 00:00:00
abstract：:PCR amplification of proviral DNA extracted from peripheral blood lymphocytes of three Chinese pigs (Banna minipig inbreed (BMI), Wu-Zhi-Shan pig (WZSP) and Neijiang pig (NJP)), using primers corresponding to highly conserved regions of reverse transcriptase (RT) of pol gene and nucleocapsid sequence of gag gene. PCR ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2004.05.007
更新日期：2004-10-01 00:00:00
abstract：:The biogeographical history of rabies can be reconstructed using molecular data. This work describes the genetic characterization of the Rabies virus variant that circulates in the Desmodus rotundus (vampire bat) population in an epizootic area and is transmitted to herbivorous livestock. The N and G genes of this vir...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.05.001
更新日期：2009-09-01 00:00:00
abstract：:Sendai virus is able to fuse with liposomes even without virus receptors. To determine the roles of envelope protein, hemagglutinin-neuraminidase (HN) and fusion (F) protein, in Sendai virus-liposome fusion, we treated the virus with proteases and examined its fusion with liposomes and the conditions of HN and F prote...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(98)00137-3
更新日期：1999-02-01 00:00:00
abstract：:The glycoprotein (G) is the only surface protein of the lyssavirus particle and the only viral product known to be capable of eliciting the production of neutralizing antibodies. In this study, the isolation of escape mutants resistant to monoclonal antibody (Mab) neutralization was attempted by a selection strategy e...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.04.019
更新日期：2016-07-15 00:00:00
abstract：:Cricket paralysis virus (Dicistroviridae: Cripavirus) (CrPV) naturally has a wide range of insect hosts which is reflected in its ability to infect several cultured insect cell lines. The expression of viral gene products is controlled by two kinds of internal ribosome entry site (IRES) elements, 5' and intergenic (IG...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(03)00139-4
更新日期：2003-08-01 00:00:00
abstract：:African swine fever (ASF) is a viral hemorrhagic disease with different clinical and lesional changes depending of virulence of strains/isolates and immunological status of pigs. In acute and subacute forms of ASF, severe vascular changes are present, with hemorrhages in different organs (mainly melena, epistaxis, ery...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2013.01.017
更新日期：2013-04-01 00:00:00
abstract：:Norovirus (NoV) is a major pathogenic agent of human acute viral gastroenteritis that occurs worldwide. In March 2017, a series of acute NoV-associated gastroenteritis outbreaks occurred in Hubei Province in central China. Here, we sought to better understand the main genotypes and potential evolutionary advantages of...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2018.03.016
更新日期：2018-04-02 00:00:00
abstract：:In the past few decades, dengue has spread rapidly and is an emerging disease in China. An unexpected dengue outbreak occurred in Xishuangbanna, Yunnan, China, resulting in 1331 patients in 2013. In order to obtain the complete genome information and perform mutation and evolutionary analysis of causative agent relate...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2017.06.015
更新日期：2017-06-15 00:00:00
abstract：:An Olive latent virus 1 isolate designated GM6, obtained from a Portuguese olive tree, was characterized and the coat protein gene sequenced and analysed. The purified virus particles showed to be isometric with ca. 30 nm in diameter and contained a single-stranded RNA species with ca. 3.7 kb. The dsRNA profile obtain...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2004.09.007
更新日期：2005-03-01 00:00:00
abstract：:In this review, we discuss possible models of bacteriophage-virus interactions. The first is based on the mechanism by which phages may interact indirectly with viruses. Its essence is that bacteriophage-derived nucleic acid may inhibit pathogenic virus infection. It seems that this phenomenon can be partly explained ...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2005.01.009
更新日期：2005-06-01 00:00:00
abstract：:Broad and potent human monoclonal neutralizing antibodies have considerable potential in the prevention and treatment of acquired immunodeficiency syndrome (AIDS). To identify the key amino acid recognition site contacted with neutralizing antibody Y498, peptides were panned from the PhD-12 peptide library and predict...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2020.198001
更新日期：2020-08-01 00:00:00
abstract：:The mechanism by which hepatitis B virus (HBV) infection causes severe inflammatory liver diseases is multifactorial and related to interactions with cell signaling pathways and the ensuing inflammatory response. Activation of JAK/STAT/SOCS signaling is essential for the induction of cellular antiviral responses, cont...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.12.003
更新日期：2010-03-01 00:00:00
abstract：:An account is given of progress during the last 30 years in knowledge of the epidemiology of diseases caused by aphid-borne viruses in seed potato production areas of northern Europe. During this period, Potato leafroll virus (PLRV) and strains of Potato virus Y (PVY(O), PVY(N)) were consistently the most prevalent an...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/s0168-1702(00)00186-6
更新日期：2000-11-01 00:00:00
abstract：:Viruses frequently spread among cells or hosts in groups, with multiple viral genomes inside the same infectious unit. These collective infectious units can consist of multiple viral genomes inside the same virion, or multiple virions inside a larger structure such as a vesicle. Collective infectious units deliver mul...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2019.03.013
更新日期：2019-05-01 00:00:00
abstract：:One of the essential steps in canine parvovirus (CPV) infection, the release from endosomal vesicles, is dominated by interactions between the virus capsid and the endosomal membranes. In this study, the effect of sphingomyelin and phosphatidyl serine on canine parvovirus capsid and on the phospholipase A(2) (PLA(2)) ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2007.10.008
更新日期：2008-03-01 00:00:00
abstract：:Three dendrimeric peptides were synthesized in order to evaluate their immunogenicity and their potential protection against classical swine fever virus (CSFV) in domestic pigs. Construct 1, an optimized version of a previously used dendrimer, had four copies of a B-cell epitope derived from CSFV E2 glycoprotein conne...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2017.05.020
更新日期：2017-06-15 00:00:00
abstract：:Influenza virus rgVac1sub-P0 (H5N1) (rgVac1-P0), in which a pair of dibasic amino acid residues was introduced at the cleavage site of the HA of a reassortant of H5N2 and H7N1 viruses of duck origin, was low pathogenic in chickens. Vac1sub-P3 (H5N1) (Vac1-P3) was selected as a highly pathogenic avian influenza virus b...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2013.02.001
更新日期：2013-05-01 00:00:00
abstract：:It has recently been reported that the xenotropic murine leukemia virus-related virus (XMRV) derives from a laboratory recombinant. However, sequences with characteristics of the 5' half of XMRV (termed PreXMRV-2) have been identified in several laboratory mouse genomes and cell lines suggesting parts of the XMRV geno...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.06.026
更新日期：2012-10-01 00:00:00
abstract：:The fusion (F) proteins of 10 strains of bovine respiratory syncytial virus (BRSV) were compared by radioimmunoprecipitation with fractionation on SDS-polyacrylamide gels. Two different molecular weights (15 kDa and 20 kDa) of the F2 proteins were demonstrated among the BRSV strains tested. To delineate the molecular ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(93)90059-v
更新日期：1993-08-01 00:00:00
abstract：:The 2015 Brazilian Zika virus outbreak sparked a rapid response to control the spread of the virus. What was first understood to be a mild self-resolving infection is now linked to significant neurological defects in both neonates and adults. The WHO declared the 2016 Zika epidemic a public health emergency and issued...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2017.07.025
更新日期：2018-08-02 00:00:00
abstract：:Recent reports indicated presence of two antigenic and genetic groups (genotypes VI and VII) of foot-and-mouth disease virus (FMDV) type A in India and are divergent from the vaccine strains. In order to choose suitable field isolate as candidate vaccine strain, anti-sera against representative isolates from both the ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2005.03.021
更新日期：2005-09-01 00:00:00
