Abstract：
:We have investigated the infection of Aedes albopictus (mosquito) cell clones by Sindbis virus. Variation in the multiplicity of infection (MOI) from ranges of 50-0.00005 pfu/cell was determined to have no effect on the progression of the infection to high acute phase titer, suggesting that intracellular factors alone are responsible for the restriction of virus production seen as the infection enters the persistent phase: While persistently infected (over 1 year post infection) cell clones are morphologically indistinct from uninfected cells, they do display a uniform 30% reduction in growth rate compared with uninfected cells of the same clone. Using flow cytometry-based DNA content analysis, we found that persistent Sindbis virus infection induces distinct cytological effects on these cells, including an increase in apoptosis and polyploidy in one clone and cell cycle phase effects in another. Finally, the observation that the number of cells in persistently infected cell cultures which are productively infected closely approximates the number of cells dying by apoptosis prompted us to investigate the role that cell death may play in the maintenance of the persistent infection. Persistently infected cell cultures which were artificially induced into apoptosis by short 45 degrees C heat treatments do not display increased Sindbis virus production. This result does not support the hypothesis that infection sensitivity induced by random apoptosis in persistently infected cell cultures is responsible for the long-term maintenance of the persistent infection.
journal_name
Virus Resjournal_title
Virus researchauthors
Karpf AR,Blake JM,Brown DTdoi
10.1016/s0168-1702(97)01461-5subject
Has Abstractpub_date
1997-07-01 00:00:00pages
1-13issue
1eissn
0168-1702issn
1872-7492pii
S0168-1702(97)01461-5journal_volume
50pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract：:Recombinant clones containing all or portions of the bovine papillomavirus (BPV) upstream regulatory region (URR) were constructed. When analyzed by polyacrylamide gel electrophoresis, the cloned URR sequences exhibited electrophoretic properties characteristic of DNA containing bend sites. Two distinct bend centers w...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(89)90083-x
更新日期：1989-05-01 00:00:00
abstract：:African horse sickness (AHS) is a devastating viral disease affecting equines and has resulted in many disastrous epizootics. To date, no successful therapeutic treatment exists for AHS, and commercially used live-attenuated vaccines have various undesirable side effects. Previous studies have shown that mice inoculat...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2020.198284
更新日期：2021-01-06 00:00:00
abstract：:Orf virus infection causes a contagious pustular dermatitis characterized by extensive vascular changes that have been linked to a virally encoded vascular endothelial growth factor (VEGF). The VEGF genes of different strains of orf virus can vary extensively in amino acid sequence. Functional analyses of two major va...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2007.04.018
更新日期：2007-09-01 00:00:00
abstract：:Friesian calves were infected with one of three isolates of bovine viral diarrhoea virus (BVDV) and used to establish parameters for an in vitro model of BVDV-reactive T-cell responses in cattle. The study assessed virus clearance, seroconversion, maturation of lymphoproliferative responses (both during and following ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(00)00131-3
更新日期：2000-03-01 00:00:00
abstract：:Bovine viral diarrhea virus (BVDV) infection in cattle can lead to the development of a fatal syndrome called mucosal disease (MD). The induction of MD requires co-existence of two biotypes of BVDV: cytopathic (cp) and non-cytopathic (ncp). Studies have shown that in some cases, cp viruses are generated from ncp virus...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(98)00073-2
更新日期：1998-09-01 00:00:00
abstract：:The variability of the Apple stem pitting virus (ASPV) coat protein (CP) gene was investigated. The CP gene of ten virus isolates from apple and pear trees was sequenced. Comparison of all sequenced virus isolates revealed high diversity of the CP gene (70.7-93.5% at the nucleotide level and 77.8-98.7% at the amino ac...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2011.03.003
更新日期：2011-06-01 00:00:00
abstract：:Mosquito-borne alphaviruses are disseminated globally and cause febrile illness in humans and animals. Since the prevalence and diversity of alphaviruses has not been previously investigated in Zambia, reverse transcription PCR was employed as a broad-spectrum approach for the detection of alphaviruses in mosquitoes. ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2018.04.005
更新日期：2018-05-02 00:00:00
abstract：:The surface antigen gene region from five chronic hepatitis B virus (HBV) infected chimpanzees was amplified by PCR and the sequence determined. Sequence comparison confirmed that all of the sequences were chimpanzee hepatitis B virus (chHBV) and they appeared to represent three distinct clusters or branches. To addre...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(01)00334-3
更新日期：2001-11-05 00:00:00
abstract：:In the early 1970s, the Chinese Equine Infectious Anemia Virus (EIAV) vaccine, EIAV(DLA), was developed through successive passages of a wild-type virulent virus (EIAV(L)) in donkeys in vivo and then in donkey macrophages in vitro. EIAV attenuation and cell tropism adaptation are associated with changes in both envelo...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2007.04.005
更新日期：2007-09-01 00:00:00
abstract：:West Nile virus (WNV) and Japanese encephalitis virus (JEV), the members of JEV serocomplex group are pathogens of global health concern. The co-circulation of these viruses poses challenges in effective diagnostics due to antigenic similarity between the E-protein of these viruses. The present study aimed to design c...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2011.08.008
更新日期：2012-01-01 00:00:00
abstract：:Transplacental viral infections are dependent upon complex interactions between feto-placental and maternal immune responses and the stage of fetal development at which the infection occurs. Bovine viral diarrhea virus (BVDV) has the ability to cross the placenta and infect the fetus. Infection early in gestation with...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.05.018
更新日期：2012-08-01 00:00:00
abstract：:The Herpes simplex virus type-1 (HSV-1) regulatory protein ICP0, a RING-finger E3 ubiquitin ligase, stimulates the onset of viral lytic replication and the reactivation of quiescent viral genomes from latency. Like many ubiquitin ligases ICP0 induces its own ubiquitination, a process that can lead to its proteasome-de...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.05.017
更新日期：2008-10-01 00:00:00
abstract：:It has been previously reported that the fp25K product of Bombyx mori nucleopolyhedrovirus (BmNPV) is required for post-mortem host degradation, but the mechanism by which it regulates host degradation is still unknown. This study shows that disruption of BmNPV fp25K attenuates the expression of viral cathepsin gene (...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.11.014
更新日期：2009-03-01 00:00:00
abstract：:Recent reports indicated presence of two antigenic and genetic groups (genotypes VI and VII) of foot-and-mouth disease virus (FMDV) type A in India and are divergent from the vaccine strains. In order to choose suitable field isolate as candidate vaccine strain, anti-sera against representative isolates from both the ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2005.03.021
更新日期：2005-09-01 00:00:00
abstract：:An orange stem pitting citrus tristeza virus (CTV) isolate CTV-B165 was found to be symptomatically similar to other known CTV-VT isolates however molecular methods failed to classify it as an identifiable CTV genotype. The sequence variation of the Indian CTV-B165 isolate was compared to the three well known CTV geno...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2010.03.017
更新日期：2010-08-01 00:00:00
abstract：:Although African swine fever virus (ASFV) replicates in viral cytoplasmic factories, the presence of viral DNA within the host cell nucleus has been previously reported to be essential for productive infection. Herein, we described, for the first time, the intranuclear distribution patterns of viral DNA replication ev...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2015.07.006
更新日期：2015-12-02 00:00:00
abstract：:Hepatitis E is acute hepatitis caused by infection of hepatitis E virus (HEV) via a fecal-to-oral or zoonotic route. HEV is a small, non-enveloped virus containing positive strand RNA as a genome. Recently, the three-dimensional structures of the HEV-like particles and spike domain protruded from the surface of the pa...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2011.03.015
更新日期：2011-10-01 00:00:00
abstract：:Felid herpesvirus 1 (FHV-1) mutants were constructed using two-step Red-mediated recombination techniques based on a virulent full-length FHV-1 BAC clone. The individual mutant viruses generated were deficient in glycoprotein C (gC), glycoprotein E (gE), US3 serine/threonine protein kinase (PK), or both gE and thymidi...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.05.001
更新日期：2016-08-02 00:00:00
abstract：:A simple and sensitive method for the detection of measles virus genome was developed, amplifying the regions encoding the nucleocapsid (N) protein and hemagglutinin (H) protein of measles virus by reverse transcriptase-polymerase chain reaction (RT-PCR). We examined a variety of measles patients: 28 patients with nat...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(94)00074-m
更新日期：1995-01-01 00:00:00
abstract：:In order to clarify the genetic relationships and dynamics of rabies viruses that are epidemic in Indonesia, we determined and analyzed 1307 nucleotides of nucleoprotein genes of 34 rabies field isolates collected from Sumatra, Java, Kalimantan, Sulawesi and Flores islands. Results of phylogenetic analysis indicated t...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.03.001
更新日期：2008-07-01 00:00:00
abstract：:The biogeographical history of rabies can be reconstructed using molecular data. This work describes the genetic characterization of the Rabies virus variant that circulates in the Desmodus rotundus (vampire bat) population in an epizootic area and is transmitted to herbivorous livestock. The N and G genes of this vir...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.05.001
更新日期：2009-09-01 00:00:00
abstract：:During 2014-2015, 270 fecal samples were collected from non-diarrheic, captive and wild African green monkeys (AGMs) on the island of St. Kitts, Caribbean region. By RNA-PAGE, picobirnaviruses (PBVs) were detected in sixteen captive AGMs. By RT-PCR and sequencing of partial gene segment-2, PBVs in 15 of these 16 sampl...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.12.021
更新日期：2017-02-15 00:00:00
abstract：:In the present study, we show that infection with infectious bursal disease virus (IBDV) causes activation of macrophages, the key cells involved in inflammatory and immune-regulatory functions. Exposure of cultured spleen macrophages (SM) from SPF chickens to IBDV resulted in the production of nitric oxide (NO). In a...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2005.11.015
更新日期：2006-06-01 00:00:00
abstract：:The glycoprotein (G) is the only surface protein of the lyssavirus particle and the only viral product known to be capable of eliciting the production of neutralizing antibodies. In this study, the isolation of escape mutants resistant to monoclonal antibody (Mab) neutralization was attempted by a selection strategy e...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.04.019
更新日期：2016-07-15 00:00:00
abstract：:Adenoviruses, types 2 and 12 induce amplification of SV40 DNA sequences in cells of the SV40-transformed human newborn kidney cell line, NB-E. Similarly, integrated hepatitis B virus DNA sequences in the human hepatoma cell line, PLC/*PRF/5, and bovine papillomavirus (BPV) DNA sequences in BPV-transformed mouse cells ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(90)90079-q
更新日期：1990-08-01 00:00:00
abstract：:It was previously reported that during serial passage of a BHK-derived cell line persistently infected with foot-and-mouth disease virus, (termed C1-BHK-Rc1) the virus became increasingly more virulent for BHK-21 cells (de la Torre et al 1988). Virus strains isolated from different cell passage levels were tested for ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(90)90084-o
更新日期：1990-12-01 00:00:00
abstract：:The packaging of HIV genomic RNA is mediated by a specific interaction between a nucleocapsid (NC) protein and packaging signal (psi, psi) RNA sequence. However, this interaction can be inhibited by the presence of peptides or proteins that specifically bind to the psi sequence. The 125-base-long psi RNA comprises a s...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2004.05.012
更新日期：2004-11-01 00:00:00
abstract：:The 'infectious DNA' approach, which is based on in vivo transcription of (+)RNA virus genome cDNA cassettes from eukaryotic promoters in transfected cells, became a popular alternative to the classical scheme in the infectious clone methodology. Its use, however, is often limited by the instability of plasmids due to...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(01)00371-9
更新日期：2001-12-04 00:00:00
abstract：:Japanese encephalitis is a reproductive disorder caused by Japanese encephalitis virus (JEV) in swine. Previous studies have demonstrated that recombinant adenovirus serotype 5 (Ad5) may be a potential vaccine candidate because it can express JEV envelope epitopes and induce immune responses against JEV. Still, it wil...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.05.025
更新日期：2016-08-15 00:00:00
abstract：:Thogoto (THO) virus infections of Rhipicephalus appendiculatus ticks were examined using tick hosts immune to the virus. In the first set of experiments, ticks were infected by feeding on viraemic hamsters. Inter-stadial infection of THO virus was not affected when ticks ingested a virus-immune bloodmeal but there was...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(89)90034-8
更新日期：1989-10-01 00:00:00
