Abstract：
:It has recently been reported that the xenotropic murine leukemia virus-related virus (XMRV) derives from a laboratory recombinant. However, sequences with characteristics of the 5' half of XMRV (termed PreXMRV-2) have been identified in several laboratory mouse genomes and cell lines suggesting parts of the XMRV genome exist as naturally occurring retroviruses in mice. We compare here PreXMRV-2 gag sequence diversity in mice to that of reported XMRV-like sequences by testing a panel of wild mouse and common inbred laboratory mouse strain genomic DNAs and by using high throughput amplicon sequencing. Sequences with features typical of previously reported PreXMRV-2 sequences, among them a 24 nt deletion, were repeatedly identified in different wild mice and inbred mouse strains within a high background of non-XMRV-like sequences. However, Sanger sequencing of clones from amplicons failed to retrieve such sequences effectively. Phylogenetic analysis suggests that PreXMRV-2 gag sequences from mice, cell lines and patient samples belong to the same evolutionarily young clade and that such sequences are diverse and widespread within Mus musculus domesticus and laboratory mice derived from this species. No evidence of PreXMRV-2 like gag sequences could be obtained outside of the M. musculus lineage. The results suggest that accurate determination of presence, absence and relationships of specific murine retroviral strains benefit greatly from deep sequencing analysis.
journal_name
Virus Resjournal_title
Virus researchauthors
Mayer J,Mazzoni CJ,Greenwood ADdoi
10.1016/j.virusres.2012.06.026subject
Has Abstractpub_date
2012-10-01 00:00:00pages
30-7issue
1eissn
0168-1702issn
1872-7492pii
S0168-1702(12)00223-7journal_volume
169pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract：:West Nile virus (WNV) and Japanese encephalitis virus (JEV), the members of JEV serocomplex group are pathogens of global health concern. The co-circulation of these viruses poses challenges in effective diagnostics due to antigenic similarity between the E-protein of these viruses. The present study aimed to design c...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2011.08.008
更新日期：2012-01-01 00:00:00
abstract：:Subgroup J avian leukosis virus (ALV-J) is an oncogenic retrovirus primarily causing myeloid leukosis (ML) in broilers. Although ALV is well under control in a few countries including the USA, poultry industry in many parts of the world continues suffering from serious economic loss due to sporadic or widespread ALV i...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.07.003
更新日期：2012-10-01 00:00:00
abstract：:The entry of herpes simplex virus (HSV) into an oral epithelial cell line, primary normal human oral keratinocytes (NHOK) and gingival fibroblasts (GF) was examined. Infection of these cells by HSV-1 and HSV-2 was blocked by heparin. Further examination indicated that heparin reduced viral attachment but not penetrati...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(02)00055-2
更新日期：2002-06-01 00:00:00
abstract：:Recent evidence has accumulated which definitively shows that chemokine receptors CCR5 and CXCR4 play an essential role as coreceptors for human immunodeficiency virus type 1 (HIV-1) infection. Flow cytometric analysis permitted us to detect CD38, a surface marker of early differentiation, as well as activation of T c...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(00)00220-3
更新日期：2001-01-01 00:00:00
abstract：:A recent study has shown that the reovirus lambda1 protein can unwind double-stranded nucleic acid molecules, a process energetically coupled to the hydrolysis of nucleoside 5'-triphosphates. In the present study, it was demonstrated that lambda1, expressed as a fusion protein with the Escherichia coli maltose-binding...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(97)00092-0
更新日期：1997-10-01 00:00:00
abstract：:The core proteins of epizootic haemorrhagic disease virus (EHDV) have important roles to perform in maintaining the structure and function of the virus. A complete genetic and phylogenetic analysis was therefore performed on these proteins (and the genes that code for them) to allow comparison of the selective pressur...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.07.011
更新日期：2009-11-01 00:00:00
abstract：:The main cellular target for African swine fever virus (ASFV) is the porcine macrophage. However, existing data about the early phases of infection were previously characterized in non-leukocyte cells such as Vero cells. Here, we report that ASFV enters the natural host cell using dynamin-dependent and clathrin-mediat...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2015.01.022
更新日期：2015-03-16 00:00:00
abstract：:The intercellular adhesion molecule-1 (ICAM-1) receptor was expressed in primary chicken embryo cells using a retroviral vector and shown to specifically bind major group human rhinoviruses (HRVs). A truncated, membrane-bound ICAM-1 protein containing N-terminal domains 1, 2, and 3 retained the ability to bind virus w...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(92)90005-t
更新日期：1992-07-01 00:00:00
abstract：:We have investigated the infection of Aedes albopictus (mosquito) cell clones by Sindbis virus. Variation in the multiplicity of infection (MOI) from ranges of 50-0.00005 pfu/cell was determined to have no effect on the progression of the infection to high acute phase titer, suggesting that intracellular factors alone...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(97)01461-5
更新日期：1997-07-01 00:00:00
abstract：:Human adenoviruses have been used for mammalian expression vectors and recombinant vaccines for heterologous antigens. We constructed and characterized an infectious adenovirus recombinant containing core-E1-E2 genes of hepatitis C virus (HCV). The core protein was produced mainly during the early phase of viral infec...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(98)00043-4
更新日期：1998-06-01 00:00:00
abstract：:Recombinant clones containing all or portions of the bovine papillomavirus (BPV) upstream regulatory region (URR) were constructed. When analyzed by polyacrylamide gel electrophoresis, the cloned URR sequences exhibited electrophoretic properties characteristic of DNA containing bend sites. Two distinct bend centers w...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(89)90083-x
更新日期：1989-05-01 00:00:00
abstract：:DNA clones representing the small genomic segment of Nephropathia epidemica virus strain Hällnäs B1 (NEV) and Hantaan virus strain 76-118 (HTV) encoding their nucleocapsid proteins were inserted into the E. coli vector pIN-III-ompA for secretion of proteins into the periplasmic space. The complete HTV and NEV nucleoca...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(91)90090-i
更新日期：1991-03-01 00:00:00
abstract：:Translation initiation of the coxsackievirus B3 (CVB3) RNA occurs by internal ribosomal entry. The internal ribosomal entry site (IRES) of the virus has been mapped to the 5' untranslated region (5' UTR) of the genome. As well, the 5' UTR has been suggested to play roles in determining the tissue tropism and infectivi...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(02)00138-7
更新日期：2002-12-01 00:00:00
abstract：:Viruses have evolved unique strategies and mechanisms to recruit ribosomes to ensure continued translation of their viral RNA during infection. The Dicistroviridae family of invertebrate viruses contains an unusual internal ribosome entry site (IRES), which can directly recruit ribosomes in the absence of initiation f...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2005.10.011
更新日期：2006-07-01 00:00:00
abstract：:Tobacco (cv. Xanthi nn) plants were watered with allopurinol [4-hydroxypyrazolo (3,4-d) pyrimidine, HPP], a xanthine oxidoreductase (XOR) inhibitor, to investigate its effects on infection by Tobacco mosaic virus engineered to express the green fluorescent protein (TMV.GFP). TMV.GFP infection was monitored by examinat...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.05.018
更新日期：2008-11-01 00:00:00
abstract：:A panel of 6 neutralizing monoclonal antibodies (MAbs) raised against A/Moscow/IIV01/2009 (H1N1) virus isolated during the 2009 pandemic was used for the selection of 26 escape mutants. The mutants were characterized in immune cross-reactions with the panel of MAbs. The sequencing of the mutant HA genes revealed 5 ami...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.03.003
更新日期：2012-06-01 00:00:00
abstract：:Rice false smut caused by Ustilaginoidea virens is a destructive disease in many rice-growing areas. Mycoviruses have been described in many fungal species, but there is little information regarding mycoviruses in U. virens. In this study, double-stranded (ds) RNA banding patterns were assessed in 198 wild-type isolat...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.08.022
更新日期：2015-01-02 00:00:00
abstract：:A double-stranded (ds) RNA, approximately 9.5kb in size; was identified in the MVC86 isolate of Valsa ceratosperma. Complete sequence of the dsRNA revealed a 9543-bp segment (excluding the 3' poly-A tail) that is predicted to encode a single large protein (P330). P330 has 63%, 49%, and 55% amino acid sequence identiti...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.02.008
更新日期：2012-05-01 00:00:00
abstract：:p29, a newly identified Kaposi's sarcoma-associated herpesvirus (KSHV) protein, is the product of ORF67, the positional homolog of the conserved herpesvirus protein UL34. Like its homologues in other herpesviruses, p29 is expressed early during viral lytic cycle, and is localized on the nuclear rim. Upon chemical indu...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2013.04.001
更新日期：2013-08-01 00:00:00
abstract：:The packaging of HIV genomic RNA is mediated by a specific interaction between a nucleocapsid (NC) protein and packaging signal (psi, psi) RNA sequence. However, this interaction can be inhibited by the presence of peptides or proteins that specifically bind to the psi sequence. The 125-base-long psi RNA comprises a s...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2004.05.012
更新日期：2004-11-01 00:00:00
abstract：:Five premature termination mutations and five missense mutations were introduced into the portion of cloned Moloney murine leukemia virus (M-MuLV) DNA encoding the Env cytoplasmic domain. All of the mutant DNAs gave rise to replication-competent virus after transfection of NIH/3T3 cells, but several of the mutant DNAs...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(95)01278-8
更新日期：1996-03-01 00:00:00
abstract：:An orange stem pitting citrus tristeza virus (CTV) isolate CTV-B165 was found to be symptomatically similar to other known CTV-VT isolates however molecular methods failed to classify it as an identifiable CTV genotype. The sequence variation of the Indian CTV-B165 isolate was compared to the three well known CTV geno...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2010.03.017
更新日期：2010-08-01 00:00:00
abstract：:To examine the mechanism of HIV-1 regulation by NF-IL6 in activated human cells, we selected a Jurkat cell line that did not contain endogenous NF-IL6. In this cellular environment, we evaluated the effect of exogenous NF-IL6 on transcription mediated by native and deleted LTR sequences. In Jurkat cells stimulated wit...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(02)00110-7
更新日期：2002-10-01 00:00:00
abstract：:Ranaviruses (family Iridoviridae, genus Ranavirus) have been recognized as emerging infectious pathogens and caused a great loss to the global biodiversity. Thymidine kinase (TK) and deoxyuridine triphosphatase (dUTPase, DUT, encoded by ORF 67R) are ubiquitous, existing in iridoviruses and other organisms. Previous st...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.01.008
更新日期：2016-03-02 00:00:00
abstract：:Transcription of human immunodeficiency virus (HIV-1) is activated by viral Tat protein which regulates HIV-long terminal repeat (LTR) transcription and elongation. HIV-1 Tat protein is a substrate for the deacetylase activity of sirtuin 1 (SIRT1). Here we investigate the signaling pathway involved in Tat-induced HIV-...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.08.005
更新日期：2009-12-01 00:00:00
abstract：:This review covers 35 years (1980-2014) representing a period of changing land use and agricultural practices in the United Kingdom (UK), which have also witnessed a marked change in the availability and application of new diagnostic technologies. During this period there have been 53 first records of viruses and viro...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2017.06.029
更新日期：2017-09-15 00:00:00
abstract：:For the production of viral genomic RNA, HIV-1 is dependent on an early viral protein, Tat, which is required for high-level transcription. The quantity of viral RNA detectable in blood of HIV-1 infected individuals varies dramatically, and a factor involved could be the efficiency of Tat protein variants to stimulate...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2018.04.008
更新日期：2018-05-02 00:00:00
abstract：:The capsid structures of the 7 serotypes of foot-and-mouth disease virus have been compared utilizing a series of neutralizing monoclonal antibodies which were previously shown to recognize at least 4 distinct epitopes on type A12 virus. A radioimmune binding assay using radioactively labeled antigens and the monoclon...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(86)90055-9
更新日期：1986-10-01 00:00:00
abstract：:Hepatitis D virus (HDV) is the smallest animal infecting RNA virus with unique features distinguishing it from other Hepatitis viruses. Codon usage variation is considered as an indicator of the forces shaping genome evolution. RSCU (relative synonymous codon usage) values, nucleotide contents, ENC (effective number o...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2013.01.007
更新日期：2013-05-01 00:00:00
abstract：:Previous investigations suggest that the divergence of the group I alphabaculoviruses was later than that of the group II alphabaculoviruses, however, there is no quantitative data to support this hypothesis. To examine this theory, the evolutionary rates of the 30 core genes that are conserved among all baculoviruses...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.01.015
更新日期：2009-06-01 00:00:00
