Abstract:
:In the present study, we show that infection with infectious bursal disease virus (IBDV) causes activation of macrophages, the key cells involved in inflammatory and immune-regulatory functions. Exposure of cultured spleen macrophages (SM) from SPF chickens to IBDV resulted in the production of nitric oxide (NO). In addition, there was upregulation of mRNA expression of inducible nitric oxide synthase (iNOS), IL-8 and cyclooxygenase-2 (COX-2). The signal transduction pathways involved in macrophage activation were examined. The role of mitogen-activated protein kinases (MAPKs) and nuclear factor-kappaB (NF-kappaB) was tested by using specific pharmacological inhibitors. Addition of p38 MAPK inhibitor, SB-203580 and NF-kappaB inhibitor Bay 11-7082, suppressed IBDV-induced NO production and mRNA expression of iNOS, IL-8 and COX-2. The results suggest that IBDV uses cellular signal transduction machinery, in particular the p38 MAPK and NF-kappaB pathways, to elicit macrophage activation. The increased production of NO, IL-8 and COX-2 by macrophages may contribute to bursa inflammatory responses commonly seen during the acute IBDV infection.
journal_name
Virus Resjournal_title
Virus researchauthors
Khatri M,Sharma JMdoi
10.1016/j.virusres.2005.11.015subject
Has Abstractpub_date
2006-06-01 00:00:00pages
70-7issue
1-2eissn
0168-1702issn
1872-7492pii
S0168-1702(05)00354-0journal_volume
118pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract::Citrus psorosis (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV) belong to the family Ophioviridae, plant viruses with filamentous nucleocapsids and segmented genomes of negative polarity, causing the worldwide distributed citrus psorosis and lettuce big-vein diseases, respectively. To gain insight into the replic...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.08.005
更新日期:2012-12-01 00:00:00
abstract::A disease of Rudbeckia hirta (Black-eyed Susan), characterized by severe flower deformation, was observed in Minnesota during 2010-2016. A previously undescribed virus species, named Rudbeckia flower distortion virus (RuFDV, family Caulimoviridae, genus unassigned), was determined to be the causal agent of the disease...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2017.05.012
更新日期:2017-09-15 00:00:00
abstract::The transforming potential of DNA tumor viruses derives mainly from the ability of their encoded oncogene products to interact with cellular proteins. Many of these viral oncoproteins share regions of sequence similarity, designated conserved region 1 and 2, which have been implicated in complex formation with pRb, th...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(96)01404-9
更新日期:1997-01-01 00:00:00
abstract::From the sera of patients with advanced cancer, a novel factor called SDF (serum-derived factor) was partially purified. SDF was shown to stimulate transcription from the long terminal repeat (LTR) of human immunodeficiency virus type 1 (HIV-1) by transient CAT assay. It did not stimulate gene expression of various co...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(93)90065-u
更新日期:1993-09-01 00:00:00
abstract::MicroRNAs (miRNAs) play important roles in viral infections, especially by modulating the expression of cellular factors essential to viral replication or the host innate immune response to infection. To identify host miRNAs important to controlling porcine reproductive and respiratory syndrome virus (PRRSV) infection...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2014.08.012
更新日期:2015-01-02 00:00:00
abstract::The diagnosis of infectious diseases is sometimes difficult because of extensive immunological cross-reactivity between related viral antigens. On the path of constructing sero-specific antigens, we have identified residues involved in sero-specific and cross-reactive recognition of the nucleocapsid proteins (NPs) of ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2008.06.003
更新日期:2008-10-01 00:00:00
abstract::The measles virus induces a life-long immune response associated with antibodies specific for the fusion protein. To map the linear immunodominant recognition sites of the fusion (F) protein of the measles virus, we have reacted a complete set of 108 overlapping pentadecapeptides with purified IgG obtained from donor ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(93)90095-5
更新日期:1993-12-01 00:00:00
abstract::Hand, foot and mouth disease (HFMD) in humans is caused mainly by Enterovirus 71(EV71) and Coxsackievirus A16 (CVA16). EV71 is associated with severe HFMD cases but not CVA16. Use of IgM-capture enzyme-linked immunosorbent assay (ELISA) is important for the early diagnosis of EV71 infection, but cross-reactivity of th...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2013.04.015
更新日期:2013-09-01 00:00:00
abstract::Despite the fact that cells infected with wild type vaccinia virus synthesize viral DNA and assemble progeny virus particles within the cytoplasm, the host cell nucleus is required for a productive infection. Recent evidence suggests that vaccinia virus selectively recruits components from the host cell nucleus into t...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/0168-1702(87)90014-1
更新日期:1987-09-01 00:00:00
abstract::A series of recombinant fusion proteins derived from equine arteritis virus (EAV) open reading frame (ORF) 7 have been used to define the immunoreactive region of the viral nucleocapsid (N) protein. Reactivities of recombinant N fusion proteins with post-infection equine sera in immunoblots and ELISAs indicate that th...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(95)00098-4
更新日期:1995-12-01 00:00:00
abstract::Our understanding of picornavirus RNA replication has improved over the past 10 years, due in large part to the discovery of cis-active RNA elements (CREs) within picornavirus RNA genomes. CREs function as templates for the conversion of VPg, the Viral Protein of the genome, into VPgpUpU(OH). These so called CREs are ...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2008.07.027
更新日期:2009-02-01 00:00:00
abstract::Vesicular exanthema of swine virus (VESV), the prototype calicivirus, is the etiologic agent of the porcine disease vesicular exanthema of swine (VES). VES is characterized by vesicle formation on the extremities, mouth and snout and causes abortions and stillbirths if infection occurs during pregnancy. VESV is consid...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(98)00013-6
更新日期:1998-03-01 00:00:00
abstract::Thogoto (THO) virus infections of Rhipicephalus appendiculatus ticks were examined using tick hosts immune to the virus. In the first set of experiments, ticks were infected by feeding on viraemic hamsters. Inter-stadial infection of THO virus was not affected when ticks ingested a virus-immune bloodmeal but there was...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(89)90034-8
更新日期:1989-10-01 00:00:00
abstract::Equine rhinitis A virus (ERAV) is a picornavirus which causes an acute respiratory infection in horses worldwide, and virus neutralization (VN) has been the standard method for the detection of ERAV antibody in horse serum. Previous studies have identified recombinant virion protein VP1 (rVP1) purified under native co...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2009.03.003
更新日期:2009-07-01 00:00:00
abstract::We have studied which steps are enhanced in the infectious cycle of influenza A virus in Madin-Darby canine kidney (MDCK) cells, a cell line investigated for use in the production of an influenza vaccine because of its ability to yield high levels of virus. We have confirmed that MDCK had the highest production levels...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2008.04.028
更新日期:2008-09-01 00:00:00
abstract::Severe Combined Immune Deficiency mouse tumors, induced by inoculating peripheral blood mononuclear cells from 11 healthy human donors (hu-PBMC-SCID tumors), were used to analyse Epstein-Barr virus (EBV) type and strain variations. PCR analysis of EBNA 2- and EBNA 3C-specific sequences showed that EBV type A was prese...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(95)00005-b
更新日期:1995-05-01 00:00:00
abstract::Broad and potent human monoclonal neutralizing antibodies have considerable potential in the prevention and treatment of acquired immunodeficiency syndrome (AIDS). To identify the key amino acid recognition site contacted with neutralizing antibody Y498, peptides were panned from the PhD-12 peptide library and predict...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2020.198001
更新日期:2020-08-01 00:00:00
abstract::Human papillomavirus (HPV) infection leads to aneuploidy, a numerical chromosomal aberration that is caused by dysregulation of chromosomal segregation. We previously found that the E7 proteins of high-risk HPVs, but not of low-risk HPVs, could bind to centromere protein-C (CENP-C). In this study, we first found that ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2015.04.019
更新日期:2015-07-02 00:00:00
abstract::Following viral infection, the host responds by mounting a robust anti-viral response with the aim of creating an unfavorable environment for viral replication. As a countermeasure, viruses have elaborated mechanisms to subvert the host response in order to maintain viral protein synthesis and production. In the last ...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2012.06.004
更新日期:2012-11-01 00:00:00
abstract::With the aim to detect what kind of cells, in addition to erythroid progenitors, could be involved in the pathogenesis of B19 infection in some connective tissue diseases, primary cultures of human fibroblasts (HF) and endothelial cells (HUVEC) were exposed to a B19 positive serum (350 genome copies/cell). The presenc...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2005.05.003
更新日期:2005-12-01 00:00:00
abstract::Plant viruses express RNA silencing suppressor (RSS) proteins to counteract plant defence mechanisms. Here, we describe a method to assess the RSS activity based on an alfalfa mosaic virus (AMV) RNA 3 expression vector and transgenic Nicotiana tabacum plants that express the P1 and P2 subunits of the AMV replicase (P1...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2019.197733
更新日期:2019-10-15 00:00:00
abstract::The paramyxo- and pneumoviruses are members of the order Mononegavirales, a group of viruses with non-segmented, negative strand RNA genomes. The polymerases of these viruses are multi-functional complexes, capable of transcribing subgenomic capped and polyadenylated mRNAs and replicating the genome. Although there is...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2017.01.008
更新日期:2017-04-15 00:00:00
abstract::Influenza A viruses are characterized by a unique genome structure, causing genetic instability, especially to the genes of haemagglutinin and neuraminidase. The objectives of this research was molecular and phylogenetic analysis of influenza A(H3N2) strains that circulated in Northern Greece since 2004, particularly ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2009.07.006
更新日期:2009-11-01 00:00:00
abstract::Confocal microscopy revealed that Rice dwarf virus (RDV) initially accumulated in epithelial cells of the filter chamber of leafhopper vector Nephotettix cincticeps 2 days after acquisition access feeding on diseased plants. Subsequently, RDV accumulation progressed to the anterior midgut, and then spread to the nervo...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2011.04.028
更新日期:2011-09-01 00:00:00
abstract::Noroviruses belong to the Caliciviridae family of single-stranded positive-sense RNA viruses. The genus Norovirus includes seven genogroups (designated GI-GVII), of which GI, GII and GIV infect humans. Human noroviruses are responsible for widespread outbreaks of acute gastroenteritis and represent one of the most com...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2016.12.018
更新日期:2017-04-15 00:00:00
abstract::The main objective of this narrative review is to describe the available evidence on the possible antiviral activity of ozone in patients with COVID-19 and its therapeutic applicability through hospital protocols. Amongst different possible therapies for SARS-CoV-2 pneumonia, ozone therapy seems to have an immunologic...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2020.198207
更新日期:2021-01-02 00:00:00
abstract::The fusion (F) proteins of 10 strains of bovine respiratory syncytial virus (BRSV) were compared by radioimmunoprecipitation with fractionation on SDS-polyacrylamide gels. Two different molecular weights (15 kDa and 20 kDa) of the F2 proteins were demonstrated among the BRSV strains tested. To delineate the molecular ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(93)90059-v
更新日期:1993-08-01 00:00:00
abstract::Rat theilovirus (RTV) is a cardiovirus related to Theiler's murine encephalomyelitis virus. While RTV is a prevalent viral pathogen of rats used in biomedical research, the pathogenesis and characterization of RTV infections is not well understood. In the studies reported herein, we used immunohistochemistry to identi...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2011.07.014
更新日期:2011-09-01 00:00:00
abstract::The fidelity of poliovirus RNA-dependent RNA polymerase (3Dpol) was determined using a modified alpha-complementation assay. Several polymerases have been analyzed by this approach allowing comparisons to be drawn. Various conditions including high and low MgCl(2), replacing MgCl(2) with MnCl(2), skewed nucleotide poo...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2009.10.006
更新日期:2010-01-01 00:00:00
abstract::It has been previously reported that the fp25K product of Bombyx mori nucleopolyhedrovirus (BmNPV) is required for post-mortem host degradation, but the mechanism by which it regulates host degradation is still unknown. This study shows that disruption of BmNPV fp25K attenuates the expression of viral cathepsin gene (...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2008.11.014
更新日期:2009-03-01 00:00:00