Abstract:
:The fidelity of poliovirus RNA-dependent RNA polymerase (3Dpol) was determined using a modified alpha-complementation assay. Several polymerases have been analyzed by this approach allowing comparisons to be drawn. Various conditions including high and low MgCl(2), replacing MgCl(2) with MnCl(2), skewed nucleotide pools, and the presence of poliovirus protein 3AB were analyzed. The assay included RNA synthesis by 3Dpol on an RNA template that coded for a region of the alpha peptide of beta-galactosidase (lacZ-alpha). The product of this reaction was used as a template for a second round of 3Dpol synthesis and the resulting RNA was reverse transcribed to DNA by reverse transcriptase. The DNA was amplified by PCR and inserted into a vector used to transform Escherichia coli. The bacteria were screened for beta-galactosidase activity by blue-white phenotype analysis with white or faint blue colonies scored as errors made during synthesis on lacZ-alpha. Although 3AB strongly stimulated 3Dpol synthesis as expected, no change in fidelity was detected. Changes in MgCl(2) also showed little effect. Mutation rates of approximately 9 x 10(-5) (approximately 1 error per 11,000 incorporations) were estimated for these conditions. In contrast, MnCl(2) or skewed nucleotide pools were highly mutagenic resulting in lowered fidelity.
journal_name
Virus Resjournal_title
Virus researchauthors
DeStefano JJdoi
10.1016/j.virusres.2009.10.006subject
Has Abstractpub_date
2010-01-01 00:00:00pages
53-9issue
1eissn
0168-1702issn
1872-7492pii
S0168-1702(09)00355-4journal_volume
147pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract::Application of bacteriophages and their lytic enzymes to control Clostridium perfringens is one potential approach to reduce the pathogen on poultry farms and in poultry-processing facilities. Bacteriophages lytic for C. perfringens were isolated from sewage, feces and broiler intestinal contents and ΦCPV1, a virulent...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2010.11.012
更新日期:2011-02-01 00:00:00
abstract::Orf virus infection causes a contagious pustular dermatitis characterized by extensive vascular changes that have been linked to a virally encoded vascular endothelial growth factor (VEGF). The VEGF genes of different strains of orf virus can vary extensively in amino acid sequence. Functional analyses of two major va...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2007.04.018
更新日期:2007-09-01 00:00:00
abstract::Reports of human infections with highly pathogenic H5N1 avian influenza viruses in many countries in Asia and Africa with varying case fatality rates highlight the pandemic potential of these viruses. In order to contain a rapidly spreading influenza virus in a pandemic scenario, a vaccine which can induce rapid and r...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2013.09.013
更新日期:2013-12-26 00:00:00
abstract::Transcription of human immunodeficiency virus (HIV-1) is activated by viral Tat protein which regulates HIV-long terminal repeat (LTR) transcription and elongation. HIV-1 Tat protein is a substrate for the deacetylase activity of sirtuin 1 (SIRT1). Here we investigate the signaling pathway involved in Tat-induced HIV-...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2009.08.005
更新日期:2009-12-01 00:00:00
abstract::Echovirus 18 (EV18) is one of the echovirus serotypes associated with human diseases and in particular aseptic meningitis. To facilitate studies of the molecular epidemiology of EV18 and the evolution of enteroviruses in general, the complete nucleotide (nt) sequence was determined for the echovirus 18 prototype strai...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(02)00019-9
更新日期:2002-04-23 00:00:00
abstract::A panel of 6 neutralizing monoclonal antibodies (MAbs) raised against A/Moscow/IIV01/2009 (H1N1) virus isolated during the 2009 pandemic was used for the selection of 26 escape mutants. The mutants were characterized in immune cross-reactions with the panel of MAbs. The sequencing of the mutant HA genes revealed 5 ami...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.03.003
更新日期:2012-06-01 00:00:00
abstract::In situ hybridization and virus titration were used to characterize early stages of rat virus (RV) infection of rat pups after oronasal inoculation. Results suggest that virus enters through the lung and that early viremia leads rapidly to pantropic infection. Cells derived from all three germ layers were infected wit...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(93)90085-2
更新日期:1993-04-01 00:00:00
abstract::Human recombinant alpha interferon, A/D, significantly reduced the replication and cell fusion induced by herpes simplex virus type 1 in monkey cells. Thin-section electron microscopy of interferon-treated monkey cells showed distinct assembly of nucleocapsids within the nucleus. Analysis of virus-specific proteins by...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(89)90051-8
更新日期:1989-01-01 00:00:00
abstract::The Sindbis viral expression system enables the rapid production of high levels of recombinant protein in mammalian cells; however, this expression is typically limited to transient production due to the cytotoxicity of the virus. Limiting the lethality inherent in the Sindbis virus vector in order to enable long term...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2008.12.019
更新日期:2009-04-01 00:00:00
abstract::A putative nucleoside triphosphate phosphohydrolase (NTPase) gene of African swine fever virus was identified by using a degenerate oligonucleotide probe derived from the nucleoside triphosphate binding motif, which is highly conserved among viral and cellular NTPases. The probe hybridized with fragments SalI E and Ec...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(93)90016-g
更新日期:1993-10-01 00:00:00
abstract::Real-time and visual tracking of viral infection is crucial for elucidating the infectious and pathogenesis mechanisms. To track the virus successfully, an efficient labeling method is necessary. In this review, we first discuss the practical labeling techniques for virus tracking in live cells. We then describe the c...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2017.05.007
更新日期:2017-06-02 00:00:00
abstract::Bovine herpesvirus-1 infected cell protein 27 (BICP27) was detected predominantly in the nucleolus. The open reading frame of BICP27 was fused with the enhanced yellow fluorescent protein (EYFP) gene to investigate its subcellular localization in live cells and BICP27 was able to direct monomeric, dimeric or trimeric ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2009.07.024
更新日期:2009-11-01 00:00:00
abstract::We previously prepared neutralizing monoclonal antibody (MAb)-resistant (mar) mutant viruses using a laboratory strain feline infectious peritonitis virus (FIPV) 79-1146 (Kida et al., 1999). Mar mutant viruses are mutated several amino acids of the neutralizing epitope of Spike protein, compared with the parent strain...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2010.12.020
更新日期:2011-03-01 00:00:00
abstract::Consensus patterns of baculovirus sequences upstream from the translational initiation sites have been analyzed and a web tool, Local Alignment Promoter Predictor (LAPP), for the prediction of baculovirus promoter sequences has also been developed. Potential consensus sequences, i.e., TCATTGT, TCTTGTA, CTCGTAA, TCCATT...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2005.04.016
更新日期:2005-10-01 00:00:00
abstract::Cowpox virus (CPXV) host range factor CP77 was identified to be required for virus replication in Chinese hamster ovary (CHO) cells, but the underlying molecular mechanism by which CP77 modulates host range has remained unclear. Therefore, a CPXVΔCP77 deletion mutant was constructed by applying bacterial artificial ch...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.06.005
更新日期:2012-09-01 00:00:00
abstract::An Olive latent virus 1 isolate designated GM6, obtained from a Portuguese olive tree, was characterized and the coat protein gene sequenced and analysed. The purified virus particles showed to be isometric with ca. 30 nm in diameter and contained a single-stranded RNA species with ca. 3.7 kb. The dsRNA profile obtain...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2004.09.007
更新日期:2005-03-01 00:00:00
abstract::The interaction between UV-B irradiation and cutaneous human papillomaviruses (HPV) has been suggested to be of relevance for the development of non-melanoma skin cancers. We investigated the activity within the upstream regulatory region (URR) of the HPV types 8, 38, 92, 93 and 96, as well as their responsiveness to ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2008.07.013
更新日期:2008-11-01 00:00:00
abstract::Human papillomavirus (HPV) infection leads to aneuploidy, a numerical chromosomal aberration that is caused by dysregulation of chromosomal segregation. We previously found that the E7 proteins of high-risk HPVs, but not of low-risk HPVs, could bind to centromere protein-C (CENP-C). In this study, we first found that ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2015.04.019
更新日期:2015-07-02 00:00:00
abstract::Sendai virus is able to fuse with liposomes even without virus receptors. To determine the roles of envelope protein, hemagglutinin-neuraminidase (HN) and fusion (F) protein, in Sendai virus-liposome fusion, we treated the virus with proteases and examined its fusion with liposomes and the conditions of HN and F prote...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(98)00137-3
更新日期:1999-02-01 00:00:00
abstract::Porcine deltacoronavirus (PDCoV), a newly discovered enteric coronavirus, is a causative agent of severe clinical diarrhea and intestinal pathological damage in piglets. As a first step toward understanding the effect of PDCoV on host cells, we elucidated mechanisms underlying the process of apoptotic cell death after...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2018.06.008
更新日期:2018-07-15 00:00:00
abstract::Despite the fact that cells infected with wild type vaccinia virus synthesize viral DNA and assemble progeny virus particles within the cytoplasm, the host cell nucleus is required for a productive infection. Recent evidence suggests that vaccinia virus selectively recruits components from the host cell nucleus into t...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/0168-1702(87)90014-1
更新日期:1987-09-01 00:00:00
abstract::The DNA polymerase from Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) was identified and sequenced, and its amino acid sequence was compared with other viral DNA polymerases to identify conserved regions and to reconstruct a phylogenetic tree. The sequence analysis of the AgMNPV DNA polymerase gene revealed the ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2005.01.010
更新日期:2005-06-01 00:00:00
abstract::Since 2002 there has been a rise in arthrogryposis/hydranencephaly (AGH) incidence in Israel, caused by Akabane (AKA) and, possibly, Aino viruses. To test the ability to control the disease, three siRNA genes targeted to the S genome segment were designed and prepared in the form of siRNA cassettes. For the design all...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2006.02.009
更新日期:2006-09-01 00:00:00
abstract::Infectious salmon anaemia virus (ISAV) is an orthomyxovirus causing anaemia and circulatory disease with high mortality in farmed Atlantic salmon (Salmo salar). Orthomyxoviruses are unusual as RNA viruses as they replicate in the nucleus and some viral transcripts undergo splicing. The nuclear replication necessitates...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2013.07.001
更新日期:2013-10-01 00:00:00
abstract::Apoptosis is frequently induced to inhibit virus replication during infection of Enterovirus 71 (EV71). On the contrary, anti-apoptotic pathway, such as PI3K/Akt pathway, is simultaneously exploited by EV71 to accomplish the viral life cycle. The relationship by which EV71-induced apoptosis and PI3K/Akt signaling path...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2014.07.026
更新日期:2014-11-04 00:00:00
abstract::Autophagy is an evolutionarily conserved lysosome-dependent degradation pathway that acts in the maintenance of cellular homeostasis and plays important functions in viral replication and pathogenesis. In this study, we investigated the role of autophagy in the replication of porcine reproductive and respiratory syndr...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2011.11.008
更新日期:2012-02-01 00:00:00
abstract::Marek's disease virus (MDV) is an oncogenic herpesvirus that causes a disease in chickens characterized by tumor formation and immunosuppression. In this paper, we identified differentially expressed proteins in the bursa of Fabricius of chickens infected with the highly virulent MDV strain RB1B, using two-dimensional...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2010.08.010
更新日期:2010-11-01 00:00:00
abstract::Borna disease virus grows to low titres in persistently infected cells with an infectious particle to cell ratio of 0.01 to 0.05. Inclusion of n-butyrate in the growth medium enhances infectivity yields up to 1 log. This effect is time and concentration dependent. In hypertonic medium with an excess of NaCl, KCl or Na...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(85)90057-7
更新日期:1985-02-01 00:00:00
abstract::Five strains of Coxsackie B4 virus and one of Echo 11 virus were tested with regard to their ability to replicate in pancreatic mouse beta-cells and interfere with the alterations of the cytoplasmic Ca2+ concentration ([Ca2+]i) induced by glucose. All strains except one both multiplied and caused cytopathic effect. In...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(94)90105-8
更新日期:1994-09-01 00:00:00
abstract::Lepidopteran nucleopolyhedroviruses have distinct viral tissue tropisms in host larvae. We previously identified the Bm8 gene of Bombyx mori nucleopolyhedrovirus (BmNPV), the product of which inhibits viral propagation in the middle silk gland (MSG). However, it is unknown whether this inhibitory function of the Bm8 p...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2018.03.011
更新日期:2018-04-02 00:00:00